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Isolation and Characterization of Phytochemical Constituents from Soybean (Glycine max L. Merr.)  

Lee, Jin-Hwan (Yeongnam Agricultural Research Institute, National Institute of Crop Science, RDA)
Baek, In-Youl (Yeongnam Agricultural Research Institute, National Institute of Crop Science, RDA)
Kang, Nam-Suk (Yeongnam Agricultural Research Institute, National Institute of Crop Science, RDA)
Ko, Jong-Min (Yeongnam Agricultural Research Institute, National Institute of Crop Science, RDA)
Han, Won-Young (Yeongnam Agricultural Research Institute, National Institute of Crop Science, RDA)
Kim, Hyun-Tae (Yeongnam Agricultural Research Institute, National Institute of Crop Science, RDA)
Oh, Ki-Won (Yeongnam Agricultural Research Institute, National Institute of Crop Science, RDA)
Suh, Duck-Yong (Yeongnam Agricultural Research Institute, National Institute of Crop Science, RDA)
Ha, Tae-Joung (Yeongnam Agricultural Research Institute, National Institute of Crop Science, RDA)
Park, Ki-Hun (Division of Applied Life Science (BK21 program), Department of Agricultural Chemistry, Research Institute of Life Science, Gyeongsang National University)
Publication Information
Food Science and Biotechnology / v.15, no.3, 2006 , pp. 392-398 More about this Journal
Abstract
Four flavonoids 1-4 and one phytosterol 5 were isolated from methanol extract of Taekwangkong, one of the soybean cultivars, and the structures of these compounds were fully characterized by physical and spectral analysis. The content of compounds 1-4 as determined by $C_{18}$ reversed phase HPLC (high-performance liquid chromatography) coupled with diode-array detector were 12.1, 624.6, 18.0, and $219.6\;{\mu}g/g$, respectively, and the total phenolic content of this cultivar was measured as 3.7 mg gallic acid equivalent per g dry material (GAB/g). Also, compound 1 showed strong radical scavenging activity in the 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) (ABTS) assay ($IC_{50}\;=\;47.6\;{\mu}M$), five-fold higher than seen in the 1,1-diphenyl-2-picrylliydrazyl (DPPH) assay. These results lead to the conclusion that soybean not only has many phytoestrogens but also has potent antioxidant activity.
Keywords
phytoestrogen; Glycine max; Taekwangkong; HPLC; total phenolic content; ABTS;
Citations & Related Records
Times Cited By KSCI : 2  (Citation Analysis)
Times Cited By Web Of Science : 6  (Related Records In Web of Science)
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