• Title/Summary/Keyword: Gram-negative rod

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A Gram-negative halophilic carotenoid-producing bacterium, Paracoccus sp.

  • Lee, Jae-Hyung;Lee, Won-Jae;Kim, Young-Tae
    • 한국생물공학회:학술대회논문집
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    • 2003.04a
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    • pp.710-712
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    • 2003
  • A new species of Gram-negative halophilic cartenoid producing bacterium was isolated from the Haeundae Coast, Korea. This strain is non-motile, aerobic, orange-pigmented, rod-shaped, and produced carotenoids, mainly astaxanthin. All the type strains of the genus Paracoccus were compared with this strain using 16S rDNA sequence analysis, fatty acid patterns, and physiological reaction profiles. From the results obtained, this strain is classified as a new species, Paracoccus sp..

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Isolation and Identification of High Phosphate-accumulating Bacteria (인 축정능이 우수한 세균의 분리 및 동정)

  • 신공식;고정연;최우영
    • Microbiology and Biotechnology Letters
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    • v.27 no.4
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    • pp.286-291
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    • 1999
  • By using autoradiography and phosphate medium, high phosphate accumulating bacteria were isolated from the soil of protected cultivation area and activated sludge. Selected strain, PO8, was gram-negative, rod/spherial(0.5~0.6$\times$1.0~1.2${\mu}{\textrm}{m}$ in size) and non-motile. PI4, another selected strain, was gram-negative, rod(0.4~0.5$\times$1.5~1.6${\mu}{\textrm}{m}$ in size) and motile. It also had flagella. According to their morphological, physiological and biochemical properties, the stains were identified as Acinetobacter lwoffi PO8 and Chromobacterium lividum PI4, respectively. A. lowoffi PO8 and C. lividum PI4 cultured in the P-1 medium containing 150ppm phosphate were able to uptake high phosphate up to 92% and 85%, respectively after 24 hours at 3$0^{\circ}C$ during liquid culture.

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Isolation and Characterization of Six Microorganisms from the Digestive Tract of the Cricket Gryllus bimaculatus (쌍별귀뚜라미(Gryllus bimaculatus) 소화기관에서 분리한 6종류의 특성규명)

  • Kwon, Kisang;Lee, Eun Ryeong;Yoo, Bo-Kyung;Ko, Young Hwa;Shin, Hyojung;Choi, Ji-Young;Kwon, O-Yu
    • Journal of Life Science
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    • v.27 no.9
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    • pp.1040-1046
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    • 2017
  • We describe the isolation and characterization of six different intestinal microorganisms from the digestive tract of the cricket Gryllus bimaculatus. Based on 16S rRNA gene sequences, we obtained six isolates belonging to four different genera: Staphylococcus, Bacillus, Citrobacter, and Proteus. All the isolates were resistant to ampicillin. Ampicillin is an irreversible inhibitor of the enzymeetranspeptidase, which is needed to make bacterial cell walls. None of the isolates were resistant to kanamycin, which binds to the 30S subunit of the bacterial ribosome and then inhibits total protein synthesis. Gram staining was conducted, in addition to morphological classification under a microscope. Four grampositive isolates and two gram-negative isolates were detected. The gram-positive isolates were GL1 (round shaped, 2 am in diameter), GL2 (rod shaped, $2.5{\mu}m$ in length), GL3 (rod shaped, $2{\mu}m$ in length), and GL4 (round shaped, $1.5{\mu}m$ in diameter). The gram-negative isolates were GL5 (rod shaped, $2{\mu}m$ in length) and GL6 (rod-shaped, $2.5{\mu}m$ in length). Notably, two of the isolates, GL2 and GL4, secreted specific extracellular proteins. These were determined by MALDI-TOF-MS spectral analysis to be a 87 kDa collagenase, 56 kDa hypothetical protein, and 200 kDa hypothetical protein. The six isolates in this study could be used for various biotechnological applications and pest management, both in the field and in greenhouse systems. In addition, it would be interesting to determine the relationship between these isolates and their host.

