• Journal of Plant Biology
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• 제35권2호
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• pp.99-106
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• 1992

인삼(Panax ginseng C.A. Meyer) 종자단백질인 vicilin을 ammonium sulfate 침전, gel permeation 및 이온 교환 크로마토그래피로 정제하였다. Vicilin은 분자량 55,000(큰 소단위) 및 44,000(작은 소단위)인 두 종의 소단위를 포함하는 당단백질이다. Vicilin에 대한 항체를 토끼에서 형성시켜 DEAE-Affi-Gel Blue affinity 크로마토그래피로 정제하였다. 이 항체와 금 입자가 결합된 2차 항체를 종자의 배유세포에 반응시켰다. 금 입자는 배유세포내의 단백질체, 전자밀도가 높은 과립 및 골지체의 elaborating 과립에 표지되었다. 이러한 결과는 조면소포체에서 합성되어 골지체로 수송된 vicilin이 골지의 소포내에서 공정과정을 거쳐 전자밀도가 높은 과립이 된 다음 단백질로 수송됨을 나타낸다.

• 미생물학회지
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• 제29권2호
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• pp.92-96
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• 1991

The soluble cytochrome c-551 of photosynthetic bacterium, Rhodopseudomonas gelatinosa ATCC 17013 was purified through a sequene of four step chromatography including CM-cellulose ion-exchange chromatography, DEAE-Sephacel chromatography, Sephacryl s-200 gel permeation chromatography, and HPLC (SP-5PW). The molecular weight of the purified cytochrome c-551 was 14, 600 Da, and this protein shows the absorption peak at 551 nm, 522 nm, and 417 nm as the reduced form, and at 412 nm as the oxidized form. The cytochrome c-551 seems to be a substrate for the terminal oxidase in the electron transport chain.

• Fisheries and Aquatic Sciences
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• 제13권1호
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• pp.84-87
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• 2010

A peptide that inhibits HIV-1 protease was isolated from a hydrolysate of oyster (Crassostrea gigas) proteins digested with thermolysin. The peptide was using membrane filtration, gel permeation chromatography, ion exchange chromatography, and reverse-phase high performance liquid chromatography. Amino acid sequence of the peptide was determined to be Val-Phe-Glu-Leu. Chemically synthesized Val-Phe-Glu-Leu showed an $IC_{50}$ value of 106 ${\mu}M$.

• 한국수산과학회지
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• 제38권2호
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• pp.83-88
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• 2005

Protease inhibitors were purified from the eggs of Alaska pollock (Theragra chalcogramma) using the following purification steps: ammonium sulfate precipitation, ion exchange, gel permeation, and high performance liquid chromatographies (HPLC). The protease inhibitor from the heated eggs of Alaska pollock was not as well purified. In addition, the heated eggs showed lower specific inhibitory activity than the unheated eggs. The purification yields after ammonium sulfate precipitation, ion exchange, and gel permeation chromatographies were 22.7$\%,\;15.3\%$,and $4.4\%$, respectively. There were two kinds of protease inhibitors on the gel permeation chromatography pattern Their molecular weights were estimated to be 66,700 and 16,000 Da, respectively. Both were classified as a cysteine protease inhibitor because of the existence of inhibiting papain, which is one of cysteine proteases.

• Applied Biological Chemistry
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• 제41권4호
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• pp.270-272
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• 1998

Angiotensin converting enzyme (ACE) inhibitor was isolated from beef bone extract hydrolysate. After hydrolysis of beef bone extract with a commercial protease, ACE inhibitory peptide was purified by using ultrafiltration, gel permeation chromatography, and reverse-phase high pressure liquid chromatography. The purified ACE inhibitor was a pentapeptide, Gly-Pro-X-Gly-Pro.

• 한국의류학회지
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• 제22권4호
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• pp.477-481
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• 1998

Colored components in black tea were extracted, freeze-dried, and analysed to investigate the possibility using as a natural dye. Fractionation of the colored components was carried out by gel permeation chromatography. The colored components in black tea were elected into seven fractions. Each fraction was analyzed by UV spectrophotometer. The early fluted fractions 1-4 did not show any absorption peaks in 320-700 nm and showed the increase in absorption as it approaches to short wavelength and are considered as highly polymerized colored substances. Fractions 5-6 showed tar at 350 m and are considered as thearubigins. Fraction 7 showed absorption peaks at 376 nm and 456 nm and is considered as theaflavin. IR spectra of each fraction show: Strong C=0 stretching band at 1650 cm-1 appears in fractions 1-4, but not in fractions 5-7. Strong C=0 stretching band at 1700 cm-L appears in fraction 3-7. C=0 stretching band at 1610 cm-1 appears as a shoulder in fraction 4 and progressively changes into strong peak in fraction 5-7. From these results, it is assumed that colored components in black tea consist of polyphenolic substances having different molecular weight which were formed during tea manufacturing process. The colorants from black tea infusion were applied to silk, wool, cotton and nylon fabrics. Black tea colorants showed high affinity to wool, silk and nylon, but very low affinity to cotton fabrics.

