The Journal of the Korean Society for Microbiology (대한미생물학회지)

The Korea Society for Microbiology (대한미생물학회)
권호 표지

Antibiotic Susceptibility of Helicobacter pylori and the Combination Effect of Antibiotics on the Antibiotic-Resistant H. pylori Strains

위암환자에서 분리된 Helicobacter pylori의 항생물질에 대한 감수성과 저항성 균에 대한 항생제 병합요법의 효과

  • Song, Gap-Young (Department of Microbiology, Kosin University College of Medicine) ;
  • Chang, Myung-Woong (Department of Microbiology, Kosin University College of Medicine)
  • 송갑영 (고신대학교 의과대학 미생물학교실) ;
  • 장명웅 (고신대학교 의과대학 미생물학교실)
  • Published : 1999.12.31
Download PDF
⟨ Previous Next ⟩

Abstract

The purpose of this study was to evaluate the existence of amoxicillin, clarithromycin, and metronidazole resistance Helicobacter pylori and to determine the in-vitro MIC of two and three kinds of antibiotic concominant administration in the isolates. The antimicrobial agents tested against 169 H. pylori included metronidazole, amoxicillin, ciprofloxacin, clarithromycin, omeprazole, josamycin, erythromycin, and tetracycline. MIC of each antimicrobial agents was determined by broth microdilution method. The 169 strains of H. pylori were isolated from biopsy specimens of patients with gastric cancer. $MIC_{50}$ of clarithromycin, amoxicillin, metronidazole, omeprazole, erythromycin, josamycin, tetracycline, and ciprofloxacin was 2.0, 1.0, 4.0, 8.0, 0.5, 0.5, and $0.5\;{\mu}g/ml$, respectively. $MIC_{90}$ of clarithromycin, amoxicillin, metronidazole, omeprazole, erythromycin, josamycin, tetracycline, and ciprofloxacin was 64.0, 64.0, 32.0, 16.0, 8.0, 2.0, and $1.0\;{\mu}g/ml$, respectively. H. pylori isolates were detected in the following resistaince rates: 34.3% to clarithromycin, 31.9% to metronidazole, 20.7% to amoxicillin, 12.4% to erythromycin, and 10.1% to josamycin. The prevalence of the antibiotic resistant strains of H. pylori were detected 18.1% for two kind of antibiotics and 9.6% for three kind of antibiotics, and 3.9% for four kind of antibiotics. The $MIC_{90}$ of clarithromycin-, metronidazole-, and amoxicillin-resistant H. pylori was decreased under the $1\;{\mu}g/ml$ by the two or three kind of antibiotic concomitant administration in-vitro. These results suggest that two or three antibiotics concomitant administration could be more effective for the treatment of clarithromycin-, amoxicillin-, metronidazole-, and josamycin-resistant H. pylori strains.

