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Therapeutic Anti-inflammatory Effect of Ginkgo Terpene on Arthritis due to Candida albicans  

Lee, Soon-Hyun (College of Pharmacy, Dongduk Women's University)
Lee, Jue-Hee (College of Pharmacy, Dongduk Women's University)
Han, Yong-Moon (College of Pharmacy, Dongduk Women's University)
Publication Information
YAKHAK HOEJI / v.49, no.2, 2005 , pp. 140-146 More about this Journal
Abstract
Candida albicans, a polymorphic fungus, causes systemic and local infections. Recent reports show that the fungus is a main etiological agent for the arthritis. For trea tment, antifungal drugs and/or rheumatoid drugs are used, but resistance and side effects limit application of the drugs. In search of new sources for treatment of the fungal arthritis, we choose Egb 761 (extract of Ginkgo leaves 761), one of the most popular over-the-counter herbal medicines. The Egb 761 contains two major ingredients such as terpene and flavonoid. In the present study, we examined if the terpene portion of Egb 761 had anti-inflammatory activity against C.albicans-caused arthritis. The terpene was extracted with combination of methanol and water from the Egb 761, followed by gel-permeation chromatography. Presence of terpene was determined by the Salkowski colorimetric method and HPLC analysis. For an animal model of inflammation induction, mice were given an emulsion form of C.albicans cell wall mixed with Complete Freund's Adjuvant (CFA) by footpad-injection. Results showed that intraperitoneal administration of the water-soluble portion that contained terpene and flavonoid reduced the inflammation. Whereas the terpene had anti-inflammatory activity, flavonoid portion had no such activity, For determination of possible mechanism of the activity, the terpene seemed to be suppression of nitric oxide (NO) production from LPS-treated macrophages. Taken together the Ginkgo terpene may have anti-inflammatory effect against C.albicans-caused arthritis, possibly by blocking NO production.
Keywords
Candia arthritis; EGb 761; Ginko terpene; anti-inflammation; NO; mice;
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1 Han, Y., Kozel, T. R., Zhang, M. X., MacGill, R. S., Carroll, M. C. and Cutler, J. E. : Complement is essential for protection by an IgM and an IgG3 monoclonal antibody against experimental, hematogenously disseminated candidiasis. J. Immunol. 167, 1550 (2001)   DOI
2 Li, W and Fitzloff, J. E ; Simultaneous determination of terpene lactones and flavonoid aglycones in Ginkgo biloba by highperformance liquid chromatography with evaporative light scattering detection. J. Pharm. Biomed. Anal. 30, 67 (2002)   DOI   ScienceOn
3 Maelennan, K. M., Darlington, C. L. and Smith, P. F. : The CNS effects of Ginkgo biloba extracts and ginkgolide B. Prog. Neurobiol. 67, 235 (2002)   DOI   ScienceOn
4 Lee, S. and Han, Y. : Isolation and analysis of ginkgolides compound from EGb 761. Dongduk Pharm. Res. 7, 39 (2003)
5 Lerch, K, Kalteis, T., Schubert, T., Lehn, N. and Grifka, J. : Prosthetic joint infection with osteomyelitis due to Candida albicans. Mycoses. 46, 462 (2003)   DOI   ScienceOn
6 Nissler, K., Pohlers, D., Huckel, M., Simon, J., Brauer, R. and Kinne, R. W. : Anti-CD4 monoclonal antibody treatment in acute and early chronic antigen induced arthritis: influence on macrophage activation. Ann. Rheum. Dis. 63, 1470 (2004).   DOI   ScienceOn
7 Kobuchi, H., Droy-Lefaix, J., Christen, Y. and Packer, L. : Ginkgo biloba extract (EGb 761) inhibitory effect on nitric oxide production in the machrophage cell line Raw 264.7. Biochem. Pharmacol. 53, 897 (1997)   DOI   ScienceOn
8 Hu, L., Chen, Z., Cheng, X. and Xie, Y. : Chemistry of ginkgolides: structure-activity relationship as PAF antagonists. Pure. Appl. Chem, 71, 1153 (1999)   DOI
