• 생약학회지
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• 제30권3호
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• pp.306-313
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• 1999

Polyphenol Oxidase(PPO) was purified from an extract of Ginkgo biloba leaves by ammonium sulfate fractionation followed by sephadex G-150 column chromatography, which resulted in a 18-fold increase in specific activity. The enzyme was most active at pH 8.5 and the temperature optimum for the PPO catechol oxidation reaction was $45^{\circ}C$. Heat inactivation studies showed that heating for 7, 9 and 48 min, at 80, 70 and $60^{\circ}C$ respectively caused a 50% loss in enzymatic activity and that the enzyme was completely inactivated after heat treatment at $90^{\circ}C$ for 60 min. Km values of the PPO for catechol, hydroquinone and 4-methylcatechol derived from Lineweaver-Burk plots were $6.06\;{\times}\;10^{-4}M,\;1.02\;{\times}\;10^{-3}M,\;1.41\;{\times}\;10^{-3}M$ respectively. Of the substrates tested, 4-methylcatechol was oxidized most readily and the enzyme did not oxidize monophenols. The enzyme datalyzed browning reaction was completely inhibited in the presence of reducing reagents, namely ascorbic acid, cysteine, glutathione, 2-mercaptoethanol, potassium metabisulfite at 0.5 mM level. Sodium chloride showed very little inhibition effect on Ginkgo biloba leaves PPO. Lineweaver-Burk analysis of inhibition data revealed that the inhibition by cysteine, 2-mercaptoethanol, potassium cyanide was competitive with ki values of $1.1\;{\times}\;10^{-5}M,\;2.4\;{\times}\;10^{-5}M,\;8\;{\times}\;10^{-5}M$, respectively. Among the divalent cations, $Cu^{2+}ion$ was a strong activator on PPO and $Mn^{2+}ion$ was little or no effect on PPO activity $Ni^{2+}ion$ was an inhibitor on PPO.

• 한국유기농업학회지
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• 제21권3호
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• pp.363-372
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• 2013

가지 담배가루이 친환경 방제기술을 개발하고자, 난황유 등 8종의 천연물질을 가지 정식 후 7일 간격으로 살포하면서 생육을 조사한 결과 님제처리에서 가장 양호한 경향이었다. 담배가루이 성충과 약충의 발생은 디노테퓨란약제처리에서 가장 적었고, 친환경자재 중에서는 님제 처리에서 가장 적었다. 수량은 디노테퓨란 약제처리에서 가장 많았고, 친환경 자재에서는 님제, 고추씨유 등이 많았으며, 고삼제 은행과육 등에서 적었다. 님제 처리의 경우 무처리에 비하여 69% 많았고, 디노테퓨란약제에 비해 52% 낮은 수량을 나타내었다.

• 농업과학연구
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• 제32권1호
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• pp.1-8
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• 2005

본 연구는 교통량이 가로수 식재지 토양의 화학적 특성에 미치는 영향을 파악하기 위하여 실시되었다. 이를 위해 대전시에 위치한 은행나무와 양버즘나무 가로수 주변 토양의 화학적 특성을 조사 분석하였다. 교통량이 많은 지역의 토양 pH는 7.0 내외로 높게 나타났으며, 이는 제설용으로 살포되고 있는 염화칼슘이나 콘크리트로부터 용해되어 흘러나오는 알칼리성분 및 염기성 분진 때문으로 사료된다. 토양 중 Fe, Cd, Cu, Zn, 그리고 Pb 농도는 충남대학교 지역에서 보다 교통량이 많은 대전역전과 대화공단지역에서 높게 나타났다. 이와 같은 결과는 자동차의 타이어 및 부품의 합금 등으로 이용된 물질들이 도로 주행 시 마찰 또는 마모되어 도로주변으로 배출되었기 때문으로 사료된다.

• 약학회지
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• 제49권2호
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• pp.140-146
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• 2005

Candida albicans, a polymorphic fungus, causes systemic and local infections. Recent reports show that the fungus is a main etiological agent for the arthritis. For trea tment, antifungal drugs and/or rheumatoid drugs are used, but resistance and side effects limit application of the drugs. In search of new sources for treatment of the fungal arthritis, we choose Egb 761 (extract of Ginkgo leaves 761), one of the most popular over-the-counter herbal medicines. The Egb 761 contains two major ingredients such as terpene and flavonoid. In the present study, we examined if the terpene portion of Egb 761 had anti-inflammatory activity against C.albicans-caused arthritis. The terpene was extracted with combination of methanol and water from the Egb 761, followed by gel-permeation chromatography. Presence of terpene was determined by the Salkowski colorimetric method and HPLC analysis. For an animal model of inflammation induction, mice were given an emulsion form of C.albicans cell wall mixed with Complete Freund's Adjuvant (CFA) by footpad-injection. Results showed that intraperitoneal administration of the water-soluble portion that contained terpene and flavonoid reduced the inflammation. Whereas the terpene had anti-inflammatory activity, flavonoid portion had no such activity, For determination of possible mechanism of the activity, the terpene seemed to be suppression of nitric oxide (NO) production from LPS-treated macrophages. Taken together the Ginkgo terpene may have anti-inflammatory effect against C.albicans-caused arthritis, possibly by blocking NO production.

