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Toxicogenomics Study on TK6 Human Lymphoblast Cells Treated with Mitomycin C  

Kim, Joo-Hwan (Department of Toxicological Researches, National Institute of Toxicological Research, Korea Food and Drug Administration)
Koo, Ye-Mo (Department of Toxicological Researches, National Institute of Toxicological Research, Korea Food and Drug Administration)
Lee, Woo-Sun (Department of Toxicological Researches, National Institute of Toxicological Research, Korea Food and Drug Administration)
Suh, Soo-Kyung (Department of Toxicological Researches, National Institute of Toxicological Research, Korea Food and Drug Administration)
Kang, Jin-Seok (Department of Toxicological Researches, National Institute of Toxicological Research, Korea Food and Drug Administration)
Han, Eui-Sik (Department of Toxicological Researches, National Institute of Toxicological Research, Korea Food and Drug Administration)
Kim, Seung-Hee (Department of Toxicological Researches, National Institute of Toxicological Research, Korea Food and Drug Administration)
Park, Sue-N. (Department of Toxicological Researches, National Institute of Toxicological Research, Korea Food and Drug Administration)
Publication Information
Molecular & Cellular Toxicology / v.3, no.3, 2007 , pp. 165-171 More about this Journal
Abstract
Mitomycin C (MMC), an antitumor antibiotic isolated from Streptomyces caespitosus, is used in chemotherapy of gastric, bladder and colorectal cancer. MMC is activated in vivo to alkylate and crosslink DNA, via G-G interstrand bonds, thereby inhibiting DNA synthesis and transcription. This study investigates gene expression changes in response to MMC treatment in order to elucidate the mechanisms of MMC-induced toxicity. MMC was admistered with single dose (0.32 and 1.6 ${\mu}M$) to TK6 cells. Applied Biosystem's DNA chips were used for identifying the gene expression profile by MMC-induced toxicity. We identified up- or down-regulated 90 genes including cyclin M2, cyclin-dependent kinase inhibitor 1A (p21, cip1), programmed cell death 1, tumor necrosis factor (ligand) superfamily, member 9, et al. The regulated genes by MMC associated with the biological pathways apoptosis signaling pathway. Further characterization of these candidate markers related to the toxicity will be useful to understand the detailed mechanism of action of MMC.
Keywords
Toxicogenomics; Mitomycin C; Microarray; Gene expression;
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