• 한국식품영양과학회지
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• 제33권4호
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• pp.659-667
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• 2004

본 실험에서는 흰쥐에서 실험적으로 급성위염, 위궤양 및 십이지장궤양을 유발하고 질경이 에칠아세테이트분획을 투여하여 항위염 및 항위궤양작용에 미치는 영향을 검토하였던 바 다음과 같은 결과를 얻었다. 1. 질경이 에탄올추출물을 n-hexane, chloroform, ethylactae 및 n-butanol로 계통분획하고 농축하여 in vitro에서 항산화력을 측정한 결과 ethyl-acetate분획이 가장 우수하였다. 2. 질경이 에칠아세테이트분획 (PEL 및 PEH)은 HCl-ethanol에 의한 위점막 손상에서 대조군(CON)에 비하여 각 용량별로 55.7% 및 62.1%의 유의적인 항위염작용을 나타냈으며, 특히 고용량투여군인 PEH의 경우 양성 대조약물투여군(60.5%)보다 우수한 효과를 나타냈다. 3. Indomethacin에 의해 유발된 위궤양에서, PEL 및 PEH는 양성 대조약물군인 CMT의 위궤양 억제율(61.6%)에는 다소 미치지 못하였으나 CON에 비하여 유의성 있는 억제 효과를 나타냈다. 4. Shay위궤양에서, 질경이 에칠아세테이트분획 200mg/kg을 십이지장에서 위내로 투여하였을 때 궤양억제율이 37.9%로 CON에 비하여 Shay위궤양을 억제하였으나 유의성있는 효과는 아니었으며 용량을 증량하여 투여한 PEH(400 mg/kg)는 48.9%로 양성 대조약물인 omeprazol를 투여한 OMP의 저해효과(55.6 %)에 근접하였다. 5. 질경이의 에틸아세테이트분획을 용량별로 투여했을 때 위액의 pH가 대조군에 비해 약간의 감소되었으나 정상 범위 내로 유의성은 인정되지 않았고 양성 대조약물군인 OMP는 유의적으로 위액의 pH를 유의성있게 감소시켰다. 위액량 및 총 위산분비량의 경우, 대조군에 비하여 PEL 및 PEH가 각각 33.8%와 38.7%의 감소효과를 나타냈으나 유의성은 없었으며 양성 대조약물인 omeprazol 만큼 뚜렷한 효과는 볼 수 없었다. 6. Pepsin활성에서, 질경이 에틸아세테이트분획과 양성 대조약물투여로 pepsin 활성이 대조군에 비해 각각 약 17%, 22% 및 24%의 활성저해 효과를 나타냈으나 전군에서 유의성 있는 변화는 아니었다. 7 구속ㆍ수침스트레스에 의해 유발된 궤양의 경우, PEL 및 PEH에서도 출혈을 동반한 궤양이 관찰되었으나 CON에 비하여 궤양의 깊이가 다소 얕았지만 유의적인 효과는 아니었다. 양성 약물대조군의 경우 궤양의 형성이 CON과 PEL및 PEH에 비하여 궤양형성의 부분이 다소 적었으나 점상 출혈이 존재하였다. 8. Cysteamine에 의해 유발된 십이지장궤양에서, CMT가 52.2%의 유의한 궤양 억제율을 나타냈고 PEH의 경우 유의성은 없었으나 약 43%의 궤양억제율을 나타냈다.

• Journal of Pharmaceutical Investigation
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• 제17권4호
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• pp.189-195
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• 1987

The effects of cimetidine or ranitidine pretreatment on the intestinal absorption, plasma and urine level of caffeine, gastric acidity of mouse, and sleeping time by hexobarbital sodium were investigated pharmacokinetically. Cimetidine and ranitidine pretreatments were found to increase both the rate and extent of absorption of caffeine in rats. Cimetidine pretreatment increased blood level of caffeine and decreased urine level, while ranitidine pretreatment had no effect on urine level of caffeine. Ranitidine pretreatment inhibited gastric secretion due to caffeine more than cimetidine pretreatment. Cimetidine pretreatment increased the action of caffeine and showed shorter sleeping time by hexobarbital sodium, comparing with ranitidine pretreatment.

• 대한한방내과학회지
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• 제15권2호
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• pp.197-208
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• 1994

In order to investigate the efficacy of Siljangsan by using the experimental animals, the action of gastric juice secretion, antiulcer. transport rates in the intestine and anti-cathartic action were studied. The following results were obtained. 1. Preventive effects on ulcer induced by pylorus-ligated and Ethanol-HCI were recognized 2. Suppression effects on gastric juice secretinon, free of acidity and total acidity were recognized, but pepsin output was not recognized. 3. For the transport ability of intestine of mice, the ability of small intestine was activated and large intestine was suppressed in the group administrated 4. Anti-cathatic action of mice induced by castor oil was recognized. According to above results, effect based on oriental medical reference was consistent with the actual experimental effect.

