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http://dx.doi.org/10.5352/JLS.2015.25.4.406

Antioxidant, Antimicrobial and Anticancer Properties of Seven Traditional Herb-combined Remedies  

Lee, Moon Hee (Department of Biochemistry, Dongeui University College of Korean Medicine)
Lee, Jae-wang (Department of Molecular biology, Dongeui University College of Nature Science and Human Ecology, Dongeui University)
Park, Cheol (Department of Molecular biology, Dongeui University College of Nature Science and Human Ecology, Dongeui University)
Han, Min Ho (Department of Biochemistry, Dongeui University College of Korean Medicine)
Hong, Su Hyun (Department of Biochemistry, Dongeui University College of Korean Medicine)
Choi, Yung Hyun (Department of Biochemistry, Dongeui University College of Korean Medicine)
Publication Information
Journal of Life Science / v.25, no.4, 2015 , pp. 406-415 More about this Journal
Abstract
In this study, we investigated the antioxidant activities and compared other physiological properties including anti-cancer and antimicrobial effects of several traditional Korean herb-combined remedies such as Gilgyung-tang (GGT), Daihwangmokdan-tang (DHMDT), Sagan-tang (SGT), Socheonryongtang (SCRT), Sihocheonggan-tang (SHCGT), Sipyukmiyuki-eum (SYMYKE) and Hwangheuk-san (HHS), which were recorded in “Dong-eui-bo-gam” for “Ongjeo”. Total phenolic contents of the herb medicines were in a rich order of GGT ＜ SYMYKE ＜ SCRT ＜ SHCGT ＜ DHMDT ＜ SGT ＜ HHS. Among them, HHS appeared highest in superoxide dismutase-like activity, ferric reducing antioxidant power, scavenging of 2,2’-diphenyl-1-picrylhydrazyl, and 2,2’-azinobis(3-ethylbenzothiazoline-6-sulfonate) cationic radical activity. Interestingly, there was a positive relationship between their total phenolic contents and their antioxidant activities. Although all of them showed anti-proliferative activities in human colon cancer HCT-116 cells, HHS was seven times higher than GGT. Antimicrobial activities against Escherichia coli and Helicobacter pylori were revealed only on SGT, SCRT, SYMYKE and HHS. Taken together, these findings reveal the potential use of traditional Korean herbal formulas as functional ingredients in antioxidant and physiological materials.
Keywords
Anticancer activity; antimicrobial activity; antioxidant activity; traditional herb-combined remedies;
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1 Emerit, J., Edeas, M. and Bricaire, F. 2004. Neurodegenerative diseases and oxidative stress. Biomed. Pharmacother. 58, 39-46.   DOI
2 Fridovich, I. 1998. Oxygen toxicity, a radical explanation. J. Exp. Biol. 201, 1203-1209
3 Gordon, M. F. 1990. The mechanism of antioxidant action in vitro, pp. 1-18. In: Hudson, B. J. F. (ed.), In Food Antioxidants. Elsevier Applied Food Science publishers, London, UK.
4 Han, I. S. 2013. Anti-inflammatory effect of sihocheonggantang extraction. Master dissertation, Sangji University, Seoul, Korea.
5 Han, J. H. and Lee, S. Y. 2011. Comparing medical efficacy of Socheongyong-tang with lactic acid bacteria fermented Socheongyong-tang. Kor. J. Ori. Med. Physiol. Pathol. 5. 246-256.
6 Hansen, M. B., Nielsen, S. E. and Berg, K. 1989. Re-examination and further development of a precise and rapid dye method for measuring cell growth/cell kill. J. Immunol. Methods 119, 203-210.   DOI
7 Heo, J. Donguibogam, 1999. pp. 1433-1439. In: Kim, K. J. (ed.), Beopinmunhwasa publishers, Seoul, Korea.
8 Kim, B. J. 2000. Antimicrobial activities of the extracts of Platycodon glandiforum A. De. Candolle on the growth of Listeria monocytogenes. Kor. J. Soc. Food Sci. 16, 379-384.
9 Hollman, P. C. H. 2001. Evidence for health effects of plant phenols: localor systemic effects? J. Sci. Food Agric. 81, 842-852.   DOI
10 Hong, S. H., Park, C., Han, M. H., Kim, H. J., Lee, M. H. and Choi, Y. H. 2014. Effects of Platycodon grandiflorum on the induction of autophagy and apoptosis in HCT-116 human colon cancer cells. J. Life Sci. 24, 1244-1251.   DOI
11 Jang, M., Seo, J., Lee, J. H. and Kim, G. H. 2010. Antibacterial activities of essential oil from Zanthoxlum schinifolium against foodborne pathogens. Kor. J. Food Cook Sci. 26, 206-213.
12 Kim, C. J., Cho, S. K., Choi, C. S. and Choi, Y. S. 1990. Hepatoprotective activities of traditional Korean Herbal prescritions. Kor. J. Pharmacongn. 21, 223-224.
