• Investigative Magnetic Resonance Imaging
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• 제13권2호
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• pp.213-217
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• 2009

간에 생기는 단발성 괴사성 결절은 매우 드문 양성 질환으로 대개 아무 증상 없이 우연히 발견되고, 간에서 생길 수 있는 여러 가지 단발성 병변과의 감별을 필요로 하며, 특히 악성 종양의 병기 결정에 있어서 간내 전이암과 구별하는 것은 매우 중요하다고 알려져 있다. 영상의학적으로 간의 단발성 괴사성 결절은 조영증강이 얼거나 미약한 주변부 조영증강을 보이는 경계가 좋은 간내 결절로 보고되고 있다. 하지만 단발성 괴사성 결절이 영상에서 과녁 모양으로 보이는 경우는 지금까지 보고된 바 없으며, 특히 고자장의 자기 공명 영상과 양성자 방출 단층 촬영 소견이 보고된 경우도 전무하다. 이에 저자들은 조기 위암 환자에서 과녁 모양으로 보여 전이암과 감별이 어려웠던 간내 단발성 결절을 경험하여 이의 다양한 영상 소견을 보고하고자 하며, 병리학적 소견과 이에 대한 문헌 고찰을 통해 원인을 알아 보고자 하였다.

• 대한미생물학회지
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• 제34권6호
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• pp.543-554
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• 1999

The purpose of this study was to evaluate the existence of amoxicillin, clarithromycin, and metronidazole resistance Helicobacter pylori and to determine the in-vitro MIC of two and three kinds of antibiotic concominant administration in the isolates. The antimicrobial agents tested against 169 H. pylori included metronidazole, amoxicillin, ciprofloxacin, clarithromycin, omeprazole, josamycin, erythromycin, and tetracycline. MIC of each antimicrobial agents was determined by broth microdilution method. The 169 strains of H. pylori were isolated from biopsy specimens of patients with gastric cancer. $MIC_{50}$ of clarithromycin, amoxicillin, metronidazole, omeprazole, erythromycin, josamycin, tetracycline, and ciprofloxacin was 2.0, 1.0, 4.0, 8.0, 0.5, 0.5, and $0.5\;{\mu}g/ml$, respectively. $MIC_{90}$ of clarithromycin, amoxicillin, metronidazole, omeprazole, erythromycin, josamycin, tetracycline, and ciprofloxacin was 64.0, 64.0, 32.0, 16.0, 8.0, 2.0, and $1.0\;{\mu}g/ml$, respectively. H. pylori isolates were detected in the following resistaince rates: 34.3% to clarithromycin, 31.9% to metronidazole, 20.7% to amoxicillin, 12.4% to erythromycin, and 10.1% to josamycin. The prevalence of the antibiotic resistant strains of H. pylori were detected 18.1% for two kind of antibiotics and 9.6% for three kind of antibiotics, and 3.9% for four kind of antibiotics. The $MIC_{90}$ of clarithromycin-, metronidazole-, and amoxicillin-resistant H. pylori was decreased under the $1\;{\mu}g/ml$ by the two or three kind of antibiotic concomitant administration in-vitro. These results suggest that two or three antibiotics concomitant administration could be more effective for the treatment of clarithromycin-, amoxicillin-, metronidazole-, and josamycin-resistant H. pylori strains.

• Journal of Microbiology and Biotechnology
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• 제10권6호
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• pp.865-872
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• 2000

Helocobacter phylori is the major cause of gastritis, peptic ulcer, and a principal risk factor for gastric cancer. As the firs step towards a vaccine against H. pylori infection, Hy.pylori urease was expressed and purified as a recombinant apoenzyme (rUrease) in E. coli. In order to develop an effective immunization protocol using rUrease, the host immune responses were evaluated after the oral immunization of mice with rUrease preparations plus cholera toxin relative to various conditions, such as the physical nature of the antigen, the frequency of the booster immunization, the dose of the antigen, and the route of administration. The protective efficacy was assessed using a quantitative culture following an H. pylori SS1 challenge. It was demonstrated that rUrease, due to its particulated nature, was more superior than the UreB subunit as a vaccine antigen. The oral immunization of rUrease elicited significant systemic and secretory antibody responses, and activated predominantly Th2-type cellular responses. The bacterial colonization was significantly reduced (~100-fold) in those mice immunized with three or four weekly oran doses of rUrease plus cholera toxin (p<0.05), when compared to the non-immunized/challenged controls. The protection correlated well with the elicited secretory IgA level against rUrease, and these secretory antibody responses were highly dependent on the frequency of the booster immunization, yet unaffected by the dose of the antigen (25-200$\mu\textrm{g}$). These results demonstrate the remarkable potential of rUrease as a vaccine antigen, thereby strengthening the possibility of developing an H. pylori vaccine for humans.

