• Reproductive and Developmental Biology
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• v.39 no.4
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• pp.127-132
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• 2015

To overcome the hyperacute immune rejection during pig-to-non-human primates xenotranasplantation, we have produced and bred ${\alpha}$-1,3-galactosyltransferase knock-out ($GalT^{-/-}$) pigs. In this study, the somatic cells and tissues from the $GalT^{-/-}$ pigs were characterized by an analysis of the expression of Gal${\alpha}$-1,3-Gal (${\alpha}-Gal$) epitope. Briefly, ear fibroblast cell lines of 19 homozygous $GalT^{-/-}$ pigs were established and cryopreserved. The expression of ${\alpha}-Gal$ epitope in the cells was measured by fluorescence activated cell sorter (FACS) analysis using BS-I-B4 lectin. Also, the homozygous ($GalT^{-/-}$) cells and tissues samples were immunostained with BS-I-B4 lectin for analysis of ${\alpha}-Gal$ epitope expression. The results showed that the expression of ${\alpha}-Gal$ epitope in $GalT^{-/-}$ cells (0.2 %) were significantly (p<0.05) down-regulated to the range of cynomolgus monkey fibroblast (0.2 %) cells compared to heterozygous ($GalT^{-/+}$) (9.3 %) and wild type ($GalT^{+/+}$) (93.7 %) fibroblast cells. In the immunostaining results, while the expression of ${\alpha}-Gal$ epitope was detected a partly in $GalT^{-/+}$ cells and mostly in $GalT^{+/+}$ cells, it was almost not detected in the $GalT^{-/-}$ cells. Also, immunostaining results from various tissues of the $GalT^{-/-}$ pig showed that the expression of ${\alpha}-Gal$ epitope was not detectable, whereas various tissues from $GalT^{+/+}$ pig showed a strong expression of ${\alpha}-Gal$ epitope. Our results demonstrated that ${\alpha}-Gal$ epitope expressions from $GalT^{-/-}$ pigs were successfully knocked out to prevent hyperacute immune rejection for further study of xenotransplantation.

• Reproductive and Developmental Biology
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• v.32 no.2
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• pp.117-121
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• 2008

Our previous study showed that transgenic (TG) pigs harboring human EPO (hEPO) gene have been shown to have reproductive disorders, including low pregnancy rates, irregular estrus cycle and low little size. To investigate these reasons, we assessed estrus behavior (standing response) and plasma $17{\beta}$-estradiol ($E_2$) level, which partly reflect reproductive function, during the estrus cycles after synchronization and superovulation by hormone treatments. Then, we analysed blood composition and expression of hEPO gene in TG pigs. Pigs were injected with PG600. After 10 days, pigs were fed with Regumate porcine for 6 days. Blood samples were collected from jugular vein. Analysis of blood composition and $E_2$ level were measured by Hemavet 950 and $E_2$ ELISA kit, respectively. And, the expression of hEPO gene in reproductive organs was quantitated by real-time RT-PCR. The percentage of estrus behavior in TG was significantly decreased. Hematocrit (HCT), hemoglobin (Hb) concentration and red blood cell (RBC) number were significantly higher in TG than wild type (WT). On the other hand, high expression of hEPO gene in TG was observed in the mammary gland as well as in the uterus. Moreover, plasma $E_2$ level was significantly higher in TG than WT. These results suggest that nonspecific expression of hEPO gene in the other organs of TG may affect blood composition and plasma $E_2$ level, thereby causing reproductive disorders.

• Journal of Korean Physical Therapy Science
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• v.4 no.2
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• pp.421-432
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• 1997

To find out the actual status of treatment of physically handicapped who were stayed of home, 320 physically handicapped persons were selected among total 6,264 physically handicapped registered in Taejon city, surveyed from January 1st to March 30, 1997. Following are the results ana lysed of collected data from 201 samped persons. 1. For the cause of physically handicapped, 36.3% was congenital, 26.9% was cerebralvascular acciednt(CVA). and 14.9% was infectious diseases and others in that orders. The must frequest cause of below 20 years age group was congenital cause, but that of $20{\sim}30$ years age group and $40{\sim}50$ years age group were accident and CVA repectively. 30.0% of respondents caused by infection and others earned household living expenses. Household living expenses were higher among the respondents caused by congenital cause but those were lowest in the accidental couse group. Handicap durations were longer in the accidental cause group and infections and others group than the congenital cause group and CVA group. 2. 54.7% of studied handicapped were under treatment. But 36.3% of them were stopped treatment, and 8.9 % of them answered not treated. Most handicapped, coused by congenital and CAV, were under treatment, but most of them caused by accidental and others were stopped treatment. 3. For the medical facilities, 54.5% of them utilized welfare facility, but 25.5% utilized general hospital and 20.0% of them utilized oriental medical hospital and health centers. Congenital cause group frequently selected welfare facility for the treatment facility. But accidental cause group frequently seleced general hospitals and CVA gruop frequently selected oriental medical hospital and others. The medical cost of welfare facility, oriental medical hospitals and others were lower than that of general hospitals. 4. The proportions of under treatment were higher among yonger age higher monthly living expenses group shorter handicapped duration groups, congenital cause group and handicap grade II group. But that of stop treatment were higher among congenital cause group, infectious and others cause group, and handicap grade I & then III group. 5. For the feelings satisfacion about medical treatment, only 35.3% of under treated group responed satisfation. 55.5% of them responded so-so, but 9.1 % responed dissatisfaction. The proportion of satisfaction was higher among shorter handicap duration group.

