• 생약학회지
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• 제47권2호
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• pp.143-149
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• 2016

Grateloupia elliptica has been reported to have the proliferation effect of dermal papilla cells (DPCs), which play important roles in the regulation of hair cycle. In the present study, we examined in vitro and in vivo hair growth-promoting effect of Grateloupia elliptica. When isolated rat vibrissa follicles were treated with extract of G. elliptica, the hair-fiber lengths of the vibrissa follicles significantly increased. Furthermore, the G. elliptica extract accelerated the telogen-angen transition in C57BL/6 mice. To investigate the molecular mechanisms of the G. elliptica extract on the proliferation of DPCs, we examined the activation of $wnt/{\beta}$-catenin signaling which is known to regulate hair follicle development, differentiation and hair growth. The G. elliptica extract activated $wnt/{\beta}$-catenin signaling via the increase of ${\beta}$-catenin and phospho-$GSK3{\beta}$. In addition, the G. elliptica extract increased the level of cyclin E and CDK2, while the level of $p27^{kip1}$ was decreased. These results suggest that the the G. elliptica extract may induce hair growth by proliferation of DPCs via cell-cycle progression and the activation of $Wnt/{\beta}$-catenin signaling.

• 한국어병학회지
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• 제18권2호
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• pp.147-156
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• 2005

본 연구에서는 주요 어병세균인 Staphylococcus sp., Streptococcus sp., E. tarda 및 V. anguillarum에 대한 30 종의 해조류 추출물의 항균활성을 검색하였다. 그 결과, C. officinalis, D. simplex, G. furcata, G. lanceolata 및 G. turuturu의 경우는 그람 양성균인 Staphylococcus sp.에 대하여, 그리고 S. thunbergii 및 P. morrowii 는 그람 음성균인 E. tarda와 V. anguillarum에 대하여 항균활성을 나타냄을 밝혔다. 그 중, 가장 우수한 항균활성을 나타낸 P. morrowii의 항균활성분획물인 90% MeOH 분획물은 E. tarda의 증식을 매우 효과적으로 저해하며, MIC는 1 mg/ml으로 측정되었다. 뿐만 아니라, 다양한 pH 조건하에서도 항균활성이 유지되었으며 분획물의 주요활성 성분은 할로겐 함유 phenol계 화합물일 것으로 추정된다.

• Fisheries and Aquatic Sciences
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• 제19권3호
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• pp.13.1-13.8
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• 2016

Reproductive phenological pattern on tetrasporophytes and carposporophytes of Grateloupia subpectinata thalli were observed at Yangyang, on the middle east coast of Korea from January to December 2013. DNA sequences of cox1 gene from 48 selected samples were compared to assure that sample collection is single species, and they were all identical G. subpectinata. Density, length and width of thalli were significantly changed monthly. The maximum of density, length and fresh weight occurred in September, and continuous growth was observed during the autumn and winter. Tetrasporophytes were grown dominantly in October and November, whereas carposporophytes were dominated in September and March. Upright thalli disappeared in June and July and then juveniles were abundant in August. There was a significant difference in length between tetrasporophytes and carposporophytes. These reproductive pattern and seasonality together with phenological data of G. subpectinata would provide valuable information about successful seedling supply for aquaculture of the species in the future.

• ALGAE
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• 제21권3호
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• pp.343-348
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• 2006

