• Proceedings of the Korean Institute of Building Construction Conference
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• 2023.05a
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• pp.265-266
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• 2023

In this study, a mortar was prepared using Rhodobacter capsulatus which is forming glycocalyx and immersed in a simulated sewage environment. As a result of the experiment, it was observed that bacteria continued to grow in the mortar immersed in the simulated sewage environment, and it was confirmed that glycocalyx was formed by bacteria on the surface of mortar specimen.

• Applied Microscopy
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• v.25 no.4
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• pp.104-114
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• 1995

The epidermis of scales in the abdominal parts from freshwater- and seawater-adapted guppies(Poecilia reticulatus) were studied respectively by scanning- and transmission- electronmicroscope. In the seawater adapted group, the surface area of pavement cells is increased nearly twice as much as that of freshwater-adapted group, and the pavement cells are relatively flattened. Adaptatoin to seawater causes the increment of the density of glycocalyx in the surface of the microridge of pavement cells, as well as the well developed intercellular junctional complex(desmosome) between neighbouring filament-containing cells. Also, intercellular space between adjacent filament-containing cells is more frequently observed in the epidermis of seawater adapted guppy.

• Proceedings of the Korean Institute of Building Construction Conference
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• 2022.11a
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• pp.237-238
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• 2022

This study used Bacillus licheniformis generating glycocalyx as a provective membrane to enhance the salt-damage resistance of cement mortars. The mortar specimens with Bacillus licheniformis exhibited a comparable compressive strength development and 1.1~1.3 times higher dynamic modulus of elasticity under 300 freezing and thawing cycyles when compared with the counterpart control mortar.

• Applied Microscopy
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• v.34 no.2
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• pp.131-137
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• 2004

This study is observed the skin of the parrot fish, Oplegnathus fasciatus that related study of epidermal alternation with environmental and physiological change. It composed of supporting cells, unicellular glands and accessory cells. The supporting cells are classified into superficial cell, intermediated cell and basal cell. Superficial cell of epidermal layer is squamous or cuboidal and contain nucleus of ovoid type. And its free surface has many microridge which covered with glycocalyx. Intermediated cell is ovoid and has a nucleus of round shape. Basal cell is columnar, and nucleus is situated in the upper cytoplasm. Gland cells are classified into mucous cell and club cell. By the histochemical studies of the epidermal secretions the mucous materials react on blue in ABPAS (pH 2.5). Club cell is observed numerous vacuoles and microfilaments in the cytoplasm. The cytoplasm of chloride cells are occupied with numerous mitochondria. Pigment cells are classified into two type. The one contain pigment granules of electron dense, and the other contain reflecting platelets.

• BMB Reports
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• v.56 no.5
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• pp.314-319
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• 2023

Sepsis is a life-threatening multi-organ dysfunction with high mortality caused by the body's improper response to microbial infection. No new effective therapy has emerged that can adequately treat patients with sepsis. We previously demonstrated that interferon-β (IFN-β) protects against sepsis via sirtuin 1-(SIRT1)-mediated immunosuppression. Another study also reported its significant protective effect against acute respiratory distress syndrome, a complication of severe sepsis, in human patients. However, the IFN-β effect cannot solely be explained by SIRT1-mediated immunosuppression, since sepsis induces immunosuppression in patients. Here, we show that IFN-β, in combination with nicotinamide riboside (NR), alleviates sepsis by blocking endothelial damage via SIRT1 activation. IFN-β plus NR protected against cecal ligation puncture-(CLP)-induced sepsis in wild-type mice, but not in endothelial cell-specific Sirt1 knockout (EC-Sirt1 KO) mice. IFN-β upregulated SIRT1 protein expression in endothelial cells in a protein synthesis-independent manner. IFN-β plus NR reduced the CLP-induced increase in in vivo endothelial permeability in wild-type, but not EC-Sirt1 KO mice. IFN-β plus NR suppressed lipopolysaccharide-induced up-regulation of heparinase 1, but the effect was abolished by Sirt1 knockdown in endothelial cells. Our results suggest that IFN-β plus NR protects against endothelial damage during sepsis via activation of the SIRT1/heparinase 1 pathway.

• Applied Microscopy
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• v.21 no.2
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• pp.14-28
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• 1991

The present study was designed in order to clarify ultrastructural characteristies of the spermatogenetic process and to examine survival state of the spermatozoa introduced into the female reproductive tract after autumnal coitus in the Korean greater horseshoe bats(Rhinolophus ferrumeguinum korai). The general morphological characteristics of spermatogenesis were principally similar to those of the other mammalian species; acrosomal formation, flagellar formation, middle piece formation and concentration of the spermatozoan nucleus. The spermatozoa introduced into the vagina were found to be dead forming a vaginal plug, the opaque central core of which consisted of trapped dead spermatozoa. Some spermatozoa introduced into the uterus were observed to be phagocytized by the polymorphonuclear leucocytes infiltrated into the uterine glandular lumen. The oviductal epithelium, consisted of ciliated and secretory cells; the luminal surface of secretory cells were covered by a number of microvilli with well developed glycocalyx, suggesting a close relationship to nutrient (e.g. glycogen) supply for the spermatozoa during hibernation.