Keywords

References

  1. 대한미생물학회지 v.23 내시경 생검체에서 분리한 Helicobacter pylori의 생물학적 특성 이광호;조명제;김종배;최상경;김영채
  2. 대한의사협회지 v.40 Helicobacer pylori감염의 세균학적 특성 이광호
  3. 대한미생물학회지 v.32 Helicobacter pylori의 vacuolating toxin생성에 미치는 영향 장명웅;송갑영;차형수
  4. 항생제 내성 소식 v.6 no.2 Helicobacter pylori의 metronidazole 감수성 시험 정윤섭
  5. Gut v.35 High prevalence of Helicobacter pylori metronidazole resistance in migrants to east London: relation resistance in migrants to east London: relation with previous nitronidazole exposure and gastroduodenal disease Banatvala N;Davies GR;Abdi Y (et al.)
  6. Gut v.38 Prevalence of metronidazole resistant Helicobacter pylori strains among Chinese peptic ulcer disease patients and normal controls in Hong Kong Ching CK;Leung KP;Yung RWH(et al.)
  7. J Clin Microbiol v.31 Metronidazole susceptibility testing for Helicobacter pylori: Comparison of disk, and agar dilution methods and their clincial relevance DcCross AJ;Marshall BJ;McCallum EW(et al.)
  8. Antimicrob Agents Chemother v.43 Sensitivity of amoxicillin-resustant Helicobacter pylori to other penicillins Dore MP;Graham DY;Sepulveda AR;Realdi G;Osato MS
  9. Aliment Pharmacol Ther v.12 Amoxycillin resistance is one reason for failure of amoxycillin-omeprazole treatment of H. pylori infection Dore MP;Piana A;Carta M;Atzei A;Are BM (et al)
  10. J Antimicrob Chemother v.43 Amoxycillin tolerance in Helicobacter pylori Dore MP;Osato MS;Realdi G;Mura I (et al.)
  11. Clin Microbiol Rev v.10 Helicobacter pylori Dunn BE;Cohen H;Blaser MJ
  12. J Antimicrob Chemother v.31 Nitronidazole drugsaction and resistance mechanisms. 1. Mechanisms of resistance Edwards DI
  13. Gut v.39 no.SUP. 3 Does Helicobacter pylori infection have a role in primay Sjoengres's syndrome Erdem N;Ersoez G;Avdin A (et al)
  14. Gastroenterol v.112 Antimicrobial susceptibility of H. pylori in Canada to three key antibiotics: metronidazole, clarithromycin, and amoxycillin Fedorak R;Arachambault A;Flamm R;Osaton M;Stamler D
  15. NCCLS v.18 no.1 Performance standards for antimicrobial susceptibility testing; Eighth infornational supplement Ferraro MJ;Craig WA;Eliopoulos G (et al)
  16. BMJ v.302 Association between infection with Helicobacter pylori and risk of gastric cancer, evidence from a prospective investigation Forman D;Newell DG;Fullerton F(et al)
  17. Lancet v.335 Metronidazole resistance in Helicobacter pylori Glupezynski Y;Burette A;Koster E(et al)
  18. J Med Microbiol v.19 Unusual cellual fatty acids and distinctive ultra-structure in a new spiral bacterium (Camphylobacter pyloridis) from the human gastric mucosa Goodwin CS;McCullock RK;Armstron JA;Wee SH
  19. J Clin Pathol v.39 Campylobacter pyloridis, gastritis and peptic ulceration Goodwin CS;Armstrong JA;Marshall BJ
  20. Am J Gastroenterol v.82 Camphylobacter pyloridis gastritis. The past, the present and speculations about the future Graham DY;Klein PD
  21. Gastroenterol v.102 Factors influencing the eradication of Helicobacter pylori with triple therapy Graham DY;Lew GM;Malaty HM(et al)
  22. J Gastroenterol Hepatol v.12 Eradication of Helicobacter pylor. Problems and recommendations Huang JQ;Hunt RH
  23. J Antimicrob Chemother v.43 Antibacterial effect of garlic and omeprazole on Helicobacter pylori Jonkers D;van den Broek E;van Dooren I;Thijs C(et al)
  24. Eur J Epidemol v.9 Evaluation of rapid urease test for detection of Helicobacter pylori in gastric biopsy specimens Kamiya S;Taniguchi I;Yamamoto T(et al)
  25. Eur J Gastroenterol Hepatol v.6 no.SUP. 1 Kamiya S;Kai M;Ozawa A(et al) Characteristics of vacuolating toxin produced Helicobacter pylori
  26. Digestion v.37 no.SUP.2 Antimicrobial action of bismuth in relation to Campylobacter pyloridis conlonization and gastritis Marshall BJ;Armston JA;Fracis GJ (et al)
  27. N Engl J Med v.325 Helicobacter pylori infection and gastric carcinoma among Japanese Americans in Hawai Nomura A;Stemmerman GN;Chyou PH(et al)
  28. N Engl J Med v.325 Helicobacter pylori infection and the risk of gastic carcinoma Parsonet J;Fiedman GD;Vandersteen DP(et al)
  29. J Clin Microbiol v.35 Comparative evluation of the E test, agar dislution, and broth microdilution of testing susceptibilities of Helicobacter pylori strains to 20 antimicrobial agents Piccolomini R;Bonaventura G;Catamo G(et al)
  30. Antimicrob Agents Chemother v.40 In vitro antibacterial activity of omeprazole annd its selectivity for Helicobacter spp. are dependent on incubationconditions Sjoestroem Je;Fryklund J;Huhler T;Larsson H
  31. L Clin Microbiol v.33 Cytotoxin production by Helicobacter pylori from patients with upper gastrointestinal tract diseases Tee W;Lambert JR;Dowyer B
  32. Basic mechanism to clinical cure Helicobacter pylori eradication. Helicobacter pylori. Tytgat GNJ;Noach LA;Hunt RH(ed.);Tytagat GNJ(ed.)
  33. J Clin Microbiol v.34 Metronidazole and clarithromycin resistance in Helicobacter pylori determined by measuring MICs of antimicrobial agents is color indicator egg york agar in a miniwell format Vasquez A;Valdez Y;Giman RH (et al)
  34. Lancet v.I Unidentified curved bacilli on gastric ephitherlium in active chronic gastritis Warren TR;Marshall B
  35. J Clin Microbiol v.34 Heterogeneity in susceptibility to metronidazole among Helicobacter pylori isolates from patient with gastritis or peptic ulcer disease Weel JFL;Hulst RWM;Gerrits Y (et al)
  36. Lancet v.338 Helicobacter pylori-associated gastritis and primary B-cell lymphoma Wotherspoon AC
  37. Helicobacter pyloriの 最新 知見 Helicobacter pyloriの治療總論 佐藤貴一;龍本拓哉;木平 健 (等)
  38. Helicobacter pylori感染症 各種藥濟のHelicobacter pyloriに對する抗菌活性 藤岡利生;那順 藤;藤岡利生