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Effects of Osmotic and Non-Osmotic Priming Methods for Enhancing Germination of Pepper Seed

  • Kim, Min Geun;Kang, Won Sik;Kim, Du Hyun
    • Proceedings of the Plant Resources Society of Korea Conference
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    • 2018.10a
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    • pp.87-87
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    • 2018
  • This study was conducted to investigate the effects of osmotic priming, liquid smoke (LS), sonication and modified drum priming treatments to improve the germination speed and uniformity of pepper seeds for high quality export seeds. Seeds were treated in 0.5, 1.0, 5.0, 10% LS solution only or with 100mM $K_2SO_4$ solution for 6days at $15^{\circ}C$. Sonication treatment was performed for 5, 10, and 20 minutes at an intensity of 5.2, 10.4, 15.7, 21.0, 26.1kHz in water at $15^{\circ}C$. After sonication treatment, seeds were primed with water or 100mM $K_2SO_4$ for 4days. 40, 50 and 60% seed moisture content (SMC) of hydrated seeds were incubated for 84, 96 and 108h in a container with a relative humidity of 99% at 26rpm for a modified drum priming treatments. 0.5% LS treatment with water or 100mM $K_2SO_4$ priming showed significant effects with 71% GP (9.0days MGT) and 66% GP (7.4days MGT), respective, while untreated seeds resulted in only 61% GP and 10days MGT. The healthy seed % was increased by 24% by $K_2SO_4$ priming with LS treatment compared to untreated seeds. However, sonication treatment showed less germination or no difference compared to untreated seed, regardless of intensity and treatment time. The modified drum priming treatment (108h incubation after 50% SMC hydration) significantly improved the GP (on the $4^{th}$days after sowing), germination rate (GR), and MGT to 65%, $28%{\cdot}day^{-1}$, and 3.6days, whereas untreated seed resulted only 7% GP, $17%{\cdot}day^{-1}$ GR and 5.9days MGT. Thus, modified drum priming treatment effectively improved seed germination and is considered as an industrially promising treatment methods considering the shortening of the treatment period and environment-friendly aspects.

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Occurrence Ecology and Chemical Control of Soil-flakes in Flood-direct Seeded Rice Field (담수직파(湛水直播)논의 괴불발생(發生) 생태(生態) 및 방제(防除))

  • Park, J.E.;Park, T.S.;Ryu, G.H.;Lee, I.Y.;Lee, H.K.;Lee, J.O.;Kuramochi, H.
    • Korean Journal of Weed Science
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    • v.15 no.1
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    • pp.13-18
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    • 1995
  • This experiment was carried out to investigate occurrence ecology and control of soil-flakes in flood-direct seeded rice field. The occurrence of soil-flakes were inhibited in blue light of 450nm wave length and promoted in red light of 660nm wave length. The diatoms of Pinnularia spp. largely occurred in low fertilizer level and which much more increased when nitrogen was mixed with thinly soil depth. The diatoms of Pinnularia spp. were effectively controlled by carbamate and triazine herbicides when treated in pre-emergence of soil-flakes. Beside, triazine herbicides, simetryne, dimethametryn, prometryne 5g ai/10a showed high control effect in pre and post-emergence of soil-flakes. Expecially, In water-direct seeded rice pyrazosulfuron-methyl molinate simetryne 5.37% GR and dimepiperate bensulfuron dimethametryn 7.643% GR showed high control effect on the soil-flakes when treated at 10 days after seeding and didn't showed rice injury.

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Effect of Lime and Temperature on the Changes of Available Soil Nutrients in Acid Sulfate Soil under Submergence (특이산성토양(特異酸性土壤)에서 담수시(湛水時) 석회처리(石灰處理) 및 온도조절(溫度調節)이 토양(土壤)의 유효성분(有效成分) 변화(變化)에 미치는 영향(影響))

  • Kang, Ui-Gwm;Ha, Ho-Sung
    • Korean Journal of Soil Science and Fertilizer
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    • v.18 no.3
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    • pp.282-288
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    • 1985
  • This experiment was conducted to investigate the changing patterns of the available elements by the control of lime addition amount and temperature in Acid Sulfate Soils under the submerged condition. The results obtained were summarized as follows: 1. pH and contents of available phosphate, soluble silicate, $NH_4-N$, and exchangeable iron in soils were decreased but exchangeable aluminium and manganese, and water soluble sulfur in soils increased after submergence. 2. Lime treatment increased pH, available phosphate, soluble silicate, $NH_4-N$, and water soluble sulfur, but that decreased exchangeable aluminium, iron, and manganese in soils. 3. Treatment with 12me/100gr of Ca as $CaCO_3$, showed the marked effect in increasing the exchangeable aluminium and iron, and increasing pH value to about 6.5 as well as available phosphate and $NH_4-N$. 4. Increases in available phosphate, $NH_4-N$, and exchangeable iron with aging of the soil flooded and lime treated were higher at $35^{\circ}C$ than those at $25^{\circ}C$. 5. Throughout submerged period a significant positive correlation was observed between pH and soluble silicate while the pH has negative correlation with exchangeable elements such as aluminium, iron, and manganese etc.

