Browse > Article

Effects of porcine circovirus type 2 on the pathogenesis of porcine epidemic diarrhoea virus infection in piglets - II. Clinical sign, histopathological lesion and immunohistochemical finding -  

Jin, Wen (School of Veterinary Medicine, Kangwon National University)
Kim, Yeong-Hun (School of Veterinary Medicine, Kangwon National University)
Han, Jeong-Hee (School of Veterinary Medicine, Kangwon National University)
Publication Information
Korean Journal of Veterinary Service / v.32, no.3, 2009 , pp. 189-200 More about this Journal
Abstract
The purpose of this study was to evaluate the effect of a subsequent infection of PCV2 on piglets with PEDV. In clinical signs, the signs observed in dual-infected with PEDV and PCV2 piglets and alone infected with PEDV piglets ranged from diarrhoea to vomiting and dehydration. Dual-infected piglets developed signs of anorexia, vomiting and watery diarrhoea within 12 hpi. Nevertheless alone -infected piglets caused pasty diarrhea at first. In mortality, dual infections showed 25%, but alone -infections showed 8.3%, respectively. In gross findings, piglets dual-infected with PEDV and PCV2 appeared the severe findings of congestion, distension of lumen, milder curdes of undigested milk in stomach than those of single-infected piglets. In histopathological findings, piglets of dual-infection group appeared the more severe findings of villous atrophy and fusion, congesion, exfoliation, vacuolation, squamation, loss of cilia and proliferation of crypt. Significant (P<0.05) decrease in VH:CD ratio in dually infected piglets compared to piglets from alone-PEDV infections. In immunohistochemical findings, strong hybridization signals in dual-infected piglets observed moderate to severe villous atrophy or vacuolation with positive cells arranged continuously over the villi. In the lumen, exfoliated enterocytes were strongly positive in dual-infected piglets. A number of PEDV-positive cells in dual-infected pigs were significantly higher than that in alone PEDV-infected piglets.
Keywords
PEDV; PCV2; Dual-infected; Piglets;
Citations & Related Records
연도 인용수 순위
  • Reference
1 박남용, 조경요 조성수, 하영공. 1993. 돼지 유행성 설사 바이러스의 분리 동정. 대한수의학회지 29: 360-365
2 Allan G, Meehan B, Todd D, Kennedy S, McNeilly F, Ellis J, Clark EG. Harding J, Espuna E, Botner A, Charreyre C. 1998. Novel Porcine círcoviruses from pigs with wasting diseasesyndromes. Vet Rec 142(17): 467-468   PUBMED
3 DeBouck P, Pensaert M, Coussement W. 1981. The pathogenesis of an enteric infectÎon in pigs experi-mentally induced by coronavirus-Iike agent CV777. Vet Microbiol 6: 157-165   DOI   ScienceOn
4 DeBouck P, Pensaert M. 1980. Experimental infection of pigs with a new porcine enteric coronavirus, CV777. Am J Vet Res 41: 219-223   PUBMED
5 Hirai T, Nunoya T. 2006. Infectivity of porcine circovirus 1 and circovirus 2 in primary porcine hepatocyte and kidney cell cultures. J Vet Med Sci 68(2): 179-182   DOI   ScienceOn
6 Krakowka S, Ellis JA, McNeilly F, Ringler S, Rings DM, Allan G. 2001. Activation of the irnmune system is the pivotal event in the production of wasting disease in pigs infected with porcine circovirus 2 (PCV2). Vet Pathol 38: 31-42   DOI   ScienceOn
7 Kyriakis SC, Saoulidis K, Lekkas S, Miliotis ChC, Papoutsis PA, Kennedy S. 2002. The effects of immunomodulation on the cIinical and pathological expression of postweaning multisystemic wasting syndrome. J Comp Pathol 126: 38-46   DOI   ScienceOn
8 Pensaert MB and DeBouck P. 1978. A new coronavirus-like particle associated with diarrhea in swine. Arch Virol 58: 243-247   DOI   ScienceOn
9 Pensaert MB, DeBouck P, Reynolds DJ. 1981. An immunoelectron microscopic and immunofluorescent study on the antigenic relationship between thε coronavlruslike agent CV777 and several corona-viruses. Arch Virol 68: 45-52   DOI   ScienceOn
