• 식물병연구
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• 제28권1호
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• pp.46-50
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• 2022

In June 2017, in Gangneung, Gangwon Province, South Korea, green pea pods exhibited post-harvest rot symptoms. The fungus was isolated from infected pea pods and cultured on potato dextrose agar for identification. The morphological characteristics were examined, sequences of the internal transcribed spacer region and the β-tubulin (βtub) gene were analyzed, and the pathogenicity was confirmed according to Koch's postulates. The morphology, phylogenetic analysis, and pathogenicity tests confirmed that Sclerotinia sclerotiorum was the causal agent. This study reports the first case of post-harvest green pea pod rot caused by S. sclerotiorum in Korea.

• 한국작물학회지
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• 제50권2호
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• pp.112-119
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• 2005

The effects of Rhizobium inoculant, nitrogen, phosphorus, and molybdenum on nodulation, dry matter production, yield attributes, pod and seed yields, protein and phosphorus contents in seed of pea (pisum sativum) var. IPSA Motorshuti-3 were assessed by a field experiment. Among the treatments Rhizobium inoculant in combination with 25kg P and 1.5kg Mo/ha performed best in recording number of nodules/plant, total dry matter yield, number of pods/plant, number of seeds/pod, 1000-seed weight, green pod yield, green and mature seed yields of pea. The highest green pod yield of 15.37 t/ha ($97.05\%$ increase over control) and green seed yield of 9.6t/ha ($69.31\%$ increase over control) were obtained by inoculating pea with Rhizobium inoculant in association with 25kg P and 1.5 Mo/ha. The effects of 60 or 120kg N/ha were comparable to Rhizobium inoculant in most cases. There were positive correlations among yield attributes, yield, protein and phosphorus contents in seeds of pea. From the viewpoint of yield attributes, yield, and seed quality, application of Rhizobium inoculant along with 25kg P and 1.5kg Mo/ha was considered to be the balanced combination of nutrients for achieving the maximum output from cultivation of pea in Shallow-Red Brown Terrace Soil of Bangladesh.

• 한국작물학회지
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• 제51권6호
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• pp.534-538
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• 2006

The effects of Rhizobium inoculant, compost, and nitrogen on nodulation, growth, dry matter production, yield attributes, and yield of pea (Pisum sativum) var, IPSA Motorshuti-3 were assessed by a field experiment. Among the treatments Rhizobium inoculant alone performed best in recording number and dry weight of nodules/plant. The highest green seed yield of 8.38 ton/ha (36.9% increase over control) and mature seed yield of 2.97 ton/ha (73.7% increase over control) were obtained by the application of 90 kg N/ha. The effects of 60 kg N/ha, Rhizobium inoculant alone and Rhizobium inoculant along with 5 ton compost/ha were same as the effect of 90 kg N/ha in recording plant height, root length, dry weight of shoot, and root both at preflowering and pod filling stages, number of mature pods/plant, number of mature seeds/pod, 1000-seed weight, green, and mature seed yields of pea.

• 한국작물학회지
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• 제48권6호
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• pp.447-451
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• 2003

풋완두 추파재배시 월동 전에 알맞은 입모와 생육량을 확보해야한다. 그리고 초봄에 저온, 서리 또는 PE. 필름 멀칭내 고온 등의 피해를 회피하여 적기에 순화하여야한다. 1. 완두 생육기간동안 기온이 높은 여수지역이 나주지역에 비해 각 생육단계별 소요일수가 짧고 생장량이 많으며, 유효분지수가 많고 유효분지의 착생절위와 주경과 유효분지의 제1착협절위가 높으며, 개체당 협수와 협당립수가 많고 협장이 길었다. 2. 파종적기는 결주율, 생육, 수량 등을 고려해 볼 때 여수와 나주 지역 모두 11월 중순이었다. 3. 안정적인 순화시기는 결주율과 순화시 피해율이 가장 낮고 유효분지수와 개체당 협수가 많아 수량이 가장 많은 3월 상순이었다.

