• 생명과학회지
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• 제17권1호
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• pp.137-142
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• 2007

Monascus purpureus KFRI 1134 균주에 대해 UV ($235_{nm}$, 30 second) 조사를 통한 변이주 형성을 유도하여 Monascus sp. MK2-2를 분리하였다. 홍국 천연색소 생산의 최적조건은 rice powder 0.3%와 0.2% yeast extract, 0.3% $NH_4H_2PO_4$의 배지 조성에서 초기 pH 5.0, 배양온도 $30^{\circ}C$ 및 배양기간은 5일이었으며, 홍국 천연색소 생산에 미치는 탄소원의 영향은 wheat flour > rice powder > fructose 순으로 높았다. 질소원은 sodium nitrate > $KNO_3$의 순서로 생성능이 높았으며, C/N ratio가 $17\sim22$일 때 색소의 생성능이 우수하였다. Anti-thrombosis activity 측정결과 Monascus sp. MK2-2 추출물은 aspirin과 유사한 항혈전 기능을 지니고 있음을 알 수 있었다.

• Applied Biological Chemistry
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• 제13권3호
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• pp.231-235
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• 1970

1. 내열성 사상균 Humicola속의 Amylase를 정제하였다. 2. DEAE-Cellulose Column Chromatography로 2개의 활성구분으로 나누어 졌다. 3. 즉 pH 6.0, 0.05M와 0.5M의 인산염완충용액으로 용출시킬때 전자에서 당화형, 후자에서 호정화형 Amylase가 각각 분리되었다. 4. 이 당화형 Amylase의 최적 pH는 $4.5{\sim}5.5$이고, 안정 pH 범위는 $4.0{\sim}9.0$범위였다. 최적온도는 $60{\sim}65^{\circ}C$로 다른 사상균의 그것보다 훨씬높고 $80^{\circ}C$ 이상에서는 불활성화 되었다.

• Journal of Microbiology and Biotechnology
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• 제9권3호
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• pp.235-242
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• 1999

We investigated the growth-inhibitory mechanism of Helicobacter pylori by omeprazole (OMP) and its activated sulfenamide (OAS). Using dithiothreitol (DTT) and 5,5'-dithio-bis[2-nitrobenzoic acid] (DTNB; Ellman's reagent), we first determined the relationship between the binding capacity of these compounds to H. pylori membrane and its significance to membrane P-type ATPase activity. After incubation of the intact H. pylori cells with either OMP or OAS, the residual quantity of free SH-groups on the cell membrane was measured, and, the resulting values were plotted as a function of time. From this experiment, we found that there was a considerable difference in the membrane-binding rates between OMP and OAS. At neutral pH, the disulfide bond formation on H. pylori membrane was completed within 2 min of incubation of the intact cells with OAS. By OMP, however, it was gradually formed, exceeding 10 min of incubation for completion, whereby, the extent of P-type ATPase inhibition appeared to be proportional to the disulfide forming rate. From this data, it was suggested that the disulfide formation might directly affect enzyme activity. Since OMP per se cannot yield a disulfide bond with cysteine, it is predicted that the enzyme inactivation must be caused by the OAS form. Accordingly, we postulated that, under the neutral pH, OMP could be converted to OAS in the course of transport. By extrapolating the inhibitory slopes, we could evaluate K₁ values, relating to their minimal inhibitory concentrations (MICs) for H. pylori growth. In these MIC ranges, H. pylori uptake or vesicular export of nutrients such as peptides were totally prohibited, but their effect in Escherichia coli were negligible. From these observations, we strongly suggest that the P-type ATPase activity is essential for the survival of H. pylori cells in particular.

• 한국수학교육학회지시리즈D:수학교육연구
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• 제14권3호
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• pp.195-209
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• 2010

Utilizing a structure of operations known as Dissection-Motion-Operations (DMO), a set of mathematics propositions or area-formulas in school mathematics will be introduced through shape-to-shape transforms. The underlying theme for DMO is problem-solving through visual reasoning and proving manipulatively or electronically vs. rote learning and memorization. Visual reasoning is the focus here where two operations that constitute DMO are utilized. One operation is known as Dissection (or Decomposition) operation that operates on a given region in 2D or 3D and dissects it into a number of subregions. The second operation is known as Motion (or Composition) operation applied on the resultant sub-regions to form a distinct area (or volume)-equivalent region. In 2D for example, DMO can transform a given polygon into a variety of new and distinct polygons each of which is area-equivalent to the original polygon (cf [Rahim, M. H. & Sawada, D. (1986). Revitalizing school geometry through Dissection-Motion Operations. Sch. Sci. Math. 86(3), 235-246] and [Rahim, M. H. & Sawada, D. (1990). The duality of qualitative and quantitative knowing in school geometry, International Journal of Mathematical Education in Science and Technology 21(2), 303-308]).

