Browse > Article
http://dx.doi.org/10.14374/HFS.2016.24.4.271

Comparison of Four Multi-herbal Formulas in Antioxidant and Anticancer Activities  

Kim, Hong Jae (Department of Pharmacy, College of Pharmacy, Pusan National University)
Choi, Eun Ok (Anti-Aging Research Center, Dong-eui University)
Kim, Min Young (Department of Biological Sciences, College of Natural Sciences, Pusan National University)
Son, Da Hee (Department of Biochemistry, Dong-eui University College of Korean Medicine)
Jeong, Jin-Woo (Anti-Aging Research Center, Dong-eui University)
Lee, Sang-hyup (Department of Korean Medical Classics, Dong-eui University College of Korean Medicine)
Park, Cheol (Department of Molecular Biology, College of Natural Sciences and Human Ecology, Dong-eui University)
Choi, Yung Hyun (Anti-Aging Research Center, Dong-eui University)
Hong, Su Hyun (Department of Biochemistry, Dong-eui University College of Korean Medicine)
Publication Information
Herbal Formula Science / v.24, no.4, 2016 , pp. 271-282 More about this Journal
Abstract
Objective : In this study, we compared the antioxidant and anticancer properties of four multi-herbal formulas which were recorded in 'Dongeuibogam': Gilgyung-tang (GGT), Mokdanpi-tang (MDPT), Samso-eum (SSE), Samchulbobi-tang (SCBBT). Methods : We checked antioxidant properties of four multi-herbal formula through total phenolic content, radical scavenging activities, protective effects on genomic DNA oxidation. To investigate anticancer effects, we conducted MTT assay and analyzed morphologic change in A549 non-small lung cancer cells. Results : Total phenolic contents of four multi-herbal formulas were in a rich order of MDPT > SSE > GGT > SCBBT. Especially, MDPT revealed the highest activity than others in all antioxidant experiments. Our results indicated that treatment of those multi-herbal formulas induced growth retardation in A549 cells and MDPT also showed the highest anticancer effect ($IC_{50}=1.374mg/ml$) among them. Conclusions : Our data suggested that MDPT would be a powerful ingredient for lung cancer treatment.
Keywords
Antioxidant activity; anticancer activity; multi-herbal formula; Mokdanpi-tang;
Citations & Related Records
Times Cited By KSCI : 10  (Citation Analysis)
연도 인용수 순위
1 Lee MH, Lee JW, Park C, Han MH, Hong SH, Choi, YH. Antioxidant, Antimicrobial and Anticancer Properties of Seven Traditional Herb-combined Remedies. J Life Sci. 2015;25(4):406-415.   DOI
2 Liu L, Sun Y, Laura T, Liang X, Ye H, Zeng X. Determination of polyphenolic content and antioxidant activity of kudingcha made from Ilex kudingcha C J Tseng. Food Chem 2009;112:35-41.   DOI
3 Hong SM, Cho HD, Kim JH, Lee JY, Park JM, Seo KI. Anti-oxidant and Anti-proliferative Effects of Water Extract Mixture of Cordyceps Militaris and Allium Tuberosum. J Life Sci. 2016;26(7):805-811.   DOI
4 Klaunig JE, Kamendulis LM. The role of oxidative stress in carcinogenesis. Annu Rev Pharmacol Toxicol. 2004;44:239-67.   DOI
5 Zhao R, Chen M, Jiang Z, Zhao F, Xi B, Zhang X, Fu H, Zhou K. Platycodin-D Induced Autophagy in Non-Small Cell Lung Cancer Cells via PI3K/Akt/mTOR and MAPK Signaling Pathways. J Cancer. 2015:6(7):623-31.   DOI
6 Park YS, Kim SH, Park SK, Park BJ, Kim YT, Lee SM et al. Costs for 5-year lung cancer survivors in a tertiary care hospital in South Korea. Lung Cancer. 2010;68(2):299-304.   DOI
7 Hsiao WL, Liu L. The role of traditional Chinese herbal medicines in cancer therapy-from TCM theory to mechanistic insights. Planta Med. 2010;76(11):1118-31.   DOI
8 Chen Y, Zhu J, Zhang W. Antitumor effect of traditional Chinese herbal medicines against lung cancer. Anticancer Drugs. 2014;25(9):983-91.   DOI
9 Gao L, Li Q, Jiang M, Liu C, Song Z, Bao X, et al. Combined therapy of percutaneous cryoablation and traditional Chinese medicine can be a promising strategy for elderly or advanced lung cancer patients based on a retrospective clinical study. Cryobiology. 2014;69(1):174-7.   DOI
10 Lee DH, Seong S, Kim SS, Han JB. A Case of Stage IV Non-Small Cell Lung Cancer Treated with Korean Medicine Therapy Alone. Case Rep Oncol. 2013;6(3):574-8.   DOI
11 Tekiner-Gulbas B, Westwell AD, Suzen S. Oxidative stress in carcinogenesis: new synthetic compounds with dual effects upon free radicals and cancer. Curr Med Chem. 2013;20(36):4451-9.   DOI
12 Luo J, Shen L, Zheng D. Association between vitamin C intake and lung cancer: a dose-response meta-analysis. Sci Rep. 2014;4:6161.
