• Journal of the Korean Society of Food Science and Nutrition
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• v.37 no.1
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• pp.72-75
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• 2008

Quality properties of red ginseng prepared with different cultivation years of fresh ginseng produced in Punggi region were investigated. Fresh ginseng cultivated for 4, 5, or 6 years was steamed for 3.5 hr and dried for 24 hr at $60{\sim}65^{\circ}C$ and subsequently for $3{\sim}4$ days at $40^{\circ}C$ under commercial conditions. Compared to 6 years-old roots, the five years-old roots showed similar or some lower quality properties in terms of color, appearance, diameter, and inside quality, but higher ones in length and yield of prepared red ginseng. In particular, the levels of ginsenoside $Rg_3$ and $Rh_2$, which are known as specific components in red ginseng, were the highest in 5 years-old roots. The result shows that fresh ginseng of 5 years-old roots produced in Punggi region can be utilized as a raw material for the manufacture of high-quality red ginseng.

• Journal of Practical Agriculture & Fisheries Research
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• v.15 no.1
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• pp.183-202
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• 2013

The demand of ginseng which has attracted many people for a long time has expanded further with great spotlight ; but it has caused anxiety of some safety-sensitive customers due to unavoidable pesticides and its weakness for disease and insect pest. On the other hand, sluggish studies on effective doses of ginseng and red ginseng which is produced and processed after hardship have worsened confusion of customers. Against this backdrop, this study is about to find out measures for safe ginseng cultivation and effective dose of white or red ginseng which are safely produced and reaches meaningful conclusions as follows ; As for a study to minimize the use of chemical fertilizers and pesticides or to change them into environmentally-friendly products, ginseng cultivation utilizing Lime Sulfur complex might be an alternative. The effects of Lime Sulfur complex are great on ginseng seeding with under 200 times compound KHCO3 and five-year-old ginseng with over 200 times compound using NaHCO3. When using with green materials like Bordeaux mixture, there would be great potential to realize ginseng without pesticide use.

• Journal of the Korean Applied Science and Technology
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• v.35 no.4
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• pp.995-1002
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• 2018

Hairy root culture of ginseng is industrially prospected because the cultivation period of ginseng is relatively long. In this study, the effect of medium concentration and sucrose concentration on hairy root culture of ginseng was evaluated. The optimization of ginseng hairy roots transformed by Agrobacterium rhizogene were performed liquid medium. The MS(Murashinge & Skoog basal medium) concentration was selected with 1/2 strength MS and the optimal sucrose concentration was determined at 2-3%(w/v). At the optimum culture condition, The yield (the ratio of weight of grown hairy root cultures to weight of fresh ginseng hairy roots) and production rate of ginseng root were 19.42 times and 5.73 g/l-day. The major ginsenosides were Rb group, Re and Rg1. The produced total ginsenoside content in the solid medium was 9.87 (mg/g) and increased 1.34 times in the liquid medium (13.23 mg/g). In solid culture, the contents of ginsenosides Rb, Re and Rg1 were 2.14, 3.65 and 1.87 mg/g, respectively. In liquid culture, the contents of ginsenosides Rb, Re and Rg1 were 3.54, 4.12 and 2.63 mg/g, respectively.

• Journal of Applied Biological Chemistry
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• v.65 no.4
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• pp.253-259
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• 2022

The aim of this study was to investigate the correlation between growth characteristics and ginsenoside contents of 4-year-old wild-simulated ginseng cultivated in different regions. Most of the soil properties except for available phosphate showed significantly higher in Pyeongchang than in other cultivation sites. The growth characteristics except for root length and number of rootlets showed significantly higher in Pyeongchang than in other cultivation sites. In the case of 8 ginsenoside contents, the content of F2-AS was significantly higher in Muju than in other cultivation sites and the content of F1 in Yeongju was significantly high. In Yeongwol, the contents of Rb1 and Re-p were significantly high and the content of Ro in Pyeongchang showed significantly higher than in other cultivation sites. Root length and soil pH did not show a significant correlation with any soil properties and growth characteristics of wild-simulated ginseng, respectively. Most of the growth characteristics showed significantly positive correlations with electrical conductivity, organic matter content, total nitrogen content, exchangeable cations (K⁺, Ca²⁺, Mg²⁺), and cation exchange capacity. Rb1 and Re-p showed significantly negative correlations with most of the growth characteristics of wild-simulated ginseng except for the number of rootlets. Ro showed a significantly positive correlation with stem length, number of leaflets per stem, leaflet length, leaflet width, and root diameter. The results of this study probably will help to provide useful information on the establish a quality standard by investigate correlation analysis between growth characteristics and ginsenoside content of 4-year-old wild-simulated ginseng.