Distribution of saprophytic bacteria and correlation-coefficient analysis in the seawater of Chungmu bay, Korea. (忠武灣 海水의 腐生細菌 分布와 相關係數 分析에 關하여)

  • Hong, Soon-Woo;Hah, Yung-Chil;Ahn, Tae-Young
    • 한국해양학회지
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    • v.20 no.3
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    • pp.62-69
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    • 1985
  • Comparision of the classical taxonomy and the numerical taxonomy was carried out on 108 strains isolated from Chung-mu bay and it was confirmed that correlation coefficient measured between strains represented similarities. Gram stain is the most important test in the classical taxonomy, while Gram positive and Gram negative strains were distributed evenly over the whole cluster and coccus and rod were separated in the numerical taxonomy. Both the numerical taxonomy and the classical taxonomy showed high similarity in the classification between the genera. The strains collected in bottom layer marked lower taxonomic similarity between strains than those in upper and middle strains. The distance between the inside (Group I) and the outside (Group II) strains of the bay was larger than that between the strains collected in high and low tide.

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A report of six unrecorded radiation-resistant bacterial species isolated from soil in Korea in 2018

  • Maeng, Soohyun;Sathiyaraj, Srinivasan;Subramani, Gayathri;Kim, Ju-Young;Jang, Jun Hwee;Kang, Myung-Suk;Lee, Ki-Eun;Lee, Eun-young;Kim, Myung Kyum
    • Journal of Species Research
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    • v.7 no.3
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    • pp.222-230
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    • 2018
  • Six bacterial strains 18JY42-3, 18SH, 18JY76-11, 17J11-11, 18JY14-14, and 18JY15-11 assigned to the phylum Proteobacteria, Firmicutes, and Actinobacteria were isolated from soil samples in Korea. The Cohnella species, strain 18JY42-3 was Gram-stain-positive, short rod-shaped and beige-colored. The Methylobacterium species, strains 18SH and 18JY76-11 were Gram-stain-negative, short rod-shaped and pink-colored. The Microterricola species, strain 17J11-11 was Gram-stain-positive, short rod-shaped and yellow-colored. The Paenarthrobacter species, strains 18JY14-14 and 18JY15-11 were Gram-stain-positive, short rod-shaped and white-colored. Phylogenetic analysis based on 16S rRNA gene sequence showed that strains 18JY42-3, 18SH, 18JY76-11, 17J11-11, 18JY14-14, and 18JY15-11 were most closely related Cohnella rhizosphaerae (MH497628; 98.8%), Methylobacterium goesingense (MH497632; 99.1%), Methylobacterium populi (MH497635; 99.9%), Microterricolagilva (MH504108; 98.4%), Paenarthrobacter nicotinovorans (MH497641; 100%), and Paenarthrobacter nitroguajacolicus (MH497646; 99.2%), respectively. All the six unrecorded strains showed resistance to UV radiation. This is the first report of these six species in Korea.

Wild Raccoons (Procyon lotor) with Ancylostomiasis in Kora (한국 야생 너구리에서 발생한 구충증)

  • 조경오;박남용;강성귀;김용환;배성열;김영대;김계엽;이근우
    • Journal of Veterinary Clinics
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    • v.18 no.2
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    • pp.178-181
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    • 2001
  • Four dead wild racoons found in Chonnam province were examined parasitologically, pathologically and bacteriologically. Using standard flotation technique with feces many number of ancylostoma eggs which were oval in shape and $60-70{\times}35-45 {\mu}m$ in size were detected. Grossly, severe anemia, multiple congestion and consolidation of lung and catarrhal exudate in small intestine were observed. One of racoons had multifocal white to yellow nodules in the liver. Histopathologically, ancylostoma larvas were found in intestinal lumen. Some of them penetrated and attached to intestinal mucosa. Lungs revealed multiple severe fibrinopurulent pneumonia in which bacterial colonies were scattered. Liver had multiple microabscesses containing peripheral bacterial colony. By Gram stain using paraffin sections of liver and lung, bacterial colony was Gram negative. Isolated bacteria from lung and liver was Gram negative rod. This bacteria was identical with Escherichia coli by biochemical tests. From these results, ancylostomiasis caused severe problems in Korean wild racoons. This is the first report about ancylostomiasis in wild racoons in Korea.

