• Title/Summary/Keyword: GPx

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Comparative Analysis on Antioxidant Activity in Various Human Skin Fibroblasts and Mesenchymal Stem Cells (사람의 피부 섬유아세포 및 중간엽 줄기세포에서 항산화 활성의 반응에 대한 비교 분석)

  • Kong, Ji-Weon;Park, Ryeok;Park, Joon-Woo;Lee, Joo-Yeong;Choi, Yeon-Joo;Moon, Sun-Ha;Kim, Hyeon-Ji;Jeon, Byeong-Gyun
    • Journal of the Korean Applied Science and Technology
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    • v.36 no.2
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    • pp.394-406
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    • 2019
  • The cellular senescence may be due to damage by the reactive oxygen species (ROS). This study has compared the antioxidant activity in the human cell lines of various origins, including 10S and 50S-derived normal skin fibroblasts, and 10S bone marrow, dental tissue and adipose-derived adult stem cells. After being exposed to $H_2O_2$, half inhibitory concentration ($IC_{50}$) values by cytotoxicity assay was significantly (P<0.05) lower in 50S-derived skin fibroblasts, than in 10S-derived skin fibroblasts and various adult stem cell lines. The cell population doubling time (PDT) and the cell frequency with high senescence associated-${\beta}$-galactose activity were remarkably increased in 50S-derived fibroblasts exposed to 50 ppm $H_2O_2$ for 7 days, than those of 10S-derived fibroblasts and various adult stem cell lines. Further, the expression level of antioxidant-related genes, glutathione peroxidase (GPX) and catalase (CAT), was investigated in 10S and 50S-derived skin fibroblasts, and 10S-derived various adult stem cells by reverse transcription polymerase chain reaction (RT-PCR). The expression level of GPX was higher in most of cell lines, compared to CAT, and a significantly (P<0.05) higher expression level of GPX was observed in 10S-derived skin fibroblasts and adult stem cell lines, compared to 50S-derived skin fibroblasts. We concluded that old-aged skin fibroblasts seemed to be less resistant against ROS than young-aged skin fibroblasts and adult stem cells.

A Study for the Relation Between Selenoproteins and Korean Rectal Cancer Using Deuterium Collision Gas HPLC-ICP/MS (HPLC와 중수소 충돌기체 ICP/MS를 이용하여 분석한 셀레노단백질과 한국인 직장암과의 상관관계 연구)

  • Lee, Seo Young;Pak, Yong-Nam
    • Journal of the Korean Chemical Society
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    • v.63 no.6
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    • pp.420-426
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    • 2019
  • Selenoproteins,in Korean blood serum, GPx, SelP, and SeAlb were separated and determined with the use of HPLC-ICP/MS. Deuterium was used as a collision gas and affinity column with ammonium formate was used as an eluting solvent for the accurate quantitation of selenoproteins in human blood serum. Certified reference material BCR 639 (133±12 ng g-1) was tested for the accuracy and the result was satisfactory 130±6 ng g-1. Blood serum for the rectal cancer and controlled groups were collected and analyzed to give 84±27 ng g-1, and 119±28 ng g-1, respectively. The difference was statistically obvious when t-test was performed (tcal 4.93 > t95% 2.04). The decrease for cancer group was more obvious for female and aged group. The distributions of three selenoproteins were similar with each other, which means rectal cancer group did not show any specificity for any selenoproteins. As cancer developed, GPx showed a slight decrease but not obvious while the total concentration was increasing particularly at the second stage of cancer.

The Effects of Solanum nigum Linne extract on the Hepatotoxicity of Rats Induced by Lipopolysaccharide (Lipopolysaccharide로 유발된 간독성에 대한 까마중의 효과)

