• Parasites, Hosts and Diseases
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• v.59 no.1
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• pp.47-53
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• 2021

Echinostoma mekongi was reported as a new species in 2020 based on specimens collected from humans in Kratie and Takeo Province, Cambodia. In the present study, its metacercarial stage has been discovered in Filopaludina martensi cambodjensis snails purchased from a local market nearby the Tonle Sap Lake, Pursat Province, Cambodia. The metacercariae were fed orally to an experimental hamster, and adult flukes were recovered at day 20 post-infection. They were morphologically examined using light and scanning electron microscopes and molecularly analyzed by sequencing of their mitochondrial cox1 and nad1 genes. A total of 115 metacercariae (1-8 per snail) were detected in 60 (60.0%) out of 100 Filopaludina snails examined. The metacercariae were round, 174 ㎛ in average diameter (163-190 ㎛ in range), having a thin cyst wall, a head collar armed with 37 collar spines, and characteristic excretory granules. The adult flukes were elongated, ventrally curved, 7.3 (6.4-8.2)×1.4 (1.1-1.7) mm in size, and equipped with 37 collar spines on the head collar (dorsal spines in 2 alternating rows), being consistent with E. mekongi. In phylogenetic analyses, the adult flukes showed 99.0-100% homology based on cox1 sequences and 98.9-99.7% homology based on nad1 sequences with E. mekongi. The results evidenced that F. martensi cambodjensis snails act as the second intermediate host of E. mekongi, and hamsters can be used as a suitable experimental definitive host. As local people favor to eat undercooked snails, these snails seem to be an important source of human infection with E. mekongi in Cambodia.

• Journal of the Korean Institute of Rural Architecture
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• v.23 no.4
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• pp.21-29
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• 2021

The purpose of this study is to present the basic direction of the desirable spatial plan for elderly care facilities by analyzing the spatial characteristics of elderly care facilities in gyeongnam-province. As a research method, 189 elderly care facilities in gyeongnam-province were investigated for location conditions, site area, floor area, total floor area, and space composition. In addition, the area of each specific space was analyzed for two elderly care facilities with a typical floor plan. The results of the survey and analysis are as follows. First, the average number of people in the facility was 53.8, and the number of people in the facility needs to be planned to be medium or less so that they can live in a more comfortable environment in the future. Second, in the location type, it was found that many facilities were distributed in the order of suburban and rural type, urban type, mountain type, and waterfront type. In addition, in terms of facility space composition, the proportion of detached facilities was 50.8%. Complex facilities require a reasonable plan in consideration of the location type and other functional facility characteristics. Third, the average total floor area per person of elderly care facilities was 27.57m². There is a limit to constructing a unique nursing space. In the composition of bedrooms, quadruple rooms account for 67.4%, but it is necessary to expand the proportion of bedrooms that can be used by fewer people.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.42 no.2
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• pp.116-122
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• 2009

This study was conducted to compare the food quality of commercial salt-fermented silver-stripe round herring (SFS) to that of commercial salt-fermented anchovy (SFA). The contents of moisture and crude protein of SFS were higher than those of SFA. However, the crude lipid and crude ash contents of SFS were lower than those of SFA. The salinity of SFS was 13.2%, which was lower than that of SFA. The taste value of SFS was 148.1, which was higher than that of SFA. The total amino acid content of SFS was 18.1 g/100 g and its major amino acids were glutamic acid (9.5%) and aspartic acid (8.1%). The contents of calcium, phosphorus and iron in SFS were 647 mg/100 g, 363 mg/100 g and 4.1 mg/100 g, respectively. Twenty six types of fatty acids were detected in SFS and the major components were 16:0, 18:0, 18:1n-9 and 22:6n-3. The angiotensin I converting enzyme (ACE) inhibiting and the antioxidative abilities of SFS were 50.0% and 1.15, respectively, which were similar to those of SFA. Sensory evaluation revealed that SFS had a superior taste and color to SFS, while to the flavor of two products was similar.