This study was conducted to screen in vitro angiotensin - I converting enzyme (ACE) inhibitory activities of methanol (MeOH) and aqueous extracts at 20°C and 70°C, respectively, prepared from twenty-six red algae obtained from the coast of Jeju Island in Korea. Among aqueous extracts at 20°C (20AE) from red algae Lomentaria catenata showed the strongest ACE inhibitory activity and Lithophyllum okamurae recorded the second highest activity. From MeOH extract at 20°C (20ME) Ahnfeltiopsis flabelliformis possessed the strongest ACE inhibitory activity. Remarkable activities from MeOH extracts at 70°C (70ME) were observed in Grateloupia filicina, Sinkoraena lancifolia and Grateloupia lanceolata. However, no significant activity was found in aqueous extracts at 70°C (70AE). The IC50 values, which are concentrations required to inhibit 50% activity of ACE, for ACE inhibitory activities of 20AE from Lithophyllum okamurae and L. catenata were 13.78 and 12.21 μg mL–1, respectively. The IC50 values of 20ME from A. flabelliformis and Laurencia okamurae were 13.84 and 106.15 μg mL–1. Those of the 70ME from Bonnemaisonia hamifera, Grateloupia filicina, Sinkoraena lancifolia, G. lanceolata, Gracilaria vermiculophylla and L. okamurae ranged from 25.82 to 124.69 μg mL–1.

• ALGAE
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• 제24권3호
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• pp.163-168
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• 2009

Grateloupia filicina (J.V. Lamouroux) C. Agardh (Halymeniaceae, Cryptonemiales, Rhodophyta) is an edible seaweed as well as an important source of carrageenan. In the present study, attempt has been made to develop a suitable protocol for effective regeneration of the seaweed and the rapid multiplication of the desired varieties. The young upright thallus of G. filicina was grown in axenic culture using both solid and liquid media. The various media tested were f/2, Provasoli’s Enriched Seawater (PES) and Enriched Seawater (ESW). The effect of glycerol (as a carbon source) and various plant growth regulators i.e. auxin (NAA) and cytokinins (Kinetin and BA) were tested. Although, regeneration of young thalli was observed from the cut ends in all the media, better growth was found in f/2, PES, f/2 (0.5% Glycerol), f/2 (NAA ${10^{-5}}_M)\;and\;f/2\;(BA\;{10^{-6}}_M$). On the other hand callusing was observed only in solid media supplemented with low concentration of Glycerol (0.5%) in f/2, NAA ${10^{-5}}_M\;in\;f/2,\;PES\;and\;BA\;{10^{-5}}_M$ in f/2. Young thalli were developed from the callus sub culture after 40 days of inoculation.

• 한국환경과학회지
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• 제29권11호
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• pp.1089-1098
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• 2020

To assess the trophic function of an artificial macroalgal habitat, we compared the stable carbon and nitrogen isotope ratios of two amphipods (Caprella sp. and Ampithoe sp.) and their potential food sources in a transplanted macroalgal habitat (Grateloupia asiatica) with those in a natural habitat. There were no significant differences in the isotopic values of both consumers and their potential food sources between the transplanted and natural habitats. Such isotopic similarities between the two sites indicate a comparable resource consumer relationship. Additionally, our results showed similar isotopic niche areas and high dietary overlap (>65%) of the two amphipods between the transplanted and natural sites, suggesting that the transplanted habitat plays ecological roles similar to the natural habitat. Overall, isotopic assessment can provide information on the trophic function of diverse transplanted macroalgal habitats and improve post-monitoring efforts in the management of artificial ecosystems.

• Journal of Microbiology and Biotechnology
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• 제24권3호
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• pp.346-353
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• 2014

The chlorophyll-related compound pheophorbide a (Pa) was successively purified from an edible red seaweed, Grateloupia elliptica, using silica, octadecyl silica column chromatography and reversed phase-high-performance liquid chromatography, as well as the cell cycle inhibitory and apoptotic effects of Pa being investigated in U87MG glioblastoma cells. The Pa exhibited strong anticancer effects in the absence of direct photo-irradiation against various cancer cell lines, including U87MG, SK-OV-3, and HeLa cells. Among the cancer cells, the strongest anticancer activity of Pa exhibited on U87MG cells with $IC_{50}$ values of 2.8 ${\mu}g/ml$. In addition, Pa specifically had cytostatic activity on glioblastoma cells rather than human umbilical vein endothelial cells. Analysis of the cell cycle distribution showed that Pa induced G0/G1 arrest of U87 MG cells. In addition, arrested cells induced late apoptosis and DNA degradation under dark condition. These results suggest that Pa isolated from G. elliptica is a potential glioblastoma-specific anticancer agent without side effects on normal cells.