  • Heo, Ye-Young;Kwon, Ryun-Hee;Ha, Mi-Sook;Ha, Bae-Jin
    • Journal of Food Hygiene and Safety
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    • v.24 no.3
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    • pp.285-290
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    • 2009
  • This study aimed to investigate the protective effect of Solanum nigum Linne total extract (SNT), Solanum nigum Linne leaf extract (SNL), Solanum nigum Linne root extract (SNR) on liver injury induced by Lipopolysaccharide(LPS) in Sprague-Dawley rats. SNT, SNL, SNR of 100 mg/kg concentration was intraperitoneally administered into rats at dose of 1.5 ml/kg for 20 days. on the day 1.5 ml/kg of LPS was injected. Four hours later, they were anesthetization with ether and dissected. Glutamate oxaloacetate transaminase (GOT), glutamate pyruvate transaminase (GPT) were measured in serum and superoxide dismutase (SOD), catalase and glutathione peroxidase (GPX) were measured in liver homogenate. SNT, SNL, SNR extract inhibited GOT and GPT activities in LPS-induced rats, whereas increased SOD, Catalase and GPX activity in liver tissue of LPS-induced rats. These suggested that SNT, SNL, SNR could be used for functional beverage.

Effect of Sea Buckthorn Leaves on Hepatic Enzyme Levels in Streptozotocin Induced Diabetic Rats (비타민나무잎 식이보충과 당뇨흰쥐 간장의 항산화효소 수준에 미치는 영향)

  • Kim, Myung-Wha
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.42 no.1
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    • pp.40-45
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    • 2013
  • This study was designed to examine the effect of sea buckthorn (SBT) leaves on hepatic antioxidative enzyme levels in diabetic rats. Diabetes mellitus was induced in male Sprague-Dawley rats by an injection of streptozotocin (STZ). Sprague-Dawley rats were then fed for four weeks, with experimental groups receiving a modified diet containing 10% or 20% powder derived from SBT leaves. The experimental groups were divided into six groups: a normal (N)-control group, N-SBT 10% and N-SBT 20% treated groups, STZ-control, STZ-SBT 10% and STZ-SBT 20% treated groups. Malondialdehyde (MDA), superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPX), glutathione reductase (GR), glutathione-S-transferase (GST) and xanthine oxidase (XOD) levels were measured in liver cytosol. The results showed that the level of SOD was significantly increased in the N-SBT 20% group but not statistically different in the diabetic group. The level of CAT was significantly higher in the N-SBT 20% group compared to the control group. The level of GPX was significantly increased in the N-SBT 20% group and the diabetic supplementary group. In contrast, the level of XOD was significantly decreased in the diabetic group supplemented with SBT leaves.

The Preventive Effects of Paeoniae Radix Extract against LPS-induced Acute Hepatotoxicity (LPS로 유도된 급성 간독성에 대한 백작약 추출물의 보호 효과)

  • Kim, In-Deok;Kwon, Ryun-Hee;Heo, Ye-Young;Lee, Dong-Geun;Lee, Jae-Hwa;Lee, Sang-Hyeon;Ha, Jong-Myung;Ha, Bae-Jin
    • Journal of Food Hygiene and Safety
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    • v.23 no.3
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    • pp.222-226
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    • 2008
  • The purpose of this study was to investigate the preventive effects of Paeoniae Radix Extract(PRE) against the acute hepatotoxicity-inducing lipopolysaccharide(LPS) in the liver. PRE of 100 mg/kg concentration was intraperitoneally administered into rats at dose of 1.5 ml/kg for 20 days. On day 21, 5 mg/kg of LPS dissolved in saline was injected 4 hours before anesthetization. We examined the levels of glutamate oxaloacetate transaminase(GOT), glutamate pyruvate transaminase(GPT), lactate dehydrogenase(LDH) in serum of rats, superoxide dismutase(SOD) in mitochondrial fractions, and malondialdehyde(MDA), catalase(CAT), glutathione peroxidase(GPx) in liver homogenates. LPS-treatment markedly increased the levels of GOT, GPT, LDH and MDA, and significantly decreased those of SOD, CAT and GPx. But PRE-pretreatment decreased the levels of GOT, GPT, LDH and MDA, by 59.7%, 43.6%, 59.6% and 63.5%, respectively and increased those of SOD, CAT and GPx, by 85.5%, 57.8% and 62.9%, respectively. These results showed that the PRE had the preventive effects against the acute hepatotoxicity-inducing LPS in the liver.

