• Journal of Microbiology and Biotechnology
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• 제11권4호
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• pp.656-662
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• 2001

Angiogenesis is known as a crucial process in the growth and spreading of tumor cells. Accordingly, the effective inhibition of this process would appear to be a promising way to cure angiogenesis-related diseases, including cancer. This study demonstrates that acalycixenolide E (AX-E) from the marine organism Acalycigorgia inermis exhibits a potent anti-angiogenic activity both in vitro and in vivo. AX-E inhibits the bFGF-induced proliferation of HUVECs in a dose dependent manner, along with the bFGF-induced migration, invasion, and tube formation of HUVECs. Moreover, AX-E potently inhibits the in vivo neovascularization of the chorioallantoic membranes (CAMs) of growing chick embryos. interestingly, AX-E suppresses the expression of metalloproteases 2 and 9, yet shows no effect on their activities. The novel chemical structure and potent anti-angiogenic activity of AX-E will be of great value in elucidating the molecular mechanism of angiogenesis as well as in the development of a novel anti-angiogenic drug.

• 한국가금학회지
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• 제29권4호
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• pp.249-253
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• 2002

본 연구는 성장중인 닭에 있어서 질소 이용에 미치는 맹장절제 및 요소사료 첨가의 영향을 조사하기 위해서 실시되었다. 시험 사료는 19% 단백질 사료와 여기에 1% 요소를 첨가한 사료를, 시험 닭은 모의수술을 실시한 대조구와 맹장절제 수술을 실시한 처리구를 이용한 2 $\times$ 2의 요인시험법으로 행해졌다. 6일 동안의 본 시험기간 동안 물과 사료는 자유 섭취시켰다. 맹잘절제 수술닭의 사료효율은 요소의 급여에 의해서 유의하게 감소하였다(P<0.05). 19% 단백질사료 급여조건에서 맹장절제 수술 닭은 소화관내 사료의 통과시간을 유의하게 감소시켰지만(P<0.05), 반대로 19% 단백질사료 + 1% 요소 급여조건에서는 유의하게 증가시켰다(P<0.05). 맹장절제 수술은 사료에 관계없이 수분 배설량을 유의하게 증가시켰으며(P<0.05), 요소의 급여는 맹장절제 수술과 관계없이 수분의 배설량을 유의하게 증가시켰다(P<0.05). 양 사료 급여조건에서 맹장절제 수술은 요산 질소의 배설량을 체중 1009당 약 30 mg 정도씩 감소시켜서(P<0.05), 총 질소 배설에 대한 요산 질소의 배설비를 유의하기 감소시켰다(P<0.05). 결론적으로 성장중인 닭에서의 맹장절제 수술은 요산의 배설량을 감소시켜서 질소의 이용률을 개선 시켰으며, 본 연구의 결과는 이전에 성계를 통해서 얻어진 결과와도 일치하였다.

• 한국가금학회지
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• 제29권4호
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• pp.255-258
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• 2002

본 연구는 성장중인 닭에 있어서 맹장내 혐기성 미생물수와 맹장의 길이와의 관계를 구명하기 위해서 실시되었다. 5주령의 160수의 닭을 80수씩 분리하여 케이지내 사육 (대조구)및 염소 방목장내 염소와 같이 사육 (처리구)의 2처리로 구분하여 실시하였으며, 본 시험기간동안의 사료는 닭의 성장단계에 적합한 시판의 사료를 기초사료로 활용하였다. 시험기간 (5∼8주형)까지의 증체량은 8주령 에서는 대조구가 처리구보다 유의하게 놀았으며(P<0.05), 12주령에서는 처리구가 대조구보다도 유의하게 높았다(p<0.05). 맹장의 길이는 6, 9, 10 및 11주령에 처리구가 대조구보다 유의하게 길었다 (P<0.05). 맹장내 총 혐기성미생물수도 6, 9, 10 빛 11주령에 처리구가 대조구보다 유의하게 많았다(P<0.05). 대조구 및 처리구 함께 맹장의 길이와 증체량간에는 r= 0.816의 상관관계가 인정되었다. 맹장의 길이와 맹장내 혐기성 미생물 수는 대조구 및 처리구 함께 r=0.95이상의 높은 상관 관계가 인정되었다(P<0.01). 결론적으로 성장중인 닭에 있어서 섬유소 함량이 높은 염소의 배설물 섭취 등의 영향에 따른 맹장내 혐기성 미생물 수의 증가가 맹장의 길이 증가에 관계되어진다.

