• Parasites, Hosts and Diseases
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• v.52 no.6
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• pp.653-659
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• 2014

Surgery remains the preferred treatment for hydatid cyst (cystic echinococcosis, CE). Various scolicidal agents have been used for inactivation of protoscolices during surgery, but most of them are associated with adverse side effects. The present study aimed to evaluate the in vitro scolicidal effect of Nigella sativa (Ranunculaceae) essential oil and also its active principle, thymoquinone, against protoscolices of hydatid cysts. Protoscolices were aseptically aspirated from sheep livers having hydatid cysts. Various concentrations of the essential oil (0.01-10 mg/ml) and thymoquinone (0.125-1.0 mg/ml) were used for 5 to 60 min. Viability of protoscolices was confirmed by 0.1% eosin staining. Furthermore, the components of the N. sativa essential oil were identified by gas chromatography/mass spectroscopy (GC/MS). Our study revealed that the essential oil of N. sativa at the concentration of 10 mg/ml and its main component, thymoquinone, at the concentration of 1 mg/ml had potent scolicidal activities against protoscolices of Echinococcus granulosus after 10 min exposure. Moreover, thymoquinone (42.4%), p-cymene (14.1%), carvacrol (10.3%), and longifolene (6.1%) were found to be the major components of N. sativa essential oil by GC/MS analysis. The results of this study indicated the potential of N. sativa as a natural source for production of a new scolicidal agent for use in hydatid cyst surgery. However, further studies will be needed to confirm these results by checking the essential oil and its active component in in vivo models.

• Korean Journal of Food Science and Technology
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• v.26 no.5
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• pp.512-519
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• 1994

To investigate the effects of cultivation on the aroma characteristics of essential oil of peppermint (Mentha piperita L.), experimental peppermints were cultivated with different treatments of field mulching, soil fertilization, and harvesting time. Aroma characteristics of the different mint oils were analyzed by gas chromatography (GC) and GC/mass spectrometry (MS)/infrared spectroscopy (IR). Growth of peppermint with vinyl muching was faster in the early stage of cultivation, but became almost same in the harvest date. The higher amounts of rainfall and sunshine hour resulted in the better growth of stem and leaf of the peppermint. Contents of menthone in the essential oils from the peppermint cultivated in 1990 increased as harvest date delayed from late June 25 to late July 26 the same period. In 1991 menthone decreased from June 25 to August 5, but l-menthol increased from June 25 to august 5. The results indicated that the sunshing hour affected biosynthesis of the components. Contents of menthofuran increased significantly from early June to late July. The optimum cutting date resulting best oil production was late July 7 in 1991.

• Journal of the Korea Organic Resources Recycling Association
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• v.16 no.4
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• pp.57-63
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• 2008

The characteristics of the bio-oil produced by the pyrolysis process with pig feces was investigated in this paper. The continuous auger-type reactor produced bio-oil was maintained at the temperature range of 400 to $600^{\circ}C$, which was higher than a typical that in a conventional pyrolysis system. The pig feces was used as the feedstock. The bio-oil and its compositions were characterized by water analysis, heating values, elemental analysis, bio-oil compounds, by Gas Chromatography/Mass Spectrometry (GC/MS), and functional group by $^1H$ NMR spectroscopy. It was found that the maximum bio-oil yields of 21% w.t. was achieved at $550^{\circ}C$. This result suggested that this auger reactor might be a potential technology for livestock waste treatment to produce bio-oil because it is able to be improved to reach higher efficiency of bio-oil production in further study. The pyrolysis system reported herein had low heat transfer into the feedstock in the auger reactor so that it needs improve the heat conduction rate of the system in further study.

