• 한국동물번식학회:학술대회논문집
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• 한국동물번식학회 2004년도 춘계학술발표대회
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• pp.227-227
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• 2004

The purpose of this study was to investigate the effect of taurine on sperm characteristics and gene expressions(bax and Gpx) in fresh boar semen during in vitro storage. The motility of spermatozoa in Modena, Modana plus taurine 25 mM, Modana plus taurine 50 mM, Modana plus taurine 75 mM and Modana plus taurine 100 mM were 63.1%, 65.1%, 65.3%, 82.5% and 80.8%, respectively. (omitted)

• 한국가금학회지
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• 제50권2호
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• pp.109-118
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• 2023

본 연구는 고온기 복합생균제(L. plantarum, B. subtilis, Saccharomyces cerevisiae) 급여가 육계의 항산화 방어작용에 미치는 영향을 조사하기 위하여 대조군(CON), 0.25% 생균제 급여군(LPB) 및 0.5% 생균제 급여군(HPB) 등 3 처리군으로 설정하여 혈장, 소장 점막 및 간 조직에서 항산화 지표를 분석하였다. 실험 결과, 혈장 total protein, albumin, bloodurea nitrogen(BUN) 및 glutathione(GSH) 수준 등 질소화합물은 처리군 간 차이가 없었다. 혈장에서 조사한 항산화 효소 superoxide dismutase(SOD), glutathione peroxidase(GPX)와 glutathione S-transferase(GST) 및 지질과산화(MDA) 역시 생균제 급여에 따른 유의적 차이가 없었다. 소장점막조직에서 SOD 활성도는 0.5% 생균제 급여군(HPB)에서 대조군과 LPB군 보다 유의하게(P<0.05) 증가하였다. 소장 점막조직의 MDA 수준은 대조군보다 HPB군에서 현저하게(P<0.05) 감소하였다. 그러나 소장 점막조직의 GPX와 GST 활성도는 생균제 급여에 따른 차이는 없었다. 간 조직에 존재하는 SOD, GPX, GST 활성도 및 MDA 수준은 생균제 급여와 급여 수준에 따른 영향을 받지 않았고 모든 군에서 비슷한 수준을 보였다. 따라서 HPB군의 소장 점막조직에서 SOD 활성도가 증가하고 지질과산화도가 감소하는 결과로 보아 고온기에 0.5% 생균제 급여는 육계 소장 점막조직의 항산화 방어작용에 긍정적인 효과를 미치는 것으로 나타났다.

• 한국식품위생안전성학회지
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• 제26권2호
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• pp.166-170
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• 2011

pH와 DO(dissolved oxygen)의 변화는 발효 진행상황을 파악하는데 중요한 요인이다. 일반적으로 효모의 알코올 발효는 술덧 (mash)이 산성 또는 미산성인 경우에 알코올 생성 능력이 좋은 것으로 알려져 있다. 산성막걸리의 1차 담금시 pH는 3.20에서 시작하여 발효가 진행됨에 따라 4.22로 증가하였다. 보통 1차 담금 후 2시간이 경과하였을 때 pH는 3.2정도가 되면 정상발효를 하는 것으로 알려져 있다. 72시간 후 2차 담금 시에는 pH가 3.88에서 3.67사이로 확인 하였다. 일반 막걸리의 발효 pH는 4.0~4.6범위인데 본 연구에서는 다소 낮은 pH를 나타내었다. 또 숙성단계에서는 3.72 에서 4.11로 증가하여 산성의 pH를 유지하는 것을 확인하였다. DO의 경우 l차 담금 시 10에서 시작하여 0.8정도로 감소하다가 2차 담금과 숙성 시에는 4.0을 유지하였다. SOD는 superoxide anion을 $H_2O_2$와 $O_2$로 전환시키고 $H_2O_2$는 다시 GPx와 catalase(CAT)에 의해 $H_2O$로 전환됨으로써 SOD, CAT 및 GPx는 활성산소의 독성으로부터 생체를 보호하는데 매우 중요한 역할을 하는 효소이다. SOD 활성도는 LPS를 투여한 대조군이 정상군에 비하여 1.64배 감소하였으며 SM+LPS군의 SOD 활성도는 대조군에 비해 23.29% 증가하여 간 염증이 억제되었음을 확인하였다. LPS로 간 염증을 유발하지 않은 SM군에서는 정상군보다 1.28배 SOD활성이 증가하였다. CAT 활성도에서는 대조군이 정상군에 비해 l.35배 CAT의 활성도를 낮춘 것으로 나타났다. SM+LPS 군의 CAT 활성도는 대조군에 비하여 40.26% 증가함을 확인하였다. SM군은 정상군에 비해 1.03배 CAT의 활성이 증가하였다. GPx활성도는 대조군이 정상군에 비하여 3.78배 감소하였으며 SM+LPS군의 GPx활성도는 대조군에 비하여 11.91% 증가함을 확인하였다. SM군은 GPx활성이 정상군에 비해 0.97배 증가하였다. 따라서 본 연구에서는 항산화 효소의 활성이 LPS투여로 감소되었으나 SM을 투여한 경우에는 증가하였음을 관찰할 수 있었다. 항산화 효소의 활성의 증가는 간 손상에 관한 간조직의 병리조직학적 관찰을 통하여 입증되었다. 그러므로 산성막걸리 추출물이 항산화 작용에서 효과가 있는 물질로 판단된다.

