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Salt Tolerance in Transgenic Pea (Pisum sativum L.) Plants by P5CS Gene Transfer  

Najafi F. (Department of Biology, Teacher Training University)
Rastgar-jazii F. (National Research Center for Genetic Engineering and Biotechnology)
Khavari-Nejad R. A. (Department of Biology, Teacher Training University)
Sticklen M. (Department of Crop and Soil Science, Michigan State University)
Publication Information
Journal of Plant Biotechnology / v.7, no.4, 2005 , pp. 233-240 More about this Journal
Abstract
Slices of embryonic axis of mature pea (Pisum sativum L. cv. Green Arrow) seeds were used as explant. Transformation of explants was done via Agrobacterium tumefaciens bearing vector pBI-P5CS construct. The best results for inoculation of explants were obtained when they were immersed for 90 s at a concentration of $6{\times}10^8$ cell $ml^(-1)$ of bacterial suspension. Transformed pea plants were selected on $50\;mg\;l^(-1)$ kanamycin and successful transformants were confirmed by PCR and blotting. Transgenic plants were further analyzed with RT-PCR to confirm the expression of P5CS. Transgenic plants and non-transgenic plants were treated with different concentrations of NaCl 0 (control), 100, 150 and 200 mM in culture medium. Measurement of proline content indicated that transgenic plants produced more amino acid proline in response to salt in comparison with non-transgenic plants. Photosynthetic efficiency in transgenic plants under salt-stress was more than that of non-transgenic plants.
Keywords
${\Delta}^1$-pyrroline-5-carboxylate synthetase; P5CS; Pea; Salt Tolerance;
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