Browse > Article

The Preventive Effects of Paeoniae Radix Extract against LPS-induced Acute Hepatotoxicity  

Kim, In-Deok (Department of Pharmaceutical Engineering, College of Medical Life Science, Silla University)
Kwon, Ryun-Hee (Department of Pharmaceutical Engineering, College of Medical Life Science, Silla University)
Heo, Ye-Young (Department of Pharmaceutical Engineering, College of Medical Life Science, Silla University)
Lee, Dong-Geun (Department of Pharmaceutical Engineering, College of Medical Life Science, Silla University)
Lee, Jae-Hwa (Department of Pharmaceutical Engineering, College of Medical Life Science, Silla University)
Lee, Sang-Hyeon (Department of Pharmaceutical Engineering, College of Medical Life Science, Silla University)
Ha, Jong-Myung (Department of Pharmaceutical Engineering, College of Medical Life Science, Silla University)
Ha, Bae-Jin (Department of Pharmaceutical Engineering, College of Medical Life Science, Silla University)
Publication Information
Journal of Food Hygiene and Safety / v.23, no.3, 2008 , pp. 222-226 More about this Journal
Abstract
The purpose of this study was to investigate the preventive effects of Paeoniae Radix Extract(PRE) against the acute hepatotoxicity-inducing lipopolysaccharide(LPS) in the liver. PRE of 100 mg/kg concentration was intraperitoneally administered into rats at dose of 1.5 ml/kg for 20 days. On day 21, 5 mg/kg of LPS dissolved in saline was injected 4 hours before anesthetization. We examined the levels of glutamate oxaloacetate transaminase(GOT), glutamate pyruvate transaminase(GPT), lactate dehydrogenase(LDH) in serum of rats, superoxide dismutase(SOD) in mitochondrial fractions, and malondialdehyde(MDA), catalase(CAT), glutathione peroxidase(GPx) in liver homogenates. LPS-treatment markedly increased the levels of GOT, GPT, LDH and MDA, and significantly decreased those of SOD, CAT and GPx. But PRE-pretreatment decreased the levels of GOT, GPT, LDH and MDA, by 59.7%, 43.6%, 59.6% and 63.5%, respectively and increased those of SOD, CAT and GPx, by 85.5%, 57.8% and 62.9%, respectively. These results showed that the PRE had the preventive effects against the acute hepatotoxicity-inducing LPS in the liver.
Keywords
Paeonia lactiflora Pallas; LPS; anti-oxidation; hepatotoxicity;
Citations & Related Records
Times Cited By KSCI : 4  (Citation Analysis)
연도 인용수 순위
1 Goto, H., Shimada, Y., Akechi, Y., Kohta, K., Hattori, M. and Terasawa, K.: Endothelium-dependent vasodilator effect of extract prepared from the root of Paeonia lactiflora on isolated rat aorta. Planta. Med., 62, 436-439 (1996)   DOI   ScienceOn
2 Rosen, D.R., Jakobisiak, M., Hartmann-Petersen, R., Ortega, S. and Johnston, D.J.: Mutations with familial amyotrophic lateral sclerosis. Nature 362, 59-62 (1993)   DOI   ScienceOn
3 Ro, H.S., Ko, W.K., Yang, H.O., Park, K.K., Cho, Y.H., Lee, Y.E. and Park, H.S.: Isolation of hyperlipidemic substances from methanol extract of Paeoniae radix. J. Kor. Pharm. Sci., 29, 55-60 (1999)
4 Kinght, J. A.: The process and theories of aging. Ann. Clin. Lab. Sci. 25, 1-12 (1995)
5 Beauchamp, C. and Fridovich, I.: Superoxide dismutase : improved assays and an assay applicable to acrylamide gel. Anal Biochem. 44, 276-287 (1971)   DOI   ScienceOn
6 Gabriel, L.P. and William, R., Hewitt.: Principles and Methods of Toxicology. Raben Press, 407-445 (1982)
7 Gutteridge, J.M.C., Beard, A.P.C. and Quinlan, G.J.: Superoxide-dependent lipid peroxidation. Problem with the use of catalase as a specific probe for Fenton-derived hydroxyl radicals. Biochem. Biophys. Res. Commun. 117, 901-907 (1983)   DOI   ScienceOn
8 Fridovich, I.: Biologic effects of the superoxide radicals. Arch. Biochem. Biophysm 247, 1-15 (1986)   DOI   ScienceOn