• 대한한방내과학회지
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• 제15권2호
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• pp.37-47
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• 1994

To investigate the effect of ILGUAN-JEON on liver and gastrointestinal track. this experiment were performed and obtained results wewe as follows. 1. ILGUAN-JEON depressed the isolated intestine movement and showed anti- acethylcholine and barium chloride effect in mice. 2. ILGUAN-JEON showed anti- acethylcholine and barium chloride effect on isolated stomach in rats. 3. ILGUAN-JEON showed anti-ulcer effect in rats induced by indomethacin and pyrous cracking. 4. ILGUAN-JEON depressed the gastric lesion in rats induced by HCI-ethanol. 5. ILGUAN-JEON depressed the gastric juice and pepsin secretion, free acid and total acid level in Shay rats. 6. ILGUAN-JEON depressed the GOT and GPT level in rats induced by $CCl_4$ and d-galactosamine. From above results, think that ILGUAN-JEON can be applied to recover the liver and gastrointestinal function.

• 대한한방소아과학회지
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• 제1권1호
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• pp.23-37
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• 1986

Although the Insamyangwee-Tang has been widely used in clinical purposes in the oriental medicine and its clinical efficiency is documented for the cases of gastritis, gastric ulcer and enteritis but the experimental study on these has not been undertaken. So,To investigate the clinical efficiency, of Insamyangwee-Tang and validate its oriental medical theory, these experiments were undertaken, by being compared with animal experiment. ?The following results were obtained. ?1. By effect of Insamyangwee-Tang on isolated ileum from mice, rats, rabbits and Guinea pigs, the motility of ileums was inhibited remarkably. ?2. By effect of Insamyangwee-Tang on motility of the small intestine in rabbits, the motility was inhibited remarkably too. ?3. Insamyangwee-Tang inhibited charcoal transport functions in the small intestine of mice. ?4. By effect of Insamyangwee-Tang on isolated duodenum from rats, the motility of duodenum was inhibited remarkably.?5. Gastric juice and pepsin secretion was decreased, anti-ulceration effect was recognized.

• 고려인삼학회:학술대회논문집
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• 고려인삼학회 2002년도 학술대회지
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• pp.392-400
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• 2002

We have previously found that the saponins but not other components in the ginseng reduce the secretion of catecholamines (CAs) from bovine adrenal chromaffin cells, a model of sympathetic nerves, evoked by acetylcholine (ACh) due to the blockade of $Na^+$ influx through nicotinic ACh receptor-operated cation channels, and it has been concluded that the inhibitory effect may be associated with the anti-stress action of ginseng. However, the saponins, which showed the great reduction of the CA secretion, were mainly the protopanaxiatriols. The protopanaxadiol and oleanolic acid saponins had a little or little such effect. Recent studies demonstrated that the oligosaccharides connected to the hydroxyl groups of the aglycones of the saponins are in turn hydrolyzed by gastric acid and enzymes in the intestinal bacteria when the ginseng is orally administrated. In this study, the effects of their major 6 kinds of metabolites on the secretion of CAs were investigated. All metabolites (M1, 2, 3 and 5 derived from the protopanaxadiols, and M4 and 11 from the protopanaxiatriols) reduced the ACh-evoked secretion from the cells. In the metabolites, the M4 inhibition was the most potent ($IC_{50}({\mu}M):M4(9)$ < M2 (18) < M3 (19) < M1l (22) < M5 (36) < MI (38)). Although M4 also reduced the CA secretion induced by high $K^+$, a stimulation activating voltage-sensitive $Ca^{2+}$ channels, the inhibitory effect was much less than that on the ACh-evoked secretion. M4 inhibited the ACh-induced $Na^+$ influx into the cells in a concentration-dependent manner similar to that of the inhibition of the ACh-evoked secretion. When the cells were washed by the incubation buffer after the preincubation of the cells with M4 and then incubated without M4 in the presence of ACh, the M4 inhibition was not completely abolished. On the other hand, its inhibition was maintained even by increasing the external ACh concentration. These results indicate that the saponins are metabolized to the more active substances in the digestive tract and the metabolites attenuate the secretion of CAs from bovine adrenal chromaffin cells stimulated by ACh due to the noncompetitive blockade of the ACh-induced $Na^+$ influx into the cells. These findings may further explain the anti-stress action of ginseng.

