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Bioactive Characterization of Bacillus thuriniensis subsp. kurstaki CAB133 Isolated from Domestic Soil (국내 토양으로 분리된 Bacillus thuriniensis subsp. kurstaki CAB133균주의 생물학적 특성)

  • Choi, Su-Yeon;Cho, Min-Su;Kim, Tae-Hwan;Kim, Jin-Su;Pack, Seung-Kyung;Youn, Young-Nam;Hong, Soon-Sung;Yu, Yong-Man
    • Korean journal of applied entomology
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    • v.47 no.2
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    • pp.175-184
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    • 2008
  • To screen highly active Bacillus thuringiensis isolates against Spodoptera litura (Lepidoptera, Noctuidae), 46 B. thuringiensis was isolated from 115 samples obtained from several crop soils. Especially, B. thuringiensis subsp. kurstaki CAB133 and CAB162 isolates showed 100% mortality against S. litura. $LD_{50}$ values of CAB 133, CAB162 and HD-1 strains of B. thuringiensis subsp. kurstaki were 0.089, 3.144 and $0.513{\mu}g/ml$ against 2nd larva of S. litura, respectively. The weight of 3rd larva of S. litura which were fed crystal inclusion protein $(1.267{\mu}g/ml)$ with B. thuringiensis subsp. kurstaki CAB133 was about 30 times lass than control group. CAB133 and CAB 162 strains of B. thuringiensis subsp. kurstaki which were taken a highly toxity against S. litura were analyzed by SDS-PAGE, and estimated the molecular weight of the Cry proteins. Their serological identification by H serotypes were showed B. thuringiensis subsp. kurstaki (3abc) type.

Field Survey of Greenhouse for Strawberry Culture -Case Study Based on Western Gyeongnam Area- (딸기재배 온실의 현장조사 분석 -서부경남 지역을 중심으로-)

  • Jeong, Young Kyun;Lee, Jong Goo;Yun, Sung Wook;Kim, Hyeon Tae;Yoon, Yong Cheol
    • Journal of Bio-Environment Control
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    • v.27 no.3
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    • pp.253-259
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    • 2018
  • This study set out to select a system to realize an optimal environment for strawberry cultivation greenhouses based on data about the growth and development of strawberry and its environment and to provide basic data for the research of its improved productivity. For these purposes, the investigator conducted a field survey with greenhouses for strawberry cultivation in western Gyeongnam. The findings show that farmers in their fifties and sixties accounted for the biggest part in the age groups of strawberry farmers. While those who were under 50 were accounted for approximately 67.5%, those who were 60 or older accounted for 32.5%. As for cultivation experiences, the majority of the farmers had ten years of cultivation experiences or less with some having 30 years of cultivation experiences or more. All the farmers built an arch type single span greenhouse. Those who used nutrient solutions were about 75.0%, being more than those who used soil. All of the farmers that used a nutrient solution adopted an elevated hydroponic system. The single span greenhouses were in the range of 7.5~8.5m, 1.3~1.8m and 2.5~3.5m for width, eaves, and ridge height, respectively, regardless of survey areas. The rafters interval was about 0.7~0.8m. In elevated hydroponic cultivation, the width, height, and interval of the beds were about 0.25m, 1.2m and 1.0m, respectively. As for the strawberry varieties, the domestic ones accounted for approximately 97.5% with Seolhyang being the most favorite one at about 65.0%. As for the internal environment factors of greenhouses, 38 farmers measured only temperature and relatively humidity. As for hydroponics, the farmers used a hydroponics control system. Except for the farmers that introduced a smart farm system for temperature and humidity control, approximately 85.0% controlled temperature and humidity only with a control panel for side windows and ventilation fans. As for heating and heat insulation, all of the farmers were using water curtains with many farmers using an oil or electric boiler, radiating lamp or non-woven fabric, as well, when necessary.