• Korean Journal of Medicinal Crop Science
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• v.20 no.4
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• pp.217-221
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• 2012

This research was conducted to investigate the influence of various organic substrates on growth and yield of ginseng seedling grown organically in the closed plastic house. The pH and EC of substrates used for organically ginseng cultivation ranged 5.93~6.78 and 0.03~0.15 dS/m respectively. The concentrations $NH_4$-N and $NO_3$-N respectively was 14.01~68.63 mg/L, 5.60~58.83 mg/L. The average quantum of the closed plastic house was range from 10 to 16% of natural light. In July and August, the maximum temperature of the closed plastic house did not exceed 30 and the average temperature was maintained within 25 lower than the field because air conditioning ran. The PPV-1 and PPV-2 bed soil substrates produced higher stem length, stem diameter, shoot fresh weight and leaf area than those of conventional culture. In PPV-2 bed soil substrates, root fresh weight and root diameter was the highest. The root fresh weight of PPV-2 bed soil substrates in closed plastic house was maximum 25% heavier than the conventional cultivation. The results of this experiment will be utilized for making new substrate application for organic ginseng culture in the plastic house.

• Journal of Biosystems Engineering
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• v.38 no.4
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• pp.270-278
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• 2013

Purpose: Using agricultural machinery was not easy in the conventional shading structure, specified as a standard facility by standard cultivation methods for ginseng. Thus, this study designed the new types of facility allowing machine access by modifying the conventional type. Methods: Two types of facility (i.e. wide roof type and long & short roof type) were designed and installed in an experimental site to evaluate its growing environments and applicability of riding-type cultivator. Results: From the results of incoming light measurement, all three types (i.e. two new types and a conventional type) of shading structures blocked the incoming light after 9:00 am. The temperature distribution inside the new types was similar with the one in the conventional type, so the growth of ginseng was in good condition in all three types of facility. The riding-type cultivator was operated well with the low speed first gear of 0.13 m/s in the new types. However, a long & short typed roof needs to be raised 18 cm height in order to use the cultivator. Conclusions: With the results of this study, the new types of roof can be used in the ginseng farm in that they satisfied the growing environments for ginseng and the needs for agricultural mechanization.

• Food Engineering Progress
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• v.21 no.3
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• pp.201-207
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• 2017

It is well-known that cultivated wild Panax ginseng has anti-inflammatory effect. However, a comparative study on cultivation period vs biofunctionality is currently lacking. In this study, 70% ethanol extracts of 3-years (yrs)-, 5-yrs-, or 7-yrs-old cultivated wild ginseng were evaluated for their inhibitory effects on RAW264.7 murine macrophages. Specifically, the production of pro-inflammatory cytokines (interleukin-6 [IL-6] and tumor necrosis factor-alpha [TNF-${\alpha}$]), the expression of surface proteins (CD80, CD86, and MHC-II), and the phagocytic properties were investigated. RAW264.7 cells were induced by 500 ng/mL of lipopolysaccharide (LPS) and treated with 0.1, 1, and 10 ppm of samples. LPS-induced IL-6, TNF-${\alpha}$ and surface proteins in all samples were down-regulated in a dose-dependent manner. Both IL-6 and TNF-${\alpha}$ were significantly reduced at 10 ppm of the 7-yrs-old sample compared to 10 ppm of 3-yrs- and 5-yrs-old samples. CD80 and CD86 were also reduced at 10 ppm of all samples, and there was no difference among samples. The phagocytosis has no difference except in 10 ppm of 3 yr-old sample. The results suggest that cultivated wild ginseng extract has anti-inflammatory effect without decreasing phagocytosis.

