• Journal of Radiation Protection and Research
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• 제28권3호
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• pp.255-261
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• 2003

환경선량변동을 실시간으로 모니터링하기 위해서 원자력연구소 부지 주변에 온라인 감시시스템이 운영중인데, 현장에 설치된 전리함 검출기에 의해서 측정된 자료는 모뎀을 통해 무선전송방식으로 중앙제어실에 실시간으로 저장된다. 무선 송수신 방식은 전송, 처리 및 저장에 경제적이고 효과적이지만 통신 장애시 현장감시장치에 저장된 백업데이터를 가져오지 못해서 결국 데이터가 손실될 우려가 있다 물론 버퍼에 저장된 선량값을 디스켓 및 다른 off-line으로 복원이 가능하지만 이럴 경우 온라인으로 운영되는 시스템에 과거데이터를 처리해야 하므로 복잡하고 실시간으로 처리 할 수가 없다. 본 연구에서는 기존 감시시스템 중 신호처리 부분과 운영 소프트웨어를 새롭게 개발해서, 현장에 설치된 감시기와 중앙처리 장치의 시각을 매일 자동으로 동기화시키고 이를 이용해서 현장감시장치에 저장된 백업데이터를 자동으로 복원시켰다. 새로 개발된 방식을 이용할 경우 34시간이내에서 통신장애로 수신하지 못한 백업데이터를 자동으로 복원할 수 있고, 복원된 데이터를 현재시각의 데이터와 함께 실시간으로 디스플레이 할 수 있게 하였다.

• Asian Pacific Journal of Cancer Prevention
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• 제14권6호
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• pp.3669-3676
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• 2013

Faithful transmission of genetic information depends on accurate chromosome segregation as cells exit from mitosis, and errors in chromosomal segregation are catastrophic and may lead to aneuploidy which is the hallmark of cancer. In eukaryotes, an elaborate molecular control system ensures proper orchestration of events at mitotic exit. Phosphorylation of specific tyrosyl residues is a major control mechanism for cellular proliferation and the activities of protein tyrosine kinases and phosphatases must be integrated. Although mitotic kinases are well characterized, phosphatases involved in mitosis remain largely elusive. Here we identify a novel variant of mouse protein tyrosine phosphatase containing domain 1 (Ptpcd1), that we named Ptpcd2. Ptpcd1 is a Cdc14 related centrosomal phosphatase. Our newly identified Ptpcd2 shared a significant homology to yeast Cdc14p (34.1%) and other Cdc14 family of phosphatases. By subcellular fractionation Ptpcd2 was found to be enriched in the cytoplasm and nuclear pellets with catalytic phosphatase activity. By means of immunofluorescence, Ptpcd2 was spatiotemporally regulated in a cell cycle dependent manner with cytoplasmic abundance during mitosis, followed by nuclear localization during interphase. Overexpression of Ptpcd2 induced mitotic exit with decreased levels of some mitotic markers. Moreover, Ptpcd2 failed to colocalize with the centrosomal marker ${\gamma}$-tubulin, suggesting it as a non-centrosomal protein. Taken together, Ptpcd2 phosphatase appears a non-centrosomal variant of Ptpcd1 with probable mitotic functions. The identification of this new phosphatase suggests the existence of an interacting phosphatase network that controls mammalian mitosis and provides new drug targets for anticancer modalities.

• 한국정보통신학회논문지
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• 제14권7호
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• pp.1708-1714
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• 2010

본 논문에서는 기존의 full color LED 전광판에서 일반적인 화질 개선 혹은 화질 보정의 방법으로는 감마, 휘도, 명도조절 등으로 영상의 전체적인 색상과는 상관없이 일률적으로 밝게 혹은 어둡게 처리해 왔다. 그러나 단순히 표출되는 영상 자체의 휘도를 일정 크기로 일률적으로 휘도를 조정하는 방법은 입력되는 영상 신호의 특성을 반영하지 않아 단지 화면 전체가 밝아지거나 어두워졌다는 느낌만을 줄 뿐이다. 그래서 기존의 영상 전송 방식과는 다른 새로운 기술로 LED 전광판에 입력되는 영상 데이터의 히스토그램 분석을 통해 기준 휘도 값을 결정함으로써 입력되는 영상 데이터의 특성이 반영되고, 해당 기준 휘도 값에 따라 히스토그램의 분포를 제어함으로써 LED 전광판에 표출되는 영상 휘도를 균일하게 향상시킬 수 있는 히스토그램 분포 제어가 가능한 full color 전광판의 영상 구동 처리기술을 제안하였다.

