• Toxicological Research
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• 제25권3호
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• pp.132-139
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• 2009

The toxicity test of ammonium persulfate was conducted to ensure of its potential toxic effects according to the single-dose acute oral toxicity study (OECD Guideline 423) and 90-day repeated dose sub-chronic oral toxicity study guideline (OECD Guideline 408) for establishing national chemical management system, and matching in the Globally Harmonized Classification System (GHS) category. In acute oral toxicity study, pasty stool, perineal contamination and temporary body weight decrease were observed after dosing 1st and 2nd challenge (300 mg/kg body weight). All test animals were dead within 6 hours after dosing at 3rd challenge (2000 mg/kg body weight). Therefore, the GHS class of test substance is considered class 4. In sub-chronic toxicity study, body weight changes, food consumptions, hematological, biochemical and pathological examination did not show any noticeable and significant differences between the administered (5, 20, 80 mg/kg body weight) and control (vehicle only) group animals. Based on these results, the no observed adverse effect level (NOAEL) is considered above 80 mg/kg body weight.

• 한국융합학회논문지
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• 제6권4호
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• pp.15-23
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• 2015

이 연구의 목적은 12주간 휠체어테니스 융합 교실에 참여하는 지체장애인의 기본적 심리욕구와 운동정서 및 운동 몰입에 어떠한 영향을 미치는지를 규명하는데 있다. 연구결과, 첫째, 기본적 심리욕구에서는 하위요인 중 자율성과 관계성에서 집단 간 차이가 나타났고 자율성, 유능성, 관계성의 측정시점에서도 차이가 나타났다. 둘째, 운동 몰입에서는 대한 하위요인 중 인지몰입과 행위몰입의 측정시점에서 차이가 나타났으며, 두 가지 하위요인의 집단과 측정시점에서도 상호효과가 나타났다. 셋째, 운동정서에서는 대한 하위요인인 긍정정서와 부정정서에서 집단 간과 측정시점에서 차이가 나타났으며, 두 가지 하위요인의 집단과 측정시점에서도 상호효과가 나타났다. 후속연구에서는 장애인들의 주관적인 경험과 더불어 어려운 점을 이끌어내기 위한 질적 연구가 병행됨으로써 보다 적극적인 장애인들의 지속적인 참여를 유도할 수 있을 뿐만 아니라, 다양한 프로그램의 개발이 가능할 것으로 생각된다.

• Journal of Korean Neurosurgical Society
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• 제51권3호
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• pp.127-134
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• 2012

Objective: To analyze the prognostic factors thought to be related with survival time after a spinal metastasis operation. Methods: We retrospectively analyzed 217 patients who underwent spinal metastasis operations in our hospital from 2001 to 2009. Hematological malignancies, such as multiple myeloma and lymphoma, were excluded. The factors thought to be related with postoperative survival time were gender, age (below 55, above 56), primary tumor growth rate (slow, moderate, rapid group), spinal location (cervical, thoracic, and lumbo-sacral spine), the timing of radiation therapy (preoperative, postoperative, no radiation), operation type (decompressive laminectomy with or without posterior fixation, corpectomy with anterior fusion, corpectomy with posterior fixation), preoperative systemic condition (below 5 points, above 6 points classified by Tomita scoring), pre- and postoperative ambulatory function (ambulatory, non-ambulatory), number of spinal metastases (single, multiple), time to spinal metastasis from the primary cancer diagnosis (below 21 months, above 22 months), and postoperative complication. Results: The study cohort mean age at the time of surgery was 55.5 years. The median survival time after spinal operation and spinal metastasis diagnosis were 6.0 and 9.0 months. In univariate analysis, factors such as gender, primary tumor growth rate, preoperative systemic condition, and preoperative and postoperative ambulatory status were shown to be related to postoperative survival. In multivariate analysis, statistically significant factors were preoperative systemic condition (p=0.048) and postoperative ambulatory status (p<0.001). The other factors had no statistical significance. Conclusion: The factors predictive for postoperative survival time should be considered in the surgery of spinal metastasis patients.

