• The Korean Journal of Physiology
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• v.29 no.2
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• pp.259-267
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• 1995

The renin-angiotensin system plays an important role in the regulation of blood pressure and in body fluid homeostasis. There is increasing evidence for generation of endogenous angiotensin II in many organs and for its role in paracrine functions. Studies were designed to investigate whether hemorrhage produces rapid changes in the gene expression of angiotensinogen in peripheral and brain tissues. Wistar rats received saline drinking water for 7 days, were bled at a rate of $3\;ml\;kg^{-1}\;min^{-1}$ for 7 min, and then decapitated 0, 2, 4, 8, or 24 hr after hemorrhage. Hemorrhage produced a produced hypotension with tachycardia at $2{\pm}8\;hr$, but blood pressure and heart rate had not fully recovered to the basal level at 24 hr. Plasma renin concentration was significantly increased at 2, 4, and 8 hr (maximum sixfold increase at 4 hr) and had returned to the basal level at 24 hr. Renal renin content was significantly increased only at 4 hr after hemorrhage. Angiotensinogen mRNA in both the kidney and liver were stimulated at 2 to 8 hrs, but recovered to the basal level at 24 hr. On the other hand, angiotensinogen mRNA levels il the hypothalamus and brainstem were continuously increased from 2 to 24 hrs. The present study demonstrates the presence of angiotensinogen mRNA in both hepatic and extrahepatic tissues, and more importantly, their up-regulation after hemorrhage. These results suggest that the angiotensinogen-generating systems in the liver, kideny and brain are, at least in part, under independent control and play a local physiological role.

• Journal of The Korean Society of Agricultural Engineers
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• v.50 no.3
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• pp.31-41
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• 2008

There are many agricultural facilities (46.7%) that have been over 30 years in the Republic of Korea (the ratio of reservoirs built before 1945 is 53%, from 1946 to 1971 is 35%). In the case of reservoirs which do most important functions among these facilities, only 3,000 reservoirs are managed by Korea Rural Community & Agriculture Corporation (KRC) and the other 15,000 reservoirs are managed by local authorities (City and Gun). But, 15,000 reservoirs included in City. Gun have been built in more wide area than KRC and the reservoir management system has not been operated well because of shortage of budget and manpower of the local authorities. Particularly, the abnormal weather happened during every summer season recently has ruined a lot of rural community facilities. So flood control function have been more important than irrigation function in agricultural reservoir system and it may be required to introduce new safe, management and maintenance techniques in City Gun reservoirs. So we investigated landscapes and deteriorations of agricultural reservoirs managed by local small governments for revealing not only present usage but also future value. Survey shows that there are no structural managements except typical hydrological reportings and the deterioration of small darn is very serious. There are needs for more systematic management system and equipment methods. Therefore, this study may suggest that reservoir (that takes charge of the greatest deal of weight in all agricultural facilities) must be considered as new concept of the usage and the unification. On the other hand, reservoir must be also developed as amenity resources, natural circumstances and district values.

• Reproductive and Developmental Biology
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• v.38 no.1
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• pp.53-62
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• 2014

The objective of this study was to investigate the relationship between fertility and protein pattern change using in vitro fertilization, analysis of sperm characteristics and two-dimensional gel electrophoresis in different pig types. In results, the viability and mitochondria integrity of sperm were higher significantly (p<0.05) but the portions of acrosome reaction was lower significantly (p<0.05) in Duroc and $F_1$ (potbellied ${\times}$ PWG miniature pig) than PWG miniature. On in vitro fertilization to investigate fertility, the fertility of $F_1$ semen war higher significantly (p<0.05) than in Duroc and PWG miniature pig. On the other hand, protein patterns showed similar function among the different boar semen. Especially, the heat shock 70 kDa 1-like and G patch domain-containing protein 4 were significantly (p<0.05) higher expressed in $F_1$ than in Duroc and PWG miniature pig. The proteins associated with mitochondria in Duroc were significantly (p<0.05) higher expressed than in $F_1$ and PWG miniature pig. The developmental rates to blastocyst stage of oocytes fertilized with sperm of $F_1$ pig were significantly (p<0.05) higher than in PWG miniature pig. However, phosphoglycerate kinase 2 and zinc finger protein 431 were significantly (p<0.05) higher expressed in PWG miniature pig than in $F_1$ and Duroc pigs. In conclusion, the results of the present study indicate that different proteins were expressed in different pig types, and were associated with a sperm functions and embryo development.