Magnetic Resonance Imaging of Breast Cancer Patients with BRCA Mutation (BRCA 유전자 변이가 있는 유방암 환자의 자기공명영상)

  • Chung, Sun Young;Cha, Joo Hee;Kim, Hak Hee;Shin, Hee Jung;Kim, Hyun Ji;Chae, Eun Young;Shin, Ji Eun;Choi, Woo Jung;Hong, Min Ji;Ahn, Sei Hyun;Lee, Jong Won;Jung, Kyung Hae
    • Investigative Magnetic Resonance Imaging
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    • v.17 no.3
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    • pp.207-214
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    • 2013
  • Purpose : To evaluate the MRI findings of breast cancer with BRCA mutation. Materials and Methods: We collected information of the breast cancer patients who underwent the test for BRCA gene mutation as well as preoperative breast MRI from January 2007 to December 2010. A total of 185 patients were enrolled; 33 of these patients had BRCA mutations and 152 patients did not. Among them, a total of 231 breast cancers were detected. Images of the 47 breast cancers with BRCA mutation and of the 184 breast cancers without mutations were evaluated to compare the morphologic and enhancement features on MRI. Results: With MR imaging, there were no significant difference in morphologic characteristic between two groups. However, enhancement pattern in the group with BRCA mutation were more likely to have persistent enhancement (p < 0.233), and LN metastasis was more common in breast cancers without BRCA mutation. Breast cancers with BRCA 2 mutation tend to show more persistent enhancement pattern than BRCA 1 mutation. Conclusion: In breast cancer patients with BRCA mutation, MRI didn't show significant difference in morphologic characteristics, however breast cancers with BRCA gene mutation carriers tend to have benign morphologic features on MRI, such as Type 1 kinetic curve enhancement.

Karyotype Studies on Three Species of the Family Muridae (Mammalia; Rodentia) in Korea (한국산 쥐과 3종의 핵형에 관한 연구)

  • Kang, Yung-Sun;Koh, Hung-Sun
    • The Korean Journal of Zoology
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    • v.19 no.3
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    • pp.101-112
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    • 1976
  • 1. It has been found in the karyotype of Apodemus agrarius coreae that No. 1 chromosome pair is subtelocentric and this is the new chromosome type in comparison with acro-telocentric No. 1 pair of the other subpecies. 2. It was reported in the Karyotype of Microtus fortis from USSR that the autosome consisted of 2 submetacentric, 10 metacentric and 38 acrocentric chromosomes, and that X is acrocentric and Y is small acrocentric one. In the present study, however, the autosome of M. fortis pelliceus in Korea is composed of three groups; 4 subtelocentric, 10 meta-submetacenric, and 36 acrocentric one. And X is the largest metacentric chromosome of the complement. Y is smaller acrocentric one. Thus, it has been found that the karyotype of M. fortis in Korea differs from that of the same species in USSR. In the karyotype of this red vole, two pairs of heteromorohic chromosome with respect to the size of their secondary constrictions have been shown in the acrocentric group. 3. The diploid number of Cricetulus triton nestor was found to be 28, and its chromosome size ranges from 7.5 $\\mu$ to 1.5 $\\mu$. Autosomes contains 11 large acrocentric pairs and two pairs of very small metacentric ones. This feature is simillar to that of Tscherskia triton found USSR.

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Micro-tensile Bond Strength of Composite Resin Bonded to Er:YAG Laser-prepared Dentin (Er:YAG 레이저로 삭제된 상아질에 대한 컴포지트 레진의 미세인장결합강도에 관한 연구)