• ALGAE
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• 제38권2호
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• pp.151-157
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• 2023

Grateloupia turuturu is a red alga with a flat but firm slippery thallus. Throughout its lifetime, this alga experiences a wide range of environmental stresses in the intertidal rocky shores. The aim of this study is to investigate the effect of salinity and irradiance on the early developmental stages of G. turuturu tetrasporophytes. The released carpospores were cultivated at different salinities (S = 15, 25, and 35) and irradiances (50, 100, and 200 μmol photons m^-2 s^-1). Germination of carpospores and development of juvenile tetrasporophytes were observed every 5 days and recorded by a digital camera. Discoid crusts were formed at all conditions within 5 days. The discoid crusts at 200 μmol photons m^-2 s^-1 died within 20 days regardless the salinity. The discoid crusts at S = 35 also died at all irradiance conditions within 25 days. Except for those at S = 35 and 200 μmol photons m^-2 s^-1, the discoid crusts reached about 8,000-9,000 ㎛² by day 20. Regardless of irradiance, the upright thalli formation rate from discoid crusts was 85 and 10% at S = 15 and S = 25, respectively. These results suggest that salinity and irradiance are important factors influencing early developmental stages of G. turuturu.

• 한국수산과학회지
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• 제9권3호
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• pp.163-168
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• 1976

부산시해운대연안에서 1975년 6월 23일 채취한 진두발, 지누아리 및 돌가사리의 3종류의 홍조류를 시료로 하여 이들 원조의 carrageenan함량과 이로부터 추출한 carrageenan의 $SO_4$ 및 3,6-anhydrogalactose의 함량, KCl 용액에 대한 용해도, KCl 논도별에 따라 분획한 획분의 3,6-anhydrogalactose의 함량에 대하여 조사검토하였다. 1. 원조별 carrageenan의 함량은 진두발이 가장 높고, 다음이 돌가사리, 지누아리의 순이었으며, 모두 $35\%$이상의 높은 함량을 보였다. 2. Carrageenan의 $SO_4$함량은 지누아리, 진두발, 돌가사리의 순으로 높았고, 3,6-anhydrogalactose의 함량은 반대로 돌가사리, 진두발, 지누아리의 순으로 높았다. 3. Carrageenan의 KCl 용액에 대한 용해도를 보면, 0.125 M KCl 농도에서 침전하는 비율은 진두발, 지누아리, 돌가사리의 Carrageenan 순으로 높았고, $0.125M\~2.0M$ KCl 농도에서 침전하는 비율은 돌가사리, 지누아리, 진두발의 carrageenan 순으로 높았으며, 2.0 M 이상의 KCl 농도에서도 용존하는 비율은 지누아리, 돌가사리, 진두발의 carrageenan 순으로 높았다. 4. Carrageenan을 0.0625 M와 0.25 M의 KCl 농도범위에서 3획분으로 분획하였을때, 각 획분의 수율은 진두발 및 돌가사리 carrageenan은 비슷한 경향을 보였고, 지누아리 carrageenan은 앞의 2종류에 비하여 가용획분의 수율이 많이 높았다. 5. 각획분의 $SO-4$ 및 3,6-anhydrogalactos 의 함량은 역상관관계를 나타내었고, KCl에 대한 용해도가 큰 획분일수록 $SO-4$ 함량이 높고 3, 6-angydrogalactose의 함량이 낮았다.

• 수산해양교육연구
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• 제27권6호
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• pp.1593-1601
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• 2015

Two marine algal halymeniacean species were collected from eastern coast of Korea during the survey of indigenous species of algae. One is distinct from other similar species by foliose thalli with irregular shape and several branches and linear proliferations without division. The other is characterized by indistinct midrib, foliose unbranched thalli and refractive ganglioid cells in medulla. These two species are identified as Grateloupia latissima and Cryptonemia yendoi based on the morphological features, respectively. Morphological and taxonomic notes on these species are included in the present study. This is the first record of G. latissima and C. yendoi from Korea.