The Korean Journal of Mycology (한국균학회지)

The Korean Society of Mycology (한국균학회)
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Purification and Characterization of Anti-complementary Polysaccharide from Phellinus linteus Mycelia

상황버섯(Phellinus linteus) 균사체로부터 항보체 활성 다당류의 정제 및 특성

  • Seo, Ho-Chan (Department of Brain Education, University of Brain Education)
  • 서호찬 (국제뇌교육종합대학원대학교 뇌교육학과)
  • Received : 2012.05.10
  • Accepted : 2012.06.20
  • Published : 2012.06.30
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Abstract

We have isolated an anti-complementary polysaccharide from the hot water extracts of Phellinus linteus mycelia. Anti-complementary polysaccharide, PL-5-IIIa, was purified by ultrafiltration, gel permeation chromatography using Sepharose CL-4B. GPC (Sepharose CL-4B) and its homogenicity was demonstrated by HPLC. Using gel permeation chromatography with standard dextrans, its molecular weight was determined as about 800,000 dalton. The purified PL-5-IIIa was identified as a protein bound polysaccharide comprising of 29.6% protein and 64.2% carbohydrate which was composed of fucose(15.8%), galactose(43.1%) and mannose(40.6%).

식용버섯으로부터 항보체 활성물질을 탐색하여 새로운 소재화 연구의 일환으로 상황버섯(Phellinus linteus) 균사체로부터 항보체 활성 물질을 분리, 정제를 하였으며 화학적 특성을 검토하였다. 정제는 상황버섯의 열수추출한 후, 열수추출물(PL-0)를 한외여과하여 분자량별로 5개의 획분을 얻었다. 이 중, 300kDa 이상의 획분(PL-5)을 DEAE-Sepharose FF 이온교환 크로마토그래피, Sepharose CL-4B 겔여과 크로마토그래피를 실시하여 PL-5-IIIa 획분을 얻었다. PL-5-IIIa 획분을 HPLC를 행한 결과 단일 피크로 나타났으며 정제다당 PL-5-IIIa의 분자량은 800,000Da으로 측정되었다. 정제다당 PL-5IIIa는 64.2%의 당과 29.6%의 단백질로 구성되었으며 구성당은 galactose가 43.1%, mannose가 40.6%, fucose가 15.8%로 나타났다.

Keywords

References

  1. Chang, S. T. and Miles, P. G. 1989. Overview of biology of fungi in edible mushroom and their cultivation. CRC press: 41-52.
  2. Chihara, G., Hamuro, H., Maeda, Y., Arai, Y. and Fukuoka, K. 1970. Fractionation and purification of the polysaccharides with marked antitumor activity, especially schizophyllan from Schizophyllum commune. Cancer Res 30: 2776-2781.
  3. Chihara, G., Mamuro, J., Maeda, Y., Arai, Y. and Fukuoka, F. 1976. Fractionation and purification of the polysaccharide with marked anti-tumor activity especially lentinan from Lentinus edodes. Cancer Res 30(11): 2776-2780.
  4. Egwang, T. G. and Befus, A. D. 1984. The role of complement in the induction and regulation of immune reponses. Immunology 15: 207-211.
  5. Gao, Q. P., Kiyohara, H., Cyong, J. C. and Yamada, H. 1989. Chemical properties and anti-complementary activities of polysaccharide fractions from roots and leaves of Panax ginseng. Planta Med 55: 9-17. https://doi.org/10.1055/s-2006-961765
  6. Immunochemistry, Thomas Publisher (2nd ed.), pp. 459-461.
  7. Lee, H. S., Kweon, M. H., Lim, W. J., Sung, H. C. and Yang, H. C. 1997. An anticoagulant polysaccharide isolated from the alkali extracts of Coriolus versicolor. Korean J Food Sci Technol 29: 369-375.
  8. Lin F. M., Pomeranz, Y. 1968. Effect of borate on colorimetric determinations of carbohydrates by the phenol-sulfuric acid. Anal Biochem 24(1):128-131. https://doi.org/10.1016/0003-2697(68)90067-5
  9. Lowry, O. H., Rosebrough, N. J. and Rindall, R. J. 1951. Protein measurement with the folin phenol reagent. J Biol Chem 193: 256-260.
  10. Mayer, M. M. 1961. On the destruction of erythrocytes and other cells by antibody and complement. Cancer Res 21: 1262-1269.
  11. Ohno, N., Iino, K., Suzuki, I., Oikawa, S., Sato, K., Miyazaki, T. and Yadomae, T. 1985. Neutral and acidic antitumor polysaccharides extracted from cultured fruit bodies of Griofora frondosa. Chem Pharm Bull 33(3): 1181-1192. https://doi.org/10.1248/cpb.33.1181
  12. Ohno, N., Suzuki, I., Oikawa, S., Sato, K., Miyazaki, T. and Yadomae, T. 1984. Antitumor activity and structure characterization of glucan, extracted from cultured fruit bodies of Grifola frondosa. Chem Pharm Bull 32: 1142-1151. https://doi.org/10.1248/cpb.32.1142
  13. Roit, I., Brostoff, I., Male, D. 1993. Immunology, Mosby.
  14. Sasaki, T. and Takasuka, N. 1976. Further study of the structure of lintinan an anti-tumor polysaccharide from Griofola frondosa. Carbohydr Res 47: 99-109. https://doi.org/10.1016/S0008-6215(00)83552-1
  15. Shimizu, N., Gonda, R., Neiko, K., Kanari, H. and Tomoda, M. 1989. The major pectic arabinogalactan having activity on the reticuloendothelial system grom the roots and rhizomes of Saposhnikovia divaricata. Chem Pharm Bull 37(5): 1329-1333. https://doi.org/10.1248/cpb.37.1329
  16. Song, K. S., Cho, S. M., Lee, J. H., Kim, H. M., Han, S. B., Ko, K. S. and Yoo, I. D. 1995. B-Lymphocyte stimulating polysaccharide from mushroom Phellinus linteus. Chem Pharm Bull 43(12): 2105- 2114. https://doi.org/10.1248/cpb.43.2105
  17. Suzuki, I., Hashimoto, K., Oikawa, S. and Sato, K. 1980. Antitumor and immunomodulating activities of a ‚-glucan obtained from liquid cultured Coriolus versicolor. Chem Pham Bull 37: 410-420.
  18. Yamada, H. 1984. Structure and antitumor activity of alkali-soluble polysaccharide from Cordyceps ophioglossoides. Carbohydrate Research 125: 107-112. https://doi.org/10.1016/0008-6215(84)85146-0
  19. Yamada, H., Ra, K. S., Kiyohara, H. Cyong, J. C. and Otsuka, Y. 1988. Characterization of anti-complementary neutral polysaccharide from the root of Bupleurum falcatum. Phytochem 29: 3163-3170.
  20. Yoshioka, P., Ikekawa, T., Noda, M. and Fukuoka, F. 1972. Studies on antitumor activity of some fractions from basidiomycetes. An antitumor acidic polysaccharide fraction from P. ostreatus. Chem Phar Bull 20: 1233-1242.