• 분석과학
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• 제35권4호
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• pp.153-160
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• 2022

A green spectrophotometric method for the determination of cyanide has been proposed using, a green reagent, aqueous extract of blue butterfly pea. The test tube was filled with anthocyanin rich extract (pH 6) and cyanide solution. The reaction was kept constant for 10 minutes at room temperature. The reaction mixture changed color from blue to green as the amount of CN-ions increased. The 620 nm peak intensity increased with CN concentration. Therefore, this wavelength was used for all cyanide analyses. The cyanide calibration curve had a linear range of 0.25-1.00, 1.00-4.00, and 4.00-10.00 mg/L, with a satisfactory correlation coefficient of 0.99 and a LOD of 0.57 mg/L. The recovery ranged from 8.33 to 76.94 percent, indicating that this method is inaccurate at low cyanide concentrations. The intra-day and intermediate precision relative deviations were 0.391-0.871 % and 1.112-1.583 %. An H-bond forms between the C-4 group of the B-carbonyl ring and the HCN molecule according to the B3LYP/TZVP calculation. The method is convenient for cyanide concentrations above the LOQ of 1.09 mg/L, cost-effective, and capable of reducing toxic solvents with acceptable precision. The method could also be used to detect total cyanide in biological, environmental, and industrial waste samples.

• Reproductive and Developmental Biology
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• 제35권3호
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• pp.239-245
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• 2011

The overexpression of Phosphoprotein Enriched in Astrocytes (PEA15) gene is commonly found in human diabetic patients. The overexpression of this gene in skeletal muscle and fat tissues have been reported to cause insulin resistance, thereby impairing insulin stimulated glucose uptake. We introduced a gene of mouse PEA15 (mPEA15) and enhanced green fluorescent protein (EGFP) into fertilized one cell pig zygotes using microinjection, and produced a piglet that showed overexpression of mPEA15 in the muscle tissues and expression of EGFP in the ear tissues and hooves. RT-PCR RFLP, southern blot and FISH analysis showed that the tissues carried the transgene. Real-time RT-PCR and western blots demonstrated that PEA15 gene was overexpressed in the various tissues and muscle tissues, respectively. These fads suggest that expression vector system is normally expressed in the transgenic (TG) pigs. To use as animal diseases model for type 2 diabetes, further study is necessary to confirm whether diabetes occur in these TG pigs, especially insulin resistance.

• 한국유기농업학회지
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• 제19권2호
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• pp.255-272
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• 2011

우리나라 유기채소는 대부분 시설재배에서 생산되고 있으나, 그 동안 시설재배는 다수확을 목표로 과다한 유기자재 투입에 의한 토양염류 증가로 인한 토양 내 문제점이 발생되고 있다. 따라서 본 연구에서는 고추 무경운 시설 유기재배 시 녹비작물 재배 효과를 검토하기 위하여 본 시험을 수행하였다. 1.녹비작물의 생육 및 수량 월동 녹비작물을 파종 74일 후에 조사한 결과 건물 수량은 10a당 호밀은 720kg, 보리는 530kg, 헤어리베치는 230kg, 완두콩과 잡초는 240kg 수준이었으며, 1일 생체중으로 10a당 호밀과 보리는 100kg, 헤어리베치는 65kg 정도 하루에 증가되었다. 녹비작물을 파종하지 않은 무처리와 발아율이 낮은 완두콩 재배구에서 빈 공간에 7종의 잡초가 발생되었는데, 쇠별꽃과 황새냉이 2종의 잡초 발생량이 99% 이상을 점유 건물수량은 135kg 수준이었다. 2.녹비작물의 무기성분 녹비작물 식물체의 질소농도는 완두콩과 헤어리베치가 4.5%, 보리와 호밀은 3~4% 수준이었다. 식물체내 칼리와 칼슘, 마그네슘의 함량도 완두콩과 헤어리베치가 호밀, 보리보다 높은 경향을 보였다. 녹비작물의 식물체내 인산 함량과 칼슘 그리고 마그네슘 함량은 녹비의 종류에 관계없이 1% 내외였으나, 칼륨은 식물체의 무기성분이 4~5% 수준으로 다량 원소 중 가장 높게 나타났다. 녹비작물의 지상부가 체내에 고정한 총 질소량이 10a당 호밀과 보리는 20.6~22.1kg으로 헤어리베치, 완두콩+쇠별꽃 보다 2배 이상 많았다. 인산과 칼리의 고정량도 호밀과 보리는 6.3~8.4kg/10a으로 헤어리베치와 완두콩+쇠별꽃에 비하여 2.7배 정도 많았고, 칼리의 고정량도 호밀과 보리는 24.7~28kg/10a으로 헤어리베치와 완두콩+쇠별꽃 보다 2배 정도 많았다. 그리고 녹비작물에 의하여 흡수된 칼슘량은 10a당 2~3kg 수준이었으며 마그네슘은 1.7~2.6kg 수준이었다. 3.녹비작물과 고추 재배에 따른 토양 화학성 변화 녹비작물 재배 토양의 pH는 무처리를 포함하여 재배 전 토양에 비하여 증가되었다. 녹비작물 재배 토양의 염류농도는 감소되었으며, 보리녹비를 제거한 토양의 염류농도는 현저하게 감소되었다. 호밀과 헤어리베치 재배 토양의 유기물함량은 변함이 없었으나, 무처리와 보리, 완두콩재배 토양에서는 감소되었으며, 녹비를 환원하여 고추를 재배한 토양의 유기물 함량은 호밀과 헤어리베치 재배 토양에서 감소되었다. 녹비작물 재배 토양의 총질소 함량은 감소되었으나, 녹비를 환원하여 고추를 재배한 토양의 총질소 함량은 증가되었다. 녹비작물 재배토양과 녹비를 환원 고추를 재배한 토양의 유효인산 함량은 감소되었다. 녹비작물 재배 토양의 양이온친환용량은 감소되었으나, 녹비를 환원하여 고추를 재배한 토양에서 양이온 치환용량이 증가되었다. 토양의 K, Ca, Mg 함량은 녹비작물 재배 토양과 녹비 환원 후 고추를 재배한 토양에서 감소되었다. 4.녹비작물 재배지 무경운 유기재배 고추의 생육 및 수량 녹비작물 재배 토양에 무경운으로 재배한 고추의 생육은 녹비 품종간에는 차이가 적었다. 고추 수확과수는 녹비작물을 재배하지 않은 무처리와 녹비 건물량이 적은 보리 예취이용구와 완두콩 및 헤어리베치 재배지가 보리와 호밀 재배지에 비하여 수확과수가 현저하게 많았다. 고추의 수량은 보리를 예취하여 이용한 처리와 녹비작물을 재배하지 않은 무처리 및 녹비량이 적은 완두콩과 헤어리베치 재배지가 화본과 녹비작물 재배지보다 많았다.

