• Molecules and Cells
• /
• 제42권3호
• /
• pp.252-261
• /
• 2019

The omega-3 fatty acid docosahexaenoic acid (DHA) is known to induce apoptosis and cell cycle arrest via the induction of reactive oxygen species (ROS) production and endoplasmic reticulum (ER) stress in many types of cancers. However, the roles of DHA in drug-resistant cancer cells have not been elucidated. In this study, we investigated the effects of DHA in cisplatin-resistant gastric cancer SNU-601/cis2 cells. DHA was found to induce ROS-dependent apoptosis in these cells. The inositol 1,4,5-triphosphate receptor ($IP_3R$) blocker 2-aminoethyl diphenylboninate (2-APB) reduced DHA-induced ROS production, consequently reducing apoptosis. We also found that G-protein-coupled receptor 120 (GPR120), a receptor of long-chain fatty acids, is expressed in SNU-601/cis2 cells, and the knockdown of GPR120 using specific shRNAs alleviated DHA-mediated ROS production and apoptosis. GPR120 knockdown reduced the expression of ER stress response genes, similar to the case for the pre-treatment of the cells with N-acetyl-L-cysteine (NAC), an ROS scavenger, or 2-APB. Indeed, the knockdown of C/EBP homologous protein (CHOP), a transcription factor that functions under ER stress conditions, markedly reduced DHA-mediated apoptosis, indicating that CHOP plays an essential role in the anti-cancer activity of DHA. These results suggest that GPR120 mediates DHA-induced apoptosis by regulating $IP_3R$, ROS, and ER stress levels in cisplatin-resistant cancer cells, and that GPR120 is an effective chemotherapeutic target for cisplatin resistance.

• Biomolecules & Therapeutics
• /
• 제29권1호
• /
• pp.22-30
• /
• 2021

Till the 21st century, fatty acids were considered as merely building blocks for triglycerides, phospholipids, or cholesteryl esters. However, the discovery of G protein-coupled receptors (GPCRs) for free fatty acids at the beginning of the 21st century challenged that idea and paved way for a new field of research, merged into the field of receptor pharmacology for intercellular lipid mediators. Among the GPCRs for free fatty acids, free fatty acid receptor 4 (FFA4, also known as GPR120) recognizes long-chain polyunsaturated fatty acids such as DHA and EPA. It is significant in drug discovery because it regulates obesity-induced metaflammation and GLP-1 secretion. Our study reviews information on newly developed FFA4 agonists and their application in pathophysiologic studies and drug discovery. It also offers a potency comparison of the FFA4 agonists in an AP-TGF-α shedding assay.

• 헤리티지:역사와 과학
• /
• 제47권4호
• /
• pp.120-135
• /
• 2014

최근의 백제사찰 재조사에서 기존 가람의 양식과는 다른 가람배치 양상이 확인되면서 금강사지의 가람에 대하여 다시금 조사의 필요성을 가지게 한다. 본 연구는 GPR탐사법을 이용하여 금강사지의 잔존 유구 및 사역전체의 건물배치 양상을 확인하고자 하였으며, GIS기술을 이용하여 항공사진을 분석하고 수치지형도를 활용한 토양유실량(RUSLE)을 분석하여 금강사지의 입지와 지형변화를 파악하고자 하였다. GPR탐사 결과, 사역의 북편과 서편에서 기 발굴조사에서 미처 확인되지 않았던 건물지가 추가로 확인되어 사역의 범위는 추정 가람보다 더 넓었을 것으로 보인다. 또한 금강사지는 하안 단구면에 위치하고 있고 토양유실량이 매우 적은 안정된 지형장에 위치하며 자연발생적인 배수체계를 기반으로 하는 최적의 입지를 보이고 있음을 알 수 있었다.

• 한국식품영양학회지
• /
• 제25권4호
• /
• pp.850-854
• /
• 2012

This study is carried out in order to develop food materials for the promotion of rice consumption. In this study, we researched the physicochemical properties of extruded rice flour (ER) and gelatinization popped rice flour (GPR) by the extrusion process and by the popped method with milled rice and brown rice. The extrusion process used a couple of screw extruders. The extrusion parameter was kept constant at a feed moisture content of 25%, barrel temperature of $120^{\circ}C$ and screw speed of 400 rpm. GPR was prepared by batch popping machine after gelatinization of rice. In order to evaluate the physicochemical properties of ER and GPR, as well as the gelatinization rate, water soluble index (WSI), water adsorption index (WAI), DPPH radical scavenging activity and total dietary fiber content, the color value was carried out. The gelatinization rate was similar to 71.1~73.8%. Further, the results of WSI and WAI were increased at ER and GPR compared to the raw materials; ER was also higher than GPR. DPPH radical scavenging activity and total dietary fiber content showed a tendency to reduce after extrution and popping. As a result of the color value, the L value of ER decreased more than the raw materials, whereas GPR increased at a and b values.