• 한국산림과학회지
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• 제95권5호
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• pp.591-594
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• 2006

To have better urban forests in future we are focusing on two major issues like other countries. How we could have more urban forest-quantitative approach-against the expanding of urbanization, and how we could produce better urban forest-qualitative approach-to give a chance in contact with better nature for urban people. Prime surveys on urban forests have been carried out in 6 major cities since 1991, and the city of Daegu which is located on the east southern area of Korea have been case studied for qualitative approaches during last 3 years. Major species of the remnant forests were Pinus densiflora, Pinus rigida, Pinus thunbergii and Robinia pseudoacacia. Approximately 20% of the surveyed forests were lost their structure as forests caused by illegal farming, facilities and as the bare ground by excessive visitors. Generally speaking the condition of forests for biotope was very poor in overall. From two years data of monitoring of birds, we found that wooded parkways and woodland squares had more species than street tree areas. Nests were found mainly on the branch of Zelkova serrata, Pinus densiflora and Quercus myrsinaefolia. The size of urban forests mainly determined the species richness of birds. According to the oxygen output and carbon input by photosynthesis measurement, the capability was in order of Platanus occidentalis, Zelkova serrata and Ginko biloba. Air conditioning effects of trees through transpiration measurement was found and the quality of forests also related to the reducing of urban heat-island.

• 한국버섯학회지
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• 제7권4호
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• pp.163-167
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• 2009

최근 국제원자재가 상승에 따라 버섯 배지 가격이 50% 이상 상승하여 톱밥 대체 배지의 개발이 절실한 실정으로 은행 껍질을 이용하여 버섯 배지로 개발하기 위해 수행한 결과를 요약하면 다음과 같다. 은행껍질의 질소 함량은 0.32%로 포플러 톱밥 0.09%에 현저히 높았으며, 시험관을 이용한 균사 생장은 은행껍질 10~30% 첨가시 93~97mm/28일로 대조구101mm/28일에 비해 늦었다. 은행껍질 10% 첨가시 850ml PP병의 균배양일수는 18일로 대조구와 같았지만 20~30% 첨가시 2일 지연되었고, 초발이 소요일수는 은행껍질 10~30% 첨가시 7일로 대조구보다 1일정도 빨랐다. 병당 수량은 대조구 70.8g/병에 비해 은행잎박 10~30% 첨가시 80.5~91.8g/병으로 14~30% 증수되었고, 배양 및 생육일수는 은행껍질 10% 첨가시 30일이 소요되었다. 따라서 미소느타리 병재배시 은행껍질 10%를 첨가하는 것이 재배일수 단축 및 수량 증수 효과가 있는 것으로 사료된다.

• Asian-Australasian Journal of Animal Sciences
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• 제26권4호
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• pp.517-522
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• 2013

The effect on methanogens attached to the surface of rumen ciliate protozoa by the addition of plant extracts (pine needles and ginkgo leaves) was studied with particular reference to their effectiveness for decreasing methane emission. The plant extracts (pine needles and ginkgo leaves) were added to an in vitro fermentation incubated with rumen fluid. The microbial population including bacteria, ciliated-associated methanogen, four different groups of methanogens and Fibrobacter succinogenes were quantified by using the real-time PCR. Gas profiles including methane, carbon dioxide and hydrogen, and runinal fermentation characteristics were observed in vitro. The methane emission from samples with an addition of individual juices from pine needles, ginkgo leaves and 70% ethanol extract from ginko leaves was significantly lower (p<0.05, 27.1, 28.1 and 28.1 vs 34.0 ml/g DM) than that of the control, respectively. Total VFAs in samples with an addition of any of the plant extracts were significantly lower than that of the control (p<0.05) as well. The order Methanococcales and the order Methanosarcinales were not detected by using PCR in any incubated mixtures. The ciliate-associated methanogens population decreased from 25% to 49% in the plant extacts as compared to control. We speculate that the supplementation of juice from pine needles and ginkgo leaves extract (70% ethanol extract) decreased the protozoa population resulting in a reduction of methane emission in the rumen and thus inhibiting methanogenesis. The order Methanobacteriales community was affected by addition of all plant extracts and decreased to less than the control, while the order Methanomicrobiales population showed an increase to more than that of the control. The F. succinogenes, the major fibrolytic microorganism, population in all added plant extracts was increased to greater than that of the control. In conclusion, pine needles and ginkgo leaves extracts appear to have properties that decrease methanogenesis by inhibiting protozoa species and may have a potential for use as additives for ruminants.