• 대한수학회논문집
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• 제10권1호
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• pp.235-249
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• 1995

Let K be a bounded linear operator from Hilbert space $H_1$ into Hilbert space $H_2$. When numerically solving the first kind equation Kf = g, one usually picks n orthonormal functions $\phi_1, \phi_2,...,\phi_n$ in $H_1$ which depend on the numerical method and on the problem, see Varah [12] for more details. Then one findes the unique minimum norm element $f_M \in M$ that satisfies $\Vert K f_M - g \Vert = inf {\Vert K f - g \Vert : f \in M}$, where M is the linear span of $\phi_1, \phi_2,...,\phi_n$. Such a solution $f_M \in M$ is called the M-solution of K f = g. Some methods for finding the M-solution of K f = g were proposed by Banks [2] and Marti [9,10]. See [5,6,8] for convergence results comparing the M-solution of K f = g with $f_0$, the least squares solution of minimum norm (LSSMN) of K f = g.

• 한국지반공학회:학술대회논문집
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• 한국지반공학회 1993년도 지하공간 건설기술에 관한 서울 심포지움 논문집
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• pp.235-247
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• 1993

The 62 m span Olympic lee Hockey cavern in Gjovik, Norway, is located in jointed gneiss of average RaD = 70% and has a rock cover of only 25 to 50m, thus posing challenging design p problems. The investigations prior to construction included two types of stress measurements, cross-hole seismic tomography, special coe logging, Q-system classification and numerical modelling with UDEC-BB. Predicted maximum deformations were 4 to 8 mm; surprisingly small due to the high horizontal stresses recorded. Extensometer (MPBX) installations from the surface prior to construction, precision surface levelling and MPBX installed from inside the cavern give a combined measure of maximum deformations in the range 7 to 8 mm with the 62 m span fully e excavated, and three adjacent caverns for the Postal Services also completed.

• 미생물학회지
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• 제38권4호
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• pp.235-240
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• 2002

식품폐수처리장의 활성슬러지와 논, 밭의 토양에서 우수한 protease 생성 균주을 분리 선별하였다. 이 중 효소활성이 우수한 PB16은 그람 음성, 간균이며 protease activity는 6.49 U/ml이었다. 생리, 생화학적 특성 및 16S rRNA 염기서열분석을 실시한 결과, Aeromonas hydrophila (99.0%) 인 것으로 확인 되었다. Bioscreen C를 사용한 최적 성장조건 평가는 합성폐수에 vitamin과mineral을 첨가한 배지의 중식속도(0.26 $h^{-1}$)가 무 첨가배지(0.21 $h^{-1}$)보다 높았으며, 분리균주의 합성폐수 유기물 제거능 실험에서는 초기 soluble-CODcr 2,472 mg/l인 고농도 합성폐수를 1일, 3일 반응 후 각각 59, 87%의 제거율을 나타내었다.

• 한국식품과학회지
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• 제21권2호
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• pp.235-237
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• 1989

플라스틱 필름의 투습도 측정장치를 제작하여 전자투습도 측정장치로 필름의 투습도를 측정해 본 결과 두 측정 장치간에는 5% 수준(P<0.05)에서 유의차가 없었다. 따라서 제작한 투습도 측정장치를 산업에 활용할 수 있을 것으로 판단되었다.

• 열처리공학회지
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• 제18권4호
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• pp.235-241
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• 2005

Thermal creep properties of the advanced zirconium fuel claddings named by HANA alloys which were developed for high burn-up application were evaluated. The creep test of HANA cladding tubes was carried out by the internal pressurization method in temperature range from 350 to $400^{\circ}C$ and in the hoop stress range from 100 to 150 MPa. Creep tests were lasted up to 800 days, which showed the steady-state secondary creep rate. The creep resistance of HANA fuel claddings was affected by final annealing temperature and various factors, such as alloying element, applied stress and testing temperature. From the results the microstructure observation of the samples before and after creep test by using TEM, the dislocation density was increased in the sample of after creep test. The Sn as an alloying element was more effective in the creep resistance than other elements such as Nb, Fe, Cr and Cu due to solute hardening effect of Sn. In case of HANA fuel claddings, the improved creep resistance was obtained by the control of final heat treatment temperature as well as alloying element.

• 대한수학회지
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• 제59권2호
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• pp.235-254
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• 2022

Let ℍⁿ be the Heisenberg group and Q = 2n + 2 be its homogeneous dimension. Let 𝓛 = -∆_ℍⁿ + V be the Schrödinger operator on ℍⁿ, where ∆_ℍⁿ is the sub-Laplacian and the nonnegative potential V belongs to the reverse Hölder class $B_{q_1}$ for q₁ ≥ Q/2. Let H^p_𝓛(ℍⁿ) be the Hardy space associated with the Schrödinger operator 𝓛 for Q/(Q+𝛿₀) < p ≤ 1, where 𝛿₀ = min{1, 2 - Q/q₁}. In this paper, we consider the Hardy type estimates for the operator T_𝛼 = V^𝛼(-∆_ℍⁿ + V )^-𝛼, and the commutator [b, T_𝛼], where 0 < 𝛼 < Q/2. We prove that T_𝛼 is bounded from H^p_𝓛(ℍⁿ) into L^p(ℍⁿ). Suppose that b ∈ BMO^𝜃_𝓛(ℍⁿ), which is larger than BMO(ℍⁿ). We show that the commutator [b, T_𝛼] is bounded from H¹_𝓛(ℍⁿ) into weak L¹(ℍⁿ).