• Journal of Microbiology and Biotechnology
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• v.26 no.2
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• pp.248-254
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• 2016

A soil metagenome contains the genomes of all microbes included in a soil sample, including those that cannot be cultured. In this study, soil metagenome libraries were searched for microbial genes exhibiting lipolytic activity and those involved in potential lipid metabolism that could yield valuable products in microorganisms. One of the subclones derived from the original fosmid clone, pELP120, was selected for further analysis. A subclone spanning a 3.3 kb DNA fragment was found to encode for lipase/esterase and contained an additional partial open reading frame encoding a wax ester synthase (WES) motif. Consequently, both pELP120 and the full length of the gene potentially encoding WES were sequenced. To determine if the wes gene encoded a functioning WES protein that produced wax esters, gas chromatography-mass spectroscopy was conducted using ethyl acetate extract from an Escherichia coli strain that expressed the wes gene and was grown with hexadecanol. The ethyl acetate extract from this E. coli strain did indeed produce wax ester compounds of various carbon-chain lengths. DNA sequence analysis of the full-length gene revealed that the gene cluster may be derived from a member of Proteobacteria, whereas the clone does not contain any clear phylogenetic markers. These results suggest that the wes gene discovered in this study encodes a functional protein in E. coli and produces wax esters through a heterologous expression system.

• Korean Journal of Food Science and Technology
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• v.46 no.4
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• pp.418-424
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• 2014

High-acidity vinegar was manufactured using pear concentrate by fed-batch fermentation without additional nutrients, and the physicochemical properties and volatile components were investigated at different fermentation stages (Stages 1-4) and at various initial alcohol concentrations (IAC; 6-9%). The levels of reducing sugar, free amino acids, total phenolic content, total flavonoid content, and radical scavenging ability increased slightly during Stage 4 (high-acidity vinegar), which was affected by alcohol feeding. The contents of approximately 20 types of volatile compounds differed between the moderate- and high-acidity vinegar samples, as determined by solid-phase microextraction/gas chromatography-mass spectroscopy. The level of acetic acid in high-acidity vinegar increased according to the initial alcoholic content applied. The high-acidity vinegar produced by fed-batch culture at an IAC of 6-7% showed improved physicochemical and volatile properties as compared to the moderate-acidity vinegar.

• Environmental Engineering Research
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• v.23 no.3
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• pp.294-300
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• 2018

Cometabolism technology was employed to degrade lignin wastewater in Sequencing Batch Biofilm Reactor. Cometabolic system (with glucose and lignin in inflow) and the control group (only lignin in inflow) were established to do a comparative study. In contrast with the control group, the average removal rates of lignin increased by 14.7% and total oarganic carbon increased by 32% in the cometabolic system with glucose as growth substrate, under the condition of 5 mg/L DO, $0.2kgCOD/(m^3{\cdot}d)$ lignin and glucose $1.0kgCOD/(m^3{\cdot}d)$. Functional groups of lignin are degraded effectively in cometabolic system proved by fourier transform infrared spectroscopy and Gas Chromatography-Mass Spectrometer, and the degradation products were amides (mainly including acetamide, N-ethylacetamide and N, N-diethylacetamide), alcohols (mainly including glycerol and ethylene glycol) and acids. Meanwhile, results of Polymerase Chain Reaction-Denaturing Gradient Gel Electrophoresis showed great differences in microbial population richness between cometabolic system and the control group. The Margalef's richness index and Shannon-Wiener's diversity index of microorganism in cometabolic system were 3.075 and 2.61, respectively. The results showed that extra addition of glucose, with a concentration of 943 mg/L, was beneficial to lignin biodegradation in cometabolic system.

• Journal of Adhesion and Interface
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• v.20 no.3
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• pp.102-109
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• 2019

The material constitution of multi-layered thin film coated on the PET base film was analyzed using ATR FT-IR and pyro GC/MS combination. The cross section of the film was acquired by cracking the film after dipping in liquid nitrogen and was observed using optical microscope. Total thickness of the coated film was $70{\mu}m$ and three layers were observed. Since each layers were too thin to analyze directly except the surface layer, analyzable area of each layers were exposed by using a proper solvent and were investigated using ATR FT-IR and pyro GC/MS. Results shows that three layers were commonly consisted of urethane-acrylate copolymers. Also, inorganic and/or metal inclusions detected by XPS and SEM-EDAX were exhibited by nano size $SiO_2$ particles in layer(1) and aluminum flakes in layer(2).