• 한국기술혁신학회:학술대회논문집
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• 한국기술혁신학회 2003년도 춘계학술대회
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• pp.413-425
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• 2003

Information Technology is the kernel technology deciding the industrial standard of one nation, and biotechnology will be the main technology of next generation. Based on this fact, a lot of efforts were made to industrialize them. Nano Technology is beginning to position itself as the kernel fusion technology, and its usage and popularity is expanding. Environmental and Energy Technology is a must-have strategic technology considering the increase demand of new energy development, the international environment correspondence, the environment-friendly production, and so forth. Space Technology is the field, which will contribute to raise the domestic component and system technology to the next level. In 2001, new technology research development costs total of 1 trillion 32 billion won in the following fields; 437.82 billion won in IT, 88.457 billion won in BT, 46.799 billion won in NT, 315.682 billion won in ET, and 112 billion won in ST. from component ratio, IT forms 42% which is the most, 31% for ET and in order of BT, ST and NT. ETRI and KISTI are concentrating on IT, KIBB is on BT, KAERI, KIER, KERI and KBSI are focusing on ET, and KIMM, KRISS, KRICT and KORDI is participating together in 4∼5 new technology such as IT, BT, NT and ET. Funds for research development costs in 5 new technology fields of 13 contribution (year) are consisted as follows; The Office for Government Policy Coordination has contributed 131 billion won (13%), 387 billion won (37%) by MOST, 256 billion won (25%) by Ministry of Information and Communication, 67 billion won (6%) by Ministry of Commerce, Industry and Energy, 19% by others and the industrial world. < Strategy for Technology Advancing > o Promotion of comprehensive contributing (year) new technology development research plan project o Increase research efficiency by promoting new technology development project connected with peculiar projects of organization by contribution (year) o Formation of superior research group by technology and introduction of operation system for research accumulation are needed. o Technology demand-oriented assignment deduction and promotion of research development project connected with intermediate long term objective o National will and investment extension of research development costs, training and popularization of professionals, commercialization promotion with efficient control for research plan and result.

• Asian Pacific Journal of Cancer Prevention
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• 제14권12호
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• pp.7523-7527
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• 2013

Thymidylate synthase (TS) catalyzes the transfer of a methyl group from methylenetetrahydrofolate to dUMP to form dTMP. It is a primary target in the chemotherapy of colorectal cancers and some other neoplasms. In order to obtain pure protein for analysis of structure and biological function, an expression vector TS-pET28b (+) was constructed by inserting wild-type human thymidylate synthase (hTS) cDNA into pET28b (+). Then an expression strain was selected after transformation of the recombined plasmid into Rosetta (DE3). Fusion protein with His-tag was efficiently expressed in the form of inclusion bodies after IPTG induction and the content was approximately 40.0% of total bacteria proteins after optimizing expression conditions. When inclusion bodies were washed, dissolved and purified by Ni-NTA under denatured conditions, the purity was up to 90%. On SDS-PAGE and West-blotting, the protein band was found to match well with the predicted relative molecular mass-36kDa. Bioactivity was 0.1 U/mg. The results indicated that high-level expression of wild-type hTS cDNA can be achieved in prokaryotes with our novel method, facilitating research into related chemotherapy.

• 한국분말재료학회지
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• 제22권3호
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• pp.181-186
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• 2015

In this study, the effect of the friction stir welding (FSW) was compared with that of the gas tungsten arc welding (GTAW) on the microstructure and microhardness of Cu-Ni alloy weldment. The weldment of 10 mm thickness was fabricated by FSW and GTAW, respectively. Both weldments were compared with each other by optical microstructure, microhardness test and grain size measurement. Results of this study suggest that the microhardness decreased from the base metal (BM) to the heat affected zone (HAZ) and increased at fusion zone (FZ) of GTAW and stir zone (SZ) of FSW. the minimum Hv value of both weldment was obtained at HAZ, respectively, which represents the softening zone, whereas Hv value of FSW weldment was little higher than that of GTAW weldment. These phenomena can be explained by the grain size difference between HAZs of each weldment. Grain size was increased at the HAZ during FSW and GTAW. Because FSW is a solid-state joining process obtaining the lower heat-input generated by rotating shoulder than heat generated in the arc of GTAW.

• 한국안광학회지
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• 제15권4호
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• pp.373-379
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• 2010

목적: 이 연구의 목적은 MYSTERY CIRCLE 시스템을 이용하여 폭주부족 사위와 간헐성 사시 환자의 시기능 훈련효과를 조사하였다. 방법: 시기능 훈련 대상자는 안질환과 조절이상 및 수직사위가 없는 폭주부족 사위(n=18)와 간헐성 사시(n=8) 이상자 26명을 대상으로 추적 관찰하였고, 훈련기간은 8주간 매주 한번은 안경원에 방문하여 시기능 훈련에 따른 변화를 측정하였다. 결과: MYSTERY CIRCLE 시스템으로 시기능 훈련을 실행한 결과 기능적 및 감각적 증상이 개선되었다. 그리고 Worth 4 Dot검사, 입체시 검사 및 적색렌즈 융합 속도 검사도 개선을 보여 주었다. 결론: MISTERY CIRCLE 시기능 훈련 시스템을 이용한 시기능 훈련은 좋은 양안시 이상 개선 효과를 보여주었다.

