Isolation and Identification of High Phosphate-accumulating Bacteria

인 축정능이 우수한 세균의 분리 및 동정

  • 신공식 (충북대학교 원예학과 첨단원예기술개발연구센터) ;
  • 고정연 (충남대학교 농화학과) ;
  • 최우영 (충남대학교 농화학과)
  • Published : 1999.08.01

Abstract

By using autoradiography and phosphate medium, high phosphate accumulating bacteria were isolated from the soil of protected cultivation area and activated sludge. Selected strain, PO8, was gram-negative, rod/spherial(0.5~0.6$\times$1.0~1.2${\mu}{\textrm}{m}$ in size) and non-motile. PI4, another selected strain, was gram-negative, rod(0.4~0.5$\times$1.5~1.6${\mu}{\textrm}{m}$ in size) and motile. It also had flagella. According to their morphological, physiological and biochemical properties, the stains were identified as Acinetobacter lwoffi PO8 and Chromobacterium lividum PI4, respectively. A. lowoffi PO8 and C. lividum PI4 cultured in the P-1 medium containing 150ppm phosphate were able to uptake high phosphate up to 92% and 85%, respectively after 24 hours at 3$0^{\circ}C$ during liquid culture.

Keywords

References

  1. Standard Methods for the Examination of Water and Wastewater(19th ed.) Callaway, J. O.
  2. J. Microbiol. v.54 Role of cations in accumulation and release of phosphate by Acinetobacter strain 210A Groenestijn, J. W.;G. J. F. M. Vlekkle;C. M. E. Anink;M. H. Deinema;A. J. B. Zehnder
  3. J. Bacteriol. v.86 Accumulation of inorganic polyphosbate in Aerobacter aerogenes Harold, F. M.
  4. Bacteriol. Rev. v.30 Inorganic polyphosphates in biology structure, metabolism, and function Harold, F. M.
  5. J. Ferment. Technol. v.62 Differences in phototrophic growth on high phosphate concentrations among Rhodopseudomonas species Hiraishi, A.;H. Kitamura
  6. J .Ferment. Bioeng. v.69 Capacity for polyphosphate accumulation of predominant bacteria in activated sludge showing enhanced phosphate removal Hiraishi, A.;Y. Morishima
  7. Appl. Environ. Microbiol. v.59 Genetic improvement of Escherichia coli for enhanced biological removal of phosphate form wastewater Kato, J.;K. Yamada;A. Muramatsu;H. Ohtake
  8. Methods in Microbiology Lipid and cell-wall analysis in bacterial systematics Komagata, K.;K. Suzuki
  9. Bergey's Manual of Systematic Bacteriology Krieg, N. R.;J. G. Holt
  10. Anal. Chem. v.31 Use of dinitrosalicylic acid reagent for determination of reducing sugar Miller, G. L.
  11. Appl. Microbiol. Biotechnol. v.24 The effect of acetate and succinate on polyphosphate formation and degradation in activated sludge, with particular reference to Acinetobacter calcoaceticus Murphy, M.;L. H. Lotter
  12. J. Ferment. Bioeng. v.71 Isolation of a new type of polyphosphate accumulating bacterium and its phosphate removal characteristics Nakamura, K.;K. Masuda;E. Mikami
  13. J. Ferment. Bioeng. v.80 Phosphate uptake and release activity in immobilized poly-phosphate accumulating bacterium Microlunatus phosphovorus strain NM-1 Nakamura, K.;S. Ishikawa;M. Kawaharasaki
  14. Wat. Res. v.19 Uptake and release of phosphate by a pure culture of Acinetobacter calcoaceticus Ohtaka, H.;K. Takahashi;Y. Tsuzuki;K. Toda
  15. J. Bacteriol. v.162 Amount and chain length of polyhosphate in Escherichia coli depend on cell growth conditions Rao, N. N.;M. F. Roberts.;A. Torriani
  16. Agric. Biol. Chem. v.44 Screening for high phosphate accumulating bacteria Shoda, M.;T. Ohsumi;S. Udaka
  17. Ph D. Dissertation, Chonnam National University Biological recycling of the insoluble phosphates accumulated in cultivated soils by the phosphate-solu-bilzing microorganisms Suh, S. S.
  18. Wat. Sci. Tech. v.17 Polyphosphate kinase activity during phosphate uptake by bacteria T'Seyen, J.;D. Malnou;J. C. Block;G. Faup
  19. Wat. Sci. Tech. v.21 The role of acetate in denitrification and biological phosphate removal in modified bardenpho systems Winter, C. T.
  20. Appl. Environg. Microbiol. v.20 Biological uptake of phosphorus by activated sludge Yall, I.;W. H. Boughton;R. C. Knudsen;N. A. Sinclair
  21. J. Ferment. Technol. v.66 Phosphorus removal by pure and mixed cultures of microorganisms Ye, Q.;H. Ohtake;K. Toda