• 폴리머
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• 제39권1호
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• pp.165-168
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• 2015

하이드록시 그룹을 지닌 온도민감형 고분자들이 원자전이라디칼중합법(ATRP)과 클릭반응(click reaction)에 의해 합성되어졌다. 고분자들의 분자량과 분자량 분포도는 gel permeation chromatography(GPC)에 의하여 얻어졌고, 고분자들의 분자량은 잘 제어되었으며 분자량 분포도도 낮게 유지되었다. 클릭반응의 효율은 $^1H$ NMR spectroscopy에 의해 얻어졌으며, 높은 효율을 나타내었다. 고분자 사슬 곁가지의 아민 그룹의 종류와, 치환된 알코올 그룹의 종류에 따라 저임계 용액 온도(LCST)의 제어가 가능했다.

• 한국신재생에너지학회:학술대회논문집
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• 한국신재생에너지학회 2008년도 춘계학술대회 논문집
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• pp.206-209
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• 2008

The modified supercritical water treatment method is adopted for hydrolysis of wood powder, Populus alba$\times$glandulosa. This modified method is containing 0.05% HCl or $HNO_3$ as acid catalyst. The supercritical water treatment(SCW) was performed for 1 min. with $350^{\circ}C$, $380^{\circ}C$, $400^{\circ}C$ and $425^{\circ}C$, respectively, under 230 $\pm$ 10 atm using continuous flow system. When acid was added to powder prepared for SCW treatment, the yields of monomeric sugars were significantly increased. The lignin remained after supercritical treatment was applied to gel permeation chromatography(GPC) for molecular weight distribution analysis. Compared to the lignin produced from SCW treatment without acid catalyst, the average molecular weight of lignin compounds treated with acid was clearly decreased. Particularly, Mn/Mw ratio is decreased. This result shows supercritical water treatment of wood powder can change the molecular weight of lignin to small size. However, it is necessary to be further studied for exactly characterizing the lignin produced from supercritical water treatment.

• 한국균학회지
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• 제40권2호
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• pp.109-113
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• 2012

식용버섯으로부터 항보체 활성물질을 탐색하여 새로운 소재화 연구의 일환으로 상황버섯(Phellinus linteus) 균사체로부터 항보체 활성 물질을 분리, 정제를 하였으며 화학적 특성을 검토하였다. 정제는 상황버섯의 열수추출한 후, 열수추출물(PL-0)를 한외여과하여 분자량별로 5개의 획분을 얻었다. 이 중, 300kDa 이상의 획분(PL-5)을 DEAE-Sepharose FF 이온교환 크로마토그래피, Sepharose CL-4B 겔여과 크로마토그래피를 실시하여 PL-5-IIIa 획분을 얻었다. PL-5-IIIa 획분을 HPLC를 행한 결과 단일 피크로 나타났으며 정제다당 PL-5-IIIa의 분자량은 800,000Da으로 측정되었다. 정제다당 PL-5IIIa는 64.2%의 당과 29.6%의 단백질로 구성되었으며 구성당은 galactose가 43.1%, mannose가 40.6%, fucose가 15.8%로 나타났다.

• 한국식품영양과학회지
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• 제32권7호
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• pp.1120-1125
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• 2003

숙성기간(1, 3 및 3년)이 다른 멸치액젓으로부터 추출한 peptide는 정제과정에서 숙성 1년의 멸치액젓은 3개, 숙성 3년의 멸치액젓은 4개, 숙성 5년의 멸치액젓은 5개의 peak를 각각 나타내었다. 숙성기간이 길수록 멸치액젓의 Peptide는 저분자화 되었으며, gel chromatography상의 후반부에 나타났다. 숙성기간이 다른 멸치액젓으로부터 추출한 peptide들의 생리활성 (항산화, 항암, ACE 저해활성)은 숙성기간이 길수록 증가하였다. 특히 항산화 및 항암활성은 $IC_{50}$/이 각각 34 및 44 $\mu\textrm{g}$/mL인 3년 숙성 peak 3이 제일 높았으나, ACE 저해활성 은 $IC_{50}$/이 32 $\mu\textrm{g}$/mL인 1년 숙성 peak 3이 제일 높았다. 천연 숙성 멸치액젓의 저분자 peptide는 뛰어난 생리활성을 갖고 있음이 밝혀졌으나 구조분석 등의 추가 연구가 필요하다고 본다.