• 한국작물학회지
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• 제45권2호
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• pp.93-96
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• 2000

The present study was performed to obtain the basic information about growth and quality related characteristics and optimum harvesting time for podedible pea which is a new crop in Korea but believed to have a great deal of potentials for both domestic and overseas markets. They can be consumed either as a fresh succulent vegetable or as tender green pods. The daily green pod yield of pod-edible peas started to increase from ten days after flowering and the maximum yield was recorded on 26 days after flowering. Ninety percent of pod yields could be harvested from 16 to 36 days after flowering. Mean green pod yield for the tested varieties was approximately 8.0 t/ha. Total vitamin C content of pod-edible peas showed continuously decreasing trends from five days after flowering. The highest sucrose content was obtained at ten days after flowering. The highest panel score based on sweetness, chewiness, and hardness for the processed green pods was shown at 10-15 days after flowering in all varieties tested, indicating that the optimum harvesting time for pod-edible peas was considered to be 10-15 days after flowering.

• Applied Biological Chemistry
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• 제29권4호
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• pp.399-406
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• 1986

2-Acylaminobenzothiazole derivatives were synthesized from 2-aminobenzothiazole and acylchloride. Benzothiazolylurea derivatives were syntesized from 2-minobenzothiazole and phenylisocyanate. The products were identified by UV, IR, $^1H-NMR$, $^{13}C-NMR$ spectra with 2-acetamidobenzothiazole(I), 2-propionamidobenzothiazole(II), 2-butamidobenzothiazole(III), 2-benzamidobenzothiazole(IV). The compounds were tested for their phytotoxicity on the germination and seedling growth of rice, radish and green pea plants, It was found that treatment of 500ppm concentration each of 2-acetamidobentothiazole, 2-propionarmidobenzothiazole and 2-butamidobenzothiazole strongly inhibited of seedling growth of the radish and green pea.

• Journal of Plant Biotechnology
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• 제7권4호
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• pp.233-240
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• 2005

Slices of embryonic axis of mature pea (Pisum sativum L. cv. Green Arrow) seeds were used as explant. Transformation of explants was done via Agrobacterium tumefaciens bearing vector pBI-P5CS construct. The best results for inoculation of explants were obtained when they were immersed for 90 s at a concentration of $6{\times}10^8$ cell $ml^(-1)$ of bacterial suspension. Transformed pea plants were selected on $50\;mg\;l^(-1)$ kanamycin and successful transformants were confirmed by PCR and blotting. Transgenic plants were further analyzed with RT-PCR to confirm the expression of P5CS. Transgenic plants and non-transgenic plants were treated with different concentrations of NaCl 0 (control), 100, 150 and 200 mM in culture medium. Measurement of proline content indicated that transgenic plants produced more amino acid proline in response to salt in comparison with non-transgenic plants. Photosynthetic efficiency in transgenic plants under salt-stress was more than that of non-transgenic plants.