• 한국철도학회논문집
• /
• 제11권3호
• /
• pp.334-341
• /
• 2008

자갈궤도는 압축성 재료인 자갈과 흙을 기초로 하여 건설되므로 공용 기간 중 승차감을 확보하고 수명연장을 위해서는 유지보수작업이 필수적이다. 현재 수행되고 있는 자갈궤도에 대한 유지보수 작업은 EM120 검측차를 이용한 궤도틀림 측정 결과로부터 결정된다. 궤도틀림은 궤도에 변형이 발생된 결과를 측정하는 것으로, 궤도틀림을 유발시키는 근본적인 원인을 평가해 볼 필요가 있다. 본 연구에서는 궤도틀림의 원인이 궤도를 지지하는 도상과 노반에 있다고 판단하고, 도상 및 노반의 싱태를 평가할 수 있는 비파괴탐사법 (GPR4과 PFWD)을 개발하고 이를 경부선 500m구간에 적용하였다. 개발 작용된 방법은 층 두께 평가법과 궤도수직강성 평가법 및 도상 노반 시추법이다. 도상 및 노반 상태평가 결과를 기 수행된 궤도틀림 검측결과 및 유지보수작업과 비교함으로서 불필요한 유지보수작업 구간을 평가하였다.

• 대한전자공학회:학술대회논문집
• /
• 대한전자공학회 2003년도 하계종합학술대회 논문집 I
• /
• pp.117-120
• /
• 2003

GPRS(General Packet Radio Service) 서비스는 circuit 기반의 GSM(Global System for Mobile Communication)의 기본 망구조를 이용하여 packet 서비스를 지원하도록 변형시킨 모델이다. 기본적으로 GPRS는 GSM의 많은 부분을 계승하여 서비스를 지원하고 있기 때문에 GSM이 가지고 있던 문제점을 그대로 이어받게 되었는데, 본 논문에서는 사용자 인증 프로시져등에서 문제시 될 수 있는 부분들에 대해 살펴보고, 이를 보완 할 수 있는 방법으로 공개키의 암호화 모드와 인증 모드를 사용한 인증 프로시져와 시스템 구조를 소개하고자 한다. 또한 공개키 구조의 인증을 사용함으로써 필요로 하게 되는 키 관리에 대한 방법을 제시하고 앞으로 해결해 나가야 될 문제에 대해 언급을 하고자 한다.

• 제어로봇시스템학회논문지
• /
• 제20권2호
• /
• pp.120-125
• /
• 2014

Conventional handheld metal detectors use a single induction coil to detect the metallic parts of explosive objects, and the detector generates an acoustic signal from its magnetic response to a metallic object so that an operator can confirm the existence of mines. Though metal detectors have very useful detection mechanisms to find mines, it is easy to cause a high false alarm ratio due to the detection of non-explosive metallic items such as cans, nails and other pieces of metal, etc. Also, because of the physical characteristic of a metal detector it is hard to detect non-metallic objects such as mines made of wood or plastic. Furthermore, the operator must move it to the left and right slowly and repeatedly to attain enough sensor signals to confirm the existence of mines using only a monotonous acoustic signal. To resolve the disadvantages of handheld detectors, many new approaches have been attempted, such as an arrayed detector and a visualization algorithm based on metal/non-metal sensor. In this paper, we introduce a visualization algorithm with a metal/non-metal complex sensor, an arrayed metal/non-metal sensor and the their testing and evaluation.

• 대한전기학회논문지:전력기술부문A
• /
• 제54권12호
• /
• pp.592-596
• /
• 2005

Recently, substations have been constructed in GIS (Gas Insulated Switchgear) and indoor type because of the increasing difficulties of securing locations. In case of urban substations, it is also common that the substations are exclusively fed by underground cables. Sometimes, the infrastructures in urban area, such as communication facilities, are located near substations to be constructed. In this paper, we examined such a case that a branch office building of KT(Korea Telecom) was located near 154 kV underground GIS substation under construction with about 100 meters of the lateral distance between two facilities. GPR (Ground Potential Rise) at the substation and the transferred earth potential at the KT building through the earth in SLG (Single-Line-to- Ground) fault condition were investigated based on a series of computer simulations. The calculated earth potential at a distance of 100 m from the substation was below 120 V which satisfying the limit value of 650 V.

• Journal of Ginseng Research
• /
• 제46권2호
• /
• pp.206-213
• /
• 2022

Ginsenoside Rb2 is an active protopanaxadiol-type saponin, widely existing in the stem and leave of ginseng. Rb2 has recently been the focus of studies for pharmaceutical properties. This paper provides an overview of the preclinical and clinical pharmacokinetics for Rb2, which exhibit poor absorption, rapid tissue distribution and slow excretion through urine. Pharmacological studies indicate a beneficial role of Rb2 in the prevention and treatment of diabetes, obesity, tumor, photoaging, virus infection and cardiovascular problems. The underlying mechanism is involved in an inhibition of oxidative stress, ROS generation, inflammation and apoptosis via regulation of various cellular signaling pathways and molecules, including AKT/SHP, MAPK, EGFR/SOX2, TGF-β1/Smad, SIRT1, GPR120/AMPK/HO-1 and NF-κB. This work would provide a new insight into the understanding and application of Rb2. However, its therapeutic effects have not been clinically evaluated. Further studies should be aimed at the clinical treatment of Rb2.