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Effect of Pharbitidis Seed Extract on the Antioxidant Enzyme Activity in B16F10 Murine Melanoma Cells by Oxidative Stress  

김안근 (숙명여자대학교 약학대학)
차은정 (숙명여자대학교 약학대학)
Publication Information
YAKHAK HOEJI / v.48, no.1, 2004 , pp. 93-98 More about this Journal
Abstract
The purpose of this study was to evaluate the effect of pharbitidis seed extract (PE) on antioxidant enzymes. The cytotoxicities of PE were measured by 3- (4,5-dimethlthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay; The change of superoxide dismutase (SOD), glutathione peroxidase (GPx), and catalase (CAT) activity assay were measured. The SOD activities by PE-treated groups were lower than control group's one. In the co-treated with hydrogen peroxide ($H_2O$$_2$) group, SOD activity was higher than $H_2O$$_2$ treated group's activity In the case of GPx, GPx activities were increased in both PE-treated and co-treated with $H_2O$$_2$ group. In the case of CAT $H_2O$$_2$ treated group's activityies were very increased. The CAT activities by PE-treated groups were lower than control group's one, but the activity of co-treated group with H $_2$O$_2$ was higher than that of control group's one. These results suggest that PE has antioxidant activity.
Keywords
antioxidant enzyme activity; pharbitidis seed; ROS;
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