  • Min, Suk-Jin;Ahn, Yong-Woo;Ko, Myung-Yun;Park, June-Sang
    • Journal of Oral Medicine and Pain
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    • v.31 no.3
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    • pp.211-221
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    • 2006
  • Purpose The aims of this study were to evaluate micro-tensile bond strength of composite resin bonded to dentin following high-speed rotary handpiece preparation or Er:YAG laser preparation with two different adhesive systems and to assess the influence of different Er:YAG laser energies on the micro-tensile bond strength. Materials and Methods In this study, 40 third morlars were used. Flat dentin specimans were obtained and randomly assigned to eight groups. Dentin surfaces were prepared with one of four cutting types: carbide bur, Er:YAG laser (2 W, 3 W and 4 W) and conditioned with two bonding systems, Scotchbond Multipurpose Plus (SM), Clearfil SE bond (SE) and composite resin-build ups were created. After storage for 24 hours, each specimen was serially sectioned perpendicular to the bonded surface to produce more than thirty slabs in each group. Micro-tensile bond strength test was performed at a crosshead speed of 1.0 mm/min. Micro-tensile bond strengths (${\mu}TBS$) were expressed as means$\pm$SD. Data were submitted to statistical analysis using two-way ANOVA, one-way ANOVA, Student-Newman-Keuls' multiple comparison test and t-test. Results and Conclusion 1. Regardless of bonding systems, the ${\mu}TBS$ according to cutting types were from highest to lowest : 3 W, 2 W, Bur, and 4 W. In addition, there was no significant difference between Bur and 4 W (p<0.001). 2. Regardless of cutting types, SM showed significantly higher ${\mu}TBS$ than SE (p<0.001). 3. Bonding to dentin conditioned with SM resulted in higher ${\mu}TBS$ for 3 W compared to Bur, 2 W, and 4 W. There was no significant difference between 2 W and Bur (p<0.001). 4. Bonding to dentin conditioned with SE resulted in higher ${\mu}TBS$ for 3 W compared to 2 W, 4 W, and Bur. Bur exhibited significant lower ${\mu}TBS$ than all other cutting types. There were no significant differences between 3 W, 2 W and between 4 W and Bur (p<0.001). 5. The ${\mu}TBS$ of laser cutting groups were shown in order from highest to lowest: 3 W, 2 W and 4 W in two bonding systems. There was no significant difference between 2 W and 3 W in SE (p<0.001). : The ${\mu}TBS$ of composite resin bonded dentin was significantly affected by interaction between the cutting type and bonding system. In the range of 2 W-3 W, cavity preparation of the Er:YAG laser seems to supply good adhesion of composite resin restoration no less than bur preparation. In particular, if you want to use the self-etching system, including Clearfil SE bond for the purpose of a simplification of the bonding procedures and prevention of adverse effects by excessive etching, an Er:YAG laser may offer better adhesion than a bur.

Analysis of Epidemiological Characteristics, PFGE Typing and Antibiotic Resistance of Pathogenic Escherichia coli Strains Isolated from Gyeonggi-do (경기도에서 분리한 병원성대장균의 역학적 특성 및 PFGE, 항생제 내성 연구)

  • Kim, Kyung-A;Yong, Kum-Chan;Jeong, Jin-A;Huh, Jeong-Weon;Hur, Eun-Seon;Park, Sung-Hee;Choi, Yun-Sook;Yoon, Mi-Hye;Lee, Jong-Bok
    • Korean Journal of Microbiology
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    • v.50 no.4
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    • pp.285-295
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    • 2014
  • This study was conducted to survey the epidemiological characteristics and the isolated strains for pathogenic E. coli which was the major causative organisms for food poisoning occurred at school food services in the Gyeonggi-do area during the past three years. We investigated 19 accidents of food-borne disease outbreaks by pathogenic E. coli at school food services from 2010 to 2012. Food-borne disease outbreaks by pathogenic E. coli were usually occurred at direct management type (18 accidents, 95%) and high schools. For the seasonal factors, 13 accidents (65%) were occurred in June to September, especially the end of August and September after the summer holidays. The first patients were occurred on Wednesday (7 accidents, 37%) and Thursday (7 accidents, 37%), and they were mainly reported on Thursday (7 accidents, 37%) and Friday (5 accidents, 26%). The exposure of risk was estimated in Monday (4 accidents, 21%), Tuesday (7 accidents, 37%) and Wednesday (4 accidents, 21%), and kimchi (5 accidents, 50%) was estimated as the food of the high risk responsible for the outbreaks. 98 isolates of pathogenic E. coli consisted of PEC (50%), ETEC (34%), EAEC (15%), and EHEC (1%). The antibiotic resistance of pathogenic E. coli showed in the descending order of ampicilline (40%), nalidixic acid (37%), trimethoprim/sulfamethoxazole (24%), and tetracycline (19%). The antibiotics of second and third generation cephalosporins, cabarpenem, aminoglycosides, and second generation quinolones had antimicrobial susceptibilities and cefalotin, ampicillin/sulbactam and chloramphenicol showed medium resistance at 29%, 25%, and 6% respectively, and 70% of isolates were resistant to more than one antibiotic. By the PFGE analysis, they were classified into nine major groups and 31 profiles with 57% pattern similarity. It was very difficult to find the correlation of antimicrobial susceptibilities and genotype in the small scale-food poisoning, but the similarity of antimicrobial resistance and PFGE patterns in the large scale-food poisoning enabled the outbreaks to estimate the same pathotype of E. coli derived from identical origins.