• Journal of fish pathology
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• v.18 no.2
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• pp.125-131
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• 2005

Amoebic gill disease of flounder, Paralichthys olivaceus was diagnosed at commerical culture facility in South Korea. The amoeba was identified as a species of the genus Neoparamoeba based on the morpholgical characteristics of trophozoites. Transmisson electron microscopy revealed the presence of a symbiotic organism, parasome in the cytoplasm and dense glycocalyx on the surface of the trophozoites. They lacked the boat-shaped microscales on the surface and contained numerous vacuoles and channels, mitochondria in the cytoplasm. Colonization of amoebae on gill tissue elicited extensive fusion and hyperplasia of gill lamella.

• Computers and Concrete
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• v.30 no.6
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• pp.409-419
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• 2022

The purpose of this study is to examine the effectiveness of biological repairing mortars on restoring the structural performance of a sewage culvert deteriorated by sulfate attack. The biological mortars were developed for protecting concrete structures exposed to sulfate attack based on the block membrane action of the bacterial glycocalyx. The diffusion coefficient of sulfate ions in the biological mortars was determined from the natural diffusion cell tests. The effect of sulfate-attack-induced concrete deterioration on the structural performance of culverts was examined by using the moment-curvature relationship predicted based on the nonlinear section lamina approach considering the sulfuric-acid-induced degradation of the structure. Typical analytical assessments showed that biological mortars were quite effective in increasing the sulfate-resistant service life of sewage culverts.

• Applied Microscopy
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• v.30 no.3
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• pp.295-301
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• 2000

Five kinds of sensory cells, called A1-, A2-, B-, C-, and D-type cell, respectively, are observed in the epithelial tissue of suker's infundibulum of Cephalopoda, Octopus minor. The A1-type cells lie side by with the B-type cell in the epithelium of sucker's infundibulum. In the A1-type, the nucleus shapes irregularly and the karyolymph appears dark due to its high electron density. The cytoplasm is filled with many vacuoles of various sizes ($0.04\sim0.4{\mu}m$ in diameter), which move to the apical portion of the cell to be secreted via glycocalyx. The A2-type cells are mainly found at the basal portion of the epithelium. The shape of its nucleus is similar to that in the A1-type cell, and the cytoplasm, filiform or in reticular form, shows high electron density. The B-type cell contains an ovoid nucleus and the cytoplasm where lots of vacuoles which resemble the endoplasmic reticulum and electron-dense round granules of various sizes $(0.25\sim0.6{\mu}m)$ are found. The vacuoles and granules are secreted into the free surface via glycocalyx. The C- and D-type cells in simple or stratified layer are observed at the folded portion of the sucker's epithelium. The C-type cell contains a low electron-dense elliptical nucleus, while the D-type cell has an irregular nucleus where beterochromatin is well developed.

• Journal of radiological science and technology
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• v.33 no.3
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• pp.185-192
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• 2010

Platelets originating from Megakaryocyte are sensitive to radiation along with white blood cells, and thus these platelets are used as an index of radiation hazard as they decrease in advance. Thus, when there is a scarcity of platelets, dot hemorrhage occurs and it leads to decrease of blood corpuscle and a decline in immunity. In particular, when 4~6 Gy whole body irradiation is received, after three weeks, the platelets will decrease to the lowest level, which can be a cause of death by bleeding and anemia. Therefore, this study tried to identify the mechanism of platelet damage and protection effect. The protection substance used in the experiment is Alliin, which is a component of garlic, and it was observed by an Transmission Electron Microscope(TEM) after its injection to the rat's tail vein. In the study, it was found that the cell membrane was severely damaged in a 10-day progressed platelet organ after receiving 5 Gy irradiation. It billowed as balloon-like figure and the glycocalyx became hyperplasia. The minute organ was damaged to the point that it was beyond recognition in a 20-day progressed platelet organ after receiving irradiation, and the cytoplasmic contents were exposed to epilepsy parts and outrageously damaged. Furthermore, the form of granules could also not be observed. A hole was formed in the middle, and the damaged organ was found in a 30-day progressed platelet. However, the form of granules was consistently maintained in the experiment group injecting Alliin, as with the control group, and there was no damage to the cell membrane recognized. Thus, it was possible to verify the effectiveness of radiation protection of the platelet when Alliin was injected to the blood vessel.