Browse > Article
http://dx.doi.org/10.7732/kjpr.2018.31.5.531

Nucleotide Polymorphisms of Cytochrome P450 Genes in Domestic Garlic Cultivars  

Kwon, Soon-Tae (Department of Horticulture and Breeding, Andong Nat'l University)
Chung, Jinbo (Department of Horticulture, Purdue University)
Publication Information
Korean Journal of Plant Resources / v.31, no.5, 2018 , pp. 531-537 More about this Journal
Abstract
This study was carried to survey distribution of the nucleotide polymorphisms in heme-binding (HB) domain, which is highly conserved region between 1,210 and 1,240 bp of cytochrome P450, in domestic garlic cultivars. 120 garlic cultivars collected from Korea were classified into seven HB domain variation based on the nucleotide sequence of the domain. Northern type garlic cultivars, collected from Kyungpook, Chungnam, Chungpook and Kangwon province, showed 51.3% of KP2 type nucleotide sequence, 5'-TTT/GGC/GGT/GGA/CGG/AGA/ATA/TGT/CCT/GGA-3' with coding amino acid FGGGRRICPG, 13.7% of KP1, 11.3% of CP, 8.8% of CM and 5% of KW2 types. Southern type cultivars, collected from Kyungnam province, showed 52.5% of KM type nucleotide sequence, 5'-TTT/GGC/GCA/GGA/CGG/AGA/ATT/TGT/CCT/GGA-3' with coding amino acid FGAGRRICPG, 22.5% of KP2, 5.0% of KW2 and 2,5% of CP type nucleotide sequence. These results showed that Korean garlics were cultivated in highly mixed condition even in the same region.
Keywords
Cyt. P450; Ecotype; Garlic cultivar; Heme binding domain; Polymorphism;
Citations & Related Records
Times Cited By KSCI : 1  (Citation Analysis)
연도 인용수 순위
1 Kwon, S.T. and J. Kamiya. 2011. Genetic variation of cytochrome P450 genes in garlic cultivars. Korean J. Plant Res. 24:584-590 (in Korean)   DOI
2 Kwon, S.T. and S.M. Oh. 1999. Genetic relationship among garlic cultivars based on RAPD analysis. Korean J. Life Sci. 9:671-676 (in Korean).
3 Lee, W.S. 1974. Studies on dormancy of Korean local garlics. J. Korean Soc. Hort. Sci. 15:257-264 (in Korean).
4 Morita, A., T. Nakayama, N. Doba, S. Hinohara, T. Mizutani, M. Soma. 2007. Genotyping of triallelic SNPs using TaqMan PCR. Molecular and Cellular Probes 21:171-176.   DOI
5 Ralston, L., S.T. Kwon, M. Schoenbeck, J. Ralston, D.J. Schnek, R.M. Coates and J. Chappell. 2001. Cloning, heterologous expression, and functional characterization of 5-epi-aristolochene-1,3-dihydroxylase from tobacco (Nicotiana tabacum). Arch. Biochem. Biophys. 393:222-235.   DOI
6 Bae, S.K., E.A. Jung and S.T. Kwon. 2010. Genetic variation and identification of RAPD markers from some garlic cultivars in Korea and Mongolia. Korean J. Plant Res. 23:458-464 (in Korean).
7 Boo, D. and S.J. Park. 2016. Molecular phylogenetic study of Korean Tilia L. Korean J. Plant Res. 29:547-554 (in Korean)   DOI
8 Cheshmedhiev, I. 1973. A cytotaxonomic investigation of the cultivated Allium species in Bulgaria. Genetikai Selektsiya 6:283-294.
9 Donaldson, R.P. and D.G. Luster. 1991. Multiple forms of plant cytochrome P450. Plant Physiol. 96:669-674.   DOI
10 Drabovich, A. P and S. Krylov. 2006. Identification of base pairs in single-nucleotide polymorphisms by MutS protein-mediated capillary electrophoresis. Anal. Chem. 78:2035-2038.   DOI
11 Harn, C., D. Choung and B. Kim. 1996. Studies on the karyotypes of Allium sativum. J. Korean Soc. Hort. Sci. 2:58-67.
12 Hoshino, T., T. Yamaura, H. Imaishi, M. Chida, Y. Yoshizawa, K. Higashi, H. Ohkawa and J. Mizutani. 1995. 5-epi-Aristolochene 3-hydroxylase from green pepper. Phytochemistry 38:609-613.   DOI
13 Hwang, J.M. 1993. Genetic divergence and classification of garlic cultivars by multivariate analysis. J. Korean Soc. Hort. Sci. 33:257-264 (in Korean).
14 Kollmann, F. and A. Shmida. 1977. Allium species of Mt. Hermon. I. Taxonomy. Israel J. Botany 26:128-148.
15 Kwon, S.T., J. Chappell. 1998. Elicitor-inducible 5-epi-aristolochene hydroxylase in suspension cultures of tobacco (Nicotiana tabacum L.) Korean J. Plant Tiss. Cult. 25:141-146.