• Journal of Microbiology and Biotechnology
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• v.15 no.6
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• pp.1267-1272
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• 2005

The effects of coexpression of GroEL/ES and DnaK/DnaJ/GrpE chaperones on the productivity of the soluble form of human granulocyte colony stimulating factor (hG-CSF) in E. coli were examined. Recombinant hG-CSF protein was coexpressed with DnaK/DnaJ/GrpE or GroEL/ES chaperones under the control of the araB or Pzt-1 promoter, respectively. The optimal concentration of L-arabinose for the expression of DnaK/DnaJ/GrpE was found to be 1 mg/ml. When L-arabinose was added at $OD_{600}$=0.2 (early-exponential phase), soluble hG-CSF production was greatly increased. In addition, it was observed that the DnaK/DnaJ/GrpE and GroEL/ES chaperones had no synergistic effects on preventing aggregation of hG-CSF protein. Consequently, by coexpression of the DnaK/DnaJ/GrpE chaperone, the signal intensity of the hG-CSF protein band in the soluble fraction of cell lysate was increased from $3.5\%\;to\;13.9\%$, and Western blot analysis also revealed about a 4-5-fold increase of production of soluble hG-CSF over the non-induction case of DnaK/DnaJ/GrpE.

• Food Science of Animal Resources
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• v.21 no.4
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• pp.374-382
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• 2001

Lactoferrin(Lf) has the function of modulation in the host defense systems, including cytokine production and immune responses. We have tested the effect of Lf and Lf ydrolysates(Lf-H) on the productions of tumor necrosis factor-${\alpha}$(TNF-${\alpha}$) and nitric oxide(NO) in macrophage cells. Lf from Korean native cattle(K-Lf) and hydrolyzed K-Lf(K-Lf-H) increased the production of TNF-${\alpha}$ in RAW264.7 cells with dose-dependency. Bovine Lf(B-Lf), human Lf(H-Lf), and its hydrolysates did not induce either TNF-${\alpha}$ production or NO production. On the other hand those didn\`t affect on the production of TNF-${\alpha}$ in lipopolysaccharide(LPS)-stimulated RAW264.7 cells. K-Lf induced the production of NO similar to its role on the TNF-${\alpha}$ production.

• Biomedical Science Letters
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• v.15 no.1
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• pp.37-45
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• 2009

Insect cell cultures have become important tools in the production of biological substances for use in a variety of research, human and veterinary medicine, and pest control applications. These applications often require the introduction of foreign DNA into the cells and have generally used methods originally developed for use with human and other mammalian cell cultures. While these methods can be successfully employed, they are often less efficient with insect cells and frequently involve complex procedures or require specialized equipment. Even when they do work, they may require substantial modification because of differences in the culture medium or growth patterns of insect cells. In this study, We have optimized transfection conditions of Sf9 cell line using insect expression vector pIZT/V5-His which expresses green fluorescent protein effectively. Human stem cell factor (hSCF) is a glycoprotein that plays a key role in hematopoiesis acting both as a positive and negative regulator, often in synergy with other cytokines. It also plays a key role in mast cell development, gametogenesis, and melanogenesis. It can exist in membrane-bound form and in proteolytically released soluble form. As determined by an enzyme-linked immunosorbent assay performed, hSCF level in supernatant averaged 995ng/ml. The human hSCF was partially purified by immunoaffinity chromatography and analyzed with sodium dodecyl sulfate-polyacrylamide gel electrophoresis and immunoblotting. The results show that the hSCF has N-linked carbohydrate and corresponds to the soluble form, at or about 223 amino acids in length. The findings suggest functional importance for soluble hSCF in cells.

• Analytical Science and Technology
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• v.19 no.6
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• pp.544-552
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• 2006

Emission tests were carried out to investigate the characteristics of concentration according to flooring sort using small chamber method. The target Volatile Organic Compounds (VOC) included 27 individual compounds of environmental concern, which were determined by adsorption sampling and thermal desorption coupled with GC/MS method and by DNPH cartridge/HPLC method. The emission factor of Total Volatile Organic Compounds (TVOC) and Formaldehyde (HCHO) was detected $0.3mg/m^2{\cdot}h$ and $0.2mg/m^2{\cdot}h$ respectively, and the floorings of 37 (9 PVC Tile, 10 PVC Sheet, 18 Flooring) were satisfied emission standard. TVOC emission factor appeared in order of concentration of PVC Sheet, PVC Tile, and floor flooring, while HCHO was detected very high emission factor (as $0.4mg/m^2{\cdot}h$) at floor flooring above PVC series (as $0.001mg/m^2{\cdot}h$).

• Journal of the Korean Society for Library and Information Science
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• v.46 no.2
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• pp.245-260
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• 2012

This study aims to analyze the change in the correlation between the index values and qualitative quantitative evaluation elements by applying 'the decile of total number of articles' instead of the average annual number of articles in Kor-Factor2, and to evaluate the distinction ability between registered journals and register nominee journals of the KCI. The correlation between the index values and qualitative quantitative evaluation elements was analyzed by calculating the correlation coefficient between the index values and the evaluation elements, and then the correlation was compared with that of Kor-Factor2. To evaluate the distinction ability, we carried out independent samples t-test on the mean difference between index values of register nominee journal group and registered journal group and compared the mean difference with that of Kor-Factor2, IF and journal h-index. As a result, we found that the modified index applying the decile of total number of articles was effective in improving the balance among the correlation between the index values and qualitative quantitative evaluation elements compared to Kor-Factor2, and the new index appeared to be superior to other indexes on distinction ability of the journal's registration condition in the KCI.

