• Journal of Plant Biotechnology
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• v.39 no.1
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• pp.69-74
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• 2012

Using calli of $Muscari$ $armeniacum$ cv. 'Early Giant' that is monocotyledonous ornamental bulb crop with increasing demand in Korea, we carried out current studies to establish an in vitro multiple propagation protocol via somatic embryogenesis. We found that soft pale yellow green calli were induced from leaf explants cultured on all media containing 0.1~3.0 $mg{\cdot}L^{-1}$ auxins such as 1-naphthalene acetic acid (NAA) and 2,4-dichlorophenoxyacetic acid (2,4-D). However, induced calli showed vigorous growth only when they further transferred on same media containing 2,4-D, 4-amino-3,5,6-tri-chloropicolinic acid (picloram), or 3,6-dichloro-o-anisic acid (dicamba). Although frequency of somatic embryo induction depended on callus source and PGR composition in somatic embryo induction media, somatic embryogenesis was initiated on surface of proliferated calli after transferring on media with no PGR or 0.01 $mg{\cdot}L^{-1}$ NAA co-supplemented with various cytokinins such as $N^6$-benzylaminopurine (BAP). Highest number of embryo at 9.3 per callus clump was obtained when calli which were grown under 0.1 $mg{\cdot}L^{-1}$ picloram supplementation were sub-cultured on medium with 0.01 $mg{\cdot}L^{-1}$ NAA and 0.5 $mg{\cdot}L^{-1}$ BAP. In addition, morphological characteristics of somatic embryo were categorized into following nine phases: globular, biased heart, biased torpedo, early cotyledonary, middle cotyledonary, late cotyledonary, early sprouting, middle sprouting, and late sprouting embryos.

• Pediatric Infection and Vaccine
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• v.4 no.1
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• pp.97-105
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• 1997

Purpose : Aseptic meningitis mainly caused by enterovirus is common in pediatric population especially during summer & fall. Most of pediatric patients restore their health without any complications with proper management. Between May to August of 1996, Masan and surrounding areas of the Kyoungsangnamdo were epidemic areas for the aseptic meningitis. The purpose of this study was to determine causative virus and describe correlation between disease and clinical symptoms in aseptic meningitis patients and those with fever and characteristic rashes without apparent meningitis symptoms. Methods : Between May to August, 1996, 57 patients with high fever and characteristic feature of rashes were reviewed. From 22 cerebrospinal fluid & 57 stool obtained specimens, viral culture and detection of enterovirus RNA were conducted. Collected specimens were kept in $-30^{\circ}C$ environment until sending of specimens to labortory. The virus identified through indirect immunofluorescence. RT-PCR method was used to identify enterovirus RNA in cerebralspinal fluid. Results : 1) One hundred fifty five pediatric patients with viral infection required hospitalization. Disease occurred higher rate in male than female with ratio of 1.94:1. Examined patients' age ranged from 15days old to 15years old. But most of patients(74.8%) were under age of 5years old. The time of occurrence was between May to August of 1996. 2) All patients had high fever and physical symptoms in those patients include headache, vomiting, abdominal pain, diarrhea, and rashes. The rashes observed mainly in patients under age of 4 years and were predominantly commom patients under age of 18 months olds)<0.001). 3) Between sampled patients and non-sampled patients, clinical course was similar. Echovirus type 9 was cultivated in 41 out of 57 cases of collected stool specimens. RT-PCR that used on CSF showed positive results in 10 out of 22 cases. Three cases of positive cultivated of positive results in RT-PCR were echovirus type 9. Conclusions : Echovirus type 9 was thought to be the causative agent of aseptic meningitis that was prevalent throughout mid areas of Kyoungsangnamdo from May to August, 1996. Additionally causative agent that responsible for high fever with rashes without meningitis symptoms also thought to be the same echovirus type 9.