• The Korean Journal of Physiology and Pharmacology
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• 제14권3호
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• pp.127-132
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• 2010

Reactive oxygen species (ROS), which include hydrogen peroxide ($H_2O_2$), the superoxide anion (${O_2}^-{\cdot}$), and the hydroxyl radical ($OH{\cdot}$), are generated as by-products of oxidative metabolism in cells. The cerebral cortex has been found to be particularly vulnerable to production of ROS associated with conditions such as ischemia-reperfusion, Parkinson's disease, and aging. To investigate the effect of ROS on inhibitory GABAergic synaptic transmission, we examined the electrophysiological mechanisms of the modulatory effect of $H_2O_2$ on GABAergic miniature inhibitory postsynaptic current (mIPSCs) in mechanically isolated rat cerebral cortical neurons retaining intact synaptic boutons. The membrane potential was voltage-clamped at -60 mV and mIPSCs were recorded and analyzed. Superfusion of 1-mM $H_2O_2$ gradually potentiated mIPSCs. This potentiating effect of $H_2O_2$ was blocked by the pretreatment with either 10,000-unit/mL catalase or $300-{\mu}M$ N-acetyl-cysteine. The potentiating effect of $H_2O_2$ was occluded by an adenylate cyclase activator, forskolin, and was blocked by a protein kinase A inhibitor, N -(2-[p-bromocinnamylamino] ethyl)-5-isoquinolinesulfonamide hydrochloride. This study indicates that oxidative stress may potentiate presynaptic GABA release through the mechanism of cAMP-dependent protein kinase A (PKA)-dependent pathways, which may result in the inhibition of the cerebral cortex neuronal activity.

• 대한전자공학회논문지TC
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• 제41권8호
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• pp.19-26
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• 2004

패키지는 전기적인 특성에 있어서 우수한 고주파 전송 특성을 지녀야한다. 그러나 집적회로면이 위로 향하는 세라믹 패키지(Face-up Package)는 본드와이어 연결 시 고주파의 기생 특성이 크게 증가하여 시스템 전체의 성능에 큰 제한을 가져온다. 본 논문에서는 향상된 정합특성을 갖는 새로운 밀리미터파 세라믹 패키지 급전구조를 제안하였고, 유한요소법(FEM: Finite Element Method)을 이용하여 20 ∼ 50 GHz에서 해석 및 설계를 하고 제작하였다. 측정 결과, 삽입된 금속판 (Embedded Metal Sheet)을 가지는 세라믹 패키지 급전구조는 47GHz까지 기존의 세라믹 패키지보다 0.85dB 그리고 본드와이어 부분에 일반적인 에폭시( ε/sub γ/ = 4)를 사용하여 몰딩한 세라믹 패키지보다 0.4dB가 개선된 삽입손실의 특성을 얻을 수 있었다. 따라서 본 해석결과는 소형의 세라믹 패키지 및 MMIC(Monolithic Microwave Integrated Circuit) 모듈 개발에 효과적으로 활용될 수 있으리라 기대된다.

