• Title/Summary/Keyword: Gossypium arboreum

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Diversity in Betasatellites Associated with Cotton Leaf Curl Disease During Source-To-Sink Movement Through a Resistant Host

  • Khan, Iftikhar Ali;Akhtar, Khalid Pervaiz;Akbar, Fazal;Hassan, Ishtiaq;Amin, Imran;Saeed, Muhammad;Mansoor, Shahid
    • The Plant Pathology Journal
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    • v.32 no.1
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    • pp.47-52
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    • 2016
  • Cotton leaf curl is devastating disease of cotton characterized by leaf curling, vein darkening and enations. The disease symptoms are induced by DNA satellite known as Cotton leaf curl Multan betasatellite (CLCuMuB), dominant betasatellite in cotton but another betasatellite known as Chili leaf curl betasatellite (ChLCB) is also found associated with the disease. Grafting experiment was performed to determine if host plant resistance is determinant of dominant population of betasatellite in cotton (several distinct strains of CLCuMuB are associated with the disease). Infected scion of Gossypium hirsutum collected from field (the source) was grafted on G. arboreum, a diploid cotton species, resistant to the disease. A healthy scion of G. hirsutum (sink) was grafted at the top of G. arboreum to determine the movement of virus/betasatellite to upper susceptible scion of G. hirsutum. Symptoms of disease appeared in the upper scion and presence of virus/betasatellite in the upper scion was confirmed via molecular techniques, showing that virus/betasatellite was able to move to upper scion through resistant G. arboreum. However, no symptoms appeared on G. arboreum. Betasatelites were cloned and sequenced from lower scion, upper scion and G. arboreum which show that the lower scion contained both CLCuMuB and ChLCB, however only ChLCB was found in G. arboreum. The upper scion contained CLCuMuB with a deletion of 78 nucleotides (nt) in the non-coding region between Arich sequence and ${\beta}C1$ gene and insertion of 27 nt in the middle of ${\beta}C1$ ORF. This study may help in investigating molecular basis of resistance in G. arboreum.

Cytological studies on Asiatic Cotton Varieties Cultivated in Korea -II. Korean Asiatic Cultivars ${\times}$ Gossypium herbaceum testers (한국동아면의 종분류에 관한 세포학적 연구 -II. 한국동아면 ${\times}$ G. herbaceum 검정종)

  • Mun-Hue Heu;Young-Am Chae;Soon-Jai Park
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.7 no.1
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    • pp.145-151
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    • 1969
  • To make clear the species of Korean Asiatic cotton, 21 Asiatic cotton varieties collected from China, Manchuria, Japan and Korea and preserved at suwon Crop Experiment Station were crossed to the tester stocks and cytological studies were made for their $F_1$ pollen mother cells. The results were summarized as follows: 1. In the all $F_1$ hybrids between the 21 collections and G. herbaceum tester stocks always one ring-four association was observed. 2. In the $F_1$ hybrids between additional 5 collections and G. arboreum tester stock ring-four or chain-four was not observed. 3. In the $F_1$ hybrids between G. herbaceum tester stocks and between G. arboreum tester stocks no ring-four or chain-four was observed, while in the $F_1$ hybrids between G. herbaceum tester stock and G. arboreum tester stock always one ring-four was observed. In the $F_1$ hybrids between collections also no ring-four or chain-four was observed. 4. From above results and together with the results reported in previous paper the species of Asiatic collections was inferred to the D.U. Gerstel's G. arboreum and their race was inferred to the J. Hutcinson's G. arboreum L. race sinense.

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Gossypii Semen oil alleviates memory dysfunction in scopolamine-treated mice (면화자 정유의 기억력 손상 완화 효과)

  • Lee, Jihye;Jung, Eun Mi;Lee, Eunhong;Jang, Gwi Yeong;Seo, Kyung Hye;Kim, Mi Ryeo;Jung, Ji Wook
    • The Korea Journal of Herbology
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    • v.36 no.2
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    • pp.1-9
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    • 2021
  • Objectives : Gossypium arboreum (cotton) is traditionally used to treat various health disorders. However, anti-amnesic effect of G. arboreum has not been reported. The objective of this study was to investigate in-vivo the anti-amnesic effects along with in vitro antioxidant and acetylcholinesterase (AChE) inhibition potential in G. arboreum seed essential oil. Methods : The essential oil of G. arboreum obtained by solid phase microextraction (SPME) techniques were identified by gas chromatography-mass spectroscopy (GC-MS). 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2'-azino-bis-(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) assay were performed to determine the antioxidant activity at various concentrations (312.5, 625, 1250, 2500, 5000, 10000 ㎍/㎖. Y-maze, passive avoidance and Morris water maze tests were carried out to evaluate improved effect on scopolamine (1 mg/kg)-induced memory dysfunction at the dose level of 50, 100 and 200 mg/kg. Donepezil (5 mg/kg) was used as a positive drug control. We performed acetylcholinesterase (AChE) activity assay in ex vivo. Results : Five volatile compounds were identified in G. arboreum. The assays of DPPH and ABTS revealed that G. arboreum increased antioxidant activity in a dose-dependent manner. G. arboreum ameliorated the percent of spontaneous alternation in the Y-maze test, shortened step-through latency in the passive avoidance test, and increased swimming time in the target zone in the Morris water maze test. In addition, G. arboreum inhibited the AChE activity. Conclusions : Based on these findings, G. arboreum may aid in the prevention and treatment of learning and memory-deficit disorders through antioxidant and AChE inhibitory activities.

A Collecting and Record of Wide Area Cultural Resources : the Case of Asian Cotton Cultural Resources (광역 문화자원의 수집과 기록 : 아시아 목화문화자원을 중심으로)

  • Noh, Shi-Hun
    • The Korean Journal of Archival Studies
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    • no.28
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    • pp.123-153
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    • 2011
  • In Asia, when cotton and cotton fabrics cultivated and produced in India of Southern Asia had spread to the whole Asia area by land and by sea, the Cotton Road and cotton fabric cultural area could be formed. In Korea, the traditional cotton (Gossypium arboreum) brought by Moon Ik-Jeom in 1363 was cultivated and then the Upland cotton (Gossypium hirsutum) brought via Japan could be produced from 1904. Especially, Gwangju/Jeonnam was the most active place in producing traditional cotton, and eventually became the center of cotton cultivation and fabric production after bringing in Upland cotton. In order to collect and record the cotton cultural resources in the broad area, the Cultural Resources Set, classified its component parts should be made first and then the collecting objects should be investigated. The collecting areas are selected based on the spreading paths and the regional significance of cotton. Since its difficulty of collecting the relevant resources from all of the places in Asia, it should be planned to share the resources through exchanges and cooperation among private, institution and organization. The relevant experts from the various fields should participate in the interdisciplinary researches which are necessary for collecting and recording of wide area cultural resources. Considering the collecting limitation of genuine relics, the digital archives should be established and then offered through a web site that everyone can use them freely by remote. It also needs to plan to display on and off-line for users to perceive the similarity, difference and interconnections of the resources with ease.