• Journal of Crop Science and Biotechnology
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• v.11 no.3
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• pp.157-162
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• 2008

A total of 2,765 accessions were used as the initial set having both seed coat color and 100-seed weight data. As a result of molecular profiling using six SSR markers followed by stratification based on their usages, 335 accessions(12.1%) were selected by clustering based on UPGMA. Since 75 out of 335 accessions were mixed in phenotypic traits as a result of characterization, 260 accessions were finally set as a core set. This core set revealed nearly the same diversity compared with the other results on morphological traits of Korean soybean landraces. In total, 115 alleles(19.2 alleles per locus) were detected in the initial set and 79 alleles(13.2 alleles per locus) were detected in the core set. All 30 major alleles were present in the initial set and in the core set as well. In allele coverage, the core set was 71.4% of the initial set. These comparisons of number of alleles, gene diversity and coverage indicated that the core set represented the entire set well.

• Proceedings of the Korean Society of Crop Science Conference
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• 2022.10a
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• pp.223-223
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• 2022

Saengcheonggukjang, known as Natto in Japan, is a Korean fermented soybean food that has various bioactive compounds for antioxidant and antidiabetic activity. The development of soybean (Glycine max L.) cultivars for saengcheonggukjang production relies on the selection of seed traits that influence the quality and sensory properties. One of the important traits for cultivars is seed characteristics such as seed hardness and size. In order to select the lines for breeding good quality saengcheonggukjang varieties, several simple sequence repeat (SSR) markers and quantitative trait loci (QTLs) related to seed quality of Korean cultivars, Pungsannamulkong, Socheongja, Pungwon, Heawon, and Hoseo, were analyzed. Based on the many studies to detect stable QTLs for seed traits, we tested several QTLs related to seed size and water absorbability using SSR markers on Korean cultivars. The results showed that two regions for water absorbability of Pungsannamulkong and one region for seed size traits of Haewon and Hoseo were identified in this study. These results could have applications to soybean breeding for seed size and hardness and it is necessary to narrow it down through further study.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.42 no.4
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• pp.416-423
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• 1997

To obtain the basic information about the translocation of seed reserves of soybean[Glycine max(L.) Merrill] cultivars, 'Hill', 'Paldalkong' and 'Jangyeobkong' , the dry matter changes of emerging organs during the germination and seedling development were observed at the research farm of College of Natural Resources, Korea University, on May 26, June 5 and June 14. Mean fresh weight, dry weight and moisture content of three soybean cultivars were increased until the V2 stage in all seedling parts except cotyledons. Cotyledon dry weight was continuously decreased and the decreased amount at the V2 stage was about 80% of the cotyledon dry weight at the germination stage. Structural component of cotyledons was 22.2% of the cotyledon dry weight at the germination stage. Metabolic components of cotyledons were markedly decreased until V2 stage, and about 91% of cotyledon metabolic components at germination stage was utilized. However, those of whole seedling were increased after the V1 stage. Therefore, it appeared that role of cotyledons as nutrient supplier for germination and seedling growth was important until the V1 stage especially.

• Korean Journal of Food Science and Technology
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• v.39 no.5
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• pp.580-583
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• 2007

Little is known about the genetic variability in lutein content for soybeans (Glycine max L.) cultivated in Korea. Therefore, the objective of this study was to screen high quality soybean cultivars for lutein content. The lutein contents of 23 recommended varieties, 56 sauce varieties, 44 sprout varieties, and 40 local varieties were determined by saponification followed by reversed phase high performance liquid chromatography (HPLC). We found that IT No. 153398 and 22789, originating from sauce and local varieties, respectively, contained significantly higher amounts of lutein as compared to the other 161 samples. Moreover, relevant quantitative differences were found among the samples, which had lutein contents ranging from 83 to $402{\mu}g/100g$ in the recommended varieties, from 39 to $778{\mu}g/100g$ in the sauce varieties, from 52 to $589{\mu}g/100g$ in the sprout varieties, and from 106 to $861{\mu}g/100 g$ in the local varieties, respectively. In summary, this study provides reliable lutein data for soybean cultivars that can be used for breeding studies and the development of functional foods.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.36 no.6
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• pp.560-567
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• 1991

