Lutein Contents of Soybeans (Glycine max L.) Cultivated in Korea

국내 콩(Glycine max L.)자원의 lutein 함량

  • Choi, Young-Min (Department of Food Science and Technology, Chungbuk National University) ;
  • Lim, Ho (Department of Food Science and Technology, Chungbuk National University) ;
  • Woo, Sun-Hee (Department of Crop Science, Chungbuk National University) ;
  • Kim, Hong-Sik (Department of Crop Science, Chungbuk National University) ;
  • Jong, Seung-Keun (Department of Crop Science, Chungbuk National University) ;
  • Lee, Jun-Soo (Department of Food Science and Technology, Chungbuk National University)
  • 최용민 (충북대학교 식품공학과) ;
  • 임호 (충북대학교 식품공학과) ;
  • 우선희 (충북대학교 식물자원학과) ;
  • 김홍식 (충북대학교 식물자원학과) ;
  • 정승근 (충북대학교 식물자원학과) ;
  • 이준수 (충북대학교 식품공학과)
  • Published : 2007.10.31

Abstract

Little is known about the genetic variability in lutein content for soybeans (Glycine max L.) cultivated in Korea. Therefore, the objective of this study was to screen high quality soybean cultivars for lutein content. The lutein contents of 23 recommended varieties, 56 sauce varieties, 44 sprout varieties, and 40 local varieties were determined by saponification followed by reversed phase high performance liquid chromatography (HPLC). We found that IT No. 153398 and 22789, originating from sauce and local varieties, respectively, contained significantly higher amounts of lutein as compared to the other 161 samples. Moreover, relevant quantitative differences were found among the samples, which had lutein contents ranging from 83 to $402{\mu}g/100g$ in the recommended varieties, from 39 to $778{\mu}g/100g$ in the sauce varieties, from 52 to $589{\mu}g/100g$ in the sprout varieties, and from 106 to $861{\mu}g/100 g$ in the local varieties, respectively. In summary, this study provides reliable lutein data for soybean cultivars that can be used for breeding studies and the development of functional foods.

본 연구에서는 국내 콩 자원의 lutein 함량 변이를 분석함으로써 우수 품종을 선발하고 신품종 육성연구와 기능성 식품 개발을 위한 우수 품종을 선발하고자 하였다. 본 연구에서는 장려품종 23종과 육성품종 140종(장콩품종 56종, 나물콩품종 44종, 지역재래품종 40종)을 lutein 분석에 사용하였다. 검화방법을 이용하여 lutein을 추출하였고 역상 HPLC를 이용하여 분석하였다. 연구 결과 장려품 23종의 평균 lutein 함량은 $240.27{\pm}90.74{\mu}g/100g$으로 IT No. 24634가 $402.48{\mu}g/100g$으로 가장 높은 함량을 나타내었다. 장콩은 $777.73{\mu}g/100g$으로 IT No. 153398이, 나물콩은 $588.88{\mu}g/100g$으로 IT No. 25490이, 지역재래종은 $861.07{\mu}g/100g$으로 IT No. 22564가 높은 lutein 함량을 나타내었다. 장려품종과 육성품종간의 그 평균치는 통계학적으로 유의적인 차이를 나타내지 않았으나 장려품종 보다는 육성품종에서 상대적으로 높은 lutein 함량을 나타내는 품종이 많은 것으로 나타났다. 본 연구결과는 국내 콩 유전자원의 품종개량 및 고 부가가치 기능성 식품의 개발에 대한 기초 자료가 될 것으로 생각된다.

Keywords

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