Protective Effect of White-Skinned Sweet Potato (Ipomoea batatas L.) against Renal Damage in Streptozotocin-Induced Diabetic Rats (Streptozotocin으로 유발된 당뇨쥐의 신장 손상에 대한 white-skinned sweet potato (Ipomoea batatas L.) 추출물의 보호효과)

  • Jang, Hye-Won;Bachri, Moch. Saiful;Moon, Kyung-Ok;Park, Jong-Ok
    • Journal of Life Science
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    • v.20 no.2
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    • pp.161-168
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    • 2010
  • White-skinned sweet potato (Ipomoea batatas L.) has been traditionally used for diabetes treatment and management in many countries. In this experiment, methanol extract of white-skinned sweet potato (WSPMe) at a dose of 100 or 200 mg/kg body weight was tested to evaluate its effect on renal damage in streptozotocin (STZ)-induced diabetic rats. Its efficacy was compared with that of insulin secretogogue, glimepiride ($50\;{\mu}g/kg$ body weight). Experimental diabetes was induced by a single dose of STZ (45 mg/kg, i.p.) injection. The WSPMe and glimepiride were administered orally for 14 days and the effects on glucose, renal markers including blood urea nitrogen (BUN), creatinine and lactate dehydrogenase (LDH), lipid peroxide (LPO) level, antioxidant enzymes superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx) and glutathion-S-transferase (GST) activities in kidney were studied. An increase in BUN, creatinine, LDH, glucose, LPO levels and decrease in SOD, CAT, GPx and GST features were observed in diabetic control rats. Administration of WSPMe at a dose of 200 mg/kg body weight caused a significant improvement in blood glucose, LPO level, renal markers, lipid peroxidation markers and increased antioxidant levels in diabetic kidney. In conclusion, the WSPMe was found to be effective in reducing oxidative stress, thus confirming the ethnopharmacological use of I. batatas L. in protecting diabetes and its complications.

Ulva lactuca Fucoidan Extract and its Protective Effects on $CCI_4$-induced Liver Dysfunction ($CCI_4$로 유도된 간 기능장애에 대한 갈파래 푸코이단 추출물의 보호효과)

  • Nam, Chun-Suk;Kang, Kum-Suk;Ha, Bae-Jin
    • KSBB Journal
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    • v.22 no.2
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    • pp.73-77
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    • 2007
  • The effects of Fucoidan extracted from Ulva lactuca on carbon tetrachloride ($CCI_4$)-induced dysfunction in $CCI_4$-posttreated rats were investigated. Ulva lactuca fucoidan (ULF) of 100 mg/kg concentration was intraperitoneally administered into rats at dose of 1 ml/kg for 14 days. On the day 15, 3.3 ml/kg of $CCI_4$ dissolved in olive oil (1 : 1) was injected 12 hours before anesthetization. We examined the levels of glutamate oxaloacetate transaminase (GOT), glutamate pyruvate transaminase (GPT) in serum of rats, superoxide dismutase (SOD) in mitochondrial fraction and catalase (CAT), glutathione peroxidase (GPx), malondialdehyde (MDA) in liver of rats. SOD, CAT, GPx decreased, and GOT, GPT, MDA increased in the $CCI_4$-treated group. But SOD, CAT, GPx increased, and GOP, GPT, MDA decreased in the ULF and $CCI_4$-treated group. These results showed that ULF had the protective effects on the liver dysfunction of $CCI_4$-treated rats.

Hypolipidemic Effects of Glycoprotein Isolated from Ficus Carica Linnoeus in Mice (무화과 당단백질의 혈중지질 저하 효과)