9 Charles, A.J.: Immunobiology, 5th, Life Science Publication Co, Seoul, pp.76-77 (2002)
10 Charles, A.J.: Immunobiology, 5th, Life Science Publication Co, Seoul, pp.350-352 (2002)
11 Park, M.K., Lee, Y.J., and Kang, E.S.: Hepatoprotective effect of Cheonnyuncho (Opuntia humifusa) extract in rats treated carbon tetrachloride. Korean J. Food. Sci. Technol. 37, 822-826 (2005)   과학기술학회마을
12 생약교재편찬위원회: 생약학. 동명사, 서울, pp.372-373 (2000)
13 Kang, S.S., Kim, J.S., Kim, E.M. and Yun-Choi, H.S.: Platelet anti-aggregation of Paeony root. Kor. J. Pharmacogn., 22, 215-218 (1991)   과학기술학회마을
14 Yosjikawa, T., Murakami, M., Yoshida, N., Seto, O. and Kondo, M.: Effects of superoxide dimutase and catalase on disseminated intravascular coagulation in rats. Thromb. Haemostas 50, 869-872 (1983)   DOI
15 Ha, B.J., Lee, S.H., Kim, H.J. and Lee, J.Y.: The role of salicornia herbacea in ovariectomy-induced oxidative stress. Biol. Pharm. Bull. 29, 1305-1309 (2006)   DOI   ScienceOn
16 Lawrence, R. A. and Burk, R.F.: Glutathione peroxidase activity in selenium-deficient rat liver. Biochem. Biophys. Res. Commun. 71, 952-958 (1976)   DOI   ScienceOn
17 조명행: 기초 독성학, 개정판, 영지문화사, 서울, pp.115-116 (2004)
18 Kim, Y.E., Lee, Y.C., Kim, H.K. and Kim, C.J.: Antioxidative effect of ethanol fraction for several Korean medicinal plant hot water extracts. Korean J. Food & Nutr., 10, 141-144 (1997)
19 Ro, H.S., Ko, W.K., Park, K.K., Cho, Y.H. and Park, H.S.: Antihyperlipidemic effects of Bupleuri radix, Paeoniae radix and Uncariae ramulus et Uncus on experimental hyperlipidemia in rats. J. Applied. Pharm., 5, 43-47 (1997)
20 Aebi, H.: Catalase in vitro, Methods. Enzymology. 105, 121-126 (1984)   DOI
21 Halliwell, B.: How to charactrize a biological antioxidant. Free Radic. Res. Commun. 9, 1-32 (1990)   DOI
22 Ro, H.S., Ko, W.K., Yang, H.O., Park, K.K., Cho, Y.H. and Park, H.S.: Effect of several solvent extracts from Paeoniae radix on experimental hyperlipidemia in rats. J. Kor. Pharm. Sci., 27, 145-151 (1997)
23 Bang, M.H., Song, J.C., Lee, S.Y., Park, N.K. and Baek, N.I.: Isolation and structure determination of antioxidants from the root of Paeonia lactiflora. J. Korean Soc. Agric. Chem. Biotechnol., 42, 170-175 (1999)   과학기술학회마을
24 Liu, J.: Effect of Paeonia ovovata 801 on metabolism of thromboxane B2 and arachidonic acid and platelet aggregation in patients with coronary heart disease and cerebral thrombosis. Chung-Huai. Chih., 63, 477-481 (1983)
25 Kim, I.D., Kang, K.S., Kwon, R. H., Ha, B.J.: Anti-oxidative effects of Rubus coreanum Miquel extract on hepatic injury induced by lipopolysaccharide. Korean soc. environ. toxicol. 23, 347-352 (2007)   과학기술학회마을   DOI
26 Lowry, O.H., Rosenbrough, N.J., Farr, A.S. and Randall, R.J: Protein Measurenment with Folin phenol reagent. J. Biol.Chem. 193, 256-261 (1951)
27 Tanaka, T., Kataoka, M., Tsuboi, N. and Kouno, I.: New monoterpene glycoside esters and phenolic constituents of Paeoniae Radix, and increase of water solubility of proanthocyanidins in the presence of paeoniflorin. Chem. Pharm. Bull., 48, 201-207 (2000)   DOI   ScienceOn
28 Dantzer, R., Bluthe, R.M., Gheusi, G., Cremona, S., Laye, S., Parnet, P. and Kelley, K.W. : Molecular basis of sickness behavior. Annals N. Y. Acad. Sci. 856, 126-134 (1998)
29 McPhalen, C.A., Vincent, M.G. and Jansonius, J.N.: X-ray structure refinement and comparison of three forms of mitochondrial aspartate aminotransferase. J. Mol. Biol. 225, 495-517 (1992)   DOI
30 Tainer, J.A., Getzoff, E.D., Richardson, J.S. and Richardson, D.C.: Structure and mechanism of Cu, Zn, superoxide dismutase. Nature 306, 274-287 (1983)   DOI   ScienceOn
31 Von, S.: The Chemical Basis of Radiation of biology. Tylor and Francis, 3rd Ed. London, pp.31-33. (1987)