• Journal of the Korean Wood Science and Technology
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• v.19 no.1
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• pp.31-63
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• 1991

It was proved that in conventional kiln drying of disks piling position in the kiln exerted a great influence on drying rates, and the larger the variation of disk diameter, the more undulating drying rates of disks. While in vacuum drying disks there was no influence on drying rates. By the end-wrapping treatments and the radial direction of disks tangential surface stresses in the core of disks were slightly compressive in three species. In control disks the drying stresses distributed into one step-style that compressive stresses in the pith side of 6cm from pith were larger than those in the bark side, while in the disks end-wrapped with Korean paper the drying stresses distributed uniformly, because flow rates of free water in disks had no difference between heart-and sap-wood by obstruction of evaporating water from surface of disks by end-wrapping with Korean paper. And end-wrapping with Korean paper considerably restrained those. Tangential differential shrinkage stresses developed the maximum tensile stress near the bark and with approaching the pith the stresses gradually reduced and changed into compressive stresses in near the pith. At the end of vacuum drying the maximum tangential tensile stresses of disks end-wrapped with Korean paper were smaller than those of control disks, and critical moisture contents causing the V-shaped crack of disks end-wrapped with Korean paper were lower than those of control disks because of the set by obstruction of evaporating water of end-wrapping with Korean paper. In the experiment of vacuum drying stress distribution the disks end-wrapped with Korean paper or aluminum foil in three species were free from V-shaped cracks and control disks were defected very slightly by V-shaped cracks. And also disks end-wrapped with Korean paper were free from heart checks in Alnus japonica and Juglans sinensis, and heart checks were occurred very slightly in others. Especially, not to speak of disks end-wrapped with Korean paper, vacuum drying of disks end-wrapped with aluminum foil prevented effectively drying defects, moreover drying times could be shortened, that is. Ginkgo biloba, Alnus japonica, and Juglans sinensis disks could be dried from green to in-use moisture content in 110 hours, 272 hours, and 407 hours, respectively.

• Korean journal of applied entomology
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• v.50 no.2
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• pp.157-165
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• 2011

Enterobacteria were isolated in the gut of the predacious multicolored Asian ladybird beetle, Harmonia axyridis, and their effects to the development of H. axyridis were examined. Populations of H. axyridis in this experiment were collected from Kimjae at Cheonbuk province (JK population), Geumsan at Chungnam province (CK population) and laboratory population at Laboratory of Insect Physiology in Chungnam National University, Daejeon. Thirty-four enterobacteria isolates were purified and isolated from the digestive tract of H. axyridis, and a total of 4 strains were classified into group by analysis of 16S rRNA gene sequences. About 70% of total isolates were phylogenetic groups of Bacillus genus and Staphylococcus genus, and they were commonly separated from the digestive tract of H. axyridis. After investigating their susceptibility against antibiotics with 18 representative enterobacteria isolates, ofloxacin and penicillin were selected for examination in this study of their ability to inhibit the growth of all of isolates. In order to remove the enterobacteria from the aphids, ofloxacin and penicillin were given to the green peach aphid, Myzus persicae, and the turnip aphid, Lipaphis erysimi. These aphids were provided to H. axyridis as prey. The weight of pupa, developmental periods of each larval instar, the number of eggs and their hatching ratio of H. axyridis with treatment aphids were lower compared with non-treatment aphids. Staphylococcus saprophyticus is a representative enterobacteria and commonly isolated from the digestive tract of H. axyridis. In the absence of S. saprophyticus, the developmental periods of each larval instar increased; however, the weights of pupa, the number of eggs, and their hatching ratio decreased.

• Journal of Food Hygiene and Safety
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• v.26 no.1
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• pp.49-56
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• 2011

To investigate the toxicological effects of ${\beta}$-glucan, we performed basic studying on ${\beta}$-glucan in Sprague-Dawley (SD) rats. Standard endpoints in this study included mortality, clinical observations, changes of body weights, analysis on food consumption, ophthalmoscopic examination, hematologic examination, serum biochemistry, analysis of organ weights, gross anatomic pathology and histopathology. No clinical signs and mortality were observed in animals treated with beta-glucan throughout the experimental period. The average body weight of each treatment groups showed similar levels at end of experiment. There were no treatment-related changes in mortality, body weights changes, food consumption, ophthalmoscopic examination. Although there were statistically significant differences between the control and treated groups in some relative and absolute organ weights, and hematological and biochemical analysis, the data were in biologically normal ranges and did not show a dose-dependent manner. In the morphological change, hepatic tissue were not showed ballooning degeneration and irregular arrangement of hepatic cell in ${\beta}$-glucan treatment groups with control group. Also, organs weights (liver, heart, kidney and stomach) and hematological indices (WBC, RBC, Hb, Hct and Platelet) did not show statistically significant differences among the experimental groups. In summary of these results, there were no changes in mortality, mean body weight, clinical signs, food consumption. There were no changes in ophthalmological examination, hematology, blood chemistry, necropsy and histopathology. In conclusion, although further investigation of glucan should be performed in the functions registered in many ancient literatures, ${\beta}$-glucan is physiologically safe and may have potential as candidate food for human health.