Photosynthetic variation in field grown soybean [Glycine max (L.) Merr. cv Hodgson78］ was studied in relation to leaf anatomical variation. Variations in mesophyll morphology were accentuated by manipulating source and sink size. At R3 stage, two treatments were started: one was thinning and continu-ous debranching(6. 5 plants rather than 26 plants per m of row and remaining plants were debranched weekly), and the other was continuous partial depodding (allowing only one pod to develop at each mainstem node). Gas exchange characteristics, mesophyll cell volume and surface area per unit leaf surface, and microclimatic parameters were measured on the intact terminal leaflet at the 10th node. Observations were made 5 times with 3 to 4 day intervals starting R4 stage. Two models were used to compute leaf photosynthetic rates: one considered no effect of mesophyll morphology on photosynthesis, and the other considered potential effects of variations in mesophyll cell volume and surface area on diffusion and biochemical processes. Seventy nine percent of total photosynthetic variations observed in the experiment was explained by the latter, while 69% of the same variations was explained by the former model. By incorporating the mesophyll morphology concept, the predictability was improved by 14.6% in the field condition. Additional Index Words: photosynthesis model, leaf anatomy, Glycine max (L.) Merr., mesophyll surface area, mesophyll cell volume.

• The Korean Journal of Food And Nutrition
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• v.33 no.6
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• pp.655-665
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• 2020

In this study, chemical information on a total of 20 individual compounds was constructed to identify isoflavones from the previous reports related with used parts(seeds, leaves, stems, pods) and products of soybean(Glycine max L.). Through constructed library and UPLC-DAD-QToF/MS analysis, a total of 19 individual isoflavones including aglycones, glucosides, acetylglucosides and malonylglucosides as major compounds was identified and quantified from 14 selected soybean seeds. Among them, genistein 7-O-(2"-O-apiosyl)glucoside and genistein 7-O-(6"-O-apiosyl)glucoside(ambocin) were identified tentatively as novel compounds in soybean seeds. Besides, among malonylglucosides, glycitein 4'-O-(6"-O-malonyl)glucoside was estimated for the first time. Total isoflavone contents were distributed from 240.21 to 445.21(mg/100 g, dry matter) and 7-O-6"-O-malonylglucosides were composed of 77.8% on total isoflavone as well as genistein derivatives were confirmed as major class. It was considered importantly that the development of isoflavone-rich varieties was necessary to strengthen their effects such as anti-inflammation, anti-cancer and menopause mitigation. The qualitative and quantitative data presented precisely in this study could be help to select and breed isoflavone-rich varieties. Furthermore, their basic isoflavone profile is expected to be applied to estimate the change of isoflavone conjugates on bioavailability after soy food supplements.

• Korean Journal of Plant Resources
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• v.35 no.5
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• pp.586-593
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• 2022

Soybean (Glycine max (L.) Merr.) is a high-protein oilseed crop, cultivated worldwide. Soybean seeds are abundant in various secondary metabolites with physiologically active. Mature seeds of 25 soybean cultivars with various morphological and physiological characteristics analyzed for secondary metabolites, such as carotenoid, isoflavone, and soyasaponin. These secondary metabolites showed various content by genotype in 25 cultivars. Total carotenoid content ranged from 1.23 to 33.78 mg/g and three cultivars, such as IT177645, PI90763 and IT234975 with black seed coat showed high levels of total carotenoid. Total isoflavones content ranged from 20.28 to 276.35 mg/100g and were detected high levels in Savoy, PI90763 and KLG16001. In addition, total soyasaponins content ranged from 33.12 to 246 mg/100g and were detected high levels in PI90763, PI86490 and IT234975. The PI90763 was showed abundant content in all of the carotenoid, isoflavones and soyasaponins. These results could be valuable information for the development of new soybean cultivars and regulation of secondary metabolites biosynthesis in soybean.

