• Journal of the Korean Society of Tobacco Science
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• v.2 no.2
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• pp.20-37
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• 1980

Among the 34 strains of Nicotinophiles selected in the previous experiments, strain NCT27 identified with Pseudomonas putida and strain NCT30 identified with Arthrobacter oxydans biotype nan thus were Investigated for optimization of growth conditions for nicotine degradation and other cultural characteristics. The compositions of optimized medium were to be following: $KH_2PO_4$ 2.Ogr, KCI 5.Ogr, $MgSO_4$.$7H_2O$ 20mg, $MnSO_4$.$6H_2O$ 0.2mg, $FeSO_4$.$7H_2O$ 1.Omg, Col$^{++}$ (Cobalt Acetate),2.O$\gamma$, N1$^{++}$ (NiSO4,6H2O) 0.5$\gamma$, and yeast extract 80mg per liter. The optimum initial concentrations of nicotine for growth were 0.4% for Pseudomonas and 0.1% for Arthrobacter, respectively. The optimum temperature and pH were 3$0^{\circ}C$ and 7.0 for both of strains. The pH of culture medium of Pseudomonas was changed from acidic condition to basic one in going from the logarithmic growth phase to the stationary growth phase. In contrast with Pseudomonas, it remained constant in case of Arthrobacter. The growth of Arthrobacter was completely inhibited in the nicotine concentration of 0.7&. However, Pseudomonas could grow even in the nicotine concentration of 1.0%. Moreover, it could grow successfully in the tobacco extract media as well as media containing carbon and nitrogen sources other than nicotine. The maximum rates of nicotine degradation were to be 1.22 gr./hr./liter for Pseudomonas and 0.186 gr./hr./liter for Arthrobacter, respectively.

• The Journal of Pediatrics of Korean Medicine
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• v.30 no.4
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• pp.29-59
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• 2016

Objectives PRAL (Prunus armeniaca Linne Var) has been known to suppress allergic reaction. However, the cellular target and its mechanism of action were unclear. This study was designed to investigate the effect of PRAL on RBL-2H3 mast cell, which is PMA-Ionomycin-induced activated in vitro and the effect of PRAL on the MNC/Nga mice that are DNCB-induced activated in vivo. Methods In this study, IL-4, IL-13 production were examined by ELISA analysis; IL-4, IL-13, IL-31, IL-31Ra, $TNF-{\alpha}$ and GM-CSF mRNA expression were examined by Real-time PCR; manifestations of AP-1 and MAPKs transcription factors were examined by western blotting in vitro. Then skin rashes have been evaluated and verified the distribution of mast cells by H&E and toluidine blue. Also, WBC, eosinophil and neutrophil, IgE level in serum, $IFN-{\gamma}$, IL-4, IL-5 in the splenocyte culture supernatant, the absolute cell numbers of $CD4^+$, $CD8^+$, $Gr-1^+CD11b^+$, $B220^+CD23^+$, $CD3^+CD69^+$ in the Axillary Lymph Node (ALN), PBMCs and dorsal skin and IL-5, IL-13, IL-31, IL-31Ra in the dorsal skin by Real-time PCR were all evaluated from the NC/BNga mice. Results As a result of this study, the mRNA expression of IL-4, IL-13, IL-31, IL-31Ra and $TNF-{\alpha}$ and IL-4, IL-13 production, shown in ELISA analysis, were suppressed by PRAL. Results from the western blot analysis showed decrease on the expression of mast-cell-specific transcription factors, including AP-1 and p-JNK, p-ERK. Histological examination showed that infiltration levels of immune cells in the skin of the AD-induced NC/Nga mice were improved by PRAL orally adminstration. Orally- administered PRAL group also showred decreased level of IgE in the serum. This group has shown decreased the level of IL-4, IL-5, but shown elevated $IFN-{\gamma}$ level in the splenocyte culture supernatant. The same group also has shown decreased cell numbers of $CD4^+$, $CD8^+$, $CD3^+CD69^+$ in the ALN, and $CD4^+$, $Gr-1^+CD11b^+$ in the dorsal skin. PRAL oral adminstration increased cell numbers of $CD4^+$, but decreased cell numbers of $CD8^+$, $Gr-1^+CD11b^+$, $B220^+CD23^+$ in the PBMCs. Conclusions Obtained results suggest that PRAL can regulate molecular mediators and immune cells that are functionally associated with atopic dermatitis (AD) induced in the NC/Nga mice. This may play an important role in recovering AD symptoms and suppressing pruritus.