• Korean Journal of Poultry Science
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• v.31 no.4
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• pp.265-271
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• 2004

A feeding trial was conducted to investigate the influence of feeding Lactobacillus reuteri culture (LR) on productive performance, intestinal microflora and availability in laying hens. Four hundred and eighty, Isa-Brown layers, 49 weeks of age, were fed diets supplemented with LR at the level of 0 (control), 0.1, 0.2, and $0.4\%$ of the diets for eight weeks. Egg production and egg weight were measured daily. Feed intake was weighed every two weeks. Egg quality was measured three times at the start, mid-term, and end of the experiment. Intestinal microflora were examined for Lactobacillus spp., E. coli and Salmonella at the end of the experiment. Overall egg production was the highest in $0.2\%$ LR (P<0.05), but that of $0.1\%$ or $0.4\%$ LR treatments did not significantly differ from that of control. Egg weight was significantly higher in LR feeding group than the control (P<0.05). Daily egg mass was significantly higher in $0.2\%$ and $0.4\%$ LR treatments compared to the control and $0.1\%$ LR (P<0.05). The number of jumbo and extra large eggs were increased in LR supplemented groups, especially in $0.1\%$ LR. Feed intake of layers fed LR supplemented diets tended to be lower than the control. However, feed conversion ratio significantly improved in LR supplemented groups (P<0.05). Availability of dry matter and crude protein improved significantly in $0.4\%$ LR treatment (P<0.05). But, those of ether extract and crude ash were not significantly different among treatments. Eggshell breaking strength and eggshell thickness were not significantly influenced by LR supplementation, and Haugh unit and yolk index were also similar to the control. Total number of Lactobacillus spp. in ileum and cecum fed LR supplemented diets were significantly higher than those of the control (P<0.05). There were no significant differences in intestinal E. coli and Salmonella in all treatments. Therefore, it is concluded that dietary supplementation of Lactobacillus reuteri culture can improve the laying performance, feed efficiency and intestinal Lactobacillus.

• Journal of the Korean Institute of Landscape Architecture
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• v.48 no.2
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• pp.34-44
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• 2020

The purpose of this study was to investigate the difference in perceptions between gardens and park functions as recognized by two groups, Group A and Group B, in order to confirm the distinction between concepts and functions and then establish the importance of individual functions. The AHP was used to analyze the importance of each group's perceptions by dividing them into garden and park, Group A and non-Group A, respectively. In Group A, the importance of garden functions were considered in descending order of importance to be cultural function, ecological function, and social function. In the general group, ecological function, cultural function, and social function also appeared, but in a different order of importance. As for the park functions, Group A recognized the importance of functions in a similar order of importance to the gardens: cultural function, ecological function, and social function. Group B thought that social function, ecological function, and cultural function have the same significance. At the major classification level, Group A and Group B emphasized the social function of the parks. Group A recognized the importance of the garden's cultural function as the most important, whereas the general group emphasized the importance of the garden's ecological function. As for the mid-class level, Group A recognized the aesthetic beauty, health, ecological environment protection, and water circulation as important functions of the garden. For Group B, the ecological environment protection, aesthetic beauty, water cycle, and health were important. The concepts and functions of gardens and parks are still largely mixed but are gradually becoming differentiated. As a follow-up study, it is important to systematically manage the functions of gardens by establishing design, construction, and monitoring DB techniques for the garden type and examine the hierarchy of various other gardens.

• Journal of Bio-Environment Control
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• v.22 no.3
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• pp.248-255
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• 2013

This study examined an automated irrigation technique by a frequency domain reflectometry (FDR) sensor for scheduling irrigation for tomato (Solanum lycopersicum L. 'Starbuck F1') cultivation aimed at avoiding effluent from an open hydroponic system with coir substrate containing different ratios of chip-to-dust (v/v) content. Specifically, the objectives were to undertake preliminary measurements of irrigation volumes, leachate volume, volumetric water content and electrical conductivity (EC) in the substrate, plant growth, fruit yield, and water use efficiency resulting from variation in chip content as an initial experiment. Commercial coir substrates containing different percentages of chips and dust (0 and 100%, 30 and 70%, 50 and 50%, or 70 and 30%), two-story coir substrates with different percentages of chips in the lower layer and dust in the upper layer (15 and 85%, 25 and 75%, or 35 and 65%), or rockwool slabs were used. The results showed that a negligible or no leachate was found for all treatments when plants were grown under a technique for scheduling non-drainage irrigation using a frequency domain reflectometry (FDR) sensor. Daily irrigation volume was affected by chip content in both commercial and two-story slabs. The highest plant growth, marketable fruit weight, and water-use efficiency were observed in the plants grown in the commercial coir slab containing 0% chips and 100% dust, indicating that the FDR sensor-auto-mated irrigation may be more useful for tomato cultivation in coir substrate containing 0% chips and 100% dust using water efficiently and minimizing or avoiding leachate and thus increasing yield and reducing pollution. Detailed experiment is necessary to closely focus on determining appropriate irrigation volume at each of irrigation as well as duration of each individual irrigation cycle depending on different physical properties of substrates using an automated irrigation system operated by the FDR sensor.