Factors Predicting the Development of Radiation Pneumonitis in the Patients Receiving Radiation Therapy for Lung Cancer (방사선 치료를 시행 받은 폐암 환자에서 방사선 폐렴의 발생에 관한 예측 인자)

  • An, Jin Yong;Lee, Yun Sun;Kwon, Sun Jung;Park, Hee Sun;Jung, Sung Soo;Kim, Jin whan;Kim, Ju Ock;Jo, Moon Jun;Kim, Sun Young
    • Tuberculosis and Respiratory Diseases
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    • v.56 no.1
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    • pp.40-50
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    • 2004
  • Background : Radiation pneumonitis(RP) is the major serious complication of thoracic irradiation treatment. In this study, we attempted to retrospectively evaluate the long-term prognosis of patients who experienced acute RP and to identify factor that might allow prediction of RP. Methods : Of the 114 lung cancer patients who underwent thoracic radiotherapy between December 2000 and December 2002, We performed analysis using a database of 90 patients who were capable of being evaluated. Results : Of the 44 patients(48.9%) who experienced clinical RP in this study, the RP was mild in 33(36.6%) and severe in 11(12.3%). All of severe RP were treated with corticosteroids. The median starting corticosteroids dose was 34 mg(30~40) and median treatment duration was 68 days(8~97). The median survival time of the 11 patients who experienced severe RP was significantly poorer than the mild RP group. (p=0.046) The higher total radiation dose(${\geq}60Gy$) was significantly associated with developing in RP.(p=0.001) The incidence of RP did not correlate with any of the ECOG performance, pulmonary function test, age, cell type, history of smoking, radiotherapy combined with chemotherapy, once-daily radiotherapy dose fraction. Also, serum albumin level, uric acid level at onset of RP did not influence the risk of severe RP in our study. Conclusion : Only the higher total radiation dose(${\geq}60Gy$) was a significant risk factor predictive of RP. Also severe RP was an adverse prognostic factor.

COLOR STABILITY OF THE RESIN CEMENTS WITH ACCELERATED AGING (레진시멘트의 색안정성에 대한 가속시험)

  • Song, Ha-Jeung;Park, Su-Jung;Hwang, Yun-Chan;Oh, Won-Mann;Hwang, In-Nam
    • Restorative Dentistry and Endodontics
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    • v.33 no.4
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    • pp.389-396
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    • 2008
  • The purpose of this study was to evaluate the color stability of resin cements with accelerated test. Four dual curing resin cements: Panavia-F (KURARAY). Duolink (BISCO), Variolink-II (Ivoclar Vivadent), and RelyX Unicem (3M ESPE) and 1 self curing resin cement: Resiment CE (j. l. Blosser) were used in this study. In control group, Gradia Anterior (GC) composite resin and Tescera Dentin (Bisco) indirect composite were used. Ten disk shape specimens were made from each resin cement. The specimens were subjected to an accelerated aging process in a refrigerated bath circulator at 60$^{\circ}C$ for 15 and 30 days. Spectrophotometric analyses were made before and after 15 days and 30 days of accelerated aging time. The color characteristics ($L^*,\;a^*,\;b^*$) and the color difference (${\Delta}E^*$) of the specimens before and after immersion were measured and computed. Regardless of type of the resin cements, $L^*$ value was decreased and $a^*$ value was increased, but there were no significant difference. But $b^*$ value was increased significantly (p < 0.05). Tescera inlay showed least color change (p < 0.05), but Gradia showed notable color change after 15 days. After 30 days on accelerated aging, ${\Delta}E^*$ value was increased (Panavia-F < Variolink-II < Resiment CE < Duolink < Unicem) (p < 0.05). but there were no significant difference among Panavia-F, Variolink-II, and Resiment CE groups. After 30 days of accelerated aging, ${\Delta}E^*$ value of all resin cements were greater than 3.0 and could be perceived by the human eye.

