Electron Microscopic Study on the Integumentary Epidermis of the Parrot fish, Oplegnathus fasciatus

돌돔 (Oplegnathus fasciatus) 피부상피층에 관한 전자현미경적 연구

  • Kim, Jae-Won (Department of Marine Biology, Pukyong National University) ;
  • Baeck, Gun-Wook (Korea Inter-university Institutes of Ocean Science, Pukyong National University) ;
  • Baek, Hea-Ja (Department of Marine Biology, Pukyong National University)
  • 김재원 (부경대학교 자원생물학과) ;
  • 백근욱 (부경대학교 해양과학공동연구소) ;
  • 백혜자 (부경대학교 자원생물학과)
  • Published : 2004.06.01

Abstract

This study is observed the skin of the parrot fish, Oplegnathus fasciatus that related study of epidermal alternation with environmental and physiological change. It composed of supporting cells, unicellular glands and accessory cells. The supporting cells are classified into superficial cell, intermediated cell and basal cell. Superficial cell of epidermal layer is squamous or cuboidal and contain nucleus of ovoid type. And its free surface has many microridge which covered with glycocalyx. Intermediated cell is ovoid and has a nucleus of round shape. Basal cell is columnar, and nucleus is situated in the upper cytoplasm. Gland cells are classified into mucous cell and club cell. By the histochemical studies of the epidermal secretions the mucous materials react on blue in ABPAS (pH 2.5). Club cell is observed numerous vacuoles and microfilaments in the cytoplasm. The cytoplasm of chloride cells are occupied with numerous mitochondria. Pigment cells are classified into two type. The one contain pigment granules of electron dense, and the other contain reflecting platelets.

돌돔의 피부는 크게 상피층과 진피층으로 구분되며, 상피층은 지지세포, 선세포 및 부속세포로 구성된다. 지지세포는 표면세포, 중간세포 및 기저세포, 선세포는 점액세포와 곤봉상세포, 그리고 부속세포는 염세포가 관찰된다. 표면세포는 편평형 또는 입방형으로서 타원형의 핵을 가지고, 미세융기(microridge)들이 발달되어 있으며 glycocalyx가 관찰된다. 중간세포는 타원형에 가깝고 원형에 가까운 핵을 가진다. 기저세포는 원주형으로서 핵은 세포질의 상부에 위치한다. 점액세포는 타원형으로 세포질은 타원형의 분비과립이 대부분을 차지하며, AB-PAS (pH 2.5)에 청색으로 반응하였다. 곤봉상세포는 세포질에 많은 액포와 미세섬유다발을 관찰할 수 있다. 부속세포인 염세포 세포질의 대부분이 미토콘드리아로 가득 채워져 있다. 색소세포는 세포질에 전자밀도가 높은 색소과립들을 함유한 종류와 반사소판을 함유한 종류로 구분된다.

Keywords

References

  1. Abraham M, Iger Y, Zhang L: Fine structure of the skin cells of a stenohaline freshwater fish Cyprinus carpio exposed to diluted seawater. Tissue and Cell 33(1) : 46 54, 2001 https://doi.org/10.1054/tice.2000.0149
  2. Hawkes JW: The structure of fish skin. l. General organization. Cell Tiss Res 149 : 147 158, 1974 https://doi.org/10.1007/BF00222270
  3. Jeong YK, Moon MJ: Integumental secretory cells in goldfish, Carassius auratus. L. Korean J Electron Microscopy 24 : 1 10, 1994
  4. Karnaky KJ: Structure and function of the chloride cell of Fundulus hetroclitus and other teleosts. Am Zool 26 : 209 224, 1986 https://doi.org/10.1093/icb/26.1.209
  5. Keys A, Willmer EN: Chloride secreting cells in the gill of fishes with special refernce to the common eel. J Physiol Lond 76 : 368 377, 1932 https://doi.org/10.1113/jphysiol.1932.sp002932
  6. Kim JW, Chin P, Jin YG, Lee JS: Ultrastructure on the integumentary epidermis of the bastard halibut, Paralichthys olivaceus (Teleostei: Pleuronectidae). Korean J Electron Microscopy 32 : 121 129, 2002
  7. Korte GE: Unusual association of 'chloride cells' with another cell type in the skin of the glass catfish, Kryptopterus bicirrhis. Tissue and Cell 11 : 63 68, 1979 https://doi.org/10.1016/0040-8166(79)90006-5
  8. Lanzing WJR, Wright RG: The ultrastructure of the skin of Tilapia mossambica (Peters). Cell Tissue Res 154 : 251 264, 1974
  9. Lee JS, An CM, Huh SH: Integumentary ultrasturcture of the blenny, Pholis nebulosa (Teleostei: Pholidae). J Korean Fish Soc 33 : 148 152, 2000
  10. Lee JS, Kang JC, Baek HJ: Ultrastructure on the integumentary epidermis of the marbled sole, Limanda yokohamae (Teleostei: Pleuronectidae). Korean J Electron Microscopy 30 : 303 310, 2000
  11. Lee JS, Kim JW: Fine structure of the integumentary supporting cell and gland cell of the sea bass, Lateolablax japonicus (Teleostei: Moronidae). Korean J Electron Microscopy 29 : 417 426, 1999
  12. Leonard JB, Summers RG: The ultrastructure of the integument of the American Eel, Anguilla rostrata. Cell Tissue Res 171 : 1 30, 1976
  13. Park IS, Kim JJ, Jo UB, Park SO: Fine structural changes in the eel epidermis according to sea water adaptation. I. Epithelial cell. Korean J Zool 38 : 26 37, 1995a
  14. Park IS, Kim JJ, Jo UB, Park SO: Fine structural changes in the eel epidermis according to sea water adaptation. II. Mucous cell and club cell. Korean J Zool 38 : 38 47, 1995b
  15. Park JY, Kim IS: Structure and histochemistry of skin of mud loach, Misgurnus anguillicaudatus (Pisces, Cobitidae), from Korea. Korean J Ichthyol 11 : 109 116, 1999
  16. Payan P, Girard JP, Mayer Gostan N: Branchial ion movements in teleosts: the roles of respiratory and chloride cells. In: Hoar WS, Randall DJ, ed, Fish Physiology, XB, pp. 36 63, Academic Press, New York, 1984