• Progress in Medical Physics
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• v.21 no.1
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• pp.113-119
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• 2010

Software for GafChromic EBT2 film dosimetry was developed in this study. The software provides film calibration functions based on color channels, which are categorized depending on the colors red, green, blue, and gray. Evaluations of the correction effects for light scattering of a flat-bed scanner and thickness differences of the active layer are available. Dosimetric results from EBT2 films can be compared with those from the treatment planning system ECLIPSE or the two-dimensional ionization chamber array MatriXX. Dose verification using EBT2 films is implemented by carrying out the following procedures: file import, noise filtering, background correction and active layer correction, dose calculation, and evaluation. The relative and absolute background corrections are selectively applied. The calibration results and fitting equation for the sensitometric curve are exported to files. After two different types of dose matrixes are aligned through the interpolation of spatial pixel spacing, interactive translation, and rotation, profiles and isodose curves are compared. In addition, the gamma index and gamma histogram are analyzed according to the determined criteria of distance-to-agreement and dose difference. The performance evaluations were achieved by dose verification in the $60^{\circ}$-enhanced dynamic wedged field and intensity-modulated (IM) beams for prostate cancer. All pass ratios for the two types of tests showed more than 99% in the evaluation, and a gamma histogram with 3 mm and 3% criteria was used. The software was developed for use in routine periodic quality assurance and complex IM beam verification. It can also be used as a dedicated radiochromic film software tool for analyzing dose distribution.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.51 no.3
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• pp.233-238
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• 2006

This study was conducted to evaluate growth characteristics, yield and feed value of newly bred domestic silage corn hybrids compared to foreign imported corn hybrid. Days to silking were $78{\sim}86$ days and longer than that of P3394 (Control). Kwangpyeongok, Cheonganok, and Cheongsaok were resistant to lodging. Such trait of stay-green of Kwanganok, Kwangpyeongok, Cheonganok, Cheongsaok, and Suwon159 was not different from that of P3394. Duruok, Kwangpyeongok, and Suwon159 were more resistant to Bipolaris maydis (southern corn leaf blight) than P3394. All of the silage corn hybrids were resistant to Exserohilum turcicum (northern corn leaf blight) and Smut. Ear ratios to total dry matter (%) of Cheonganok was similar to P3394. Ear lengths of domestic hybrids were similar or longer than that of P3394. Dry matter yield of domestic hybrids except Suwon19 and Jangdaok were similar to that of P3394. Total digestible nutrient (TDN) yields, Duruok, Kwangpyeongok, Cheonganok, and Cheongsaok were similar to P3394, but Kwanganok, Suwon19, Jangdaok and Suwon159 were lower than P3394. Acid detergent fiber(ADF), Suwon19 was lowest among all hybrids, and all hybrids except Suwon19 had values ranging from 44.7 to 49.0%, similarly with P3394. Neutral detergent fiber (NDF) values of the hybrids were similar to that of P3394, and ranged from 68.1 to 72.8%, except for Duruok which was lowest. Relative feed values (RFV) of Duruok, Kwangpyeongok, Cheonganok, Jangdaok, and Suwon159 had values ranging from 71.0 to 75.7 and were not significantly different from P3394. ADF was significantly negatively correlated with number of days to silking, number of days to maturity, and fresh matter yield, but was significantly positively correlated with resistance to corn borer. RFV showed a significantly positive correlations with no. of days to maturity and fresh matter yield, but significantly negative correlation with resistance to European corn borer. Therefore, indices for evaluation of feed value as well as production were important to select corn hybrids for silage. It is concluded that domestic hybrids tested in this study have high feed value as well as high production similar to those of imported corn hybrid.