• 한국미생물·생명공학회지
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• 제34권3호
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• pp.265-272
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• 2006

피부 여드름 치료, 예방을 위한 천연물의 활성평가를 시도하였다. 335종의 약용 및 야생식물의 다양한 부위로부터 500종의 추출물을 조제하여, 여드름 원인세균인 Propionibacterium acnes에 대한 생육저지 활성을 평가한 결과, 21종 식물체에서 조제한 25종 추출물에서 활성을 확인하였다. 500종 추출물 중, 홀아비꽃대(지상부) 추출물에서 가장 강력한 활성이 나타났으며, 방기, 고삼, 오수유, 은행(씨), 상백피, 독활 및 호장근 추출물에서도 우수한 활성을 확인하였다. 특히 고삼, 상백피, 독활 등의 항여드름 활성은 기존 보고 및 민간요법과도 잘 일치하며, 홀아비꽃대 및 방기 추출물의 항여드름 균 활성은 본 연구에서 처음으로 확인하였다. 고삼을 대상으로 한 활성물질 분리결과, kuraridin과 kurarinone의 주요 활성물질임을 확인하였다. 본 연구결과는, 안정성이 확보된 식물추출물로부터 새로운 피부여드름 치료제 개발이 가능함을 제시하고 있으며, 현재 고삼 추출물 및 활성물질의 세포 독성 및 작용기전에 대한 연구가 진행중이다.

• 한국환경복원기술학회지
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• 제21권4호
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• pp.1-10
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• 2018

Use of de-icing salts results in accumulation of high concentrations of ions on roadside soils and tree. The purpose of this study isto determine translocation of seasonal impact of exchangeable cations originating from de-icing salt on roadside surface soil-plant influenced by the intensity of foliar damage (NY = 0-25%, SY = 26-50%, CY = 51-75%) of trees. This paper investigated the concentration of four exchangeable cations ($K^+$, $Ca^{2+}$, $Na^+$, and $Mg^{2+}$) on the roadside surface soil. The tree (Ginko biloba) samples were collected from the Konkuk and Judeok intersections in Chung-ju city. The sequential extraction procedure was applied to 120 soil samples of the soilsurface and 30 tree samples. Four cation exchange ions were determined by ICP-OES. The content of four exchangeable cations present on roadside soil was found to be the lowest in NY but highest in CY from tree pits in the order of NY < SY < CY. Especially, the results were apparent during spring time compared to other seasons. Soil collected from tree pits had the highest concentration of $Ca^{2+}$ possibly due to a higher volume of traffic on those streetsresulting in splashing of more calcium chloride ($CaCl_2$). The analysis of three exchangeable cations ($K^+$, $Mg^{2+}$, and $Na^+$) in the tree leaves revealed higher levels than roadside surface soil when foliar damage ratio increased in the order of NY < SY < CY in summer. In addition, a strong positive linear relationship was observed between the concentration of exchangeable cations in soil and trees. It is hypothesized that the results of this study can be a valuable baseline for managing de-icing salt on roadside soil and trees, in order to mitigate the salt stress that can damage the roadside soil and trees.

• 한국조경학회지
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• 제32권4호
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• pp.7-20
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• 2004

This study was executed to find out how to improve the planting and use of landscaping plants in Chuncheon. The number of street trees was 17,877 in 2003. The major species of street trees were Ginkgo biloba(52.7％), Prunus sargentii(15.3％), Zelkova serrata(8.9％), Platanus occidentalis(7.3％). Salix babylonica and Platanus occidentalis were decreased, while Prunus sargentii and Zelkova serrata were increased. Salix babylonica is a good species for a lakeside city, but its seeds cause allergies to some people. Most of the trees have been removed in Chuncheon. The planting of Salix babylonica(♂) resulted in no problems, and it should be propagated to plant. The soil of several areas was analysed to investigate soil conditions. The outskirts of city had good conditions in pH, organic matter and minerals, but the downtown areas had high alkalic, due to alkali from footpath concrete blocks. Appropriate pruning adds to the aesthetic and prolongs the useful life; it also requires less managing of insects and diseases to maintain good healthy of street trees. Street trees were not properly pruned due to electric lines and shortage of pruning information. The pruning was controlled by Korea Electric Power Co, which has no pruning information. Pruning must be maintained by a professional landscape company to maintain good shape such as with Bonsai. The Shrubs planting zone between street trees and other trees, and preservation plates were established for healthy of street trees. They have to be repaired and maintained well to keep better environmental conditions. The number of tree and flower species of 68 schools in Chuncheon were 12 and 16, respectively. The species that showed high preference were Juniperus chinensis, Pinus koraiensis, Pinus densiflora, Rosa centifolia, Forsythia koreana, and Rhododendron schlippenbachii. The high frequency of the protection trees designated by Chuncheon were Pinus densiflora, Ginko biloba, Zelkova serrata, Quercus species. It was thought that the diversification of street tree species, the selection of street trees suitable to each space, the generalization of use of native species, the appropriate pruning and proper fertilization, the control of pests and diseases, and the opinions of citizens concerning landscape plants were needed to improve the planting, use and maintenance of landscaping plants in Chuncheon.