• 한국작물학회지
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• 제32권4호
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• pp.409-416
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• 1987

본시험은 남부지역에 있어서 파종기 이동이 콩의 건물생산능력 및 생리, 생태적특성에 미치는 영향을 파악하여 적정 파종 한계기를 제시하고자 ＇82년∼＇84년도 3개년에 걸쳐 시험을 실시하였던바 다음과 같은 결과를 얻었다. 1. 만파할수록 개화일수, 성숙기 개화기간 등은 크게 단축되었고 품종가 차이도 인정되었으며 엽후는 만파에 의하여 두꺼워지는 반면, 엽면적은 감소하였고, CGR은 활엽기를 전후하여 뚜렷한 차이를 보였는데 7월 9일 파종기는 타 파종기의 50% 내외의 작물생자율을 나타내었다. 2 건물중은 만파할수록 현저히 감소되었으나 100 입중을 제외한 다른 수량구성요소는 5월 10일과 5월 30일간에 비슷한 경향을 나타냈다. 3. 10a당 수량은 5월 10일 파종구 225kg, 5월 30일 239kg, 6월 19일 188kg, 7월 9일은 101kg으로 5월 30일 파종구의 수량이 가장 높았으며 품종간에는 단엽콩이 파종기 이동에 의한 수량변이폭이 가장 작았다.

• Journal of Crop Science and Biotechnology
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• 제11권2호
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• pp.83-90
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• 2008

Two hundred and sixty Korean soybean landrace accessions were analyzed for polymorphism at 92 simple sequence repeat(SSR) loci. The 995 identified alleles served as raw data for estimating genetic diversity and population structure. The number of alleles at a locus ranged from three to 27 with a mean of 10.4 alleles per locus. $F_{ST}$ values estimated by analysis of molecular variance(AMOVA) using SSR data set were 0.018, 0.027, and 0.016 for usage, collection site and maturity groups, respectively, indicating little genetic differentiation. The model-based clustering analysis placed the accessions into three clusters(K=3) with 0.0503 of $F_{ST}$, indicating moderate genetic differentiation. Duncan's Multiple Range Test at K = 3 on the basis of 18 quantitative traits revealed that one cluster was mainly differentiated from the other two clusters by seed related traits and the other two clusters were differentiated from each other by biochemical traits. Genetic structure of Korean soybean landraces was differentiated by model-based clustering and supported by their phenotypic traits in part. This preliminary study could be the first step towards more efficient germplasm management and utilization of soybean landraces and helpful in association studies between genotypic and phenotypic traits in Korean soybean landraces.

• 한국작물학회:학술대회논문집
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• 한국작물학회 2017년도 9th Asian Crop Science Association conference
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• pp.146-146
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• 2017

The pod shattering or dehiscence is essential for the propagation of pod-bearing plant species in the wild, but it causes significant yield losses during harvest of domesticated crop plants. Identifying novel molecular makers, which are linked to seed-shattering genes, is needed to employ the molecular marker-assisted selection for efficiently developing shattering-resistant soybean varieties. In this study, a genetic linkage map was constructed using 115 recombinant inbred lines (RILs) developed from crosses between the pod shattering susceptible variety, Keunol, and resistant variety, Sinpaldal. A 180 K Axiom(R) SoyaSNPs data and pod shattering data from two environments in 2001 and 2015 were used to identify quantitative trait loci (QTL) for pod shattering. A major QTL was identified between two flanking single nucleotide polymorphism (SNP) markers, AX-90320801 and AX-90306327 on chromosome 16 with 1.3 cM interval, 857 kb of physical range. In sequence, genotype distribution analysis was conducted using extreme phenotype RILs. This could narrow down the QTL down to 153 kb on the physical map and was designated as qPDH1-KS with 6 annotated gene models. All exons within qPDH1-KS were sequenced and the 6 polymorphic SNPs affecting the amino acid sequence were identified. To develop universally available molecular markers, 38 Korean soybean cultivars were investigated by the association study using the 6 identified SNPs. Only two SNPswere strongly associated with the pod shattering. These two identified SNPs will help to identify the pod shattering responsible gene and to develop pod shattering-resistant soybean plants using marker-assisted selection.