  • Lim, Kye-Taek;Lee, Sei-Jung;Ko, Jeong-Hyeon;Oh, Phil-Sun
    • Korean Journal of Food Science and Technology
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    • v.37 no.4
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    • pp.624-630
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    • 2005
  • Glycoprotein (60 kDa) isolated from Ficus Carica Linnoeus (FCL glycoprotein) was examined by evaluating its hypolipidemic effects on plasma cholesterol levels and hepatic detoxicant enzyme activities in ICR mice. FCL glycoprotein $(100{\mu}g/mL)$ had strong scavenging activities (38%) against lipid peroxyl radicals. When mice were treated with Triton WR-1339 (400 mg/kg), levels of total cholesterol (TC) and low-density lipoprotein (LDL)-cholesterol in plasma significantly increased by 53.9 and 47.5 mg/dL, respectively, compared to the control, whereas, when pretreated with FCL glycoprotein $(100{\mu}g/mL)$, decreased remarkably by 55.4, and 47,0 mg/dL, compared to Triton WR-1339 treatment alone. Interestingly, high-density lipoprotein (HDL)-cholesterol level did not change. Body and liver weights did not change significantly after Triton WR-1339 treatment in presence of FCL glycoprotein. FCL glycoprotein $(100{\mu}g/mL)$ stimulated activities of antioxidative detoxicant enzymes such as superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GPx), whereas GPx activity significantly increased compared to the control. These results suggest FCL glycoprotein has abilities to scavenge lipid peroxyl radicals, lower plasma lipid levels, and stimulate detoxicant enzyme activity in mouse liver.

Antioxidative Activity of Pine (Pinus densiflora) Needle Extracts in Rats Fed High-Cholesterol Diet (고콜레스테롤 식이 섭취 흰쥐에 대한 적송잎 추출물의 항산화 효과)

  • Kang, Sung-Rim;Shin, Mee-Ok;Kim, Sung-Gu;Lee, Sang-Hyeon;Kim, Mi-Hyang
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.38 no.4
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    • pp.423-429
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    • 2009
  • This study was conducted to investigate the effects of pine (Pinus densiflora) needle extracts on hepatic antioxidative system in rats fed high-cholesterol diet. Eight-week old Sprague Dawley rats were divided into four groups; normal diet group (CON), 0.5% cholesterol diet group (0.5% CHOL), 0.5% cholesterol diet and pine needle extracts prepared by using hot water group (HOT water), and 0.5% cholesterol diet and pine needle extracts prepared by using sub-supercritical $CO_2$ group (Sub-supercritical). The serum ALP, AST, and ALT activities were increased by 0.5% cholesterol diet supplementation, but treatment with pine needle extracts showed decrease compared with 0.5% CHOL group. The SOD activity in liver at 0.5% cholesterol diet group was more decreased than control group while Sub-supercritical group had significantly higher SOD activity than 0.5% CHOL group. Moreover, groups with pine needle extracts supplementation had higher level of GPx in liver than 0.5% cholesterol diet group. These results suggest that pine needle extracts increased antioxidative activities.

Physiological Responses of Cultured Red Seabream Pagrus major and Olive Flounder Paralichthys olivaceus During Exposure to the Red Tide Dinoflagellate Cochlodinium polykrikoides (적조 Cochlodinium polykrikoides 노출에 따른 양식산 참돔과 넙치의 생리학적 반응 )

  • Hyo-Won, Kim;Hyun Woo, Gil;Young Jae, Choi;Yun Kyung, Shin
    • Journal of Marine Life Science
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    • v.7 no.2
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    • pp.171-179
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    • 2022
  • The present study investigated the survival rate, respiration rate, plasma stress index, and histological changes according to exposure time of cultured red seabream (Pagrus major) and olive flounder (Paralichthys olivaceus) exposed to Cochlodinium polykrikoides red tide. Fish cultured in natural seawater were used as the control group. Cochlodinium polykrikoides density was set to 5,500±200 cells·ml-1 in the experimental groups. All red seabreams died within 1 hour of exposure to red tide, whereas all olive flounders died within 5 hours of exposure. Analysis of physiological response revealed that in red seabream, plasma glutamic oxaloacetic transaminase (GOT), glutamic pyruvic transaminase (GPT), catalase (CAT), and glutathione peroxidase (GPx) concentrations were increased; plasma glucose and superoxide dismutase (SOD) concentration were decreased. Meanwhile, in olive flounders, plasma cortisol, GOT, and GPT concentrations were increased; plasma glucose concentrations were increased during the first hour of exposure, followed by decrease after 5 hours; and plasma SOD, CAT, and GPx concentrations decreased during the first hour of exposure. Histological analysis revealed structural damage to the gills of both red seabream and olive flounder. In conclusion, the exposure of red seabream and olive flounder to Cochlodinium polykrikoides red tide at the density of 5,500 cells·ml-1 induces oxidative stress, which activates antioxidant defense mechanisms, ultimately leading to liver and gill damage.