• 대한핵의학회지
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• 제22권2호
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• pp.199-203
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• 1988

The authors observed functional change of the CBD and the afferent loop with the transit time of $^{99m}Tc-DISIDA$ scintigram in patients treated by the surgical operation of gastric ulcers and cancer at Busan National University Hospital from June, 1987 to April, 1988. So authors estimated the time between the peak activity of the CBD and the afferent loop (TCA), the half transit time of the CBD activity (t 1/2 CBD) and the half transit time of the afferent loop activity (t 1/2 A-loop) in 9 patients undergone truncal vagotomy, who have no specific symptoms at 2 to 6 months after the operation, 5 patients undergone the operation without truncal vagotomy, and 7 persons as a control group. The results were as follows: I) In control group, the mean TCA, t 1/2 CBD and t 1/2 duodenum were $8.6{\pm}9.0$ min, $38.7{\pm}17.3$ min. and $28.6{\pm}10.9$ min, respectively. 2) In the operation group without truncal vagotomy, the mean TCA, t 1/2 CBD and t 1/2 A-loop were $10.0{\pm}7.1$ min. $48.2{\pm}22.3$ min. and $39.4{\pm}26.7$ min. respectively. 3) In the operation group with truncal vagotomy, the mean TCA, t 1/2 CBD and t 1/2 A-loop were $15.0{\pm}8.7$ min., $36.1{\pm}16.5$ min. and $42.6{\pm}24.4$ min, respectively. From the above results, we concluded that the transit time of the CBD and the afferent loop is not affected by truncal vagotomy.

• Archives of Plastic Surgery
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• 제35권5호
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• pp.626-629
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• 2008

Purpose: We investigate the possibility of vessel preservation with glycerol and evaluate the potential of preserved gastroepiploic artery as a tool for the microsurgical practice. Methods: In 5 early gastric cancer patients, IRB(No. C-0603-040-170), we gained gastroepiploic artery specimens(5 segments, about 10 cm) after gastrectomy. Each segment was rinsed in a normal saline and subsequently placed in a bottle, containing 50 mL anhydrous glycerol (87%). The bottles were refrigerated at $4^{\circ}C$, the longest preservation time being 5 months. Results: At first glance, no fragmentation was detected and the surface of vessel seemed mild sclerotic. In histological examination, vascular structures remained intact though preservation with glycerol led to a mild atrophy of the smooth muscle in the tunica media. Especially, we found out the elastic fibers and endothelial lining of the intima were preserved until 5 months. Adequate status for using microsurgical practice was also maintained and leakage test was performed successfully with gentian violet ink. Conclusion: Based on the results obtained in this study, the gastroepiploic artery preservation with glycerol as a training model for microvascular anastomosis is a technically very simple procedure and useful for the novice microsurgeon.

• Food Science and Biotechnology
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• 제16권5호
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• pp.843-847
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• 2007

Strain NK34 was characterized for probiotic use. Strain NK34 was named Lactococcus lactis NK34 based on API 50 CHL kit results and 16S rDNA sequencing. L. lactis NK34 was highly resistant to artificial gastric juice (pH 2.5) and artificial bile acid. Based on results from the API ZYM kit, 4 enzymes were produced. L. lactis NK34 was resistant to all antibiotics tested except for $10\;{\mu}g/mL$ roxithromycin and $10\;{\mu}g/mL$ erythromycin. The cholesterol-lowering effect of L. lactis NK34 was about 46.9%. Concentrations of interleukin $(IL)-1{\alpha}$ in the $20{\times}$ concentrated supernatant of L. lactis NK34 was about 361 pg/mL. L. lactis NK34 was also found to inhibit the growth of colon cancer cells due to MNNG-induced DNA damage. These results demonstrate the potential of L. lactis NK34 as a health-promoting probiotic.