• Applied Biological Chemistry
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• 제46권1호
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• pp.50-54
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• 2003

호장근에서 분리한 emodin은 VEGF로 유도된 혈관신생의 한 단계인 혈관내피세포의 이동을 강하게 억제하였다. 또한 emodin은 혈관내피세포 이동을 억제시킨 $0.1\;{\mu}g/ml$ 농도에서 시험관내 혈관신생을 억제하였으며, 그 효과는 농도의존적인 양상을 보였다. 생체내 혈관신생 모델인 CAM assay에서도 emodin은 혈관신생을 억제하였다. 이러한 결과는 emodin이 현재까지 보고된 여러 가지 생리활성 이외에 혈관신생 억제활성을 가지고 있다는 사실을 보여주는 것이다. 따라서 emodin을 함유하고 있는 호장근은 혈관신생 관련 질환에 대한 천연물 유래 치료제의 개발을 위한 중요한 식물자원으로 활용될 수 있을 것이다.

• Asian-Australasian Journal of Animal Sciences
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• 제12권3호
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• pp.388-394
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• 1999

Two experiments were conducted to subdivide isoleucine (exp. 1) and valine (exp. 2) requirements for maintenance from the requirements for growth of broilers aged 1 to 3 weeks. Purified diets were used, containing five graded levels of isoleucine and valine. Based on weight gain response, the isoleucine requirement for growth was 7.50 mg/g weight gain and the daily isoleucine need for maintenance (mg) was 0.044 per unit metabolic body size ($(Wg^{0.75})$). Based on the N gain response, the isoleucine requirement for growth was 0.317 mg/mg N gain and the daily isoleucine need for maintenance (mg) was 0.040 per unit metabolic body size $(Wg^{0.75})$. Based on weight gain and N gain response, the total isoleucine requirement was calculated 244 mg/day or 0.59% of the diet, 274 mg/day or 0.66% of the diet, respectively. From the relationship of weight gain and N gain, 5.07% of the retained protein was comprised of isoleucine; the reported isoleucine content of chick muscle was 4.42%. The valine requirement for growth was 9.84 mg/g weight gain and 0.36 mg/mg N gain whereas the maintenance requirement was 0.046 or 0.052 mg per unit of metabolic body size (Wgo.11. According to the model developed to estimate valine requirement, the total requirement was 319 mg/day or 0.77% of the diet, 315 mg/day or 0.76% of the diet, respectively. Previous reported valine requirements for growing chicks of 7~24 days old were in close agreement with these estimates. As a percentage of retained protein, valine was calculated to be 5.81% ; the reported valine concentration of crude protein of chicks' body including feathers was 6.72%.

• Asian-Australasian Journal of Animal Sciences
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• 제12권3호
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• pp.381-387
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• 1999

Two experiments were conducted to subdivide threonine (exp. 1) and glycine (exp. 2) requirements of broilers into maintenance and growth requirements. Purified diets containing five graded levels of threonine (exp. 1) and glycine (exp. 2) were fed to growing chicks to estimate threonine (exp. 1) and glycine (exp. 2) requirements for growth and maintenance. A model developed to divide threonine requirement for maintenance from that for growth yielded a requirement for growth of 8.946 mg/g weight gain and 0.341 mg/mg N gain; the maintenance requirement was 0.033 or 0.030 mg per unit of metabolic body size $(Wg^{0.75})$. The plateau of plasma threonine concentration occurred at 279.4 mg threonine intake/day. The total threonine requirement was 289.1 mg/day or 0.69% of the diet, 294.1 mg/day or 0.71% of the diet based on weight gain and nitrogen gain responses, respectively. These estimates were in close agreement with previous estimates of threonine requirements. From the relationship of weight gain to N gain, 5.46% of the retained protein consisted of threonine; the reported threonine content of chick muscle was 4.02%. The glycine requirement for maintenance could not be determined due to failure to obtain data allowing extrapolation to zero response. However, ADG increased slightly up to 0.56% glycine.

• Molecules and Cells
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• 제38권3호
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• pp.273-278
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• 2015

The induction of angiogenesis is a crucial step in tumor progression, and therefore, efficient inhibition of angiogenesis is considered a powerful strategy for the treatment of cancer. In the present study, we report that the lipophilic antimicrobial peptides from EML-CAP3, a new endophytic bacterial strain isolated from red pepper leaf (Capsicum annuum L.), exhibit potent antiangiogenic activity both in vitro and in vivo. The newly obtained antimicrobial peptides effectively inhibited the proliferation of human umbilical vein endothelial cells at subtoxic doses. Furthermore, the peptides suppressed the in vitro characteristics of angiogenesis such as endothelial cell invasion and tube formation stimulated by vascular endothelial growth factor, as well as neovascularization of the chorioallantoic membrane of growing chick embryos in vivo without showing cytotoxicity. Notably, the angiostatic peptides blocked tumor cell-induced angiogenesis by suppressing the expression levels of hypoxia-inducible $factor-1{\alpha}$ and its target gene, vascular endothelial growth factor (VEGF). To our knowledge, our findings demonstrate for the first time that the antimicrobial peptides from EML-CAP3 possess antiangiogenic potential and may thus be used for the treatment of hypervascularized tumors.