• The Plant Pathology Journal
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• v.22 no.1
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• pp.21-27
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• 2006

We immunized BALB/c mice with purified Cucumber green mottle mosaic virus isolate HY1 (CGMMV-HY1). Through the selection of positive clones that were grown on the HAT medium, four sensitive monoclonal clones (CG99-01, CG99-02, CG99-03, and CG99-04) were selected from 500 Hypoxanthine-guanine phosphoribosyltransferase positive hybridoma cells. Four sensitive clones of CGMMV-HYI were determined as IgM type of the subclass of mouse immunoglobulins Ig group. The titer of monoclonal antiserum against CGMMVHY1 was estimated 1:12,800 by the indirect ELISA. Although monoclonal antibodies (MAbs) from CG99-01 and from CG99-04 cross-reacted with Zucchini green mottle mosaic virus and Kyuri green mottle mosaic virus, MAb from the cell line CG99-03 was highly specific to CGMMV. No MAbs cross-reacted with Cucumber mosaic virus-Fny. Only CG99-04 reacted with Pepper mild mottle virus weakly and CG99-02 reacted with both CGMMV and KGMMV. CGMMV was detected from the rind of watermelon fruit by DAS-ELISA of CGMMV-HY1, but not from the flesh of watermelon. Average seed transmission rate of CGMMV in watermelon was $24\%$ from symptomatic watermelon collected from 5 regions of Gyeongnam province. CGMMV was detected by DAS-ELISA with specific MAb of CGMMVHY1 periodically from root stock, during the sequential process for nursery seedling in Haman. Necrotic spots on cotyledons of root stock seedling progressed to reveal the typical symptomatology on the primary leaves of scion upon grafting. Here, we have established MAb based ELISA system, which could accurately detect CGMMV from watermelon seeds, nursery seedlings, transplants and field samples from greenhouse or open out door field as well.

• Journal of Environmental Health Sciences
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• v.32 no.4 s.91
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• pp.316-323
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• 2006

This study was performed to assess the microbial contamination level for the processing of sandwich products in the middle of Gyeongnam province from December 2004 to January 2005. A total of 85 samples were collected from 5 sandwich shops. These samples were tested sanitary indication bacteria, such as aerobic Plate count(APC), coliforms, and Escherchia coli and pathogenic bacteria such as Escherichia coli O157:H7, Salmonella spp., Listeria monocytogenes, and Staphylococcus aureus. As a result of APC and coliform count ranged 0-4.59 $log_{10}$ CFU/(ml, g, 100 $cm^2$, hand) and 0-3.86 $log_{10}$ CFU/(ml, g, 100 $cm^2$, hand), respectively. Especially, the highly contaminated items for APC were confirmed 1.64-4.59 $log_{10}$ CFU/g to employees', raw materials and sandwich in all items. Escherichia coli was isolated from 5 samples. Listeria monocytogenes and Staphylococcus aureus were detected in 1 sample and 11 samples from utensil, raw materials and sandwiches, respectively. However, Escherichia coli O157:H7 and Salmonella spp. were not detected in anywhere. For the production of safety sandwich, education of sanitation for employees, control of raw materials, and continuous monitoring for microorganism will be required.

• Journal of Digital Convergence
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• v.17 no.12
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• pp.67-75
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• 2019

This study was conducted to analyze the effects of mathematics classes using engineering tools on students' mathematics academic achievement and mathematics learning attitude, focusing on the basic figure and drawing sections of the first grade of middle school. Eighty students of first-grade at H Middle School in South Gyeongsang province were divided into two groups, taking a total of six weeks of classes using engineering tools(Algeomath) and traditional tools, and covariate analysis(ANCOVA) was used to analyze students' mathematics academic achievements and changes in mathematical learning attitude. The analysis found that classes using engineering tools were effective in students' mathematics academic achievements and attitude of learning math. Based on the results of the study, the necessity of utilizing various engineering tools in the future secondary school math class and the prospect and implications of the classes were discussed.

• Korean Journal of Veterinary Service
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• v.46 no.1
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• pp.67-74
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• 2023

In the present study, the incidence of bovine tubeculosis (bTB) and brucellosis in Hanwoo (Korean native cattle) and dairy cow in Gyeongsangnam-do was investigated for three years from 2020 to 2022. The incidence bTB tested by tuberculin skin test with purified protein derivative (PPD) and gamma interferon (γ-INF) test with a commercial enzyme-linked immunosorbent assay. From 2020 to 2022, the incidence of bTB showed a decreasing trend in Hanwoo, while an increasing trend in dairy cow. In the case of Brucellosis, the positive rates for Hanwooe gradually increased. However, no brucellosis was found in dairy cow from 2020 to 2022. The increase in the incidence of these diseases is presumed to be related the small scale and poor sanitation facilities of livestock farms in Gyeongsangnam-do, and easy access of wild animals. Therefore, in order to suppress the incidence of the diseases, it is necessary to the farm scale from small to large and to strengthen sanitary facilities on farms.