• The Korean Journal of Food And Nutrition
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• v.34 no.4
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• pp.331-339
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• 2021

With the aim of developing region specialized crops, this study was conducted to clarify effects of variant and cultivation region on antioxidative activities in various black soybean (Glycine max L.) seed tissues. Three black soybean varieties (SCEL-1, Wonheug, and Cheongja 3) were each cultivated in 4 different regions (Jeonju, Pyeongchang, Paju, and Cheonan). Harvested seeds were used to assess DPPH and ABTS radical scavenging activity, and total polyphenol, flavonoid and anthocyanin content. SCEL-1 soybean hull contained higher DPPH and ABTS radical scavenging activity (61% and 85% respectively) compared to Wonheug (40% and 50% respectively). SCEL-1 cultivated in Pyeongchang displayed the highest total polyphenol and flavonoid content (1,189 mg GAE/100g sample and 951 mg CTE/ 100g sample, respectively). Total anthocyanin content was ranked in the following order: SCEL-1>Wonheug>Cheongja 3. All black soybeans showed much higher antioxidant activity in the soybean hull than in the dehulled soybean. The antioxidant activity of black soybeans cultivated at high latitudes was high. These results suggest that the best black soybean variant for high beneficial biological activities is the SCEL-1 variant. For a complete understanding of the potential of black soybean as functional foods, we plan to further analyze their antioxidant activities in future studies.

• Korean Journal of Plant Resources
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• v.37 no.1
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• pp.71-78
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• 2024

This study was in order to select soybean (Glycine max (L.) Merrill) cultivars that are suitable for cultivating in paddy fields of the southern Korea and beneficial for mechanized harvesting with combine harvesters. Five soybean cultivars were cultivated in paddy fields to survey growth characteristics and seed yield, and evaluate the nutritional value of seed. Plant height was longer than 55 cm for 'Cheongja-3ho', 'Daewon' and 'Seonpung'. First pod height was higher than 12 cm for 'Daewon', 'Seonpung', and 'Seonyu-2ho'. Seed yields were 280 kg/10a or more for 'Daechan', 'Seonpung' and 'Daewon'. The protein content of 'Daechan' were 40.7%, which was significantly higher than those of the other cultivars, and isoflavone content was higher than 200 ㎍·g^-1 for 'Seonpung', 'Cheongja-3ho', 'Daechan', and 'Daewon'. These results suggest that 'Daechan', 'Seonpung', 'Daewon' could be suitable for cultivating in paddy fields, and 'Daewon' and 'Seonpung' could be more beneficial for mechanized harvesting with combine harvesters. Furthermore, 'Daechan' and 'Seonpung' have not only high seed yields, but also high protein and isoflavone contents, suggesting that they could be used for production of various food products.

• Research in Plant Disease
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• v.12 no.3
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• pp.213-220
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• 2006

In year 2003, a soybean(Glycine max) sample showing severe dwarfing symptom was collected from a farmers' field in Cheongsong in Korea. The results from the diagnosis of the sample by RT-PCR revealed that it was infected by Soybean dwarf virus(SbDV), SbDV-L81. This study could be the first report of the occurrence of the virus in Korea. To further characterize the virus, the partial nucleotide sequence of the genomic RNA of SbDV-L81 was determined by RT-PCR using species-specific primers. The sequences were analyzed and subsequently compared to previously characterized strains of SbDV based on the pattern of symptom expression and vector specificities. The intergenic region between ORF 2 and 3 and the coding regions of ORF 2, 3 and 4 were relatively similar to those of dwarfing strains(SbDV-DS and DP) rather than those of yellowing strains(SbDV-YS and YP). Likewise, the result from the analysis of 5'-half of the coding region of ORF5 indicated that SbDV-L81 was closely related to strains(SbDV-YP and DP) transmitted by Acyrthosiphon pisum. These data from the natural symptom and the comparisons of five regions of nucleotide sequences of SbDV suggested that SbDV-L81 might be closely related SbDV-DP.