• Korean Journal Plant Pathology
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• v.14 no.6
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• pp.594-597
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• 1998

The optimal quantity of inoculum was determined to screen resistance of garlic cultivars against Sclerotium cepivorum and 30 cultivars was tested. The growth of the pathogen in detached roots, Disease incidence was increased when the inoculum density was raised form 10 to 100 sclerotia. The optimal inoculum density to differentiate resistance or susceptibility of garlic cultivars was seemed to be 50 sclerotia. The cultivars collected from England, Japan, Nepal and Turkey, and cultivars such as common red, PI1356104 and PI135693 were less than the other cultivars in their disease incidence. The growth of S. cepivorum in detached roots varied from 23 to 33 mm according to garlic cultivars. There was no relationship between the disease incidence and the growth in detached roots. The sclerotial germination was increased significantly when root extract was extract was added. The addition of only distilled water resulted in 13% germination, but the addition of 0.25 g of root extract in 100 ml distilled water resulted in more than 85% germination. There was no difference in the stimulation of sclerotial germination among cultivars which showed different resistance.

• Korean Journal of Plant Resources
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• v.23 no.5
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• pp.458-464
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• 2010

Twelve garlic cultivars collected from Korea and Mongolia were evaluated genetic similarity and diversity by RAPD method using oligo-nucleotide random primers. Genomic DNA isolated from twelve garlic cultivars were amplified by polymerase chain reaction using 143 primers, and 55 primers showed polymorphic DNA bands. Among a total of 187 bands amplified by 55 primers, 128 polymorphic bands were subjected to analysis for genetic relationship of garlic cultivars. Garlic cultivars were classified into three groups, such as group-I corresponded to Euiseong, Seosan, Samchuk and Yecheon-A, Yechun-B, Euiseong-norang, Jeongsun, Namdo, Yookback and Danyang cultivars, and group-II to Mongolia and group-III to Daeseo cultivars. Thirty DNA bands showing unique specificity to the specific cultivars are likely to be useful for identification of garlic local cultivars as DNA markers.

• Korean Journal of Plant Resources
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• v.27 no.1
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• pp.35-42
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• 2014

Correlation and similarity of garlic cultivars was analyzed by measuring the composition of twenty amino acids contents in the bulb and callus from twelve garlic cultivars which were collected from Korea and foreign countries. Arginine and asparagine occupied more than 78% of total amino acids both in bulb and callus followed by glutamic acid, lysine, aspartic acid, valine, glycine, histidine and ornithine in order. Based on correlation coefficients of amino acid composition in the bulb, twelve garlic cultivars were classified into three major groups. Danyang, Euiseong, Seosan, Jeju, Mongol and Turkey cultivars belong to group-I, Namdo, China, Mexico and Nepal cultivars are in group-II, and group-III includes Philippine and Daeseo cultivars. Based on amino acid composition in callus, group-I includes Mongol, Euiseong, Danyang and Seosan cultivars, group-II includes Jeju, Daeseo, China and Namdo cultivars, group-III includes Napal, Mexico, Philippine and Turkey cultivars. Composition of amino acid contained in both callus and bulb is a clear standard to identify northern-type garlic cultivars. Especially the composition of amino acids in callus is more distinctive standard of classification between northern and southern type garlic cultivars than that in bulbs.

• Journal of Life Science
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• v.9 no.6
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• pp.671-676
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• 1999

RAPD analysis using random primers were tried to evaluate the genetic variation and diversity of the nine garlic cultivars including two foreign varieties. Thirty-two primers out of 70 primers screened were used to amplify genomic DNA of garlic cultivars using polymerase chain reaction(PCR). Among a total of 151 bands amplified by 32 primers, 125 polymorphic bands were subjected to analysis for genetic relationship of garlic cultivars. The estimated size of amplified PCR products were in the range of 932 to 4,060 base pairs. Nine garlic cultivars were classified into two groups, such as group I corresponded to Changnyung and Hungary cultivars, and group II, Namdo, Sandong from China, Yecheon, Euiseong, Youngweol, Danyang, Jeongsun cultivars, with the genetic distance value of 0.271. The major ecological types of garlics, so called southern and northern types, was grouped in the genetic distance value of 0.200. The results presented in this study suggest that RAPD analysis are likely to be useful for identification of cultivars and evaluation of genetic origin in garlics.

• Korean Journal of Plant Resources
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• v.24 no.5
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• pp.584-590
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• 2011

Wound inducible P450-Esg cDNA, one of cytochrome P450 gene family, was isolated from shoot of Euiseong garlic cultivar. P450-Esg cDNA possesses highly conserved heme-binding domain in the nucleotide sequence, and 1,419 bp of open reading frame (ORF) coding of 473 amino acids. Based on the nucleotide sequence analysis of P450-Esg homologous from twelve garlic cultivars, two domains, one domain between 472 to 510 bp, and the other between 1,210 to 1,249 bp from start codon (ATG), showed various nucleotide polymorphism among cultivars. Sequence of heme-binding domain in P450-Esg homologous, which is located at the domain between 1,210 to 1,240 bp from start codon, showed various nucleotide polymorphism as well as amino acid sequence polymorphism among twelve garlic cultivars. Anther domain, between 472 to 510 bp from start codon, showed exactly same amino acid sequence in the twelve garlic cultivars, but there were various single nucleotide polymorphism to the cultivars.