• Korean Journal of Medicinal Crop Science
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• v.22 no.6
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• pp.469-474
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• 2014

This study was performed to investigate the effects of sowing density and number of seeds sown on the emergence rate and growth characters of Panax ginseng C. A. Meyer under direct sowing cultivation in a blue plastic greenhouse. Ginseng seedlings, derived from seeds sown directly at different densities (90, 108, 135, and 162 seeds per $162m^2$), were cultivated in sandy loam soil within a blue plastic greenhouse. In contrast to the emergence rate, which decreased with an increase of sowing density, number of survival plant showed an increasing trend. Interestingly, the emergence and number of survival plant were significantly enhanced when 2 or 3 seeds were sown per hole compared with when one seed was sown per hole. Growth of the aerial parts of ginseng were not markedly influenced by sowing density or the number of seeds sown. However, chlorophyll content (SPAD values) increased with an increase in sowing density. Root parameters, such as root length, diameter, and weight, and the number of lateral roots decreased with an increase in sowing density, but were not noticeably influenced by the number of seeds sown. Total saponin content was the highest in the treatment plot containing 135 seeds. Similarly, the content of each ginsenoside was also tended to be higher in this treatment than in other treatment plots. On the basis of the results obtained in this study, it was possible to determine the optimal sowing density and seed number for the direct sowing cultivation of ginseng in blue plastic greenhouse.

• Journal of Ginseng Research
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• v.43 no.4
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• pp.654-665
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• 2019

Background: Panax ginseng Meyer has widely been used as a traditional herbal medicine because of its diverse health benefits. Amounts of ginseng compounds, mainly ginsenosides, vary according to seasons, varieties, geographical regions, and age of ginseng plants. However, no study has comprehensively determined perturbations of various metabolites in ginseng plants including roots and leaves as they grow. Methods: Nuclear magnetic resonance ($^1H$ NMR)-based metabolomics was applied to better understand the metabolic physiology of ginseng plants and their association with climate through global profiling of ginseng metabolites in roots and leaves during whole growing periods. Results: The results revealed that all metabolites including carbohydrates, amino acids, organic acids, and ginsenosides in ginseng roots and leaves were clearly dependent on growing seasons from March to October. In particular, ginsenosides, arginine, sterols, fatty acids, and uracil diphosphate glucose-sugars were markedly synthesized from March until May, together with accelerated sucrose catabolism, possibly associated with climatic changes such as sun exposure time and rainfall. Conclusion: This study highlights the intrinsic metabolic characteristics of ginseng plants and their associations with climate changes during their growth. It provides important information not only for better understanding of the metabolic phenotype of ginseng but also for quality improvement of ginseng through modification of cultivation.

• Journal of the Ergonomics Society of Korea
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• v.36 no.5
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• pp.545-557
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• 2017

Objective: The aim of this study was to find risk factors in cultivating ginseng based on risk assessments and suggest safety measures for main risks. Background: Safety education and training is one of the practical and effective methods to prevent occupational accidents and injuries. In agricultural sector, there are few contents of safety education as compared to other industries. Especially, farm work has different cultivation characteristics according to the crops, so it needs special education materials for each crop. Among the various types of crops, ginseng contains various risk factors due to its long cultivating period and unique environment. Therefore, safety education material specified for ginseng is necessary to improve ginseng farmers' safety. Method: Risk assessment for cultivating tasks of ginseng was carried out through data obtained from various methods (site survey, interview, literature survey). To improve objectivity, the risk assessment was applied with 3-criteria (researcher estimate, interview, previous research results). Finally, the three high-risk tasks were selected and safety measures for those tasks were provided. Results: Three tasks, such as 'Mounting, maintenance and removing supports', 'Pest control' and 'Harvest', were selected as risky tasks among total tasks. (1) In 'Mounting' and maintenance and removing supports', the farmers found to be exposed to the risks of musculoskeletal disorders and accidents related to operating the tablet machine. (2) In 'Pest control', agrichemical poisoning, musculoskeletal disorders and hyperthermia were main risks. Finally, (3) In 'Harvest', the farmers are mainly exposed to the possibility of accidents of agricultural machines and risks of musculoskeletal disorders. Thus, it needs to apply appropriate safety measures to those risky tasks, such as safety guidelines, convenience equipment, protective kit, and so on. Conclusion: This study can be used as basic data for agricultural safety and expected that it would be useful for further study. In addition, the results of the research will be produced in the form of animation, which will enhance the safety consciousness for aged farmers. Application: The result of this study can be used in developing safety education materials for ginseng farmers which is essential to prevent occupational accidents and injuries among ginseng farmers.