• Journal of Marine Life Science
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• v.3 no.2
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• pp.74-80
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• 2018

Mercury (Hg) poses a threat to marine ecosystem due to continuous inflow from various industries and bioaccumulation to higher trophic level via food web. Mercury can adversely affect growth, development, reproduction and metabolism to aquatic organisms. In the present study, acute toxicity and oxidative stress markers (total glutathione content, and activities of GST, GR and GPx) were investigated in brackish water flea Disphanosoma celebensis exposed to HgCl₂ for 24 h. As results, Hg showed negative effect in survival of D. celebensis. 24 h-LC₅₀ value was determined as 0.589 mg/l (95% C.I. 0.521~0.655 mg/l). After exposure to Hg (0.08 and 0.4 mg/l) for 24 h, total glutathione content was significantly decreased, whereas GST, GPx and GR activities were enhanced. These findings indicate that Hg induced oxidative stress in D. celebensis, and oxidative stress markers may be involved in cellular defense against Hg - mediated toxicity. This study provides a better understanding of molecular mode of action of Hg toxicity in this specie and potent of molecular markers for heavy metal monitoring in marine ecosystem.

• Journal of Electrochemical Science and Technology
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• v.12 no.2
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• pp.173-187
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• 2021

In the present research, a simple electrochemical sensor based on a carbon paste electrode (CPE) modified with ZnO-Zn₂SnO₄-SnO₂ and graphene (ZnO-Zn₂SnO₄-SnO₂/Gr/CPE) was developed for the direct, simultaneous and individual electrochemical measurement of Acetaminophen (AC), Caffeine (Caf) and Ascorbic acid (AA). The synthesized nano-materials were investigated using scanning electron microscopy, X-ray Diffraction, Fourier-transform infrared spectroscopy, and electrochemical impedance spectroscopy techniques. Cyclic voltammetry and differential pulse voltammetry were applied for electrochemical investigation ZnO-Zn₂SnO₄-SnO₂/Gr/CPE, and the impact of scan rate and the concentration of H⁺ on the electrode's responses were investigated. The voltammograms showed a linear relationship between the response of the electrode for individual oxidation of AA, AC and, Caf in the range of 0.021-120, 0.018-85.3, and 0.02-97.51 μM with the detection limit of 8.94, 6.66 and 7.09 nM (S/N = 3), respectively. Also, the amperometric technique was applied for the measuring of the target molecules in the range of 0.013-16, 0.008-12 and, 0.01-14 μM for AA, AC and, Caf with the detection limit of 6.28, 3.64 and 3.85 nM, respectively. Besides, the ZnO-Zn₂SnO₄-SnO₂/Gr/CPE shows an excellent selectivity, stability, repeatability, and reproducibility for the determination of AA, AC and, Caf. Finally, the proposed sensor was successfully used to show the amount of AA, AC and, Caf in urine, blood serum samples with recoveries ranging between 95.8% and 104.06%.

