산화적 스트레스상태에서 B16F10 Murine Melanoma 세포의 항산화효소 활성에 대한 흑축추출물의 효과

Effect of Pharbitidis Seed Extract on the Antioxidant Enzyme Activity in B16F10 Murine Melanoma Cells by Oxidative Stress

  • 김안근 (숙명여자대학교 약학대학) ;
  • 차은정 (숙명여자대학교 약학대학)
  • 발행 : 2004.02.01

초록

The purpose of this study was to evaluate the effect of pharbitidis seed extract (PE) on antioxidant enzymes. The cytotoxicities of PE were measured by 3- (4,5-dimethlthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay; The change of superoxide dismutase (SOD), glutathione peroxidase (GPx), and catalase (CAT) activity assay were measured. The SOD activities by PE-treated groups were lower than control group's one. In the co-treated with hydrogen peroxide ($H_2O$$_2$) group, SOD activity was higher than $H_2O$$_2$ treated group's activity In the case of GPx, GPx activities were increased in both PE-treated and co-treated with $H_2O$$_2$ group. In the case of CAT $H_2O$$_2$ treated group's activityies were very increased. The CAT activities by PE-treated groups were lower than control group's one, but the activity of co-treated group with H $_2$O$_2$ was higher than that of control group's one. These results suggest that PE has antioxidant activity.

키워드

참고문헌

  1. Free Radical Biol. Med. v.19 Antioxidant enzyme levels as a function of growth state in cell culture Terry D. Oberley;Janice L. Schultz;Ning Li;Larry W. Oberley https://doi.org/10.1016/0891-5849(95)00012-M
  2. J. Dermatol Sci. v.14 Time course of changes in antioxidant enzymes in human skin fibroblasts after UV irradiation Yasuko, Shindo;Takashi, Hashimoto https://doi.org/10.1016/S0923-1811(96)00578-6
  3. J. Am. Acad. Dermatol. v.38 Protective effect against sunburn of combined systemic ascorbic acid (vitamin C) and a-tocopherol (vitamin E) Bernadette,E.K.;Marianne,D.;Bernhard,P. https://doi.org/10.1016/S0190-9622(98)70537-7
  4. Casarett and Doull's toxicology Mechanisms of toxicity Zoltan, Gregus;Curtis D. Klaassen
  5. Casarett and Doull's toxicology Toxic responses of the heart and vascular systems Kenneth S. Ranos;Enrique Chacon;Jr.Daniel Acosta
  6. British Journal of Nutrition v.84 The effect of vitamin C or vitamin E supplementation on basal and H₂O₂-induced DNA damage in human lymphocytes Lisa A. Brennan;Gerard M. Morris;Gillian R. Wasson;Bernadette M. Hannigan;Yvonne A. Barnett https://doi.org/10.1079/096582197388680
  7. Rev. Med. Chir. Soc. Med. Nat. Iasi. v.103 Oxidative stress and anti-oxidants in the diet in pathological processes at the level of the cardiovascular system Gavat,V.;Voroniuc,O.
  8. Free Radical Biol. Med. v.24 Alterations of anti-oxidant enzyme expression in response to hydrogen peroxide Elke, Rohrdanz;Regine, Kahl https://doi.org/10.1016/S0891-5849(97)00159-7
  9. Eur. J. Biochem. v.47 Involvement of the superoxide anion radical in the autoxidation of pyrogallol and a convenient assay for superoxide dismutase Stefan, Marklund;Gudrun, Marklund https://doi.org/10.1111/j.1432-1033.1974.tb03714.x
  10. Anal. Biochem. v.137 The pyrogallol assay for superoxide dismutase: absence in glutathione artifact Eugene F. Roth,Jr.;Harriet S. Gilbert https://doi.org/10.1016/0003-2697(84)90344-0
  11. Methods in Enzymology v.105 Assays of glutathione peroxidase Flohe, Leopold;Wolfgang A. Gunzler https://doi.org/10.1016/S0076-6879(84)05013-8
  12. J. Lab. & Clin. Med. v.70 Studies on the qauntitative and qualitative characterization of erythrocyte glutathione peroxidase Donald E. Paglia;William N. Valentine
  13. Methods in Enzymology v.105 Catalase in vitro Hugo, Abei https://doi.org/10.1016/S0076-6879(84)05013-8