• Title/Summary/Keyword: Green Transformation

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Plastid Transformation in the Monocotyledonous Cereal Crop, Rice (Oryza sativa) and Transmission of Transgenes to Their Progeny

  • Lee, Sa Mi;Kang, Kyungsu;Chung, Hyunsup;Yoo, Soon Hee;Ming Xu, Xiang;Lee, Seung-Bum;Cheong, Jong-Joo;Daniell, Henry;Kim, Minkyun
    • Molecules and Cells
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    • v.21 no.3
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    • pp.401-410
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    • 2006
  • The plastid transformation approach offers a number of unique advantages, including high-level transgene expression, multi-gene engineering, transgene containment, and a lack of gene silencing and position effects. The extension of plastid transformation technology to monocotyledonous cereal crops, including rice, bears great promise for the improvement of agronomic traits, and the efficient production of pharmaceutical or nutritional enhancement. Here, we report a promising step towards stable plastid transformation in rice. We produced fertile transplastomic rice plants and demonstrated transmission of the plastidexpressed green fluorescent protein (GFP) and aminoglycoside 3′-adenylyltransferase genes to the progeny of these plants. Transgenic chloroplasts were determined to have stably expressed the GFP, which was confirmed by both confocal microscopy and Western blot analyses. Although the produced rice plastid transformants were found to be heteroplastomic, and the transformation efficiency requires further improvement, this study has established a variety of parameters for the use of plastid transformation technology in cereal crops.

Virus Resistant and Susceptible Transgenic Nicotiana benthamiana Plants Expressing Coat Protein Gene of Zucchini green mottle mosaic virus for LMO Safety Assessment

  • Kim, Min-Jea;Choi, Sun-Hee;Kim, Tae-Sung;Park, Min-Hye;Lim, Hee-Rae;Oh, Kyung-Hee;Kim, Tae-San;Lee, Min-Hyo;Ryu, Ki-Hyun
    • The Plant Pathology Journal
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    • v.20 no.3
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    • pp.206-211
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    • 2004
  • Transgenic Nicotiana benthamiana plants harboring coat protein (CP) gene of Zucchini green mottle mosaic virus (ZGMMV) were generated for virus-resistant screening and complementation analysis of related viruses for environmental safety assessment (SA) of living modified organism (LMO) purposes. Transformation of leaf disc of N.benthamiana was performed by using Agrobacterium-mediated method and the pZGC-PPGA748 containing the ZGMMV CP and NPTII genes. Two kinds of transgenic homozygous groups, virus-resistant and virus-susceptible N.benthamiana lines, were obtained by screening of challenging homologous virus for Tl generations. These two pathologically different lines can be useful for host-virus interactions and LMO environmental SA.

The Low Carbon & Green Growth Policy and Green Life-Style, The Practical Implication and Vision on Family (저탄소녹색성장정책과 녹색생활양식, 가족에 대한 실천적 함의와 전망)

  • Choi, Youn-Shil;Sung, Mi-Ai
    • Journal of the Korean Home Economics Association
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    • v.49 no.1
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    • pp.79-91
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    • 2011
  • The purposes of this study were firstly to explore the practical implications that of 'low carbon and green growth' policy, which is at the top of the Government's agenda provides to family, and secondly to propose some visions for a future based on those implications. The results of this study were as follows: Firstly, in terms of a global perspective, there is now a worldwide trend towards the adoption of 'low carbon and green growth' policies. Secondly, the Government-driven 'green growth policy' demands a total transformation, that is, revolution, not only in terms of our industries, but also in terms of our mentality and ordinary life. Thirdly, the driving force for this life revolution lies in having green life style, and the family is the primary agent for making the green life style a practical reality.

Efficiency of transformation mediated by Agrobacterium tumefaciens using vacuum infiltration in rice (Oryza sativa L.)

  • Safitri, Fika Ayu;Ubaidillah, Mohammad;Kim, Kyung-Min
    • Journal of Plant Biotechnology
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    • v.43 no.1
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    • pp.66-75
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    • 2016
  • Agrobacterium-mediated gene transfer has recently been developed to improve rice transformation. In this study, 3 different transformation methods were tested including soaking, co-cultivation, and vacuum infiltration. Agrobacterium tumefaciens GV3101 harboring the binary vector pGreen:: LeGSNOR was used in this experiment. This study aimed to identify the most appropriate method for transferring LeGSNOR into rice. Vacuum infiltration of the embryonic calli for 5 min in Ilpum resulted in high transformation efficiency based on confirmation by PCR, RT-PCR, and qRT-PCR analyses. In conclusion, we described the development of an efficient transformation protocol for the stable integration of foreign genes into rice; furthermore, the study results confirmed that PCR is suitable for efficient detection of the integrated gene. The vacuum infiltration system is a potentially useful tool for future studies focusing on transferring important genes into rice seed calli, and may help reduce time and effort.