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Photochemical Damage and Responses of Antioxidant Enzymes in Rice Leaves Induced to Light-Chilling (Light-chilling에 의해 유도된 벼 잎에서의 광합성 변화와 항산화 효소의 반응)

  • Koo, Jeung-Suk;Choo, Yeon-Sik;Lee, Chin-Bum
    • Journal of Life Science
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    • v.19 no.4
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    • pp.442-448
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    • 2009
  • We investigated photooxidation and responses of antioxidant enzymes involved in scavenging reactive oxygen species (ROS) after light-chilling ($4^{\circ}C$) for 2 days and post chilling ($25^{\circ}C$) in rice leaves. Chilling leaves indicated a 50% reduction in photosynthetic efficiency ($F_v/F_m$ ratio) and a 48% increase of $H_2O_2$, respectively, compared to the control group. In comparison with the control, activities of superoxide dismutase (SOD) and glutathione reductase (GR) increased at light-chilling and post-chilling. CuZn-SOD and Mn-SOD among SOD forms were detected in rice leaves, while Fe-SOD was not found. The increase of SOD and GR activity may serve as a basis for defense against chilling injury as it dismutase superoxide generated by light-chilling. Catalase (CAT) activity decreased during light-chilling, while activity of APX showed remarkable increase during light-chilling in rice leaves. Among CAT isoforms analyzed by 10% native PAGE, activities of isoform -2 and -3 were inhibited during light-chilling. From the elevated APX activity and decreased CAT activity, we suggest that these two enzymes show mutual supplementary relationships, indicating different tendency during light-chilling.

Study on Stable Fly Eradication by :Sterile-Male Technique. 1) Mass Rearing of the Stable Fly. Stemoxys calcitrans L. (웅성불임기술을 이용한 쇠파리 구제에 관한 연구 1) 쇠파리의 인공대량사육에 관하여)

  • Chung K. H.;Ryu J.;Kim Y. R.;Kwon S. H.;Park J. D.;Kang T. S.
    • Korean journal of applied entomology
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    • v.12 no.1
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    • pp.41-46
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    • 1973
  • 1) The optimum temperature for mass rearing of stable fly was $26^{\circ}C$ centigrade. Number of days required for stage of development at $26^{\circ}C$ were 6.8 days for larval stage, 5.3 days for pupla stage, 10.4 days for preovipositionla stage, and 30 days for adult stage respectively. 3) The pupation rate, emergence rate and sex ratio were $80.7\%,\;84.3\%$ and 1 : 1, respectively. 3) The average weight of pupae was 14.5mg, and the standard medium showed better result in larvae rearing than wheat bran medium. 4) The optimum number of eggs for inoculation on 125gr medium was approximately 310. 5) Optimum size of resting place was determined as $2inch^2/adult$ when it reared in a rectangular cage.

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Effects of Sonication, Osmotic Priming and Modified Drum Priming on the Germination of Tomato Seeds

  • Kim, Min Geun;Kang, Won Sik;Kim, Du Hyun
    • Proceedings of the Plant Resources Society of Korea Conference
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    • 2018.10a
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    • pp.88-88
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    • 2018
  • In order to increase the germination speed and uniformity of tomato seeds, sonication and modified drum priming treatments were investigated to produce high quality seeds for export. Sonication treatment was performed for 5, 10 and 20minutes at an intensity of 5.2, 10.4 and 15.7kHz in water at $15^{\circ}C$. After sonication treatment, seeds were primed with water or 100mM $KNO_3$ for 4days. 40, 50 and 60% seed moisture content (SMC) of hydrated seeds were incubated for 60, 72 and 84h in a container with a relative humidity of 99% at 26rpm for a modified drum priming treatments. Germination speed were highly improved by sonication with osmotic priming. The seed treatment of osmotic priming or hydro priming after sonication or sonication without priming enhanced germination percentage (GP) on the $2^{rd}$day after sowing to 46%, 43% and 28%, respectively, while untreated seeds resulted in only 1% GP. These treatments also highly improved mean germination time (MGT) to 1.4, 1.8 and 2.6days, respectively, when compared to 3.5days MGT of untreated seeds. The modified drum priming treatment (72h incubation after 60% SMC hydration) significantly improved results of 74% GP(on the $3^{rd}$day after sowing), 2.6days MGT and $39%{\cdot}day^{-1}$ germination rate (GR), however, untreated seeds showed 19% GP, 4.1 MGT, and $25%{\cdot}day^{-1}$ GR. Although osmotic priming after sonication, hydro priming showed similar improved germination characteristics, however, modified drum priming is considered as an industrially promising treatment methods considering the shortening of the treatment period and environment-friendly aspects.