• 한국자원식물학회:학술대회논문집
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• 한국자원식물학회 2018년도 추계학술대회
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• pp.127-127
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• 2018

Probiotics are microorganisms that have beneficial effects on the health of the host. The health promoting effect by probiotics influences suppressing harmful bacteria, prevention of constipation, blood cholesterol reduction and regulation of blood pressure. Prebiotics are used to promote the growth or activity of microorganisms. Synbiotics, which are a mixture of probiotics and prebiotics, synergize in the intestines by complementing each other. Synbiotics not only improves the viability of the probiotics while passing through the gastrointestinal tract, maintain intestinal homeostasis, but also regulate balance of harmful and useful bacterial growth. Glycine max Merr (GMM) has been widely used in Asian countries to treat cancer, obesity, oxidative stress and imbalanced immune diseases. In addition, it has been reported that dietary fiber-rich grains promote bowel movements and prevent constipation. In this study, we investigated the viability of LactobacillIus buchneri (L.buchneri) strains, known as lactic acid bacteria under conditions of gastric fluid and intestinal fluid to determine the suitability of L.buchneri as probiotics. The adhesion ability of L.buchneri to caco-2 cells was also confirmed. The present studies showed that GMM extract promoted the growth and activity of L.buchneri strains as prebiotics. Also, this results suggested that the mixture of L.buchneri and GMM extract can helps maintain intestinal health and healthy body as synbiotics and health functional food material.

• Journal of Oral Medicine and Pain
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• 제33권1호
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• pp.9-14
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• 2008

Helicobacter pylori(H. pylori)는 위 십이지장 궤양 및 위암의 발생과 관련되어 있으며, 감염은 대부분 유년기에 발생되어 나이가 먹어감에 따라 증가한다. 감염경로에 있어서 구강은 매우 중요한 역할을 하기 때문에 소아의 타액에서 H. pylori의 발현율을 조사하여 감염경로를 규명하기 위해 위장관과 관련된 증상 및 전신질환이 없는 10세 이하 20명과 10대 20명의 실험군과 20세 이상 71명의 대조군을 대상으로 연구하였다. 10세 미만에서 5명(25%), 10대에서 6명(30%) 그리고 20세 이상 17명(23.9%)에서 H. pylori가 타액에서 검출되었다. 실험군과 대조군간의 검출율 비교에 있어서 차이는 없었다(P>0.05). 이상의 결과를 종합해 볼 때 건강한 한국인 소아의 타액 내에서 H. pylori는 비교적 흔히 존재하며, 구강이 H. pylori 감염에 근원이 될 수 있음을 추론할 수 있다.

• 환경영향평가
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• 제5권1호
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• pp.47-60
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• 1996

지하수를 음용수로 공급시 질산오염문제는 한국을 포함한 세계의 여러나라에서 보고되어 왔다. 질산($NO_3$)은 청색증 유발물짙로서 뿐만 아니라 발암물질일 가능성도 동물실험을 통해 제기되고 있는 실정이다. 질산에 오염된 지하수로 인한 인체의 위해성을 감소시키기 위하여 여러 가지의 질산 위해성 관리 대책들은 개발될 수 있다. 이러한 여러가지의 대책들을 비교 평가할 때 기준이 되는 항목으로는 (1) 허용할 수 있는 인체의 위해도(Risk Level), (2) 비용, 그리고 (3) 적용하는 방법의 기술적 타당성을 들 수 있다. 그러나 불충분한 자료와 인간지식의 한계 때문에 각 기준항목을 대표하는 값의 대부분은 불확실성을 내포하게 된다. 본 논문에서는 의사결정권자들이 불확실성을 고려하면서 여러가지 대책들 중에서 최적의 대책을 선정하는데 이용하기 위한 다기준의사결정기법은 보여진다. 그리고 각 기준값의 불확실성을 표현하고 다기준의사결정기법과 불확실성을 결합시키기 위하여 퍼지집합이론(Fuzzy Set Theory)은 응용되어 진다.

• Molecular & Cellular Toxicology
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• 제3권3호
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• pp.165-171
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• 2007

Mitomycin C (MMC), an antitumor antibiotic isolated from Streptomyces caespitosus, is used in chemotherapy of gastric, bladder and colorectal cancer. MMC is activated in vivo to alkylate and crosslink DNA, via G-G interstrand bonds, thereby inhibiting DNA synthesis and transcription. This study investigates gene expression changes in response to MMC treatment in order to elucidate the mechanisms of MMC-induced toxicity. MMC was admistered with single dose (0.32 and 1.6 ${\mu}M$) to TK6 cells. Applied Biosystem's DNA chips were used for identifying the gene expression profile by MMC-induced toxicity. We identified up- or down-regulated 90 genes including cyclin M2, cyclin-dependent kinase inhibitor 1A (p21, cip1), programmed cell death 1, tumor necrosis factor (ligand) superfamily, member 9, et al. The regulated genes by MMC associated with the biological pathways apoptosis signaling pathway. Further characterization of these candidate markers related to the toxicity will be useful to understand the detailed mechanism of action of MMC.