• Korean Journal of Weed Science
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• v.10 no.4
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• pp.261-268
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• 1990

Various herbicides were applied to the young rice seedling from which endosperm were removed and their effects were determined at 40 days after transplanting. Generally, growth was retarded, but the number of tillers was not effected. Application of bensulfuron and pretilachlor showed slight growth inhibition compared to dimepiperate and pyrazolate. Injury due to herbicide application was dependent on the transplanting depth. Injury was severe when pretilachlor and pyrazolate were applied to rice transplanted 0 and 4cm deep, respectively. However, bensulfuron showed much injury regardless of transplanting depths with the tendency of general growth retardation. Dimepiperate was fairly safe at all transplanting depths. The application of herbicides at 3 and 6 DAT showed higher injury than that at g and 12 DAT. The degree of injury was severe when pretilachlor and bensulfuron were applied.

• Research in Plant Disease
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• v.14 no.3
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• pp.159-164
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• 2008

Crown gall of rose (Rosa hybrida) was observed in greenhouse during 2003-2007. The average disease incidence was up to 38.1 % and was more severe in hydroponic culture as compared to soil culture. The typical gall symptom occurred mainly on the root, crown, or both, and resulted on poor rooting, growth retardation and yield loss. The reduction rate of rooting influenced by crown gall was 57.5% as compared to healthy plants on nursery stock. The location of gall formation in the plant influenced growth vigor resulting in symptoms such as poor shooting. Healthy plants produced 19.1 flowers/$m^2$, while diseased plants produced 9.5 flowers/$m^2$ during the same cultivation period. Moreover, the number of days to flowering was longer for the diseased plants than for healthy plants - 51.2 days and 39.8 days for first harvest, and 60.6 days and 52.1 days for the second harvest, respectively. Conclusively, infection on the basal stem caused serious loss of the number of shoot formation; yield loss of cut flower was 38.7% due to crown gall infection and delay of harvesting time about 8-10 days.

• Nutrition Research and Practice
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• v.11 no.2
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• pp.121-129
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• 2017

BACKGROUND/OBJECTIVES: The study was conducted to evaluate the effects of dietary leucine supplementation on mitochondrial biogenesis and energy metabolism in the liver of normal birth weight (NBW) and intrauterine growth-retarded (IUGR) weanling piglets. MATERIALS/METHODS: A total of sixteen pairs of NBW and IUGR piglets from sixteen sows were selected according to their birth weight. At postnatal day 14, all piglets were weaned and fed either a control diet or a leucine-supplemented diet for 21 d. Thereafter, a $2{\times}2$ factorial experimental design was used. Each treatment consisted of eight replications with one piglet per replication. RESULTS: Compared with NBW piglets, IUGR piglets had a decreased (P < 0.05) hepatic adenosine triphosphate (ATP) content. Also, IUGR piglets exhibited reductions (P < 0.05) in the activities of hepatic mitochondrial pyruvate dehydrogenase (PDH), citrate synthase (CS), ${\alpha}$-ketoglutarate dehydrogenase (${\alpha}$-KGDH), malate dehydrogenase (MDH), and complexes I and V, along with decreases (P < 0.05) in the concentration of mitochondrial DNA (mtDNA) and the protein expression of hepatic peroxisome proliferator-activated receptor-${\gamma}$ coactivator $1{\alpha}$ (PGC-$1{\alpha}$). Dietary leucine supplementation increased (P < 0.05) the content of ATP, and the activities of CS, ${\alpha}$-KGDH, MDH, and complex V in the liver of piglets. Furthermore, compared to those fed a control diet, piglets given a leucine-supplemented diet exhibited increases (P < 0.05) in the mtDNA content and in the mRNA expressions of sirtuin 1, PGC-$1{\alpha}$, nuclear respiratory factor 1, mitochondrial transcription factor A, and ATP synthase, $H^+$ transporting, mitochondrial F1 complex, ${\beta}$ polypeptide in liver. CONCLUSIONS: Dietary leucine supplementation may exert beneficial effects on mitochondrial biogenesis and energy metabolism in NBW and IUGR weanling piglets.