• Journal of Embryo Transfer
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• v.29 no.2
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• pp.133-139
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• 2014

$K^+$ channels are involved in the regulation of a variety of physiological functions, including proliferation, apoptosis and differentiation, in mammalian cells. Our previous study demonstrated that the blockage of $K^+$ channels inhibits mouse early embryonic development. This study was designed to identify the effect of $K^+$ channels during bovine embryonic development. $K^+$ channel blockers (tetraethylammonium (TEA), $BaCl_2$, quinine, ruthenium red and fluoxetine) were added to the culture medium during in vitro fertilization (IVF) for 6 h to first identify the short-term effect of these chemicals. Among $K^+$ channel blockers, fluoxetine, which is used as a selective serotonin reuptake inhibitor, significantly increased the blastocyst formation rate by approximately 6% when compared to control. During the in vitro maturation (IVM) of immature oocytes and the in vitro culture (IVC) of embryos, the oocytes and embryos were exposed to fluoxetine for either a short-term (6 h) or a long-term (24 h) to compare the embryonic development in response to exposure time. The 6 h exposure to fluoxetine during IVM did not affect the blastocyst formation rate, but the rate of blastocyst formation was reduced after the 24 h exposure. On the other hand, embryonic development increased approximately 10% in both groups of embryos exposed to fluoxetine for 6 and 24 h during IVC. Taken together, fluoxetine treatment during IVF and IVC, but not IVM, enhances bovine embryonic development. These results suggest that fluoxetine-modulated signals in oocytes and embryos could be an important factor towards enhancing bovine embryonic development.

• The Journal of the Korea Contents Association
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• v.18 no.9
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• pp.535-543
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• 2018

The traditional culture, which is a historical product possessed by each country, represents the competitiveness of the country and becomes a national resource. Raw lacquer has a very long history and tradition in China. Lacquer, which is the basic material of Raw lacquer. Also it is a natural paint which has the functions of preservation, insectproof and waterproof used from ancient times in Asia, not only in China but also in Korea and Japan. Such Raw lacquer using lacquer has a long history and it is becomes to a tradition. On the other hand, Its value and meaning are being faded by oilpaintings and watercolors. Much effort and research are needed to become more popular. I would like to introduce the lacquering technique in illustration as one way. There are a lots of expression techniques in the present illustration, but lacquer expression is rarely seen. In the illustration, lacquering technique is a modern reinterpretation of traditional culture by combining modern technology with traditional culture. In this paper, a Raw lacquer with a long tradition of soul is informed to the public and it is a way to bring attention to the beauty of lacquer and reproduce the original expression technique as an illustration to enhance the understanding of traditional culture, I hope that Raw lacquer will be used in the field.

• The Journal of the Korea Contents Association
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• v.20 no.10
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• pp.726-737
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• 2020

This study analyzed 4,284 elderly people aged 65 or older by SPSS 22.0 in the 7th data of 「2018 Korean Longitudinal Study of Ageing(KLoSA)」 to confirm the effect of the health-promoting behavior of the elderly on life satisfaction through cognitive function. The cognitive function level of the subjects was 13.8% doubtful about dementia, 23.8% cognitive decline, and 62.4% normal, and the satisfaction level of life was 59.304 points. The results of the study were as follows: First, the difference in cognitive function according to demographic characteristics was significant in gender, age, education, religion, and marriage factors. Second, the satisfaction of the elderly's life according to demographic characteristics was confirmed to have significant differences in gender, age, education, religion, and marriage factors. Third, the satisfaction of the elderly's life was significantly positive in drinking and regular exercise factors among independent variable the health-promoting behavior when cognitive function was mediated. On the other hand, The significant negative effect of the restriction of daily life activities was confirmed. In this way, the mediating effect of cognitive function was verified in the effect of the health-promoting behavior on the satisfaction of the elderly life.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.18 no.7
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• pp.350-356
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• 2017

With increasing attention to real-time body detection, active research is being conducted on human body detection based on skin color. Despite this, most existing skin detection methods utilize static skin color models and have detection rates in images, in which colors are distorted. This study proposed a method of detecting the skin region using a fuzzy classification of the gradient map, saturation, and Cb and Cr in the YCbCr space. The proposed method, first, creates a gradient map, followed by a saturation map, CbCR map, fuzzy classification, and skin region binarization in that order. The focus of this method is to rigorously detect human skin regardless of the lighting, race, age, and individual differences, using features other than color. On the other hand,the borders between these features and non-skin regions are unclear. To solve this problem, the membership functions were defined by analyzing the relationship between the gradient, saturation, and color features and generate 108 fuzzy rules. The detection accuracy of the proposed method was 86.35%, which is 2~5% better than the conventional method.

