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Genetic Variation and Identification of RAPD Markers from Some Garlic Cultivars in Korea and Mongolia  

Bae, Seong-Kuk (Dept. of Horticulture and Breeding, Andong Nat'l University)
Jung, Eun-A (Dept. of Horticulture and Breeding, Andong Nat'l University)
Kwon, Soon-Tae (Dept. of Horticulture and Breeding, Andong Nat'l University)
Publication Information
Korean Journal of Plant Resources / v.23, no.5, 2010 , pp. 458-464 More about this Journal
Abstract
Twelve garlic cultivars collected from Korea and Mongolia were evaluated genetic similarity and diversity by RAPD method using oligo-nucleotide random primers. Genomic DNA isolated from twelve garlic cultivars were amplified by polymerase chain reaction using 143 primers, and 55 primers showed polymorphic DNA bands. Among a total of 187 bands amplified by 55 primers, 128 polymorphic bands were subjected to analysis for genetic relationship of garlic cultivars. Garlic cultivars were classified into three groups, such as group-I corresponded to Euiseong, Seosan, Samchuk and Yecheon-A, Yechun-B, Euiseong-norang, Jeongsun, Namdo, Yookback and Danyang cultivars, and group-II to Mongolia and group-III to Daeseo cultivars. Thirty DNA bands showing unique specificity to the specific cultivars are likely to be useful for identification of garlic local cultivars as DNA markers.
Keywords
PCR; RAPD; polymorphic bands; identification of cultivars;
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