13 Chen G, Wang J, Hong X, Chai Z, Li Q. Dietary vitamin E intake could reduce the risk of lung cancer: evidence from a meta-analysis. Int J Clin Exp Med. 2015;8(4):6631-7.
14 Lee KH, Kim SH, Song HJ. Study on Antitumor Effect and Immune Response of Kikyungtang and Kamikilkyungtang. Korean J Oriental Medical Pathology. 1996;10(1):105-119.
15 Kang PG, Park DI, Choi WC, Heon JC. Effects of Several Medicine Herb Prescriptions on Lung Carcinoma Cells. J Int Korean Med. 2000;21(4):621-631.
16 Hang SH, Kim JW, Kim BC, Woo SH, Son JH, Lee JH, et al., Bronchiectasis and Pneumonia in an Elderly Patient Treated through Paeong(肺癰). J Int Korean Med. 2005;26(3):626-633.
17 Park JY, Jang SH. Epidemiology of Lung Cancer in Korea: Recent Trends. Tuberc Respir Dis (Seoul). 2016;79(2):58-69.   DOI
18 Molina JR, Yang P, Cassivi SD, Schild SE, Adjei AA. Non-small cell lung cancer: epidemiology, risk factors, treatment, and survivorship. Mayo Clin Proc. 2008:83(5);584-94.   DOI
19 Lee K, Kim YS, Son CG, Cho JH, Yoo HS, Lee J, et al. Combination Therapy of Gefitinib and Korean Herbal Medicines Could be a Beneficial Option for Patients with Non-Small-Cell Lung Cancer. J Pharmacopuncture. 2016;19(3):259-263.   DOI
20 Khan M, Maryam A, Zhang H, Mehmood T, Ma T. Killing cancer with platycodin D through multiple mechanisms. J Cell Mol Med. 2016;20(3): 389-402.   DOI
21 Heo J. Donguibogam, Seoul: Beopinmunhwasa publishers. 1999:1433-1437.
22 Kim JH, Lee DJ, Yun SJ, Ahn SW, Kim YK. Building the Database with Herbal formulas Based on the Korean Medical Classics. Herbal Formula Science 2015;23(2):209-224.   DOI
23 Han JS, Jwon HS, Jung SG, Lee HK. Lieterature reviews on lung stagnation, lung abscess, cellulitis in the lung and lung cancer based on their causes and symptoms. J Korean Oriental Med. 1978;15(1):26-35.
24 Hong SH, Park C, Han MH, Kim HJ, Lee MH, Choi YH. Effects of Platycodon grandiflorum on the Induction of Autophagy and Apoptosis in HCT-116 Human Colon Cancer Cells. J Life Sci. 2014;24(11):1244-1251.   DOI
25 Hong SH, Han MH, Park C, Park SE, Hong SH, Choi TY. Induction of Autophagy and Apoptosis by the Roots of Platycodon grandiflorum on NCI-H460 Human Non-small Lung Carconoma Cells. J Int Korean Med. 2014:35(3);317-331.
26 Kim SC, Kwon HS, Kim CH, Kim HS, Lee CY, Cho SJ. Comparison of Antioxidant Activities of Pileus and Stipe from White Beech Mushrooms(Hypsizygus marmoreus). J Life Sci 2016;26(8);928-935.   DOI
27 Li X, Wu X and Huang L. Correlation between antioxidant activities and phenolic contents of radix Angelicae sinensis (Danggui). Molecules. 2009;14:5349-5361.   DOI
28 Re R, Pellegrini N, Proteggente A, Pannala A, Yang M and Rice-Evans C. Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Radic Biol Med. 1999:26;231-1237.
29 Benzie IFF and Strain JJ. The ferric reducing ability of plasma (FRAP) as a measure of "antioxidant power": the FRAP assay. Anal Biochem. 1996;239:70-76.   DOI
30 Blios MS. Antioxidant determination by the use of a stable free radical. Nature. 1958;26:1158-1200.
31 Milne L, Nicotera P, Orrenius S and Burkitt M. Effects of glutathione and chelating agents on copper-mediated DNA oxidation: Prooxidant and antioxidant properties of glutathione. Arch. Biochem. Biophys. 1993;304:102-109.   DOI
32 Hyun JM, Park KJ, Kim SS, Park SM, Lee Yj, An Hj. Antioxidant and Anti-inflammatory Effects of Solvent Fractions from the Peel of the Native Jeju Citrus 'Hongkyool' and 'Pyunkyool'. J Life Sci. 2015;25(10):1132-1138.   DOI
33 Filaire E, Dupuis C, Galvaing G, Aubreton S, Laurent H, Richard R, et al. Lung cancer:what are the links with oxidative stress, physical activity and nutrition. Lung Cancer. 2013;82(3):383-9.   DOI
34 Lawless MW, O'Byrne KJ, Gray SG. Targeting oxidative stress in cancer. Expert Opin Ther Targets. 2010;14(11):1225-45.   DOI
35 Risom L, Moller P, Loft S. Oxidative stressi-nduced DNA damage by particulate air pollution. Mutat Res. 2005;592(1-2):119-37.   DOI
36 Mathew GE, Mathew B, Gokul S, Krishna R, Farisa MP. Determination of in vitro free radical scavenging and antiproliferative effect of Pennisetum alopecuroides on cultured A549 human lung cancer cells. Anc Sci Life. 2015;34(3):175-8.   DOI