A Study on the Arrangement and Design of Cultural Property Signs - Focused on the Seoul Tangible Cultural Properties Located in Jongno-gu Area - (문화재 안내판의 배치와 디자인에 대한 고찰 - 종로구 소재 서울 유형문화재를 중심으로 -)

  • So, Hyun-Su;Kim, Hai-Gyoung
    • Journal of the Korean Institute of Traditional Landscape Architecture
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    • v.30 no.1
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    • pp.66-75
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    • 2012
  • The Seoul Metropolitan Government selected the sign designs of cultural heritages through a public prize contest from a public design perspective and applied the selected design format to the signs of the cultural assets that were designated by the central and Seoul governments and located in Seoul Metropolitan area in 2008. For the purpose of monitoring the result, this study analyzed the installation state of the signs of Seoul tangible cultural properties located in Jongno-gu. The scope of the analysis of this study was expanded to cover the surrounding areas of cultural heritages in order to review the arrangement and design of such signs. The result of this study can be summarized as follows; firstly, not only the flow of travelers' movement but also the direction of sight, their locations in relation to the surrounding facilities or other installations and the integration with similar signs or notices were necessary to be considered in the arrangement of the signs; secondly, the current sings had low quality in terms of durability and readability and seemed utterly distance from the cultural assets indicated by the signs because they were finished with tempered glass; thirdly, the size, shape and materials of the signs were not harmonized with their surrounding areas as only one design was used in the entire cultural property sites without considering such natural or artificial backgrounds of individual signs as rocks, plants, walls or buildings. When selecting the design format of the signs of individual cultural properties that are located dispersively as Seoul tangible cultural properties, it is recommended to determine a group of most representative designs based on natural, man-made and cultural landscape rather than one unified design format and to use a unique well-matched sign for each cultural property. For this reason, this study selected alternative exemplary design models and proposed the type of signs appropriate for each cultural site.

Structural Behavior of the Buried flexible Conduits in Coastal Roads Under the Live Load (활하중이 작용하는 해안도로 하부 연성지중구조물의 거동 분석)

  • Cho, Sung-Min;Chang, Yong-Chai
    • Journal of Navigation and Port Research
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    • v.26 no.3
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    • pp.323-328
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    • 2002
  • Soil-steel structures have been used for the underpass, or drainage systems in the road embankment. This type of structures sustain external load using the correlations with the steel wall and engineered backfill materials. Buried flexible conduits made of corrugated steel plates for the coastal road was tested under vehicle loading to investigate the effects of live load. Testing conduits was a circular structure with a diameter of 6.25m. Live-load tests were conducted on two sections, one of which an attempt was made to reinforce the soil cover with the two layers of geo-gird. Hoop fiber strains of corrugated plate, normal earth pressures exerted outside the structure, and deformations of structure were instrumented during the tests. This paper describes the measured static and dynamic load responses of structure. Wall thrust by vehicle loads increased mainly at the crown and shoulder part of the conduit. However additional bending moment by vehicle loads was neglectable. The effectiveness of geogrid-reinforced soil cover on reducing hoop thrust is also discussed based on the measurements in two sections of the structure. The maximum thrusts at the section with geogrid-reinforced soil cover was 85-92% of those with un-reinforced soil cover in the static load tests of the circular structure; this confirms the beneficial effect of soil cover reinforcement on reducing the hoop thrust. However, it was revealed that the two layers of geogrid had no effect on reducing the overburden pressure at the crown level of structure. The obtained values of DLA decrease approximately in proportion to the increase in soil cover from 0.9m to 1.5m. These values are about 1.2-1.4 times higher than those specified in CHBDC.