9 Han, Y. and Cutler, J. E. : Antibody response that protects against disseminated candidiasis. Infect Immun. 63, 2714 (1995)
10 Braquet, P. and Hosford, D. : Ethnopharmacology and the development of natural PAF antagonists as therapeutic agents. J. Ethnopharmacol. 32, 135 (1991)   DOI   ScienceOn
11 Han, Y., Jin, B. S., Ko, S. K. and Lee, J. H. : Immunoactivity of ginsenosides Re and Rg1 enhances resistance of mice against experimental disseminated candidiasis. Nat. Prod. Sci. 10, 134 (2004)
12 Logani, S., Chen, M. C., Tran, T., Le, T. and Raffa, R. B. : Action of Ginkgo biloba related to potential for the treatment of conditions involving cerebral hypoxia. Life Sciences 1389 (2000)
13 Myers, L. K., Kang, A. H., Postlethwaite, A. E., Rosloniec, E. E, Morharn, S. G., Shlopov, B. V., Goorha, S. and Ballou, L. R. : The genetic ablation of cyclooxygenase 2 prevents the development of autoimmune arthritis. Arthritis Rheum. 43, 2687 (2000)   DOI   ScienceOn
14 Bauer, H. and Marker-Hermann, E. : Therapy with nonsteroidal anti-inflammatory drugs. Orthopade. 32, 1088 (2003)   DOI   ScienceOn
15 Lotito, A. P., Muscara, M. N., Kiss, M. H., Teixeira, S. A., Novaes, G. S., Laurindo, I. M., Silva, C. A. and Mello, S. B. ; Nitric oxide-derived species in synovial fluid from patients with juvenile idiopathic arthritis. Rheumatol. 31, 992 (2004)
16 Choi, I. S., Kim, S. J., Kim, B. Y., Joh, J. W., Kim, Y. I., Lee, S. K., Huh, W. S., Oh, H. Y., Kim, D. J, Kim, Y. G., Kim, M. K., Ko, Y. H. and Lee, B. B. : Candida polyarthritis in a renal transplant patient: case report of a patient successfully treated with amphotericin B. Transplant Proc. 32, 1963 (2000)
17 Zhao, H. W. and Li, X. Y. : Ginkgolide A, B, and huperzine A inhibit nitric oxide-induced neprotoxicity. Int. Immunopharmacol. 2, 1551 (2002)   DOI   ScienceOn
18 Kinne, R. W, Brauer; R., Stuhlmuller; B., Palombo-kinne, E. and Burmester, G.-R. : Macrophages in rheumatoid arthritis. Arthritis Res. 2, 189 (2000)   DOI   ScienceOn
19 Cuende, E., Barbadillo, C. E., Mazzucchelli, R., Isasi, C., Trujillo, A. and Andreu, J. L. : Candida arthritis in adult patients who are not intravenous drug addicts: report of three cases and review of the literature. Semin Arthritis Rheum. 22, 224 (1993)   DOI   ScienceOn
20 Beek, T. A. : Chemical analysis of Ginkgo biloba leaves and extracts. J. Chromato. A 967, 21 (2002)   DOI   ScienceOn
21 Han, Y., Ulrich, M. A. and Cutler, J. E. : Candida albicans mannan extract -protein conjugates induce a protective immune response against experimental candidiasis. J. Infect. Dis. 179, 1477 (1999)   DOI   ScienceOn
22 Lee, D. M. and Weinblatt, M. E. : Rheumatoid arthritis. Lancrt. 358, 903 (2001)   DOI   ScienceOn
23 Nishida, S. and Satoh, H. : Comparative vasodilating action among terpenoids and flavonoids contained in Ginkgo biloba extract. Clinica. Chimica Acta 339, 129 (2004)   DOI   ScienceOn
24 Azaceta, G., Olave. T., de los Martires, L. D., Delgado, C, Gutierrez, M. and Palomera, L. : Successful lipid-complexed amphotericin B treatment of Candida arthritis in a lymphoma patient. Rev. Rhum. Engl. 66, 434 (1999)
25 Felson, D. T., Anderson, J. J. and Meena, R. F. : Use of short-term efficacy/toxicity tradeoffs to select second-line drugs in rheumatoid arthritis: a metaanalysis of published clinical trials. Arthritis Rheum. 35, 1117 (1992)   DOI   ScienceOn
26 Turgut, B., Vural, O., Demir, M. and Kaldir, M. : Candida arthritis in a patient with chronic myelogenous leukemia (CML) in blastic transformation, unresponsive to fluconazole, but treated effectively with liposomal amphotericin B. Ann. Hematol. 81, 529 (2002)   DOI   ScienceOn
27 DeFeudis, F. Y. : Ginkgo biloba Extract (EGb 761) : Pharmacological activities and Clinical Application. Elesevier 97, 135 (1991)