• 대한한의학회지
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• 제20권2호
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• pp.88-97
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• 1999

To characterize the effects of Gilgyung-Tang(GGT) on cellular proliferation and viability of normal lung fibroblast cells, we examined the cell cycle progression and cell cycle-related gene expression in T3891 using a flow cytometry and a quantitative RT-PCR analysis. 1. The significant surpression effect of cellular proliferations of GGT was observed in proportion to a certain concentration and time. 2. GGT was identified to induce apoptotic death of damaged cells by treatment with a DNA-damage agent and etoposide, while it stimulated the recovery of cellular viability of normal cells. 3 The significant reductions of mRNA expression of PCAN, c-Fos treated by GGT were observed. 4. The significant inductions of mRNA expression of p53, CDKN1. Gadd45 treated by GGT were observed. 5. The apoptosis caused by the reduction of Bcl-2 genes was significant and the Bax genes were increased. but the amount of Fas genes were not changed. These results strongly suggest that GGT triggers arrest of the cell cycle at G1 phase, and thus causes an inhibition of cellular proliferation of human normal lung cells through the transcriptional up-regulation of cell cycle inhibitory genes and down-regulation of induction of cell cycle stimulating genes respectably.

• 대한한방내과학회지
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• 제37권1호
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• pp.16-25
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• 2016

Objectives: Gilgyung-tang (GGT) has been used as one of the main multi-herb formulas to treat “Peo-ong” (lung abscess). In this study, we investigated the inhibitory effects of water extracts of GGT on cell migration and invasion, two critical cellular processes that are often deregulated during metastasis, in human bladder cancer 5637 cells.Methods: Effects on cell viability were quantified using an MTT assay. To analyze the anti-metastatic effects, we conducted a wound healing migration assay, an in vitro invasiveness assay, and a measurement of the transepithelial electrical resistance (TER). The expression of protein and mRNA were measured by Western blotting and real-time polymerase chain reaction (RT-PCR), respectively.Results: GGT markedly inhibited the cell motility and invasiveness of 5637 cells within the concentration range that was not cytotoxic. The inhibitory effects of GGT on cell invasiveness were associated with tightening of the tight junctions (TJs), which was demonstrated by an increase in the TER. The RT-PCR and Western blotting results indicated that GGT decreased the levels of claudin proteins. GGT also inhibited the activity and expression of matrix metalloproteinase (MMP)-2 and -9 and simultaneously increased the levels of tissue inhibitor of metalloproteinase-1 and -2.Conclusions: Our findings suggest that GGT reduces both the migration and the invasion of 5637 cells by modulating the activity of TJs and MMPs.

• 대한예방한의학회지
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• 제20권2호
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• pp.69-75
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• 2016

Objectives : The aim of this study is to introduce the Korean medicine respiratory management program in public health center and to analyze the effects of that program. Methods : The respiratory management program was targeted on 22 soldiers during March 2016. The interventions of this program were herbal medicines (Gilgyung-tang and so on), aromatherapy, peppermint tea, fine dust mask, and health education. Data were collected by retrospective reviewing medical chart and analyzed using SPSS 18.0. Results : Total symptoms scores were measured by 4-point likert scale. Total score was $5.50{\pm}4.848$ at 1st week and $3.27{\pm}3.058$ at 4th week (p=0.008). The severity of respiratory discomfort reduced by $-1.14{\pm}2.46$ compared with last year. The satisfaction score was $7.82{\pm}4.16$. The most hoping program was muscular skeletal disease management program. Conclusions : This study suggests that Korean medical world should prepare countermeasures to cope with diseases caused by air pollution.

• 대한한의학방제학회지
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• 제24권4호
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• pp.271-282
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• 2016

Objective : In this study, we compared the antioxidant and anticancer properties of four multi-herbal formulas which were recorded in 'Dongeuibogam': Gilgyung-tang (GGT), Mokdanpi-tang (MDPT), Samso-eum (SSE), Samchulbobi-tang (SCBBT). Methods : We checked antioxidant properties of four multi-herbal formula through total phenolic content, radical scavenging activities, protective effects on genomic DNA oxidation. To investigate anticancer effects, we conducted MTT assay and analyzed morphologic change in A549 non-small lung cancer cells. Results : Total phenolic contents of four multi-herbal formulas were in a rich order of MDPT > SSE > GGT > SCBBT. Especially, MDPT revealed the highest activity than others in all antioxidant experiments. Our results indicated that treatment of those multi-herbal formulas induced growth retardation in A549 cells and MDPT also showed the highest anticancer effect ($IC_{50}=1.374mg/ml$) among them. Conclusions : Our data suggested that MDPT would be a powerful ingredient for lung cancer treatment.

• 생명과학회지
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• 제25권4호
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• pp.406-415
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• 2015

본 연구에서, 우리는 동의보감에서 옹저에 대한 한약재 처방전인 길경탕(GGT), 대황목단탕(DHMDT), 사간탕(SGT), 소청룡탕(SCRT), 시호청간탕(SHCGT), 십육미류기음(SYMYKE) 과 황흑산(HHS)에 대한 항산화능과 항암과 항균활성에 대한 생리활성에 대한 연구를 수행하였다. 총 페놀 함량은 길경탕 ＜ 십육미류기음 ＜ 소청룡탕 ＜ 시호청간탕 ＜ 대황목단탕 ＜ 사간탕 ＜ 황흑산의 순서대로 함량이 풍부하였다. 이들 중 황흑산은 SOD 유사활성, 환원력과 DPPH 라디칼과 ABTS 라디칼의 소거능이 가장 뛰어났다. 흥미롭게도, 이들 시료의 총페놀 함량과 그들의 항산화능과의 상관도는 높게 나타났다. 모든 시료에서 인간대장세포인 HCT-116에서 항암활성을 나타냈으며, 특히 황흑산은 가장 낮은 활성을 나타낸 길경탕보다도 7배 높은 항암활성을 보였다. 또한, 대장균인 Escherichia coli와 위염을 발생시키는 Helicobacter pylori 균에 대한 항균 활성을 시행한 결과 사간탕, 소청룡탕, 십육미류기음과 황흑산에서 항균활성이 보였다. 결과를 종합하여 볼 때, 한약재 처방전은 항산화와 생리활성물질의 소재로서의 가치가 있음을 알 수 있었다.