• Asian Pacific Journal of Cancer Prevention
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• 제14권2호
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• pp.747-752
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• 2013

Oncostatin M (OSM) is a multifunctional cellular regulator acting on a wide variety of cells, which has potential roles in the regulation of gene activation, cell survival, proliferation and differentiation. Previous studies have shown that OSM can induce morphological and/or functional differentiation and maturation of many tumor cells. However, the action of OSM on the induction of differentiation of human hepatocellular carcinoma (HCC) has not been reported. Here, we investigated the effects of different concentrations of OSM on human HCC cell line SMMC-7721 growth, proliferation, cell cycling, apoptosis and differentiation in vitro. Cell growth was determined via MTT assay, proliferation by cell cycle analysis, apoptosis by flow cytometry, morphology by transmission electronic microscopy, and cell function by detection of biochemical markers. Our results demonstrated that OSM strongly inhibited the growth of SMMC-7721 cells in a dose-dependent manner, associated with decreased clonogenicity. Cell cycle analysis revealed a decreased proportion of cells in S phase, with arrest at G0/G1. The apotosis rate was increased after OSM treatment compared to the control. These changes were associated with striking changes in cellular morphology, toward a more mature hepatic phenotype, accompanied by significant reduction of the expression of AFP and specific activity of ${\gamma}$-GT, with remarkable increase in secretion of albumin and ALP activity. Taken together, our findings indicate that OSM could induce the differentiation and reduce cell viability of SMMC-7721 cells, suggesting that differentiation therapy with OSM offers the opportunity for therapeutic intervention in HCC.

• 한국재료학회지
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• 제25권4호
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• pp.202-208
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• 2015

Alumina dispersion strengthening copper(ADSC) alloy has great potential for use in many industrial applications such as contact supports, frictional break parts, electrode materials for lead wires, and spot welding with relatively high strength and good conductivity. In this study, we investigated the oxidation behavior of ADSC alloys. These alloys were fabricated in forms of plate and round type samples by surface oxidation reaction using Cu-0.8Al, Cu-0.4Al-0.4Ti, and Cu-0.6Al-0.4Ti(wt%) alloys. The alloys were oxidized at $980^{\circ}C$ for 1 h, 2 h, and 4 h in ambient atmosphere. The microstructure was observed with an optical microscope(OM) and a scanning electron microscope(SEM) equipped with energy-dispersive X-ray spectroscopy(EDS). Characterization of alumina was carried out using a 200 kV field-emission transmission electron microscope(TEM). As a result, various oxides including Ti were formed in the oxidation layer, in addition to ${\gamma}$-alumina. The thickness of the oxidation layer increased with Ti addition to the Cu-Al alloy and with the oxidation time. The corrected diffusion equation for the plate and round type samples showed different oxidation layer thickness under the same conditions. Diffusion length of the round type specimen had a value higher than that of its plate counterpart because the oxygen concentration per unit area of the round type specimen was higher than that of the plate type specimen at the same diffusion depth.

• Journal of Microbiology and Biotechnology
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• 제22권6호
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• pp.856-865
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• 2012

We describe the construction and immunobiological properties of a novel whooping cough vaccine candidate, in which the aroQ gene, encoding 3-dehydroquinase, was deleted by insertional inactivation using the kanamycin resistance gene cassette and allelic exchange using a Bordetella suicide vector. The aroQ B. pertussis mutant required supplementation of media to grow but failed to grow on an unsupplemented medium. The aroQ B. pertussis mutant was undetectable in the trachea and lungs of mice at days 6 and 12 post-infection, respectively. Antigen-specific antibody isotypes IgG1 and IgG2a, were produced, and cell-mediated immunity [CMI], using interleukin-2 and interferon-gamma as indirect indicators, was induced in mice vaccinated with the aroQ B. pertussis vaccine candidate, which were substantially enhanced upon second exposure to virulent B. pertussis. Interleukin-12 was also produced in the aroQ B. pertussis-vaccinated mice. On the other hand, neither IgG2a nor CMI-indicator cytokines were produced in DTaP-vaccinated mice, although the CMI-indicator cytokines became detectable post-challenge with virulent B. pertussis. Intranasal immunization with one dose of the aroQ B. pertussis mutant protected vaccinated mice against an intranasal challenge infection, with no pathogen being detected in the lungs of immunized mice by day 7 post-challenge. B. pertussis aroQ thus constitutes a safe, non-reverting, metabolite-deficient vaccine candidate that induces both humoral and cell-mediated immune responses with potential for use as a single-dose vaccine in adolescents and adults, in the first instance, with a view to disrupting the transmission cycle of whooping cough to infants and the community.