13 Kim, D. O., Jeong, S. W. and Lee, C. Y. 2003. Antioxidant capacity of phenolic phytochemicals from various cultivars of plums. Food Chem. 81, 321-326.   DOI
14 Kim, H. W., Lim, C. Y., Kim, B. Y. and Cho, S. I. 2014. So-Cheong-Ryong-Tang, a herbal medicine, modulates inflammatory cell infiltration and prevents airway remodeling via regulation of interleukin-17 and GM-CSF in allergic asthmain mice. Pharmacogn. Mag. 10, 506-511.   DOI
15 Kim, S. Y., Lee, E. B. and Jeong, E. J. 2009. Anti-Inflammatory action of the fractions of Platycodi radix. Kor. J. Food Nutr. 22, 618-624.
16 Kim, Y. K., Ha, H. K., Lee, J. H. and Kim, C. S. 2002. COX-2 inhibition and anti-inflammatory effects of traditional herbal medicine. Kor. J. Ori. Med. 8, 65-74.
17 Lim, H. R. and Shin, S. W. 2010. Effects of the essential oil components from Ligusticum chuanxiong on proinflammatory mediators of Raw 264.7 macrophage cells. Nat. Prod. Sci. 16, 259-264.
18 Lee, I. S., Choi, M. C. and Moon, H. Y. 2000. Effect of Platycodon grandiflorum A. DC extract on the bronchus diseases bacteria. Kor. J. Biotech. Bioeng. 15, 162-166.
19 Lee, J. Y., Lee, J. M., Lee, C. H., Cho, J. H., Jang, J. B. and Lee, K. S. 2010. Antitumor and immunomodulatory activities of Platycodon grandiflorum. J. Ori. Obes. Gynecol. 23.10-19.
20 Mo, J. H., Lee, S. E., Wee, J. H., Lee, J. E., Rhee, C. S., Lee, C. H. and Kim, D. Y. 2013. Anti-allergic effects of So-Cheong- Ryong-Tang, a traditional Korean herbal medicine, in an allergic rhinitis mouse model. Eur. Arch. Otorhinolaryngol. 270, 923-30.   DOI
21 Paik, S. B., Kyung, S. H., Kim, J. J. and Oh, Y. S. 1996. Effect of bioactive substance extracted from Rheum undulatum on control of cucumber powdery mildew. Kor. J. Plant Pathol. 12. 85-90.
22 Park, U. Y., Chang, D. S. and Cho, H. R. 1992. Screenging of antimicrobial activity for medicinal herb extracts. J. Kor. Soc. Food Nurt. 21. 91-96
23 Robards, K., Prernzler, P. D., Tucker, G. and Swatsitang, P. and Glover, W. 1999. Phenolic compounds and their role in oxidative processes in fruits. Food Chem. 66, 401-436.   DOI
24 Roberta, R., Nicoletta, P., Anna, P., Ananth, P., Min., Y. and Catherine, R. E. 1999. Antioxidant activity applying an improved ABTS radical cation decolorization assay. J. Free Rad. Biol. Med. 26, 1231-1237.   DOI
25 Ryu, J. H., Jang, J. H., Lee, S. Y., Lee, H. J. and Han, Y. N. 1998. Inhibitors of nitric oxide synthesis from ginseng in activated macrophages. J. Ginseng Res. 22, 181-187.
26 Singleton, V. L. and Rossi, J. A. 1965. Colorimetry of total phenolics with phosphomolybdic–phosphotungstic acid reagents. Am. J. Enol. Vitic. 16, 144-158.
27 Wolfenden, B. S. and Willson, R. L. 1982. Radical-cations as reference chromogens in kinetic studies of one-electron transfer reactions: pulse radiolysis studies of 2, 2’-azinobis-(3-ethylbenzthiazoline-6-sulphonate). J. Chem. Soc. Perkin. Trans. 2, 805-812.
28 Liu, L., Sun, Y., Laura, T., Liang, X., Ye, H. and Zeng, X. 2009. Determination of polyphenolic content and antioxidant activity of kudingcha made from Ilex kudingcha C. J. Tseng. Food Chem. 112, 35-41.   DOI
29 Li, Z., Li, L. J., Sun Y. and Li, J. 2007. Identification of natural compounds with anti-hepatitis B virus activity from Rheum palmatum L. ethanol extract. Chemotherapy 53, 320-326.   DOI
30 Lim, Y., Park, K. R., Lee, D. U., Kim, Y. S. and Kim, H. P. 2008. Effects of the constituents of gardenia fructus on prostaglandin and NO production. Biomolecules Therapeutics 16, 82-86.   DOI
31 Luximon-Ramma, A., Bahorun, T., Soobrattee, A. M. and Aruoma, O. I. 2005. Antioxidant activities of phenolic, proanthocyanidin and flavonoid components in extracts of Acacia fistula. J. Agric. Food Chem. 50, 5042-5047.