• 대한방사선기술학회지:방사선기술과학
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• 제40권1호
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• pp.135-142
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• 2017

천연물에 존재하는 셀레늄(Se)은 생체의 산화환원작용을 주관하는 중요한 단백질의 하나인 셀레늄함유단백질(selenoprotein)의 중요한 요소로 알려져 있다. 셀레늄(Se)을 Rat에 경구 투여하여 10 Gy의 방사선을 조사 시킨 후 갑상선을 표적 장기로 삼고 1일, 7일, 21일 기간에 따른 혈구성분의 변화, 갑상선호르몬(T3, T4)의 변화, 항산화효소((Glutathione Peroxidase, GPx)의 활성 변화, 갑상선 조직 변화 관찰을 통하여 셀레늄(Se)의 방사선 방호 작용을 알아보고자 하였다. 실험결과 조혈 면역계(혈색소농도, 호중구, 혈소판)에서 회복을 보이는 유의한 방호 효과가 있었다(p<0.05). 항산화효소인 Glutathione Peroxidase(GPx) 활성과 표적 장기인 갑상선 호르몬(T3, T4)의 활성 변화 결과에서도 유의성 있는 활성 변화를 보였으며(p<0.05), 조직 변화 관찰에서는 방사선 처리에 의한 세포 괴사를 일으킨 갑상선 세포 손상 보호 효과가 있음을 확인하였다. 따라서, 셀레늄(Se)은 떨어진 생체의 면역 활성 효과를 유도함으로써 방사선 방어제로 활용될 수 있을 것이라 판단된다.

• Biomolecules & Therapeutics
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• 제9권4호
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• pp.249-257
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• 2001

The effect of oxidative stress on the alterations of different antioxidant enzyme activities was investigated in human skin melanoma cell line (SK-MEL-2). Oxidative stress was induced by the exposure to hydrogen peroxide ($H_2O$$_2$). SK-MEL-2 cells were treated with antioxidants such as vitamin E and selenomethionine in combination with $H_2O$$_2$. SK-MEL-$_2$ cells were exposed to various concentrations of $H_2O$$_2$ and measured the time course of changes in cell viability and antioxidant enzyme activities for 24 hr. Oxidative stress was induced by the exposure to 2.5mM hydrogen peroxide ($H_2O$$_2$) resulted in declining significantly for 24 hr. GPX and CAT activities peaked at 3 hr and returned to control levels by 24 hr. On the contrary, SOD activity was inactive before 6 hr but recovered at 24 hr. In case vitamin E (Vit E) and selenomethionine (Se-Met) were used at nontoxic concentrations (25$\mu$M Vit E/500$\mu$M Se-Met) to oxidative stress was induced by the exposure to hydrogen peroxide ($H_2O$$_2$) led to a 3- and 5-fold increase on the viability comparing to control and caused an increase in GPX activity respectively.

• 한국축산식품학회지
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• 제35권6호
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• pp.847-858
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• 2015

The study aimed to evaluate the effect of kefir combination from goat milk and soy milk on lipid profile, plasma glucose, glutathione peroxidase (GPx) activity and the improvement of pancreatic β-cell in diabetic rats. Male rats were divided into five treatments: normal control, diabetic control, goat milk kefir, combination of goat milk-soy milk kefir and soy milk kefir. All rats were induced by streptooztocin-nicotinamide (STZ-NA), except for normal control. After 35 d experiment, the rats were sampled for blood, sacrificed and sampled for pancreatic tissues. Results showed that diabetic rats fed kefir combination had higher (p<0.05) triglyceride than the rats fed goat milk or soy milk kefir. Decreasing of plasma glucose in diabetic rats fed kefir combination was higher (p<0.05) than rats fed goat millk kefir. The activity of GPx in diabetic rats fed three kinds of kefir were higher (p<0.01) than untreated diabetic rats. The average number of Langerhans and β-cells in diabetic rats fed kefir combination was the same as the normal control, but it was higher than diabetic control. It was concluded that kefir combination can be used as antidiabetic through maintaining in serum triglyceride, decreasing in plasma glucose, increasing in GPx activity and improving in pancreatic β-cells.