• Advances in Traditional Medicine
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• 제10권1호
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• pp.13-20
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• 2010

The leaves of Madhuca (M.) indica J.f.Gmel. (Sapotaceae) have been used traditionally in folk medicine due to its astringent properties and are effective in treatment of eczema and snake bites. Methanolic extract of M. indica is rich in tannins and has been proven experimentally to possess antibacterial activity. The present study was undertaken to evaluate the anti-ulcer and antioxidant activity of M. indica in rats. The methanolic extract of leaves of M. indica was tested at various doses (75, 150 and 300 mg/kg, p.o.) for its effect on gastric secretion and gastric ulcers in pylorus-ligation and on ethanol- induced gastric mucosal injury in rats. The significant reduction in ulcer index in both the models along with an increase in the pH of the gastric fluid and mucin content of stomach, and the acid secretory parameters such as total acidity and volume of gastric fluid were also significantly reduced along with reduction in the pepsin activity in pylorusligated rats proved the anti-ulcer activity of M. indica. The increase in the levels of superoxide dismutase, catalase and reduced glutathione and decrease in lipid peroxidation in both the models proved the antioxidant activity of M. indica. Thus it can be concluded that M. indica possesses anti-ulcer activity, which can be attributed to its antioxidant mechanism of action.

• Archives of Pharmacal Research
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• 제29권6호
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• pp.484-489
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• 2006

This Study evaluated the inhibitory action of $luteolin-7-O-{\beta}-D-glucuronopyranoside$, luteolin which was isolated from Salix gilgiana leaves, and omeprazole on reflux esophagitis and gastritis in rats. Reflux esophagitis and gastritis were induced surgically and by the administration of indomethacin, respectively. The intraduodenal administration of $luteolin-7-O-{\beta}-D-glucuronopyranoside$ decreased the ulcer index, injury area, gastric volume and acid output, and increased the gastric pH compared with luteolin. $Luteolin-7-O-{\beta}-D-glucuronopyranoside$ significantly decreased the size of the gastric lesions that had been induced by exposing the gastric mucosa to indomethacin. The malondialdehyde content, which is the end product of lipid peroxidation, was increased significantly after inducing of reflux esophagitis. The malondialdehyde content was decreased by $Luteolin-7-O-{\beta}-D-glucuronopyranoside$ but not luteolin or omeprazole. $Luteolin-7-O-{\beta}-D-glucuronopyranoside$ has a more potent antioxidative effect than luteolin. $Luteolin-7-O-{\beta}-D-glucuronopyranoside$ is a promising drug for the treatment of reflux esophagitis and gastritis.

• 한국식품영양학회지
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• 제34권5호
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• pp.468-476
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• 2021

In recent years, there has been an increase in the morbidity of gastritis in Korea due to lifestyle factors mostly changes in eating habits and stress. Gastritis is more likely to progress to gastric cancer, and therefore it is important to prevent and manage gastritis through lifestyle adjustment and treatment at an early stage. In this study, cabbage, which was found to be effective in gastritis, was mixed and fermented with other crucifer plants such as kale and broccoli to evaluate the overall efficacy of fermented brassica puree on alcoholic acute gastritis. Based on our results, fermented brassica puree alleviated gastric injury induced by 150 mM HCl/60% ethanol. In addition, it was confirmed that PGE₂, a gastric mucosal protective factor, was increased, and other positive effects such as an increase of MUC1 and regulation of PKC were observed. The results of this study suggest that fermented brassica puree can relieve acute alcoholic gastritis by regulating PGE and the expression of MUC1, a gene related to mucus secretion, and activating PKC, which is related to mucosal cell activity.

• Experimental and Molecular Medicine
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• 제50권12호
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• pp.1.1-1.14
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• 2018

DNA methylation is a regulatory mechanism in epigenetics that is frequently altered during human carcinogenesis. To detect critical methylation events associated with gastric cancer (GC), we compared three DNA methylomes from gastric mucosa (GM), intestinal metaplasia (IM), and gastric tumor (GT) cells that were microscopically dissected from an intestinal-type early gastric cancer (EGC) using methylated DNA binding domain sequencing (MBD-seq) and reduced representation bisulfite sequencing (RRBS) analysis. In this study, we focused on differentially methylated promoters (DMPs) that could be directly associated with gene expression. We detected 2,761 and 677 DMPs between the GT and GM by MBD-seq and RRBS, respectively, and for a total of 3,035 DMPs. Then, 514 (17%) of all DMPs were detected in the IM genome, which is a precancer of GC, supporting that some DMPs might represent an early event in gastric carcinogenesis. A pathway analysis of all DMPs demonstrated that 59 G protein-coupled receptor (GPCR) genes linked to the hypermethylated DMPs were significantly enriched in a neuroactive ligand-receptor interaction pathway. Furthermore, among the 59 GPCRs, six GI hormone receptor genes (NPY1R, PPYR1, PTGDR, PTGER2, PTGER3, and SSTR2) that play an inhibitory role in the secretion of gastrin or gastric acid were selected and validated as potential biomarkers for the diagnosis or prognosis of GC patients in two cohorts. These data suggest that the loss of function of gastrointestinal (GI) hormone receptors by promoter methylation may lead to gastric carcinogenesis because gastrin and gastric acid have been known to play a role in cell differentiation and carcinogenesis in the GI tract.