• Journal of the Korean Society for Marine Environment & Energy
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• v.3 no.3
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• pp.13-23
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• 2000

The distributions of dissolved inorganic nutrient contents were investigated along transection line J (30° N) in the eastern East China Sea in December 1993 and August 1994, respectively. The concentrations of nitrate and silicate in the Kuroshio Surface Water (KSW) with high temperature and high salinity were low below 2μM and 5μM, respectively. However, these were increased sharply with depth and ranged from 20 to 40, 45 to 100μM, respectively, in the Kuroshio Intermediate Water (KIW). The relationship between temperature and nutrients suggests that Kuroshio Intermediate Water with rich nutrients were intruded into the bottom water of the outer continental shelf in the East China Sea. The bottom water of the outer continental shelf was made of two end-members mixing; nutrient depleted warm water and nutrient enriched cold water. Based on temperature, salinity and silicate concentration, the nutrients in the bottom water of the outer continental shelf suggusted to be supplied through the vertical mixing of Kuroshio subsurface water in the depth range of 100～400m. Upwelled nutrient rich water appears to be a important source of nutrients for primary production in the continental shelf area of the East China Sea.

• The Journal of Korean Academy of Orthopedic Manual Physical Therapy
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• v.10 no.1
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• pp.83-101
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• 2004

The propose of the study was to evaluate the efficacy of the partial body weight support during treadmill training on the ambulation in elderly with chronic stroke. Fourteen hemiplegic volunteers participated and were divided into an experimental and control groups. In the experimental group, the body weight support during treadmill training was performed 3 times per week for 6 weeks. In the control group, usual treadmill training was applied. Before and after experiments, temporal-spatial gait parameters were measured. The date of 14 patients who carried out the whole experimental course were statistically analyzed. The results of the study were : 1. In the comparison of gait velocity before and after experiment, the gait velocity was significantly increased in the experimental group and the control group(p<.05). In the comparison of difference of the gait velocity between groups, there was not, significant difference between the experimental group and the control group(p>.05). 2. In comparison of gait cadence before and after experiment, the gait cadence was significantly increased in both groups(p<.05). In the comparison of difference of the gait cadence between groups, there was not significant difference between the experimental group and the control group(p>.05). 3. In the comparison of step length before and after experiment, the step length was significantly increased in the experimental group and the control group(p<.05). In the comparison of difference of the step length between groups, there was not significant difference between the experimental group and the control group(p>.05). 4. In the comparison of single support time asymmetry before and after experiment, the single support time asymmetry was no significant difference between groups(p>.05). In the comparison of difference of the single support time asymmetry between groups, there was not significant difference between the experimental group and the control group(p>.05). 5. In the comparison of step length asymmetry before and. after experiment, the step length asymmetry was not significant difference between the experimental group and the control group(p>.05). In the comparison of difference of the single step length asymmetry between groups, there was not significant difference between the experimental group and the control group(p>.05).

• Journal of the Korean Association of Geographic Information Studies
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• v.7 no.3
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• pp.22-34
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• 2004

An artificial disturbance like fragmentation in watershed forest has impacted a sustainability of watershed ecosystem, therefore this research quantified the landscape structure in the Geumho river watershed using landscape indices and GIS. Landscape indices were calculated from the forest distributed maps for 24 subwatersheds. Three common factors, which explained about 85% of the variation in the original data, were extracted by a factor analysis. The fragmentation gradient in forest landscape, which was calculated from the factor scores, was correlated with proportion of urban land (r=0.827, p<0.01, $R^2$=0.685), elevation (r=-0.637, p<0.01) and slope gradient (r=-0.593, p<0.01). The result of the study presented that the methodology and the values of landscape indices to assess the structural patterns of forest landscape for the Geumho river watershed management. Future research will be directed towards the detection of impacts of landscape patterns and their changes on the integrity of watershed environments.