• 약학회지
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• 제46권1호
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• pp.39-46
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• 2002

The water fraction exhibiting anticancer activity was prepared from 70% methanol extract of Artemisis argyi by stepwise solvent partioning. This water fraction(5 $\mu$g/ml concentration) showed a considerable cytotoxicity against leukemic L1210 cells with a maximal value of 92% for 3 days culture. Contrastingly to such substantial anticancer activities the identical fraction showed far low toxicity against normal lymphocytes than chloroform fraction of Artemisia argyi mitomycine and 5-fluorouracil at every concentration ranging 0.01$\mu$g/ml~10.00$\mu$g/ml. The cytotoxicity displayed against L1210 cells by the water fraction of Artemisia was found to be proportinal to the decrease of viability of L1210 cells. On the other hand, $O_2$ion generation in L1210 cells appeared to be elevated in accordance to cytotoxicity by the water fraction with concurrent increases of superoxide dismuatse (SOD) and glutathione peroxidase (GPx) which are responsible for the conversion of $O_2$ ion and $H_2O$$_2$ respectively These findings taken together indicate that the death of L1210 cells by the water fraction of Auemisia atgyi, may be induced at least in part by the detrimental action of reactive oxygen species (ROS) including $O_2$- in spite of substantial extorts of SOD and GPx to overcome the attack of ROS.

• 약학회지
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• 제48권1호
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• pp.93-98
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• 2004

The purpose of this study was to evaluate the effect of pharbitidis seed extract (PE) on antioxidant enzymes. The cytotoxicities of PE were measured by 3- (4,5-dimethlthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay; The change of superoxide dismutase (SOD), glutathione peroxidase (GPx), and catalase (CAT) activity assay were measured. The SOD activities by PE-treated groups were lower than control group's one. In the co-treated with hydrogen peroxide ($H_2O$$_2$) group, SOD activity was higher than $H_2O$$_2$ treated group's activity In the case of GPx, GPx activities were increased in both PE-treated and co-treated with $H_2O$$_2$ group. In the case of CAT $H_2O$$_2$ treated group's activityies were very increased. The CAT activities by PE-treated groups were lower than control group's one, but the activity of co-treated group with H $_2$O$_2$ was higher than that of control group's one. These results suggest that PE has antioxidant activity.

• Journal of Photoscience
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• 제9권2호
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• pp.430-432
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• 2002

The changes in expression of copper-zinc superoxide dismutase (CuZn-SOD), manganese superoxide dismutase (Mn-SOD) and glutathione peroxidase (GPX) in light-damaged rat retinas were examined. Sprague-Dawley rats (male, 6-weeks-old) were maintained on a cyclic photoperiod (12 hours light and 12 hours darkness) for 2 weeks. The illumination intensity during the light period was 80 lux. To induce light damage to the retina, a high-intensity illumination (3000-lux) was applied to the animals for 24 hours. After light exposure, the animals were returned to cyclic lighting. Eyes were enucleated 12 and 24 hours after light exposure started or 1,3, and 7 days after light exposure ended. Eyes were fixed and embedded in paraffin wax. Tissues were cut into 4${\mu}{\textrm}{m}$-thick sections. Sections were immunostained using antibody against CuZn-SOD, Mn-SOD, GPX and 8-hydroxy-deoxyguanocine (8-OHdG) as oxidative stress marker. 8-OHdG was observed in the outer nuclear layer (ONL) and retinal pigment epithelium (RPE) during light exposure. In light-damaged retinas CuZn-SOD labeling was up regulated in the ONL and RPE. Mn-SOD labeling was up regulated in rod inner segments (RIS) during light exposure and that in the RPE was up regulated after exposure. GPX labeling was observed in rod outer segments (ROS) during light exposure. GPX labeling was also observed in the RPE during and after light exposure. All three enzymes were observed in the outer retina, which suffered light damage, but occurred in defferent layers except within the RPE, in which case all three were expressed. These enzymes may play complementary roles as protective factors in light-damaged retinas.

• Journal of Microbiology and Biotechnology
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• 제24권5호
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• pp.605-613
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• 2014

We have previously shown that paraquat (PQ)-induced oxidative stress causes dramatic damage in various human cell lines. Naringenin (NG) is an active flavanone, which has been reported to have beneficial bioactivities, including antioxidative, anti-inflammatory, and antitumorigenic activities, with a relatively low toxicity to normal cells. In this study, we intended to assess the cytoprotective effect of NG against PQ-induced toxicity in the human bronchial epithelial BEAS-2B cell line. Co-treatment with NG in PQ-treated BEAS-2B cells can reduce PQ-induced cellular toxicity. NG can also decrease the generation of intracellular ROS caused by PQ treatment. We also observed that treatment with NG in PQ-exposed BEAS-2B cells can significantly induce the expression of antioxidant-related genes, including GPX2, GPX3, GPX5, and GPX7. NG co-treatment can also activate the NRF2 transcription factor and promote its nuclear translocation. In addition, NG co-treatment can induce the expression of NRF2-downstream target genes such as that of heme oxygenase-1 (HO-1) and NAD(P)H:quinone oxidoreductase 1 (NQO1). A small interfering RNA study revealed that the knockdown of NRF2 can abrogate NG-mediated protection of the cells from PQ-induced cellular toxicity. We propose that NG effectively alleviates PQ-induced cytotoxicity in human bronchial epithelial BEAS-2B cells through the NRF2-regulated antioxidant defense pathway, and NG might be a good therapeutic candidate molecule in oxidative stress-related diseases.