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Electrocatalytic Oxidation of NADH at the Modified Graphite Electrode Incorporating Gold Nano Particles (금 나노입자를 회합시킨 수식된 흑연전극으로 NADH의 전기촉매 산화반응)

  • Cha, Seong-Keuck;Han, Sung-Yub
    • Journal of the Korean Electrochemical Society
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    • v.10 no.1
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    • pp.1-6
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    • 2007
  • Mercaptopropionic acid(mpa) has been used to make self-assembled monolayer(SAMs) on the surface of graphite electrode incorporating gold nano particles, which are subsequently modified with dopamine(dopa). Such modified electrodes haying types of Gr(Au)/mpa-dopa were employed in the electrocatalytic oxidation of NADH. The responses of such modified electrodes were studied in terms of electron transfer kinetics and reaction procedure in the reaction. The reaction of the surface immobilized dopa with NADH was studied using the rotating disk electrode technique and a value of $5.06{\times}10^5M^{-1}s^{-1}$ was obtained for the second-order rate constant in 0.1 M phosphate buffer(pH=7.0), which was a $EC_{cat}$ and kinetic controlled procedure. But, the modified electrodes were diffusion controlled reaction having $4.64{\times}10^{-4}cm^2s^{-1}$ of the coefficient within $10^{-3}s$ after starting the reaction.

Isolation and Characterization of Aniline-Degrading Bacteria

  • Kahng, Hyung-Yeel;Kim, Seung-Il;Woo, Mi-Jeong;Park, Yong-Keun;Lee, Yung-Nok
    • Korean Journal of Microbiology
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    • v.30 no.3
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    • pp.199-206
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    • 1992
  • Six isolated strains degrading aniline were selected, identified and designated as pseudomonas putida K6, Pseudomonas acidovorans K82, Achromobacter gr. D. V. K24, Achromobacter xylosocidans K4, Moraxella sp. K21 and Moraxella sp. K22. All of them degraded 1000 ppm aniline completely within 30 to 36 hours. Most of these strains are resistant to antibiotics more than one, but Moraxella sp. has not any antibiotic marker tested. Most strains except for P. acidovorans K82 were shown to have resistance to the heavy metal ions such as Ni, Cu, Li, Ba, Co, etc. but not to Hg to which only P. putida K6 was resistant. M. sp. K21 was capable of degrading aniline to a maximum concentration of 2500 ppm without any repression. The incubation of the cell in limited pH ranges (4-8) had no great effect on aniline degradation. The addition of bactopeptone to the minimal media promoted the speed of aniline degradation, but the addition of glucose rather repressed the rate of aniline degradation. Through enzyme assay, A. gr. D. V. K 24 was shown to degrade aniline through artho-pathway and formed .betha.-ketoadipate as intermediate metabolite.

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DNA Methylase and Chloroplast DNA Methylation in Chlamydomonas (Chlamydomonas에서 분리한 DNA Methylase와 엽록체 DNA Methylation)

  • 김남곤
    • Journal of Plant Biology
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    • v.35 no.4
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    • pp.415-423
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    • 1992
  • Chlamydomonas reinhardtii 21 gr(mt+) strain의 배우체로부터 두 종류의 DNA methylase를 부분 분리하여 몇가지 기질 DNA에 대한 효소 활성을 측정하였다. DNA methylase I과 II는 동일한 pH와 ionic strength에서 서로 상이한 물리적인 성질과 서로 다른 분자량을 가지며 DNA methylase I과 II는 모두가 DNA 염기 중 adenine보다는 cytosine에 methylation을 수행하는 것으로 생각된다. 합성 DNA를 사용한 실험에서 DNA methylase I과는 달리 DNA methylase II는 poly(dA-dC)·poly(dG-dT)에서 보다 poly(dG-dC)·poly(dG-dC)의 oligonucleotide에서 더 높은 효소활성을 나타내었다. Chlamydomonas reinhardtii에서 추출한 엽록체 DNA를 기질로 사용하였을 때 DNA methylase I과 II 모두가 배우체기 보다는 영양생장기의 엽록체 DNA에 더 높은 활성을 나타내었다.

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Behavior of New Heribicide Bensulfuron methyl (DPX-F5384) in Soil (토양중(土壤中)에 있어서 신규(新規) 제초제(除草劑) Bensulfuron methyl (DPX-F5384) 의 행동(行動))

  • Jang, I.S.;Moon, Y.H.;Ryang, H.S.
    • Korean Journal of Weed Science
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    • v.7 no.1
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    • pp.74-77
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    • 1987
  • This study was undertaken to elucidate the behavior of herbicide bensulfuron methyl [ methyl 2-[[[[[(4, 6-dimethoxy pryrimidine-2yl) amino] carbonyl] amino] sulfonyl] methyl] benzoate] in soils under flooded conditions using the test plant Monochoria vaginalis Pres 1. Besulfuron methyl moved to 3cm depth in clay loam soil and 4 cm depth in sandy loam soil. Herbicide-treated layer was found 0 to 2 cm profile in the former and 0 to 3 cm profile the latter. The half life (GR50) was 87 days in clay loam soil and 78 days in sandy clay loam soil. The period of inactivation lasted for 110 days in clay loam and 100 days in sandy clay loam soil.

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