• Weed & Turfgrass Science
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• v.4 no.2
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• pp.118-123
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• 2015

Metagenomics is a powerful tool to isolate novel biocatalyst and biomolecules directly from the environmental DNA libraries. Since the metagenomics approach bypasses cultivation of microorganisms, un-cultured microorganisms that are majority of exists can be the richest reservoir for natural products discovery. To discover novel herbicidal substances from soil metagenome, we established three easy, simple and effective high throughput screening methods such as cucumber cotyledon leaf disc assay, microalgae assay and seed germination assay. Employing the methods, we isolated two active single clones (9-G1 and 9-G12) expressing herbicidal activity which whitened leaf discs, inhibited growth of microalgae and inhibited root growth of germinated Arabidopsis seeds. Spraying butanol fraction of the isolated active clones' culture broth led to growth retardation or desiccation of Digitalia sanguinalis (L) Scop. in vivo. These results represent that the screening methods established in this study are useful to screen herbicidal substances from metagenome libraries. Further identifying molecular structure of the herbicidal active substances and analyzing gene clusters encoding synthesis systems for the active substances are in progress.

• Horticultural Science & Technology
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• v.31 no.5
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• pp.538-543
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• 2013

During summer in Korea, the excessively high temperature causes growth retardation and quality reduction in cut roses grown in greenhouse. Mist treatments were conducted to reduce the temperature and avoid quality reduction of cut roses. The temperature change in the greenhouse, growth and quality of cut roses, and injuries caused by insects or fungi were investigated during mist treatment. Daily maximum temperature reduced as the number of mist treatment increased, resulting in $6^{\circ}C$ reduction by mist treatment for 10 seconds at 5 min interval. This temperature reduction occurred only when maximum temperature was over $40^{\circ}C$ in greenhouse, and not when it was less than $40^{\circ}C$ or rainy and/or cloudy day. Plant height and fresh weight of the cut roses were increased at the range of 10-20% by mist treatment. As frequency of mist treatment increased, however, malformed flowers increased and vase life of cut rose was largely shortened. The injuries by insects like as beet armyworm larvae and scale insect increased as well. In conclusion, it is recommended that mist treatment must be used when the daily maximum temperature in a green house is over $40^{\circ}C$ and forecasting for disease or insects should be conducted as well.

• Neonatal Medicine
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• v.25 no.4
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• pp.161-169
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• 2018

Purpose: We assessed the influence of antenatal corticosteroid (ACS) on the inhospital outcomes of intrauterine growth restriction (IUGR) infants. Methods: A retrospective study was conducted with singletons born at $23^{+0}$ to $33^{+6}weeks$ of gestation at Seoul National University Hospital from 2007 to 2014. We compared clinical outcomes between infants who received ACS 2 to 7 days before birth (complete ACS), at <2 or >7 days (incomplete ACS), and those who did not receive ACS in IUGR and AGA infants. Multivariate logistic regression using Firth's penalized likelihood was performed. Results: 304 neonates with 91 IUGR neonates were eligible. Among AGA neonates, mortality (adjusted odds ratio [aOR], 0.13; 95% confidence interval [CI], 0.02 to 0.78), hypotension within 7 postnatal days (aOR, 0.20; 95% CI, 0.06 to 0.64), and severe bronchopulmonary dysplasia (BPD) or death (aOR, 0.24; 95% CI, 0.07 to 0.77) were lower in complete ACS group after adjusting for pregnancy induced hypertension and uncontrolled preterm labor. Mortality (aOR, 0.18; 95% CI, 0.04 to 0.78), hypotension (aOR, 0.26; 95% CI, 0.09 to 0.70), and severe BPD or death (aOR, 0.33; 95% CI, 0.12 to 0.92) were also lower in the incomplete ACS group. Among IUGR infants, after adjusting for birth weight and 5-minute Apgar score, inhaled nitric oxide use within 14 postnatal days was lower in both complete ACS (aOR, 0.07; 95% CI, 0.01 to 0.67) and incomplete ACS (aOR, 0.04; 95% CI, 0.01 to 0.37) groups. Conclusion: ACS was not effective in reducing morbidities in IUGR preterm infants.