• Korean Journal of Agricultural Science
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• v.46 no.1
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• pp.33-44
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• 2019

This study was conducted to examine the components of phytoncide from a pine forest in the southern temperate zone. Recent studies have found that a large amount of phytoncide is released not only from cypress trees but also from pine trees. Because the amount released is the highest during summer, we selected a warm climate region in the southern temperate zone and measured the concentration in the month of August. To capture the phytoncide from the forest atmosphere, we used the adsorption tube method with a mini pump and successfully gathered 9 L of forest air at a flow rate of 150 mL/min. We performed duplicate sampling from two different tubes installed at the same location and derived the mean value. A gas chromatography/mass spectrometer detector with thermal desorption spectroscopy was utilized to perform quantitative and qualitative analyses of the captured material. The results showed that the average phytoncide particle of the pine forest in the southern temperate zone contained a number of components as follows in descending order: ${\alpha}$-Pinene (39%, $0.28ng/m^3$), followed by ${\beta}$-Pinene (16%, $0.11ng/m^3$), D-Limonene (8%, $0.06ng/m^3$), camphor (6%, $0.04ng/m^3$), camphene (6%, $0.04ng/m^3$), and p-Cymene (5%, $0.04ng/m^3$). There were also 13 additional phytoncide components in trace amounts. The results of this study are expected to provide a useful dataset for building a "Healing-forest".

• Mycobiology
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• v.49 no.1
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• pp.78-85
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• 2021

Morels (Morchella spp.) are valuable medicinal and edible mushrooms. In this study, chemical profiles of aromas and lipophilic extracts of black morel (Morchella importuna) grown in China were analyzed by gas chromatography/mass spectroscopy, along with the evaluation of antioxidant and antimicrobial activities for the lipophilic extracts. Sixty-five compounds in total were identified from the aromas, and 1-octen-3-ol was the main component for aromas of fresh (34.40%) and freeze-dried (68.61%) black morels, while the most abundant compound for the aroma of the oven-dried sample was 2(5H)-furanone (13.95%). From the lipophilic extracts, 29 compounds were identified with linoleic acid as the main compound for fresh (77.37%) and freeze-dried (56.46%) black morels and steroids (92.41%) as the main constituent for an oven-dried sample. All three lipophilic extracts showed moderate antioxidant activities against 2,2-diphenyl-1-picrylhydrazyl and 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) with the IC50 values ranging 7.56~17.52 mg/mL and 5.75~9.73 mg/mL, respectively, and no obvious antimicrobial activity was observed for lipophilic extracts. The drying methods affect the chemical profile of black morel, and freeze-drying was favorable for retaining nutrients and morel smell. This is the first report on the aroma and lipophilic extracts of M. importuna grown in China.

• Journal of the Korean Wood Science and Technology
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• v.43 no.4
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• pp.494-503
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• 2015

This study was focused on the comparison of the variations in the yield and chemical composition of Chamaecyparis obtusa leaf oil obtained under different pressure conditions of the supercritical carbon dioxide extraction (SCE), and by hydro-distillation. SCE was carried out varying the pressure in the range of 100~400 bar at $40^{\circ}C$. The chemical composition of C. obtusa leaf oils was determined by gas chromatography-mass spectroscopy (GC-MS) analysis. The maximum yield of 4.4% (relative to the initial mass of oven dry mass) was obtained in the extraction under 300 bar pressure, which was higher than that of the hydro-distillation method (1.9%). The contents of sesquiterpenes in the extracts obtained by the SCE were higher than those of the essential oils of C. obtusa by the hydro-distillation. The sesquiterpenes in the SCE extracts made up approximately 39%~46% of the total, followed by monoterpenes, diterpene, and lignan. The contents of each constituent in the supercritical carbon dioxide extracts were varied on the extraction pressure. Therefore, these results showed that the extraction condition of SCE had significant effect on the yield of C. obtusa oils and its chemical composition.