SH-wave propagation in a heterogeneous layer over an inhomogeneous isotropic elastic half-space

  • Kakar, Rajneesh
    • Earthquakes and Structures
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    • v.9 no.2
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    • pp.305-320
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    • 2015
  • The present paper is devoted to study SH-wave propagation in heterogeneous layer laying over an inhomogeneous isotropic elastic half-space. The dispersion relation for propagation of said waves is derived with Green's function method and Fourier transform. As a special case when the upper layer and lower half-space are homogeneous, our derived equation is in agreement with the general equation of Love wave. Numerically, it is observed that the velocity of SH-wave increases with the increase of inhomogeneity parameter.

Green Bonds Driving Sustainable Transition in Asian Economies: The Case of India

  • PRAKASH, Nisha;SETHI, Madhvi
    • The Journal of Asian Finance, Economics and Business
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    • v.8 no.1
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    • pp.723-732
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    • 2021
  • On September 25, 2015, 193 countries of the United Nations (UN) General Assembly, signed the 2030 Agenda to work towards attaining 17 Sustainable Development Goals (SDGs) and its associated 169 targets and 232 indicators. With one of the largest renewable energy programs, India is well-poised to be a role model for low-carbon transformation to other Asian countries. However, bridging the financing gap is critical to ensure that the country meets its SDG targets. Though the SDGs identified by the UN are broad-based and interdependent, for ease of analysis we have grouped them into five themes - people, planet, prosperity, peace, and partnership - based on existing UN models. This paper investigates the financing gap for 'green' projects linked to planet-related SDG targets in India. It builds an argument for utilizing green bonds as an instrument to bridge the gap. After establishing the potential of green bonds in raising the finance to meet India's planet-related SDG targets, we look at the current policy landscape and suggest recommendations for successful execution. The paper concludes that deepening of the corporate fixed income securities market and firming up guidelines in line with India's climate action plans are inevitable before green bonds can be considered a viable financing option.

Analysis on Thermomechanical Response to Tensile Deformation of GaN Nanowires (GaN 나노와이어의 인장 변형에 의한 열기계적 거동 해석)

  • Jung, Kwangsub;Zhou, Min;Cho, Maenghyo
    • Journal of the Computational Structural Engineering Institute of Korea
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    • v.25 no.4
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    • pp.301-305
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    • 2012
  • In this work the mechanical behaviors of GaN nanowires are analyzed during tension, compression, and unloading deformations. The thermal conductivity of the nanowires at each deformed state is evaluated using an equilibrium Green-Kubo approach. Under tensile loading, the [0001]-oriented nanowires with hexagonal cross-sections undergo a phase transformation from wurtzite to a tetragonal structure. The phase transformation is not observed under compressive loading. The thermal conductivity decreases on going from compressive strains to tensile strains. The strain dependence of the thermal conductivity results from the relaxation time of phonon. A reverse transformation from the tetragonal structure to the wurtzite structure is observed during unloading. The thermal conductivities in the intermediate states are lower than the conductivity in the wurtzite structure at same strain. Such differences in the thermal conductivity between different atomic structures are mainly due to changes in the group velocity of phonon.

A Comparative Study of the Harmony of coloration according to Transformation of Color Area-Ratio in Traditional Korean Dress - On the Tone on Tone coloration - (색상 면적비 변화에 따른 한복배색의 조화감 비교 연구 - 톤 온 톤 배색을 중심으로 -)

  • Kang Kyung-Ja;Chu Mi-Seon;Paeng Suk-Kyung
    • Journal of the Korean Home Economics Association
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    • v.43 no.5 s.207
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    • pp.107-115
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    • 2005
  • The purpose of this research was to compare the harmony of tone on tone coloration according to transformation of color area-ratio in traditional Korean dress. The respondents were asked to evaluate 36 stimuli of the traditional Korean skirt and jacket with different color tone. The subjects were 83 female undergraduate students. The results are as follows. For red colors, combinations of vivid/dull, vivid/dark, and light/dark made a difference in the harmony of coloration according to the transformation of color area-ratio. For yellow colors, combinations of vivid/dark, light/dull, and light/dark made a difference in the harmony of coloration according to the transformation of color area-ratio. For green colors, combinations of vivid/dull, vivid/dark, light/dull, and light/dark made a difference in the harmony of coloration according to the transformation of color area-ratio.

Re-coloring Methods using the HSV Color Space for people with the Red-green Color Vision Deficiency (적록 색각 이상자를 위한 HSV색공간을 이용한 색변환 기법)

  • Kim, Hyun-Ji;Cho, Jae-Young;Ko, Sung-Jea
    • Journal of the Institute of Electronics and Information Engineers
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    • v.50 no.3
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    • pp.91-101
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    • 2013
  • This paper proposes a new re-coloring method for the people with the red-green color vision deficiency (CVD). These people have difficulty in discriminating the red and green colors since they abnormally perceive the hue and luminance value of the colors. We introduce a color transformation that adjusts the hue and luminance value in HSV color space. The color transformation is determined according to the severity of CVD. Our aim is to maintain the color differences in original image while maintaining the recolored image to be natural to the people with normal color vision. Experimental results show that the proposed method can yield more comprehensible images for the people with red-green CVD while maintaining the naturalness of the recolored images.