• Elastomers and Composites
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• 제34권3호
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• pp.229-238
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• 1999

본 연구에서는 제3의 성분으로 벤젠 술폰산계열 반응성 화합물과 ENB 및 DCPD를 사용하여 비diene계 고분자인 EVA를 특수 화학적 처리에 의하여 EVA의 주쇄에 불포화기 즉, 이중결합을 도입시켰다. 또한 이중결합도입 EVA와 diene계 범용합성고무인 CR, SBR과의 블렌드를 통하여 황가 황체를 제조하여 가황체들의 물리적 특성을 비교 검토하였다. 검토결과 원래의 EVA가 갖고 있는 green strength, 사출 압출 성형성, 접착성, 점착성, 치수안정성, 오존저항성 등의 장점은 그대로 살리면서도 황가황이 가능하여 열가소성의 EVA가 갖는 사용온도의 한계 문제와 기계적 특성 즉, 경도, 인장강도, 인장 응력, 신장율 등온 향상시켰다. 비diene계 고분자와 diene계 고분자의 블렌드에 있어서 개질에 대한 연구가 더 수행되어진다면 합성고무에서 나타나는 부족한 가공성, 내산화 안정성, 접착성 등을 크게 향상시킬 수 있을 것이며 아울러 EVA의 융해유동성을 이용한 고무제품 가공공정의 연속화가 가능하게 될 것이다.

• 한국동물위생학회지
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• 제32권3호
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• pp.189-200
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• 2009

The purpose of this study was to evaluate the effect of a subsequent infection of PCV2 on piglets with PEDV. In clinical signs, the signs observed in dual-infected with PEDV and PCV2 piglets and alone infected with PEDV piglets ranged from diarrhoea to vomiting and dehydration. Dual-infected piglets developed signs of anorexia, vomiting and watery diarrhoea within 12 hpi. Nevertheless alone -infected piglets caused pasty diarrhea at first. In mortality, dual infections showed 25%, but alone -infections showed 8.3%, respectively. In gross findings, piglets dual-infected with PEDV and PCV2 appeared the severe findings of congestion, distension of lumen, milder curdes of undigested milk in stomach than those of single-infected piglets. In histopathological findings, piglets of dual-infection group appeared the more severe findings of villous atrophy and fusion, congesion, exfoliation, vacuolation, squamation, loss of cilia and proliferation of crypt. Significant (P<0.05) decrease in VH:CD ratio in dually infected piglets compared to piglets from alone-PEDV infections. In immunohistochemical findings, strong hybridization signals in dual-infected piglets observed moderate to severe villous atrophy or vacuolation with positive cells arranged continuously over the villi. In the lumen, exfoliated enterocytes were strongly positive in dual-infected piglets. A number of PEDV-positive cells in dual-infected pigs were significantly higher than that in alone PEDV-infected piglets.

• IMMUNE NETWORK
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• 제5권3호
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• pp.172-178
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• 2005

Background: Carcinoembryonic antigen (CEA) is well-known soluble tumor marker frequently detectable in peripheral blood of carcinoma patients and considered as good target for antigen-specific immunotherapy. However, it is known that the induction of immune response to CEA is very difficult because CEA is a self-antigen expressed in fetal cells and weakly expressed in normal colorectal epithelial cells. To enhance anti-tumor immunity specific for CEA, recombinant CEA protein was modified using listeriolysin O (LLO) for endosomal lysis and trans activator of transcription (Tat) domain for transducing extracellular proteins into cytoplasm. Methods: After immunization using dendritic cells pulsed with Tat-CEA, both Tat-CEA and LLO, and both Tat-CEA and Tat-LLO, antibody titer to CEA and LLO, cytotoxic T lymphocyte activity and the frequency of IFN-${\gamma}$ producing T lymphocytes were measured. Results: Immunization using DC pulsed with both Tat-CEA and Tat-LLO protein showed the increasement of production of CEA-specific antibody in serum, cytotoxic T lymphocyte activity, the frequency of IFN-${\gamma}$ secreting T cells, compared with DC pulsed with both Tat-CEA and LLO. Furthermore the ratio of CD8+T cell to $CD4^+$ cell among CEA-specific T cells was increased in group pulsed with both Tat-CEA and Tat-LLO. Conclusion: These results suggested that DC vaccine using Tat-LLO could be used for the development of effective immunotherapy for the treatment of tumor.