• Asian-Australasian Journal of Animal Sciences
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• v.20 no.5
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• pp.795-801
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• 2007

Patients with Type I diabetes mellitus have been treated with porcine insulin for several decades and pigs have recently been deemed an ideal source of microencapsulated islet cells for clinical xenotransplantation. In this study, neonatal pigs were anesthetized and sacrificed prior to a pancreatectomy. Islet cells were isolated from pancreas via collagenase digestion. Islet cells were separated and collected by hand under microscopic guidance. These cells were suspended in 1.4% sodium alginate solution and encapsulated by dropping them into 1.1% calcium chloride solution and in which the round gel in size was 250-400 ${\mu}m$ in diameter. Viability of the microencapsulated islet cells cultured in medium at $37^{\circ}C$ was assessed by MTT assay. Furthermore, insulin released in response to glucose challenge was investigated using an enzyme-linked immunosorbent assay. Secretion of insulin was low in response to the basal glucose solution (4.4 mM) in medium and was significantly higher in response to the high glucose solution (16.7 mM). The viability of microencapsulated islet cells did not differ significantly over a period of 7 days; that is, the increasing pattern of insulin concentration in the culture medium after glucose stimulation interval day was similar throughout the 7 days cultivation. In summary, experimental evidences indicated that the effects of alginate-microencapsulation prolonged survival of the neonatal porcine islets in vitro cultures and the insulin response to glucose of the islets was maintained.

• Asian Pacific Journal of Cancer Prevention
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• v.15 no.6
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• pp.2439-2445
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• 2014

Cryptotanshinone (CPT), is a quinoid diterpene isolated from the root of the Asian medicinal plant, Salvia miotiorrhiza bunge. Numerous researchers have found that it could work as a potent antitumor agent to inhibit tumor growth in vitro, buith there has been much less emphasis on its in vivo role against breast tumors. Using a mouse tumor model of MCF7 cells, we showed that CPT strongly inhibited MCF7 cell growth in vivo with polarization of immune reactions toward Th1-type responses, stimulation of naive CD4+ T cell proliferation, and also increased IFN-${\gamma}$ and perforin production of CD4+ T cells in response to tumor-activated splenocytes. Furthermore, data revealed that the cytotoxic activity of CD4+ T cells induced by CPT was markedly abrogated by concanamycin A(CMA), a perforin inhibitor, but not IFN-${\gamma}$ Ab. On the other hand, after depletion of CD4+ T cells or blocked perforin with CMA in a tumor-bearing model, CPT could not effectively suppress tumor growth, but this phenomenon could be reversed by injecting naive CD4+ T cells. Thus, our results suggested that CPT mainly inhibited breast tumor growth through inducing cytotoxic CD4+ T cells to secrete perforin. We further found that CPT enhanced perforin production of CD4+ T cells by up-regulating JAK2 and STAT4 phosphorylation. These findings suggest a novel potential therapeutic role for CPT in tumor therapy, and demonstrate that CPT performs its antitumor functions through cytotoxic CD4+ T cells.

• Korean Journal of Human Ecology
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• v.18 no.5
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• pp.1093-1101
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• 2009

To provide basic data to manufacture superior brassieres, we performed a survey on the wearing and buying habits, and preference of functions and materials of brassieres for 189 women subjects in their twenties. The results of this research are as follows; Style and size are the most important in purchasing brassieres. Flat breasted and sagging breasted subjects were dissatisfied in the fit of the upper cup. 70% of the subjects were ignorant of their brassiere size. Sagging breasted subjects were less satisfied with smoothness, softness, and weight when wearing their bras in comparison with the other types. Conical type breasts were common amongst women in their 20s. They are more likely to wear size 75A and 80A where the full bust girth is 10cm larger than the under-bust. They preferred demi cup brassieres with thin or moderate padding to full cup brassieres with thick padding. On the other hand, 56.8% of subjects wore brassieres all day. The subjects were dissatisfied with the slipping down of the shoulder straps. Large-breasted subjects were concerned more with dampness and the fit on the center front of the brassiere, and brassieres with too thick padding had a poor fit on the center front. Sports-brassieres which fit close to the skin and used stretchable fabric caused more itching and dampness and dissatisfaction in removal of the brassiere.