10 Pensaert MB. 2000. Transmissible gastroenteritis and porcine respiratory corona virus. In: Disease of swine, 8 eds. Iowa State Univ Press, Ames, Iowa, USA: 295-325
11 West KH, Bystrom JM, Wojnarowicz C, Shantz N, Jacobson M, Allan GM, Haines DM, Clark EG, Krakowka S, McNeIlly F, Konoby C, Martin K, ElIis JA. 1999. Myocarditis and abortion associated with intrauterine infection of sows with porcine circovirus 2. J Vet Diagn Invest 11: 530-532   DOI   PUBMED   ScienceOn
12 律田知幸. 1999. 隊流行性下痢. 豚病學, 第四版, 近代出版, 東京: 260-266
13 Rosell C, Segalees J, Rovira A. 2000. Porcine circovirus type 2 and postweaning multisystemic wasting syndrome already present in Spain in 1986. Proc. 16th IPVS Cong., Melbourne, Australia, pp. 631
14 Sueyoshi M, Tsuda T, Yamazaki K, Yoshida K, Nakazawa M, Sato K, Minami T, Iwashita K, Watanabe M, Suzuki Y. 1995. An immunohistochemical investigation of porcine epidemic diarrhea. J Comp Path 113(1):59-67   DOI   ScienceOn
15 박봉균, 한경수, 류광수, 김준영, 정현규.1998. 돼지 유행성 설사병(porcine epídεmíc diarrhea) 의 상재화에 대한 혈청학적 증명. 대한수의학회지 38: 818-822
16 김종만, 허원, 권창희 김종염 우승룡.1998. 난황항체를 이용한 돼지 대장균 설사증 방제기법 개발 I. 대장균 pilus 항원과 LT로 연역시컨 닭의 면역반응. 대한수의학회지 38: 829-836
17 Chasey D, Cartwright SF. 1978. Virus-like particles associated with porcine epidemic diarrhea. Res Vet Sci 25: 255-256   PUBMED
18 Krakowka S, Ellis JA, Meehan B, Kenlledy S, McNeilly F, Allan G. 2000. Viral wasting syndrome of swine: experimental reproduction of postweaning multisystemic wasting syndrome in gllotobiotic swine by coinfection with porcine circovirus 2 and porcine parvovirus. Vet Pathol 37: 254-263   DOI   ScienceOn
19 황의경, 김재훈, 진영화, 배유찬, 윤순식, 박최규, 권창화, 윤용덕, 엠 액커만.1994. 돼저 流行性. 泄瀉症의 最近 發生 動向. 農業科學論文集 36: 587-596
20 Jung K, Ha Y, Ha SK, Kim J, Choi C, Park HK, Kim SH, Chae C. 2006. Identification of porcine cìrcovirus type 2 in retrospective cases of pigs naturally infected with porcine epidemic diarrhoea vírus. Vet J 171(1 ):166-168   DOI   ScienceOn
21 Pijpers A, van Nieuwstadt AP, Terpstra C, Verheijden JH. 1993. Porcine epidemic virus as a cause of persistent diarrhea in a herd of breeding and finishing pigs. Vet Rec 132(6):129-131   DOI   PUBMED   ScienceOn
22 Kweon CH, Kwon BJ, Jung TS. 1993. Isolation of porcine epidemic diarrhea virus (PEDV) in Korea. Korean J Vet Res 33: 249-254
23 Coussement W, Ducatelle R, DeBouck P. 1982. Pathology of experimental CV777 coronavirus enteritis in piglets. I. Histological and histochemical study. Vet Pathol 19(1): 46-56   DOI   ScienceOn
24 Darwich L, Segalees J, Mateu E. 2004. Pathogenesis of postweaning multisystemic wasting syndrome caused by porcine circovirus 2 : an immune riddle. Arch Virol 149: 857-874   DOI   ScienceOn
25 EIIis JA, Bratanich A, Clark EG, Allan G, Meehan B, Haines DM, Harding J, West KH, Krakowka S, Konoby C, Hassard L, Martin K, McNeilly F. 2000. Coinfection by porcine acquired postweaning multisystemic wasting syndrome. J Vet Diagn Invest 12: 21-27   DOI   PUBMED   ScienceOn
26 Allan GM, Ellis JA. 2000. Porcíne circoviruses: a review. J Vet Diagn Invest 12(1): 3-14   DOI   PUBMED   ScienceOn
27 Guscetti F, Bemasconi C, Tobler K, Van Reeth K, Pospischil A, Ackermann M. 1998. Immunohistochemical detection of porcine epidemic diarrhea virus compared to other methods. Clin Diagn Lab lmmunol 5(3): 412-414
28 Takahashi K, Okada K, Ohshima K. 1983. An outbreak of swine diarrhea of a new-type associated with coronavirus-like particIes in Japan. Jpn J Vet Sci 45: 829-832   DOI   ScienceOn
29 Pensaert MB. 1999. Porcine epidemic diarrhea. In: Disease of swine, 8 eds. Iowa State Univ Press, Ames, Iowa, USA: 179-185
30 Kim O, Chaε C, Kweon CH. 1999. Monoclonal antibodybased immunohistochemical detection of porcine epidemic diarrhea virus antigen in formalin-fixed, paraffin-embedded intestinal tissues. J Vet Diagn Invest 11 (5): 458-462   DOI   PUBMED   ScienceOn