• Korean Journal of Food Science and Technology
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• v.40 no.5
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• pp.497-502
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• 2008

The volatile components in Gamdongchotmoo kimchi, unfermented and fermented for 3 or 25 days, were extracted via solvent-assisted flavor evaporation (SAFE), and then analyzed via gas chromatography/mass spectrometry (GCMS). A total of 57 components, including 14 S-containing compounds, 22 terpene hydrocarbons, 13 aliphatic hydrocarbons, 4 alcohols, and 4 miscellaneous components, were detected in Gamdongchotmoo kimchi. Among them, the S-compounds were quantitatively dominant. The aroma-active compounds were also determined via gas chromatography-olfactometry (GC-O), using aroma extract dilution analysis (AEDA). A total of 16 aroma-active compounds were detected via GC-O. The most intense aroma-active compounds in Gamdongchotmoo kimchi included 4-isothiocyanato-1-butene ($Log_3$ FD factor 7, rancid), an unknown($Log_3$ FD factor 7, spicy) and another unknown ($Log_3$ FD factor 7, seasoning-like). In addition, other aroma-active compounds, including dimethyldisulfide ($Log_3$ FD factor 6, rotten onion-like/sulfury), 2-vinyl-[4H]-1,3-dithiin ($Log_3$ FD factor 5, spicy/garlic-like), and an unknown ($Log_3$ FD factor 5, rancid/cheese-like) might be crucial to the flavor characteristics of Gamdongchotmoo kimchi.

• Journal of radiological science and technology
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• v.32 no.2
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• pp.205-212
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• 2009

The response correction factor ( h) is a factor to convert the response of the chamber in solid phantom to the response in water. In RW3 solid phantom, the dependency of beam quality and depth for high energy X-rays are known characteristics, however the dependency of field size, SSD, and chamber type are unknown. In this work we have studied the unknown characteristics on the dependency of response correction factor. The farmer type chamber (FC65G) and small chamber (CC13) were used and two beam qualities of 6 and 15 MV were evaluated. The measured response correction factors at the depth of 5 cm and 10 cm were h = 1.015 and 1.021 for 6 MV X-rays, and h = 1.024 and 1.029 for 15 MV X-rays. In conclusion the response correction factor did not depend on the field size and SSD while depending on the beam quality and depth. In the chambers, there are small differences between the two chambers used in this study but we think additional study for more chambers should be required. The results in this study can be used for analyzing the measured values from ionization chamber dosimetry in RW3.

• Korean Journal of Adult Nursing
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• v.21 no.6
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• pp.593-602
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• 2009

Purpose: This study was to provide baseline data about nurses' Influenza A (H1N1) knowledge, awareness, and practice of infection control and to identify the significant factor affecting the level of practice. Methods: The subjects of this study were 144 nurses who worked at Influenza A (H1N1) regional base Hospital in D city. Data were collected by self-reported questionnaires during September 2009. The collected data were analyzed using SPSS/WIN 12.0 program. Results: The knowledge of Influenza A (H1N1) was statistically different according to age, unit, career and experience of seasonal influenza vaccination during the last year. The awareness of infection control was statistically different according to age, career, experience of seasonal influenza vaccination for last year and intention to get seasonal influenza vaccination for this year. The practice of infection control was statistically different according to unit, experience of seasonal influenza vaccination for last year, intention to get seasonal influenza vaccination for this year and intention to get Influenza A (H1N1) vaccination for this year. There was positive correlation among knowledge, awareness and practice (p < .05). Awareness was the significant factor affecting the level of practice. Conclusion: An educational program focusing on strategy to change nurse's awareness can be effective for infection control of Influenza A (H1N1) in regional base hospitals.

• New & Renewable Energy
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• v.2 no.3
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• pp.31-36
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• 2006

Superstrate pin amorphous silicon thin-film(a-Si:H) solar cells are prepared on $SnO_2:F$ and ZnO:Al transparent conducting oxides(TCO) in order to see the effect of TCO/p-layers on a-Si:H solar cell operation. The solar cells prepared on textured ZnO:Al have higher open circuit voltage VOC than cells prepared on $SnO_2:F$. Presence of thin microcrystalline p-type silicon layer(${\mu}c-Si:H$) between ZnO:Al and p a-SiC:H plays a major role by causing improvement in fill factor as well as $V_{OC}$ of a-Si:H solar cells prepared on ZnO:Al TCO. Without any treatment of pi interface, we could obtain high $V_{OC}$ of 994mV while keeping fill factor(72.7%) and short circuit current density $J_{SC}$ at the same level as for the cells on $SnO_2:F$ TCO. This high $V_{OC}$ value can be attributed to modification in the current transport in this region due to creation of a potential barrier.

• Asian Pacific Journal of Cancer Prevention
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• v.13 no.8
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• pp.3899-3903
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• 2012

B7-H3 is a newly discovered member of the B7/CD28 superfamily which functions as an important T-cell immune molecule. It has been reported recently that B7-H3 is highly expressed in many cancer cells, the data indicating that it may be a regulation factor contributing to tumor-resistance. In our study, we used bioinformatics to identify differentially expressed genes between colonic cancer cells and normal colonic cells, aiming to analyze mechanisms and identify sub-pathways closely related to progression, with the final aim of finding small molecule drugs which might interfere this progression. We found that ajmaline is one related factor which may enhance self-immunity in colon carcinoma therapy and B7-H3 plays important roles with regard to immunoreactions of colonic cancer cells. All the results indicate that H7-B3 is a favorable prognostic biomarker for colon carcinomas, providing novel information regarding likely targets for intervention.