• Journal of the Korean Geographical Society
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• v.41 no.6 s.117
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• pp.701-713
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• 2006

This study considers the case when a district with a high development possibility is set up as a regulation region. Initial assumption is that there would be regulation resistance in and around the regulation region. Pyeongchang is taken as a study areas, performing necessary analysis of environment regulation and regulation resistance based upon spatial analysis with the use of GIS. The research proceeding steps are as follows. After examining Pyeongchang present environment regulation state, environment regulation map is constructed, and classification of Pyeongchang's relatively development possibility level map is made. Using this regulation map and development possibility level map, relative regulation resistance level is measured. The results that the rates of Pyeongchang environment regulation region in its present state was higher than Namhan river basin's regulation region rates. Also, overlapping a high development possibility level region with an environment regulation region, the analysis results give reasons to expect high level of regulation resistance in Doam-myeon and Jinbu-myeon in small town units. The analysis of Pyeongchang new buildings locations with regard to environment regulation showed intensive distribution in the regulation region with high development possibility level and adjacent areas (about 66% of new buildings are distributed within 500m range from regulation region). Such results show that the local administration and existing environment regulation policy, not capable of offering direct benefit to the population, are having immanent problems in environment sustainable development and connected with it environment harm causation.

• Journal of Bio-Environment Control
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• v.25 no.1
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• pp.49-56
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• 2016

This study was conducted to develop the disinfection system using electric shock for recycled nutrient solution in recycling soilless culture. Stainless steel (SUS 316) was found as the most appropriate electrode material for electrical disinfection system from the view of high electrical conductivity, low electric resistance, and low price. There were no changes in nutritional elements when the electric shock passed through the nutrient solution by stainless steel electrode. The amount of electric current increased with width than thickness of the electrode. The farther the distance between the electrodes was increased the time to reach out the aimed amount of current. The electric shock was applied to Ralstonia solanacearum and Fusarium oxysporum as representative bacteria and also fungi. Any of those pathogens were killed with the percentage of higher than 97% in the condition of 15VDC or 24VDC.

• Journal of Life Science
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• v.28 no.4
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• pp.444-453
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• 2018

Barley (Hordeum vulgare L.), of the Poaceae/Gramineae family, is a common grain in the surrounding area. It has been used in Ancient Egyptian medicine and it has been used worldwide for many years as food and as an ingredient in beer. Barley has been reported to have anti-inflammatory, anti -carcinogenic and anti-diabetic effects. So far, a lot of research has been done on barley but the effects of fermented barley seeds with lactic acid bacteria have not been studied largely. In this study, we investigated the effects of ethanol-extracted barley seeds after their fermentation with lactic acid bacteria. The biological activities of fermented barley seeds with lactic acid bacteria and non-fermented barley seeds were analyzed for total polyphenol content, total flavonoid content, DPPH radical scavenging, superoxide dismutase-like activity and tyrosinase inhibition. These results showed that fermented barley seeds with lactic acid bacteria have more advanced anti-oxidant and whitening properties than non-fermented barley seeds. Hence, we suggest that fermenting barley seeds with lactic acid bacteria can be an impressive material in the food and cosmetic industries.

• Journal of Korea Spatial Information System Society
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• v.4 no.2 s.8
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• pp.55-64
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• 2002

VEfficient generation of road data is one of the most important issues in GIS (Geographic Information System). In this paper, we propose a hybrid approach for automatic generation of road data by combining mobile mapping and image processing techniques. Mobile mapping systems have a form of vehicle equipped with CCD camera, GPS, and INS. They can calculate absolute position of objects that appear in acquired image by photogrammetry, but it is labor-intensive and time-consuming. Automatic road detection methods have been studied also by image processing technology. However, the methods are likely to fail because of obstacles and exceptive conditions in the real world. To overcome the problems, we suggest a hybrid method for automatic road generation, by exploiting both GPS/INS data acquired by mobile mapping system and image processing algorithms. We design an estimator to estimate 3-D coordinates of road line and corresponding location in an image. The estimation process reduces complicated image processing operations that find road line. The missing coordinates of road line due to failure of estimation are obtained by cubic spline interpolation. The interpolation is done piecewise, separated by rapid change such as road intersection. We present experimental results of the suggested estimation and interpolation methods with image sequences acquired by mobile mapping system, and show that the methods are effective in generation of road data.