• The Korean Journal of Ecology
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• v.26 no.5
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• pp.273-280
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• 2003

Saline conditions invoke oxidative stress attributed to the overproduction of reactive oxygen species (ROS). Changes in quantum efficiency and antioxidative enzyme activity upon salt treatment were examined in a salt-tolerant plant, Atriplex gmelini, to test the hypothesis that salt tolerance of A. gmelini is due to the increased activity of antioxidative enzymes. A. gmelini showed optimum growth at 100 mM NaCl producing 116% of the shoot dry weight over control plants in 0 mM NaCl treatment. Healthy growth persisted up to 300 mM NaCl treatment maintaining normal internal water content and dry weight. No photochemical stress or damages on antioxidative defense system was obvious in plants of 2 and 4 day salt treatment which was indicated by increased quantum efficiency (Fv/Fm value), decreased stress index (Fo/Fm value), and increased activity of antioxidative enzymes such as SOD, APX, GR. However, the plants treated with 400 mM NaCl showed decrease in growth and in antioxidative enzyme activity although the enzyme activity was still higher than that of the 0 mM NaCl treated plants (l31%, 114%, and 134% of the SOD, APX, and GR activity, respectively). Interestingly, another important antioridative enzyme that scavenges H₂O₂ in plant cells, CAT, showed rapid decrease in its activity as salt concentration increased; 38%, 22%, 15% of the 0 mM NaCl treated plants at 200, 300, 400 mM NaCl treatments, respectively. It appears that the enzymes in ascorbate-glutathione cycle such as APX and GR play the major roles in scavenging ROS produced by salt stress in A. gmelini. After 6 days of salt treatment, the damage in photochemical and antioxidative defense system was indicated by decreased Fv/Fm value and increased Fo/Fm value. A. gmelini appears to cope with short term salt treatment by enhanced activity of the antioxidative defense system, whereas long term stress invoke oxidative stress by increased ROS due to the damages in photochemical and antioxidative system.

• Journal of Advanced Marine Engineering and Technology
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• v.22 no.3
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• pp.294-302
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• 1998

The charateristics of the convective mass transfer in horizontal rectangular enclosure with horizontal concentration gradients are analyzed. The effect of Grashof number(Gr) and aspect ratio(L/H) is investigated numerically using the control-volume method. Numerical results are obtained for Grashof numbers between $10^4$ and $10^6$ aspect ratios from 1 to 100 and results are compared with existing andlytical results. It is found that there exists a well defined aspect ratio for which the mean Sherwood number is maximum and the core flow changes from parallel to non-parallel at $Gr^2{Sc^2}(A^{-3}}{\geq}{10^5}$ and in the Ralongrightarrow0 regime the numerical results agreed very well with correlation derived from analytical results.

• Korean Journal of Soil Science and Fertilizer
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• v.6 no.1
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• pp.1-8
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• 1973

This study examined the morphological, physical and chemical characteristics of the Yesan and the Songjeong series derived from residuum of the granite developed on gently sloping to rolling relief in Honam reclamable land Area. The results of this study are summarized as follows. 1. The morphological characteristics. In the Yesan series, the surface soils(Ap horizons) are brown to dark brown sandy loam. The subsoils(B horizons) are yellowish red to red sandy clay loam to sandy loam and the soil profile development is weak. The Songjeong series, the surface soils (Ap horizons) are washed by erosion, so the subsoils are revealed on the surface, and these are dark red silty clay loam. The subsoils (B horizons) are red silty clay loam and thin clay cutans are formed on the ped faces of the structure. The substrata of two soil series are deeply weathered granitic saprolite. 2. The physical and chemical characteristics. The distribution of clay content tends to increase from surfaces to subsoils with depth gradually. On the Yesan series, the content of clay is less than 18%, soil pH (6.0 in the surface-soil, 4.5-5.0 in the subsoil), the content of organic matter (1.8% in the surface soil, 0.1~0.4% in the subsoil), available phosphate (40 ppm), the cation exchange capacity(4~8 me/100 gr) are very low, and the base saturation (57.8% in the surface soil, 46.3% in the subsoil) is moderate. On the Songjeong series, the content of clay is 30~40%, pH (5.7-6.0), the content of in organic matter (1.25% in the surface soil, 0.1~0.4% in the subsoil), available phosphate(4 ppm), the cation exchange capacity(6.2 me/100 gr in the surface soil, 2~6 me/100gr in the subsoil) are very low, and the base saturation(28.1% in the surfacesoil, 16~23% in the subsoil) is also low. 3. The Yesan and Songjeong series are for med under a temperate humid climate, and classified as Red Yellow Soils in the old classification system. According to U.S.D.A. 7th approximation the former belongs to Typic Dystrochrepts in Inceptisols, and the latter, Typic Hapludults in Ultisols.