• 대한본초학회지
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• 제26권3호
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• pp.83-90
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• 2011

Objectives : This study was performed to investigate the influence of black ginseng radix extracts (BG) and ginsenoside Rg3, Rg5 on basic fibroblast growth factor (bFGF) induced proliferation, migration and capillary tubule-like formation of human umbilical vein endothelial cells (HUVECs). Methods : HUVECs were cultured with BG and ginsenoside Rg3, Rg5 at different concentrations (60, 125, 250, 500, $1,000{\mu}g/m\ell$) for 2 day In the presence of bFGF, respectively. XTT was used to detect the proliferation. Migration and tube formations were examined to detect the antiangiogenesis. Also, the chick embryo chorioallantoic membrane (CAM) assay was performed to detect the antiangiogenesis. Results : BG and ginsenoside Rg3, Rg5 significantly inhibited bFGF-induced endothelial cell proliferation and migration in a dose-dependent manner. Tube formation in bFGF-induced HUVECs were suppressed by BG and ginsenoside Rg3, Rg5. Moreover, BG and ginsenoside Rg3, Rg5 (30-$50{\mu}g$/egg) inhibited new blood vessel formation on the growing CAM. Conclusions:Based on the present results, it can be suggested that BG has a potential chemopreventive agent via antiangiogenesis.

• The Korean Journal of Physiology and Pharmacology
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• 제12권5호
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• pp.267-274
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• 2008

Since first discovered in chick skeletal muscles, stretch-activated channels (SACs) have been proposed as a probable mechano-transducer of the mechanical stimulus at the cellular level. Channel properties have been studied in both the single-channel and the whole-cell level. There is growing evidence to indicate that major stretch-induced changes in electrical activity are mediated by activation of these channels. We aimed to investigate the mechanism of stretch-induced automaticity by exploiting a recent mathematical model of rat atrial myocytes which had been established to reproduce cellular activities such as the action potential, $Ca^{2+}$ transients, and contractile force. The incorporation of SACs into the mathematical model, based on experimental results, successfully reproduced the repetitive firing of spontaneous action potentials by stretch. The induced automaticity was composed of two phases. The early phase was driven by increased background conductance of voltage-gated $Na^+$ channel, whereas the later phase was driven by the reverse-mode operation of $Na^+/Ca^{2+}$ exchange current secondary to the accumulation of $Na^+$ and $Ca^{2+}$ through SACs. These results of simulation successfully demonstrate how the SACs can induce automaticity in a single atrial myocyte which may act as a focus to initiate and maintain atrial fibrillation in concert with other arrhythmogenic changes in the heart.

• Animal Bioscience
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• 제34권11호
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• pp.1811-1821
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• 2021

Objective: A trial was conducted to investigate the effects of supplemental levels of Mn provided by organic and inorganic trace mineral supplements on growth, tissue mineralization, mineral balance, and antioxidant status of growing broiler chicks. Methods: A total of 500 male chicks (8-d-old) were used in 10-day feeding trial, with 10 treatments and 10 replicates of 5 chicks per treatment. A 2×5 factorial design was used where supplemental Mn levels (0, 25, 50, 75, and 100 mg Mn/kg diet) were provided as MnSO₄·H₂O or MnPro. When Mn was supplied as MnPro, supplements of zinc, copper, iron, and selenium were supplied as organic minerals, whereas in MnSO₄·H₂O supplemented diets, inorganic salts were used as sources of other trace minerals. Performance data were fitted to a linearbroken line regression model to estimate the optimal supplemental Mn levels. Results: Manganese supplementation improved body weight, average daily gain (ADG) and feed conversion ratio (FCR) compared with chicks fed diets not supplemented with Mn. Manganese in liver, breast muscle, and tibia were greatest at 50, 75, and 100 mg supplemental Mn/kg diet, respectively. Higher activities of glutathione peroxidase and superoxide dismutase (total-SOD) were found in both liver and breast muscle of chicks fed diets supplemented with inorganic minerals. In chicks fed MnSO₄·H₂O, ADG, FCR, Mn balance, and concentration in liver were optimized at 59.8, 74.3, 20.6, and 43.1 mg supplemental Mn/kg diet, respectively. In MnPro fed chicks, ADG, FCR, Mn balance, and concentration in liver and breast were optimized at 20.6, 38.0, 16.6, 33.5, and 62.3 mg supplemental Mn/kg, respectively. Conclusion: Lower levels of organic Mn were required by growing chicks for performance optimization compared to inorganic Mn. Based on the FCR, the ideal supplemental levels of organic and inorganic Mn in chick feeds were 38.0 and 74.3 mg Mn/kg diet, respectively.