• Current Research on Agriculture and Life Sciences
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• 제32권2호
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• pp.105-109
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• 2014

The soybean [Glycine max (L.) Merr.], an edible legume, has a high protein content in both its hay and grain, so it is often used as a supplement for other forages that have a deficient protein concentration. Therefore, this study investigated the forage quality and yield in the case of mixed planting of soybean and corn. The forage yield and quality were assessed for three cropping patterns: soybean mono planting, corn mono planting, and mixed planting of soybean and corn. For planting, this study used a forage corn cultivar, Kwangpyeongok, and three recombinant inbreed lines, W2, W4, and W11, selected from Glycine soja (PI483463)${\times}$G. max (Hutcheson). The mixed planting of soybean and corn produced a higher forage yield than the corn mono cropping. The crude protein and crude fat content were also increased with the mixed planting of soybean and corn when compared with the corn mono cropping. Some decrease of ADF and NDF, and increase for RFV in mixed planting of soybean and corn than corn mono cropping. Therefore, the results show that mixed planting of soybean and corn is an effective cropping system to improve the forage quality.

• 한국작물학회지
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• 제47권2호
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• pp.85-94
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• 2002

Antibodies raised against the purified p-subunit of $\beta$-conglycinin were used in immunohistochemical studies to monitor the pattern of $\beta$-conglycinin mobilization in the cotyledons during soybean [Glycine max (L.) Merr.] seed germination. Western blot analysis revealed that the break down of the $\beta$-subunit of $\beta$-conglycinin commenced as early as 2 days after seed imbibition (DAI). Concurrent with the degradation of the $\beta$-subunit of $\beta$-conglycinin, accumulation of 48, 28, and 26 kD proteolytic intermediates was observed from 2 to 6 DAI. Western blot analysis also revealed that the acidic subunit of glycinin was mobilized earlier than the basic subunit. The basic glycinin subunit was subjected to proteolysis within 2 DAI resulting in the appearance of an intermediate product approximately 2 kD smaller than the native basic glycinin subunit. In contrast to the major seed storage proteins, lipoxygenase was subjected to limited proteolysis and was detected even after 8 DAI. The first sign of $\beta$-conglycinin breakdown was observed near the vascular strands and proceeded from the vascular strands towards the epidermis. Protein A-gold localization studies using thin sections of soybean cotyledons and antibodies raised against the $\beta$-subunit of $\beta$-conglycinin revealed intense labeling over protein bodies. A pronounced decrease in the protein A-gold labeling intensity over protein bodies was observed at later stages of seed germination. The protein bodies, which were converted into a large central vacuole by 8 DAI, contained very little 7S protein as evidenced by sparse protein A-gold labeling in the vacuoles.

• Food Science and Biotechnology
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• 제15권3호
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• pp.392-398
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• 2006

Four flavonoids 1-4 and one phytosterol 5 were isolated from methanol extract of Taekwangkong, one of the soybean cultivars, and the structures of these compounds were fully characterized by physical and spectral analysis. The content of compounds 1-4 as determined by $C_{18}$ reversed phase HPLC (high-performance liquid chromatography) coupled with diode-array detector were 12.1, 624.6, 18.0, and $219.6\;{\mu}g/g$, respectively, and the total phenolic content of this cultivar was measured as 3.7 mg gallic acid equivalent per g dry material (GAB/g). Also, compound 1 showed strong radical scavenging activity in the 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) (ABTS) assay ($IC_{50}\;=\;47.6\;{\mu}M$), five-fold higher than seen in the 1,1-diphenyl-2-picrylliydrazyl (DPPH) assay. These results lead to the conclusion that soybean not only has many phytoestrogens but also has potent antioxidant activity.

• The Plant Pathology Journal
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• 제38권6호
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• pp.603-615
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• 2022

Soybean (Glycine max (L) Merr.) provides plant-derived proteins, soy vegetable oils, and various beneficial metabolites to humans and livestock. The importance of soybean is highly underlined, especially when carbon-negative sustainable agriculture is noticeable. However, many diseases by pests and pathogens threaten sustainable soybean production. Therefore, understanding molecular interaction between diverse cultivated varieties and pathogens is essential to developing disease-resistant soybean plants. Here, we established a pathosystem of the Korean domestic cultivar Kwangan against Pseudomonas syringae pv. syringae B728a. This bacterial strain caused apparent disease symptoms and grew well in trifoliate leaves of soybean plants. To examine the disease susceptibility of the cultivar, we analyzed transcriptional changes in soybean leaves on day 5 after P. syringae pv. syringae B728a infection. About 8,900 and 7,780 differentially expressed genes (DEGs) were identified in this study, and significant proportions of DEGs were engaged in various primary and secondary metabolisms. On the other hand, soybean orthologs to well-known plant immune-related genes, especially in plant hormone signal transduction, mitogen-activated protein kinase signaling, and plant-pathogen interaction, were mainly reduced in transcript levels at 5 days post inoculation. These findings present the feature of the compatible interaction between cultivar Kwangan and P. syringae pv. syringae B728a, as a hemibiotroph, at the late infection phase. Collectively, we propose that P. syringae pv. syringae B728a successfully inhibits plant immune response in susceptible plants and deregulates host metabolic processes for their colonization and proliferation, whereas host plants employ diverse metabolites to protect themselves against infection with the hemibiotrophic pathogen at the late infection phase.