32 Markland, S. and Markland, G. 1974. Involvement of superoxide radical in the autoxidation of pyrogallol and convenient assay for superoxide dismutase. Eur. J. Biochem. 47, 469-472.   DOI
33 Mello, L. D., Hernandez, S., Marrazza, G., Mascini, M. and Kubota, L. T. 2006. Investigations of the antioxidant properties of plant extracts using a DNA-electrochemical biosensor. Biosens Bioelectron 21, 1374-1382.   DOI
34 Park, K. H., Kim, S. Y. and Chae, H. J. 2007. Selection of oriental medicinal plants for screeninf of anticancer agents. Kor. J. Biotechnol. Bioeng. 22. 139-145.
35 Milne, L., Nicotera, P., Orrenius, S. and Burkitt, M. 1993. Effects of glutathione and chelating agents on copper-mediated DNA oxidation: Prooxidant and antioxidant properties of glutathione. Arch. Biochem. Biophys. 304, 102-109.   DOI
36 Xu, Q., Mori, H., Sakamoto, O., Uesugi, Y. and Koda, A. 1989. Immunological mechanisms of antitumor activity of some kinds of crude drugs on tumor necrosis factor production. Int. J. Immunopharmacol. 11, 607-613.   DOI
37 Shung, S. H., Moon, Y. J., Kim, S. G., Kim, K. Y., Lee, K. T., Kim, H. K. and Whang, Y. K. 2008. Isolation of flavonoids from Carthami Flos and their antioxidative activity. Yakhak Hoeji 52, 241-251.
38 Valko, M., Rhodes, C. J., Moncol, J., Izakovic, M. and Mazur, M. 2006. Free radicals, metals and antioxidants in oxidative stress-induced cancer. Chem. Biol. Interact 10, 1-40.
39 Vaya, J., Belinky, P. A. and Aviram, M. 1997. Antioxidant constituents from licorice roots: Isolation, structure elucidation and antioxidative capacity toward LDL oxidation. Free Radic. Biol. Med. 23, 302-313.   DOI
40 Yang, Z. C., Wang, B. C., Yang, X. S., Wang Q. and Ran, L. 2005. The synergistic activity of antibiotics combined with eight traditional Chinese medicines against two different strains of Staphylococcus aureus. Colloids Surf. B. Biointerfaces 25, 79-81.
41 Yoon, S. B., Han, H. S. and Lee, Y. J. 2011. Effect of scutellariae radix extract on the proinflammatory mediators in Raw 7 cells induced by LPS. Kor. J. Herbology 26, 75-81.
42 Yoon, T. S., Cheon, M. S., Kim, S. J., Lee A. Y., Moon, B. C., Chun, J. M., Choo, B. K. and Kim, H. K. 2010. Evaluation of solvent extraction on the anti-inflammatory efficacy of Glycyrrhiza uralensis. Kor. J. Med. Crop. Sci. 18, 28-33.
43 Choi, S. W., Kan, H., Sin, B. S, Kim, S. H. Choi, S. H. and Ahn, K. S. 2009. Effect of bupeuri raix on inflammatory cytokine secretion to HMC and mouse immune cells. Kor. J. Ori. Med. Physiol. Pathol. 23, 150-157.
44 Yoon, Y. H., Kim, S. B., Kang, O. H., Mun, S. H., Seo, Y. S., Yang, D. W., Kang, D. H., Wi, G., Lim, J. S., Kim, M. R., Kwak, N. W., Komg, R. and Kwon, D. Y. 2014. The study of anti-inflammatory effect of Suryeon-hwan water extract in RAW 264.7 cells. Kor. J. Herbology 29. 125-135.   DOI
45 Bauer, A. W., Kirby, W. M., Sherris, J. C and Turck, M. 1966. Antibiotic susceptibility testing by a standardized single disk method. Am. J. Clin. Pathol. 45, 493-496.
46 Benzie, I. F. F. and Strain, J. J. 1996. The ferric reducing ability of plasma (FRAP) as a measure of “antioxidant power”: the FRAP assay. Anal. Biochem. 239, 70-76.   DOI
47 Blios, M. S. 1958. Antioxidant determination by the use of a stable free radical. Nature 26, 1158-1200.
48 Cataldi, A. 2010. Cell responses to oxidative stressors. Curr. Pharm. Des. 16, 1387-1395.   DOI
49 Dröge, W. 2002. Free radicals in the physiological control of cell function. Physiol. Rev. 82, 47-95.   DOI
50 Choi, Y., Kim, M., Shin, J. J., Park, J. M. and Lee, J. 2003. The antioxidant activities of the some commercial teas. J. Kor. Soc. Food Sci. Nutr. 32, 723-727.   DOI
51 Chu, C. K., Jung, S. K., Jung, H. J. and Kim, J. J. 2010. Research on anti-inflammatory effects of forsythiae fructus. Kor. J. Ori. Int. Med. 31, 242-253.
52 Chung, S. H., Moon, Y. J., Kim, S. G., Kim, K. Y., Lee, K. T., Kim, H. K. and Whang, H. K. 2008. Isolation of flavonoids from carthami flos and their antioxidative activity. Yakhak Hoeji 52, 241-251.