• Weed & Turfgrass Science
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• v.7 no.3
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• pp.209-218
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• 2018

This study was carried out to investigate the occurrence and distribution of ALS inhibiting herbicide-resistant weeds and to estimate the appeared areas of resistant weeds in the paddy fields of Gyeongnam province of Korea in 2017 and 2018 using a soil assay method. Compared with the 2012 survey, this study showed that the infested ratio of ALS inhibiting herbicide-resistant weeds increased from 1.0% to 66.8% and the infested area increased from 876 ha to 49,008 ha. The infested area of ALS inhibiting herbicide-resistant weeds was estimated in Ulsan-si (8.4%), Hapcheon-gun (8.3%), Haman-gun (7.9%), Goseong-gun (7.9%), Hadong-gun (7.3%), Jinju-si (7.2%), Changnyeong-gun (7.0%), Gimhae-si (6.4%), Miryang-si (5.5%), Busan-si (4.9%), Uiryeong-gun (4.6%), Namhae-gun (4.3%), Geochang-gun (4.2%), Changwon-si (3.8%), Geoje-si (2.9%), Yangsan-si (1.8%), Sancheong-gun (0.9%) and Tongyeong-si (0.4%), and the herbicide resistant weeds was not occurred in Hamyang-gun. The most dominant ALS inhibiting herbicide-resistant weeds in paddy fields were Monochoria vaginalis, followed by Echinochloa oryzicola, Lindernia dubia, Scirpus juncoides, Ludwigia prostrata, Cyperus difformis, Sagittaria trifolia and Rotala indica. ALS inhibiting herbicide-resistant M. vaginalis, L. dubia, and E. oryzoides occurred throughout Gyeongnam province, and ALS inhibiting herbicide-resistant S. trifolia and R. indica were only found in Gimhae-si. Therefore, these results will be utilized to estimate population dynamics of ALS inhibiting herbicide-resistant weeds and provide proper management practices in the paddy fields of Gyeongnam province.

• Journal of Veterinary Clinics
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• v.27 no.3
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• pp.289-294
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• 2010

Mandarin fish (SSOGARI: Korean name) has emerged as new aquaculture and aquarium fish among Korean native fish species. Unfortunately, this fish could not exempt from infectious agent invasion. As an epidemiological survey, high mortality of mandarin fish (Siniperca scherzeri) observed from a fish farm in Gyeongnam province of South Korea. The major macroscopic sign of the diseased fish was severe multifocal dermal ulceration. Histological observation revealed inflammation, necrosis and colonization of bacteria in various tissues. Seven bacterial isolates from the diseased fish were identified as Aeromonas salmonicida subsp. salmonicida (typical A. salmonicida) using API 20E and 20NE system. However, the polymerase chain reaction (PCR) assay using two different primers (AP and MIY) revealed that the bacterial strains were not typical A. salmonicida, but atypical A. salmonicida. This study demonstrates that PCR assay is a reliable method for the confirmation of atypical A. salmonicida in mandarin fish as compared to biochemical tests. Furthermore, this is the first account of extensive dermatitis in mandarin fish due to atypical A. salmonicida infection, which has high potential in aquaculture and aquarium fish among native fish species.

• Journal of the Mineralogical Society of Korea
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• v.24 no.1
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• pp.19-29
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• 2011

Clay mineral assemblages of core PC25A collected from the northern part of the Aleutian Basin in the Bering Sea were examined in order to investigate changes in sediment provenances and transport pathways. Ages of core PC25A were determined by both Last Appearance Datum of radiolaria (L. nipponica sakaii; $48.6{\pm}2\; ka$) and age control points obtained by the correlations of $a^{\ast},\; b^{\ast}$, and laminated sediment layers with the adjacent core PC23A, whose ages are well constrained. The corebottom age of core PC25A was calculated to be about 57,600 yr ago and core-top might be missing during coring execution. Average contents of smectite, illite, kaolinite, and chlorite during the last glacial period are 11% (5~24%), 47% (36~58%), 13% (9~19%), and 29% (21~40%), respectively. Clay mineral assemblages of the last glacial period are characterized by higher illite and lower smectite contents than those of core MC24 representing the modern values. Illite-rich clay sediments during the warm Early Holocene were transported from the northern part of Alaska continent (Province 1) through the ice-melt waters. During the deglacial period (B${\phi}$lling-All${\phi}$rod) of MIS 2, clay-sized particles seemed to be also transported by ice-melt waters mainly from Province 2 and Province 3 located farther south than Province 1. Higher smectite content during the Last Glacial Maximum is attributed to increased amounts of clay particles from the adjacent Alaska Peninsula (Province 4). From the early to the middle MIS 3, illite and smectite contents decreased, whereas chlorite content increased. With the low sea level standing during MIS 3 the supply of clay sediments from Province 2 and Province 3 was most likely intensified. Changes in clay mineral assemblages of core PC25A located in the northern part of the Aleutian Basin in the Bering Sea are closely related to the change of surface current system caused by sea level variation during the last glacial period.