• Journal of The Korean Society of Grassland and Forage Science
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• v.5 no.2
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• pp.93-99
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• 1985

All soil characteristics for 10,077ha of establishable grassland in 1984 were statistically summarized. Soils could be classified into 5 of Order, 7 Suborder, 11 Great group, 28 Subgroup, and 105 Series by the new comprehensive classification system. Distribution rate of farm houses managed the extent magnitude of 3.0ha was 70.8% and its total extent was 35.9%. Extent rate of uncultivated method was 71.3%. These soils were distributed with regard to hilly land and mountain foot slope at, 82.0, and to 0-30% slope at 66.9%. Soil texture was light such as sandy loam to loam at 85.0%, available soil depth more than 20cm at 97.8%, and gravel content less than 35% at 98.4%. Soil would be distributed in 67.3 of first class, 29.7 second class, and 3.0% third class for suitability. Mean values of pH, available $P_2O_5$, OM, Ca, Mg and K for topsoil were 5.6, 31ppm, 3.2%, 2.23me/100gr, 1.05me/100gr, and 0.37me/100gr, respectively.

• Journal of the Mineralogical Society of Korea
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• v.16 no.1
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• pp.107-123
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• 2003

The objective of this study was to investigate mineral precipitates, which derived from the zero valent iron (ZVI) corrosion during TCE dechlorination and to find the controlling factors in mineral precipitates. A series of column experiemnts were conducted to evaluate the location of ZVI and the effects of electrode arrangements in electro-enhanced permeable reactive barrier (E2PRB) systems. Based on mineralogical study, ZVI samples near the influent port had more lepidocrocite, ferrihydrite or Fe (oxy)hydroxide, and (phospho)siderite while backward samples had more akaganeite, magnetite/maghemite, and intermediate green rust (GR) I and GR II. A suite of mineral distribution was preferabley related to the dissolved oxygen and the increased pH. Controlling factors of mineral precipitates in an E2PRB system were found to be (1) pH, (2) dissolved oxygen, (3) the types of Fe intermediates, and (4) anionic species to form complex strongly.

• Journal of the Korean Society of Food Science and Nutrition
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• v.36 no.6
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• pp.759-765
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• 2007

This study was conducted to analyze the chemical properties of single.harvested pepper (YW211, YW213), and to investigate the change of kochujang quality by the addition of single-harvested peppers. Capsaicinoids content of YW211 was $271.65{\pm}25.10mg/100g$ and ASTA color value of YW213 was $212.71{\pm}2.38$, which were comparatively higher values than commercial red peppers. Mixed red pepper powder used for kochujang production were prepared with various mixing ratios of commercial red peppers (YY & GR) and single-harvested pepper (YW211 & YW213). Capsaicinoids content of YY, $3.98{\pm}0.24mg/100g$, was increased to $52.61{\pm}8.62mg/100g$ by mixing 30% YW211. ASTA color value of GR was $110.63{\pm}1.89$ and was increased to $130.01{\pm}1.31$ by mixing 30% YW213. The pH values of kochujang added YW211 or YW213 were slowly reduced and acidities were increased during fermentation; however, both values weren't statistically different. The contents of amino nitrogen increased until 60 days, and then decreased thereafter. Reducing sugars were increased considerably until 30 days, and reduced slowly after fermentation for 90 days.