• Research in Plant Disease
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• v.9 no.3
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• pp.121-124
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• 2003

Occurrence of rust on 2 representative garlic cultivars Daeseo and N므애 in major cultivating regions of Goheung-gun, Jeonnam and Namhae-gun, Gyeongnam was investigated during 1998-1999. Rust disease severities of garlic ranging from 1.2 to 85.8 were quite different according to the investigating years and cultivars. Relatively high rust severities of garlic were observed in 1998 compared with 1999. This might be due to remarkably frequent rainfalls and high humidities during the growing seasons of garlic in 1998. Rust occurred severely on cultivar Daeseo but not on cultivar Namdo. This shows quite different susceptibility or resistance to rust among the cultivars of garlic. Increase of rust severities resulted in remarkable decrease in yields of garlic. It is assumed that rust might cause serious damages in the cultivation of garlic.

• Research in Plant Disease
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• v.10 no.4
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• pp.349-352
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• 2004

Antimicrobial activity of garlic extracts of four local cultivars (Namdo, Goheung, Danyang and garlic imported from China) against several plant pathogenic bacteria and fungi was investigated. The garlic extracts exhibited marked in vitro antimicrobial activities against the Gram-positive and -negative bacteria as well as fungal pathogens. Of the four cultivars, two cultivars (Namdo and garlic imported from China) showed relatively good antimicrobial activities. Gram-positive bacteria, Clavibater michiganensis was most sensitive, whereas Burkholderia cepacia was the strong resistant towards garlic extracts. Results from the planta bioassays under greenhouse conditions indicated that garlic extracts significantly reduced the cucumber powdery mildew disease showing control value 59.7-61.9% compared with the nontreated controls.

• Korean Journal of Plant Resources
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• v.31 no.5
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• pp.531-537
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• 2018

This study was carried to survey distribution of the nucleotide polymorphisms in heme-binding (HB) domain, which is highly conserved region between 1,210 and 1,240 bp of cytochrome P450, in domestic garlic cultivars. 120 garlic cultivars collected from Korea were classified into seven HB domain variation based on the nucleotide sequence of the domain. Northern type garlic cultivars, collected from Kyungpook, Chungnam, Chungpook and Kangwon province, showed 51.3% of KP2 type nucleotide sequence, 5'-TTT/GGC/GGT/GGA/CGG/AGA/ATA/TGT/CCT/GGA-3' with coding amino acid FGGGRRICPG, 13.7% of KP1, 11.3% of CP, 8.8% of CM and 5% of KW2 types. Southern type cultivars, collected from Kyungnam province, showed 52.5% of KM type nucleotide sequence, 5'-TTT/GGC/GCA/GGA/CGG/AGA/ATT/TGT/CCT/GGA-3' with coding amino acid FGAGRRICPG, 22.5% of KP2, 5.0% of KW2 and 2,5% of CP type nucleotide sequence. These results showed that Korean garlics were cultivated in highly mixed condition even in the same region.

• Proceedings of the Korean Society of Plant Biotechnology Conference
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• 2002.04a
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• pp.33-40
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• 2002

We, Tong Yang Moolsan Co. Ltd. (TYM) set up the mass-production system for virus-free seed garlic via tissue culture technique. TYM's tissue culture technique is called as 'Multiple shoot propagation technique'. This technique can lead mass propagation of genetically homogeneous seed garlic in a short period because of its highly proliferation rate of in vitro shoots ($15^{10}$ /year). TYM researchers applied the technique to some selected garlic cultivars with superior characteristics and carried out field test of productivity in the inside and outside of the country for several years. According to the yearly results of field test with virus-free seed garlic, we ascertained that virus-free seed garlic can produce the highly yield increase (max. above $50\%$) and also can enhance the product quality. Consequently, we estimated that TYM's seed garlic will contribute to farmers with increase of income and can elevate the national position of garlic market in the world for its competitive power of technical and production cost.

• Journal of agriculture & life science
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• v.46 no.5
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• pp.91-100
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• 2012

Physicochemical and antimicrobial activities of 12 different garlic cultivars were investigated. Width and weight of California late cultivar (60.44mm, 53.73g) was the biggest and heaviest but Changyoung cultivar (44.04mm, 25.15g) was the smallest and lightest among the variety of garlic. The range of L, a and b color characteristics of garlic surface from different variety were 84.13~90.56, -1.10~0.77 and 18.24~26.61, respectively. Shear force was the lowest in California early, but 94-12-2 cultivar ($4211.35cm/kg^2$) was higher than another cultivars. Soluble solid range was 6.40~11.33 %brix, and Changyoung cultivar was the highest than the others, significantly. pH of garlics from different cultivar were 5.57~6.53. Total thiosulfinate content of California late cultivar (146.05mM/g) was higher, but Italy cultivar (93.23mM/g) was lower than the others. Total pyruvate content was the highest in Yugo cultivar ($162.50{\mu}M/g$) and the lowest in California early cultivar ($147.41{\mu}M/g$).