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Isolation and Identification of Pathogenic Microorganisms from Soybean Sprouts

  • Kim, Hye-Jung;Koo, Kyoung-Mo;Kim, Gi-Nahm;Lee, Dong-Sun;Paik, Hyun-Dong
    • Preventive Nutrition and Food Science
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    • v.7 no.3
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    • pp.305-309
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    • 2002
  • Raw soybean sprouts were tested for contamination with the following bacteria which have potential for pathogenesis or food spoilage : Salmonella spp., Escherichia coli O157:H7, Yersinia enterocolitica, Vibrio parahae-molyticus, Aeromonas hydrophila, Plesidomonas shigeloides, Pseudomonas aeruginosa, Staphylococcus aureus, Lis-teria monocytogenes, Bacillus cereus, Clostridium perfringens, Campylobacter jejuni, Erwinia spp., and Fusarium spp. Three of the above strains were isolated from the sprouts, and identified by morphological and biochemical methods including an API kit and ATB automated identification system. The isolate cultured in Cereus selective agar, a selective medium, was a Gram-positive, rod shaped, anaerobic spore former. The biochemical and culture tests revealed the following characteristics: catalase-positive, no growth on Simmon's citrate, NO₂ production and requirement of arginine for growth; the ATB automated identification system gave 99.8 % agreement for the identification of Bacillus cereus to the species level. The isolate cultured in Macconkey agar selective medium was Gram-negative, rod shaped and a gas former; the ATB-system gave 99.9% agreement for the identification of Aeromonas hydrophila to the species level. The isolate found in Pseudomonas isolation agar was Gram-negative, rod shaped, cytochrome oxidase-positive, a reducer of nitrates to nitrogen, and pyocyanin producer; the ATB-system gave 99.9 % agreement for the identification of Pseudomonas aeruginosa to the species level. These results indicate that the three bacteria species present in the soybean sprouts were Bacillus cereus, Aero-monas hydrophila, and Pseudomonas aeruginosa. Salmonella spp., Escherichia coli O157:H7, and Yersinia enter-ocolitica, which are associated with serious disease in humans, were not isolated from soybean sprouts examined in this study.

The Biochemical Characteristics of Acetobacter sp. Isolated from Persimmen Vinegar (감식초에서 분리한 Acetobacter sp.의 생화학적 특성)

  • 이상복;전승호
    • Journal of environmental and Sanitary engineering
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    • v.14 no.4
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    • pp.180-187
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    • 1999
  • The following is experimental result of Biochemical characteristics of Acetobacter sp. Acetobacter sp. were gram negative, short rod, non-spore-forming and motile. It reacted positively catalase, methyl red, oxidation fementation, voges-proskauer and nitrate reduction tests and hydrogen sulfide test negative and ONPG negative. Acetobacter sp. showed normal growth curve in Carr broth and there was no significant difference between isolates and type strains such as Acetobacter aceti(KCTC 1010), Acetobacter diazotrophicus(KCTC 2859). Acetobacter liquefaciens(KCTC 2804) and Acetobacter pasteurianus(KCTC 1008).

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Microbial Quality and TBA Values of Chicken Patties as Affected by Irradiation and Storage Temperature (방사선 조사에 의한 닭고기 가공제품인 Patty의 미생물 및 TBA가 변화)

  • Chuang, J.T.;Yi, Y.H.;Chen, T.C.
    • Korean Journal of Food Science and Technology
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    • v.22 no.3
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    • pp.290-295
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    • 1990
  • prefried chicken patties were irradiated with doses of 0, 2, and 4kGy, and stored at $3{\pm}1^{\circ}C\;and\;-10{\pm}1^{\circ}C$, separately for the evaluation of microbiological quality and TBA values. Gamma irradiation increased (p<0.05) the shelf life of chicken patties stored at $3{\pm}1^{\circ}C$. The time required to reach a log number of 6.5/g was 22 days for the control, while 43 and 44 days for 2 and 4 kGy-irradiated samples, respectively. No microorganism was isolated from patties irradiated at 4 kGy. Irradiation decreased the incidence of gram-positive cocci-type organisms In patties ; while yeasts and gram-negative rod-type organisms dominated the microbial population isolated from 2 and 4 kGy-irradiated samples, respectively. TBA values were increased (p<0.05) as the irradiation dose increased and the storage time progressed.

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반혐기성 가수분해/산 발효조에서의 음식폐기물 발효 균주 탐색

  • Kim, Jung-Gon;Kim, Si-Uk
    • 한국생물공학회:학술대회논문집
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    • 2002.04a
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    • pp.427-430
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    • 2002
  • Pilot scale(2.5 ton) three-stage methane fermentation process was developed for the rapid production of methane from food wastes in our laboratory. Eleven strains responsible for the primary semianaerobic hydrolysis/acidogenic fermentation system were isolated and characterized. Among them, the number of gram positive bacteria was eight and that of gram negative bacteria was three. They were rod and showed positive reaction to catalase. The strain K5 was found to have the highest enzyme activities of amylase and protease.

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