• IMMUNE NETWORK
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• v.4 no.1
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• pp.31-37
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• 2004

Background: 1-8D gene is a member of human 1-8 interferon inducible gene family and is shown to be overexpressed in fresh colon cancer tissues. Three peptides 1-6, 3-5 and 3-7 derived from 1-8D gene were shown to have immunogenicity against colon cancer. Methods: To study tumor immunotherapy of these peptides we established an adoptive transfer model. $D^{b-/-}{\times}{\beta}2$ microglobulin (${\beta}2m$) null mice transgenic for a chimeric HLA-A2.1/$D^b-{\beta}2m$ single chain (HHD mice) were immunized with irradiated peptide-loaded RMA-S/HHD/B7.1 transfectants. Spleens were removed after last immunization, and splenocytes were re-stimulated in vitro. Lymphocytes from vaccinated HHD mice were transferred together with IL-2 to the tumor bearing nude mice that were challenged S.C. with the HCT/HHD/B7 colon carcinoma cell line that was found to grow in these mice. Results: Peptide 3-5 was found to be highly effective in CTL activity. Adoptively transferred anti-peptide 3-5 cytolytic T lymphocytes caused significant retardation in tumor growth. Conclusion: This study shows that peptide 3-5 can be the most effective candidate for the vaccine of adoptive immunotherapy against colon cancer.

• Journal of the korean academy of Pediatric Dentistry
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• v.26 no.1
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• pp.151-156
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• 1999

Self-injurious behavior is defined as deliberate harm to one's own body without suicidal intent. It usually occurs as head banging or hitting, skin cutting, or finger biting and includes ocular, genital and oral self-mutilation. Lesch-Nyhan syndrome is a rare X-linked recessively inherited disorder, caused by complete absence of hypoxanthin-guanine phosphoribosyl transferase(HPRT) activity. Clinical presentation is characterized by mental retardation, chorea, athetosis, hyperuricemia, uricosuria and self-mutilating behavior. In these patients, the most typical feature is loss of tissue from biting themselves, even though they are not insensitive to pain. The dental management of self-mutilation includes treatment with appliances such as soft mouthguard or lip bumper, extraction of all the teeth, and orthognathic surgery. We report a 25-month-old boy who was a known case of Lesch-Nyhan syndrome and presented with severe self-mutilation wound on his lower lip. Vital pulpectomy and coronal resection was done as a more conservative approach than extracting all primary anterior teeth. Due to maintaining the root portion of the teeth in the bone, it is expected that the normal growth of the alveolar bone will be achieved.

• Journal of the Korean Society of Groundwater Environment
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• v.2 no.1
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• pp.22-29
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• 1995

Mobile bacterial particles can act as carriers and enhance the transport of hydrophobic contaminants in ground water by reducing retardation effects. Because of their colloidal size and favorable surface conditions, bacteria can act as efficient contaminant carriers. When such carriers exist in a porous medium, the system can be thought of as three phases: an aqueous phase, a carrier phase, and a stationary solid matrix phase. Contaminant can be present in either or all of these phases. In this study, a mathematical model based on mass balances is developed to describe the transport and fate of biodegradable contaminant in a porous medium. Bacterial mass transfer mechanism between aqueous and solid matrix phases, and contaminant mass transfer between aqueous and bacterial phases are represented by kinetic models. Governing equations are non-dimensionalized and solved to analyze the bacteria facilitated contaminant transport. The numerical results of the facilitation effect match favorably with experimental data reported in the literature. Results show that the contaminant transport can be described by local equilibrium assumption when Damkohler numbers are larger than 10. Significant sensitivities to model parameters, particularly bacterial growth rate and influent bacterial concentration, were discovered.

• Journal of the korean academy of Pediatric Dentistry
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• v.37 no.4
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• pp.545-550
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• 2010

Freeman-Sheldon Syndrome (FSS, also known as "Whistling Face Syndrome") is a rare genetic condition which characteristically includes a small "whistling" mouth, a flat mask-like face, club feet, joint contractures usually involving the fingers and hands, and under-development of the cartilage of the nose. Intelligence is usually normal. Most of the features of this syndrome are due to muscle weakness. The patient, 11 years old boy was consulted from pediatrics to pediatric dentistry due to dental management. After clinical & radiographic examinations, severe multiple problems were found. Dental problems were microsomia(whistling mouth) & micrognathia, perioral muscle contracture, restricted mouth opening, poor oral hygiene & care, generalized dental caries, high palatal vault, severe malocclusion & crowding. And Orthopedic problems, ophthalmic & respiratory, anesthetic problems were found. Then He also had psychiatric problem, hospital(dental) phobia due to previous medical history(frequent hospitalization). And he had genital problem, cryptochidism, too. Due to these intricate problems, he suffered with feeding, swallowing difficulties and showed growth retardation. For enhancing patient's oral health, pediatric dentist, orthodontist, oral surgeon, pediatrician, psychiatrist, orthopedist, they all agree with early, cautious intervention and treatment. So, he has been treated by multidisciplinary care, now he is recovering general health maintenance.