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Gastroepiploic Artery Preservation with Glycerol as a Training Model for Microvascular Anastomosis  

Park, Chan Young (Department of Plastic and Reconstructive Surgery, Research Institute of Plastic and Reconstructive Surgery, Seoul National University College of Medicine)
Chang, Hak (Department of Plastic and Reconstructive Surgery, Research Institute of Plastic and Reconstructive Surgery, Seoul National University College of Medicine)
Chung, Jin Haeng (Department of Pathology, Research Institute of Plastic and Reconstructive Surgery, Seoul National University College of Medicine)
Heo, Eun Ju (Department of Plastic and Reconstructive Surgery, Research Institute of Plastic and Reconstructive Surgery, Seoul National University College of Medicine)
Minn, Kyung Won (Department of Plastic and Reconstructive Surgery, Research Institute of Plastic and Reconstructive Surgery, Seoul National University College of Medicine)
Yoo, Moon Won (Department of Surgery, Research Institute of Plastic and Reconstructive Surgery, Seoul National University College of Medicine)
Yang, Han Kwang (Department of Surgery, Research Institute of Plastic and Reconstructive Surgery, Seoul National University College of Medicine)
Publication Information
Archives of Plastic Surgery / v.35, no.5, 2008 , pp. 626-629 More about this Journal
Abstract
Purpose: We investigate the possibility of vessel preservation with glycerol and evaluate the potential of preserved gastroepiploic artery as a tool for the microsurgical practice. Methods: In 5 early gastric cancer patients, IRB(No. C-0603-040-170), we gained gastroepiploic artery specimens(5 segments, about 10 cm) after gastrectomy. Each segment was rinsed in a normal saline and subsequently placed in a bottle, containing 50 mL anhydrous glycerol (87%). The bottles were refrigerated at $4^{\circ}C$, the longest preservation time being 5 months. Results: At first glance, no fragmentation was detected and the surface of vessel seemed mild sclerotic. In histological examination, vascular structures remained intact though preservation with glycerol led to a mild atrophy of the smooth muscle in the tunica media. Especially, we found out the elastic fibers and endothelial lining of the intima were preserved until 5 months. Adequate status for using microsurgical practice was also maintained and leakage test was performed successfully with gentian violet ink. Conclusion: Based on the results obtained in this study, the gastroepiploic artery preservation with glycerol as a training model for microvascular anastomosis is a technically very simple procedure and useful for the novice microsurgeon.
Keywords
Gastroepiploic artery; Vessel preservation; Glycerol; Microvascular anastomosis;
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Times Cited By KSCI : 1  (Citation Analysis)
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1 Crosby NL, Clapson JB, Buncke HJ, Newlin L: Advanced non-animal microsurgical exercises. Microsurgery 16: 655, 1995   DOI   ScienceOn
2 Waterhouse N, Moss AL, Townsend PL: The development of a dynamic model for microvascular research and practice using human placenta: a preliminary report. Br J Plast Surg 38: 389, 1985   DOI   ScienceOn
3 Kwon SS, Jeong JH, Chang H, Minn KW: Training of microanastomosis with chicken wing brachial artery. J Korean Soc Plast Reconstr Surg 34: 274, 2007
4 Bao JY: Rat tail: a useful model for microvascular training. Microsurgery 16: 122, 1995   DOI   ScienceOn
5 Wolff KD, Dienemann D: Vessel preservation with glycerol: an experimental study in rats. J Oral Maxillofac Surg 48: 914, 1990   DOI   ScienceOn
6 Hino A: Training in microvascular surgery using a chicken wing artery. Neurosurgery 52: 1495, 2003
7 Bishop AJ, Glasby MA, Houlton JE: A morphological assessment of vein allografts preserved in glycerol and used for arterial replacement. J Cardiovasc Surg 28: 491, 1987
8 Lannon DA, Atkins JA, Butler PE: Non-vital, prosthetic, and virtual reality models of microsurgical training. Microsurgery 21: 389, 2001   DOI   ScienceOn