• 한국초지조사료학회지
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• 제9권2호
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• pp.113-118
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• 1989

This experiment was carried out to compare chemical composition, TDN yield of corn-soybean intercropping and corn monocropping forage plants at different harvesting time and obtained the following results. 1. In both cropping systems, the content of chemical composition of forage were changed same pattern in growing stage. The content of crude protein in corn-soybean intercropping forage at yellow stage increased more than that of corn nonocropping forage, while the crude fat in corn monocropping forage plants increased than that of corn-soybean intercropping forage plants at mature stage. 2. The crude fiber, crude ash, ADF content of forage plants in both cropping system decreased same pattern in growing period, however, NFE content of forage increased with maturity. 3. TDN yield of corn-soybean intercropping and corn monocropping forage plants at yellow stage obtained similar results and TDN yield per 10a in intercropping and monocropping were 1006.lkg and 978.6kg, respectively. 4. Consequently, corn-soybean interaopping system could be increased crude protein yield without decreasing of dry matter yield in comparison with corn monocropping system for corn silage.

• 한국환경농학회지
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• 제19권5호
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• pp.375-381
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• 2000

Quantifying the changes of soil microbial biomass and activity of enzymes are important to understand the dynamics of active soil C and N pools. The dynamics of soil microbial biomass C and N and the activity of enzymes over entire growth period of soybean-(Glycine max (L) Merr.)-wheat (Triticum aestivum L.) sequence on a Typic Haplustert as influenced by organic manure and inorganic fertilizer N were investigated in a field experiment. The application of farmyard manure at 4 to 16 $Mg{\cdot}ha^{-1}\;y^{-1}r^{-1}$ along with fertilizer nitrogen at 50 or 180 $kg{\cdot}ha^{-1}$ increased the mean soil microbial biomass from 1.12 to 2.05 fold over unmanured soils under soybean-wheat system. Irrespective of organic and chemical fertilizer N application, the soil microbial biomass was maximum during the first two months at active growing stage of the crops and subsequently declined with crop maturity. The mean annual microbial activity was significantly increased when manure and chemical fertilizer at 8 $Mg{\cdot}ha^{-1}$ and 50/180 N $kg{\cdot}ha^{-1}$, respectively were applied. The C turnover rate decreased by 47 to 72 % when the level of farmyard manure was increased from 4 to 8 and 16 $Mg{\cdot}ha^{-1}$. There were significant correlations between biomass C, available N, dehydrogenase, phosphatase and yield of the crops.

• 한국작물학회지
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• 제23권1호
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• pp.86-89
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• 1978

중과 일년생잡초와의 경합시기와 잡초경합에 의한 콩의 수량감소를 추정하기 위하여 파종후 2주간격으로 10주까지 그리고 전생육기간동안 잡초를 제거하지 않고 그 이후 제초하였을 때 콩의 생육 및 수량에 미치는 영향을 조사하였으며 그 결과를 요약하면 다음과 같다. 1. 본실험포장의 우점잡초는 피, 바랭이, 참방동산이, 여뀌, 쇠비름, 쑥, 명아주 등이었다. 2. 잡초와의 경합기간이 길어질수록 콩의 생장이 억제되어 콩의 LAI와 CGR이 크게 감소되었다. 3. 초장, 주당분지수 및 주당협수도 잡초와의 경합기간이 길어짐에 따라 감소하는 경향이었다. 4. 콩의 수량은 파종후4주 이전까지 잡초를 제거하기 시작한 경우 잡초외 경합에 의하여 3∼6％ 감소되었으나 8주이후에 서초를 하면 수양감소가 심하였다. 5. 중의 수량과 초장을 제외한 LAI. CGR. 주당협수 및 분지수간에는 유의상관을 나타내었다.