• Journal of Magnetics
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• 제15권4호
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• pp.179-184
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• 2010

We have investigated the effects of post annealing on iron oxide nanoparticles synthesized by the novel hydrothermal synthesis method with the $FeSO_4{\cdot}7H_2O$. To investigate the post annealing effect, the as-synthesized iron oxide nanoparticles were annealed at different temperatures in a vacuum chamber. The morphological, structural and magnetic properties of the iron oxide nanoparticles were investigated with high resolution X-ray powder diffraction (XRD), high resolution transmission electron microscopy (HRTEM), Mossbauer spectroscopy, and vibrating sample magnetometer analysis. According to the XRD and HRTEM analysis results, as-synthesized iron oxide nanoparticles were only magnetite ($Fe_3O_4$) phase with face-centered cubic structure but post annealed iron oxide nanoparticles at $700^{\circ}C$ were mainly magnetite phase with trivial maghemite ($\gamma-Fe_2O_3$) phase which was induced in the post annealing treatment. The crystallinity of the iron oxide nanoparticles is enhanced by the post annealing treatment. The particle size of the as-synthesized iron oxide nanoparticles was about 5 nm and the particle shape was almost spherical. But the particle size of the post annealed iron oxide nanoparticles at $700^{\circ}C$ was around 25 nm and the particle shape was spherical and irregular. The as-synthesized iron oxide nanoparticles showed superparamagnetic behavior, but post annealed iron oxide nanoparticles at $700^{\circ}C$ did not show superparamagnetic behavior due to the increase of particle size by post annealing treatment. The saturation of magnetization of the as-synthesized nanoparticles, post annealed nanoparticles at $500^{\circ}C$, and post annealed nanoparticles at $700^{\circ}C$ was found to be 3.7 emu/g, 6.1 emu/g, and 7.5 emu/g, respectively. The much smaller saturation magnetization value than one of bulk magnetite can be attributed to spin disorder and/or spin canting, spin pinning at the nanoparticle surface.

• 한국컴퓨터정보학회논문지
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• 제17권6호
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• pp.75-81
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• 2012

본 논문에서는 QoS 라우팅 알고리즘의 성능을 유지하면서 LSU(Link State Update) 메시지의 수를 제어할 수 있는 QoS 라우팅 링크 상태 갱신 알고리즘을 제안하였다. 기존에 제시된 대부분의 LSU 알고리즘은 QoS 라우팅의 성능을 향상시키는데 중점을 두고 있기 때문에 LSU 메시지의 수가 늘어나더라도 제어할 수 있는 메커니즘을 가지고 있지 않다. 특히 트래픽 통계에 근거한 적응형 알고리즘의 경우 더욱더 그러하며 트래픽이 과도하거나 변화가 심할 경우 이와 비례해서 LSU 메시지 수도 증가하여 과도한 LSU 메시지가 성능을 오히려 좋지 않게 한다. 제시하는 알고리즘은 QoS 라우팅 성능과 상충관계에 있는 과도한 LSU 메시지의 수를 제어하기 위해 요구 대역폭이 가용대역폭에 미치는 영향에 따라 LSU 메시지의 중요도를 구분하고 중요도와 단위시간 당 업데이트 비율 ${\gamma}$에 따라 LSU 메시지의 전송 여부를 결정하여 LSU 메시지 수를 제어한다. 성능 평가를 위해 기존에 제시된 다양한 LSU 알고리즘과 본 논문에서 제안하는 알고리즘을 MCI 네트워크상에서 라우팅 Blocking 확률과 링크 당 평균 LSU 메시지의 개수 등을 성능 평가 항목으로 하여 시뮬레이션을 수행하였고 제안하는 알고리즘의 우수성을 확인하였다.